Rh5DG265200

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002418
Physical Location & Seq
Reverse (-)
32037284 .. 32037820
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG265200.1

Sequence Viewer

Length: 537 bp
ATGCATACTTACACGAAAATGGGTCAGACCCTTTTAACGGTTACAACACTCATTACAACTGTAACATTTACAGCCGCATTCACAATGCCTGGAGGCTACAACAATGATGCAGGCTCATCAGATCGAGGGCTAGCTCTTCTACAGTCAAGCAAATATCTAAAACATTTCATAATCTTCGACGCTGTTGCTATGACTTGCTCAATTAGTGCAGCTTGCCTCCTATTTTGGGGAGCTGTTAAAAGCAAGGGGTGCTATCTTTACTATTTCACAAGTGCCGCTGCTCTTACATATATTGCTCTACTATCGACTGCAGGAGCATTCACAACAGGAATTAAAGCTGTGCTGCCCCATCAGCAATACGTGGACATCATGATACTGGGAGTGGATGTTGCTTTTCACATCAGTACCTTCTTGTTTCTTTTTCATTTGGTTAAAATGTTTTCCATCCCTGAAGCCTGTAGACTCTTGTTTTCTCATCACTGTATCAAGTCCAAAAGGGCTAAGATACCAAAATGGAGAAGAAGTACATATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

178

Amino Acids

19.78

Weight (kDa)

