RchiOBHm_Chr5g0037621

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002418
Physical Location & Seq
Reverse (-)
32237412 .. 32239718
2307 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31632

Sequence Viewer

Length: 1689 bp
ATGGATCCAAGGCTCTACAAATCTGCAAAATCAGGGGATGTTTGCTTCCTCAGAAAACTTCTTAGTGATAATCCTAGCCTTCTATATAAACTTACACCTCGAGAAAACACAACCCTCCATATAGCTGTGCAATTCGGACACGAAAATGTCGCGGCTGAGATTTATAGCAGATCCAGGTCACTTCTCAAACAGCAAAACTTAGATGGAGATACACCTCTACATGTGGCTGCAAGGTTTGGTCACTTCTCTATTGTCAACTATCTGGTTAGAGATATAATTTCATCAGTGTCAGAGGTAGACATGGGGCTTGAGACCCTGAGAATTAGAAATAGGGGGAACAGCACAGTATTACACGAAGCTTCAAGGAATGGTCACTATAAAGTGGTCGAGTTCTTAATCAAAGTTGATCCCAACTTGGCTTCTGTTGAAAATGATGAGGGTGAGTCTCCATTGTATCTGGCCGCAAGAGGCGGAATGTTAGAGACTGTGAATCAAATTATAAGGACAAATCCGTCATCAGCTCATGGTGGTTCAGATGGCCAAACAGCTTTGCATGCTGCTGTGGTTGAGAGGCATATTGGTGTCATTGAAGCTCTGCTAGCATTCAAACAAGAACTTGGCAAAGAAGCTGATCGACAAGGCCGGACTCCTCTCTACTATGCAGCATCCCTAGGTGACCACAGAACAGTTCAGCGATTACTAGAACTTGATACTTCTACTGCATATGTCTTGGATAAAGATGGTCGTTCACCAATTCATGTTGCAGCTAGGAATGGTCATACTGGTGTGATTAGAGAGATTATCCAACATTGCCCAGATTCCGGAGAACTTATTGACCCTTATGGACAGAATGCTCTTCATATTGCTATTTCCAGTGGACAAGCTAATGTTGTCACATATATATTAGAAACACCAGAACTAGAAGGGTTGATAAATCAGTCTGATATTGATGGAAACACGCCTTTGCATCTAGCAGCGATTGCAAGAAAAACTTGGATTCTTTGGTACTTGATGTGGGATGGAAGAGTAAATCAAAGATCCAAGAACAAATATGGCCAAACAGCATTTGACAATGATAGGTCAATGAAGAAATCTAGTATTACATCTCCCATGATTTCAGCAAGAGCGGACCACGAAGAAGCAATTTCAGCAATGCAAACTTACAAGAAAATGGGTCAGACCCTTTTAACGGTTACAACACTCATTACAACTGTAACATTTACAGCCGCATTCACAATGCCTGGAGGCTACAACAATGATGCAGGCTCATCAGATCGAGGGCTAGCTCTTCTACAGTCGAGCAAATATCTAAAACATTTCATAATCTTCGACGCTGTTGCTATGACTTGCTCAATTAGTGCAGCTTGCCTCCTATTTTGGGGAGCTGTTAAAAGCAAGGGGTGCTATCTTTACTATTTCACAAGTGCCGCTGCTCTTACATATATTGCTCTACTATCGACTGCAGGAGCATTCACAACAGGAATTAAAGCTGTGCTGCCCCATCAGCAATACGTGGACATCATGATACTGGGAGTGGATGTTGCTTTTCACATCAGTACCTTCTTGTTTCTTTTTCATTTGGTTAAAATGTTTTCCATCCCTGAAGCCTGTAGACTCTTGTTTTCTCATCACTGTATCAAGTCCAAAAGGGCTAAGATACAAAAATGGAGAAGAAGTACATATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

562

Amino Acids

62.31

Weight (kDa)

8.74

Isoelectric Point (pI)

33.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 7 - 90 2e-10 Ankyrin repeats (3 copies)
Ank PF00023 68 - 90 9.5e-06 Ankyrin repeat
Ank_2 PF12796 110 - 167 1e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 151 - 236 8.3e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 218 - 264 1.4e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 225 - 303 6.1e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 276 - 325 2.6e-06 Ankyrin repeats (many copies)
PGG PF13962 387 - 496 1.7e-23 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 500
AasI GACNNNNNNGTC 1 cut(s) 511
AccBSI CCGCTC 1 cut(s) 1127
AccI GTMKAC 2 cut(s) 297, 1612
AccII CGCG 1 cut(s) 152
AccIII TCCGGA 1 cut(s) 821
AciI CCGC 6 cut(s) 152, 462, 471, 1127, 1227, 1428
AclWI GGATC 4 cut(s) 12, 165, 401, 1032
AcoI YGGCCR 3 cut(s) 459, 538, 1054
AcuI CTGAAG 1 cut(s) 1623
AfaI GTAC 3 cut(s) 1007, 1558, 1678
AfiI CCNNNNNNNGG 3 cut(s) 821, 1189, 1378
AflIII ACRYGT 1 cut(s) 220
AgsI TTSAA 4 cut(s) 363, 428, 590, 607
AjnI CCWGG 2 cut(s) 173, 1240
Alw26I GTCTC 3 cut(s) 305, 450, 476
AlwI GGATC 4 cut(s) 12, 165, 401, 1032
Ama87I CYCGRG 1 cut(s) 99
Aor13HI TCCGGA 1 cut(s) 821
AoxI GGCC 4 cut(s) 459, 538, 640, 1054
ApeKI GCWGC 8 cut(s) 227, 557, 662, 764, 974, 1361, 1430, 1495
AspA2I CCTAGG 1 cut(s) 670
AspS9I GGNCC 1 cut(s) 1129
AsuHPI GGTGA 3 cut(s) 452, 686, 741
AsuNHI GCTAGC 2 cut(s) 598, 1282
AvaI CYCGRG 1 cut(s) 99
AvaII GGWCC 1 cut(s) 1129
AvrII CCTAGG 1 cut(s) 670
BaeI ACNNNNGTAYC 2 cut(s) 1540, 1573
BalI TGGCCA 2 cut(s) 540, 1056
BamHI GGATCC 1 cut(s) 4
BbvI GCAGC 8 cut(s) 214, 544, 674, 776, 986, 1373, 1417, 1482
BccI CCATC 7 cut(s) 197, 530, 734, 944, 1013, 1509, 1604
BcgI CGANNNNNNTGC 2 cut(s) 1319, 1353
BciT130I CCWGG 2 cut(s) 175, 1242
BcoDI GTCTC 3 cut(s) 305, 450, 476
BfaI CTAG 9 cut(s) 75, 599, 671, 701, 768, 920, 971, 1095, 1283
BfmI CTRYAG 3 cut(s) 1292, 1461, 1609
BlnI CCTAGG 1 cut(s) 670
Bme1390I CCNGG 2 cut(s) 175, 1242
Bme18I GGWCC 1 cut(s) 1129
BmeT110I CYCGRG 1 cut(s) 99
BmgT120I GGNCC 1 cut(s) 1129