9.54

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 3 - 112 4.7e-24 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 460
AciI CCGC 2 cut(s) 75, 276
AcuI CTGAAG 1 cut(s) 471
AfaI GTAC 2 cut(s) 406, 526
AfiI CCNNNNNNNGG 2 cut(s) 37, 226
AjnI CCWGG 1 cut(s) 88
AluBI AGCT 4 cut(s) 134, 212, 233, 338
AluI AGCT 4 cut(s) 134, 212, 233, 338
ApeKI GCWGC 3 cut(s) 209, 278, 343
AsuNHI GCTAGC 1 cut(s) 130
BaeI ACNNNNGTAYC 2 cut(s) 388, 421
BbvI GCAGC 3 cut(s) 221, 265, 330
BccI CCATC 2 cut(s) 357, 452
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BciT130I CCWGG 1 cut(s) 90
BfaI CTAG 1 cut(s) 131
BfmI CTRYAG 3 cut(s) 140, 309, 457
BisI GCNGC 5 cut(s) 75, 210, 276, 279, 344
BlsI GCNGC 5 cut(s) 76, 211, 277, 280, 345
Bme1390I CCNGG 1 cut(s) 90
BmrFI CCNGG 1 cut(s) 90
BmrI ACTGGG 1 cut(s) 386
BmsI GCATC 1 cut(s) 97
BmtI GCTAGC 1 cut(s) 134
BmuI ACTGGG 1 cut(s) 386
BpmI CTGGAG 1 cut(s) 111
BsaAI YACGTR 1 cut(s) 361
BsaXI ACNNNNNCTCC 3 cut(s) 372, 402, 508
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 226
Bse1I ACTGG 1 cut(s) 381
BseBI CCWGG 1 cut(s) 90
BseGI GGATG 2 cut(s) 391, 444
BseLI CCNNNNNNNGG 2 cut(s) 37, 226
BseNI ACTGG 1 cut(s) 381
BseXI GCAGC 3 cut(s) 221, 265, 330
BsgI GTGCAG 1 cut(s) 228
BslI CCNNNNNNNGG 2 cut(s) 37, 226
BsmI GAATGC 2 cut(s) 77, 317
Bsp143I GATC 1 cut(s) 121
BspACI CCGC 2 cut(s) 75, 276
BspHI TCATGA 1 cut(s) 369
BspMAI CTGCAG 1 cut(s) 313
BspOI GCTAGC 1 cut(s) 134
BspQI GCTCTTC 1 cut(s) 141
BsrI ACTGG 1 cut(s) 381
BssMI GATC 1 cut(s) 121
Bst2UI CCWGG 1 cut(s) 90
Bst4CI ACNGT 4 cut(s) 40, 61, 144, 482
Bst6I CTCTTC 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 249
BstBAI YACGTR 1 cut(s) 361
BstC8I GCNNGC 3 cut(s) 112, 132, 214
BstDEI CTNAG 1 cut(s) 501
BstF5I GGATG 2 cut(s) 391, 444
BstKTI GATC 1 cut(s) 124
BstMBI GATC 1 cut(s) 121
BstMWI GCNNNNNNNGC 2 cut(s) 249, 352
BstNI CCWGG 1 cut(s) 90
BstSCI CCNGG 1 cut(s) 88
BstSFI CTRYAG 3 cut(s) 140, 309, 457
BstV1I GCAGC 3 cut(s) 221, 265, 330
BtsCI GGATG 2 cut(s) 391, 444
BtsIMutI CAGTG 1 cut(s) 478
Cac8I GCNNGC 3 cut(s) 112, 132, 214
CciI TCATGA 1 cut(s) 369
CseI GACGC 1 cut(s) 188
Csp6I GTAC 2 cut(s) 405, 525
CviAII CATG 1 cut(s) 370
CviQI GTAC 2 cut(s) 405, 525
DdeI CTNAG 1 cut(s) 501
DpnI GATC 1 cut(s) 123
DpnII GATC 1 cut(s) 121
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
Eco57I CTGAAG 1 cut(s) 471
EcoRII CCWGG 1 cut(s) 88
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 1 cut(s) 373
FaiI YATR 7 cut(s) 6, 170, 191, 289, 291, 371, 529
FatI CATG 1 cut(s) 369
FblI GTMKAC 1 cut(s) 460
Fnu4HI GCNGC 5 cut(s) 75, 210, 276, 279, 344
FokI GGATG 2 cut(s) 398, 431
Fsp4HI GCNGC 5 cut(s) 75, 210, 276, 279, 344
FspBI CTAG 1 cut(s) 131
GluI GCNGC 5 cut(s) 75, 210, 276, 279, 344
GsuI CTGGAG 1 cut(s) 111
HgaI GACGC 1 cut(s) 188
Hin1II CATG 1 cut(s) 373
HinfI GANTC 1 cut(s) 462
Hpy166II GTNNAC 2 cut(s) 364, 461
Hpy188I TCNGA 2 cut(s) 27, 121
Hpy188III TCNNGA 1 cut(s) 370
Hpy8I GTNNAC 2 cut(s) 364, 461
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 4 cut(s) 40, 61, 144, 482
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 4 cut(s) 4, 110, 209, 311
HpyF10VI GCNNNNNNNGC 2 cut(s) 249, 352
HpyF3I CTNAG 1 cut(s) 501
HpySE526I ACGT 1 cut(s) 360
Hsp92II CATG 1 cut(s) 373
Kzo9I GATC 1 cut(s) 121
LguI GCTCTTC 1 cut(s) 141
LmnI GCTCC 2 cut(s) 230, 314
LpnPI CCDG 8 cut(s) 75, 96, 102, 297, 312, 362, 462, 469
Lsp1109I GCAGC 3 cut(s) 221, 265, 330
LweI GCATC 1 cut(s) 97
MaeI CTAG 1 cut(s) 131
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 2 cut(s) 40, 61
MalI GATC 1 cut(s) 123
MboI GATC 1 cut(s) 121
MboII GAAGA 3 cut(s) 128, 166, 531
MluCI AATT 2 cut(s) 201, 330
MlyI GAGTC 1 cut(s) 456
MnlI CCTC 3 cut(s) 86, 119, 227
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 4 cut(s) 35, 237, 333, 432
MslI CAYNNNNRTG 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 278
MspR9I CCNGG 1 cut(s) 90
Mva1269I GAATGC 2 cut(s) 77, 317
MvaI CCWGG 1 cut(s) 90
MwoI GCNNNNNNNGC 2 cut(s) 249, 352
NdeII GATC 1 cut(s) 121
NheI GCTAGC 1 cut(s) 130
NlaIII CATG 1 cut(s) 373
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 369
PciSI GCTCTTC 1 cut(s) 141
PctI GAATGC 2 cut(s) 77, 317
PkrI GCNGC 5 cut(s) 76, 211, 277, 280, 345
PleI GAGTC 1 cut(s) 456
PpsI GAGTC 1 cut(s) 456
Ppu21I YACGTR 1 cut(s) 361
Psp6I CCWGG 1 cut(s) 88
PspGI CCWGG 1 cut(s) 88
PstI CTGCAG 1 cut(s) 313
RsaI GTAC 2 cut(s) 406, 526
RsaNI GTAC 2 cut(s) 405, 525
RseI CAYNNNNRTG 1 cut(s) 17
SapI GCTCTTC 1 cut(s) 141
SaqAI TTAA 4 cut(s) 35, 237, 333, 432
SatI GCNGC 5 cut(s) 75, 210, 276, 279, 344
Sau3AI GATC 1 cut(s) 121
SchI GAGTC 1 cut(s) 456
ScrFI CCNGG 1 cut(s) 90
SetI ASST 6 cut(s) 136, 214, 235, 340, 363, 410
SfaNI GCATC 1 cut(s) 97
SfcI CTRYAG 3 cut(s) 140, 309, 457
SmiMI CAYNNNNRTG 1 cut(s) 17
Sse9I AATT 2 cut(s) 201, 330
SsiI CCGC 2 cut(s) 75, 276
SspMI CTAG 1 cut(s) 131
StyD4I CCNGG 1 cut(s) 88
TaaI ACNGT 4 cut(s) 40, 61, 144, 482
TaiI ACGT 1 cut(s) 363
TaqI TCGA 3 cut(s) 124, 177, 305
TasI AATT 2 cut(s) 201, 330
TatI WGTACW 1 cut(s) 524
TauI GCSGC 2 cut(s) 77, 278
Tru1I TTAA 4 cut(s) 35, 237, 333, 432
Tru9I TTAA 4 cut(s) 35, 237, 333, 432
TscAI CASTG 1 cut(s) 485
TseI GCWGC 3 cut(s) 209, 278, 343
TspDTI ATGAA 2 cut(s) 157, 413
TspRI CASTG 1 cut(s) 485
XmiI GTMKAC 1 cut(s) 460
XspI CTAG 1 cut(s) 131
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.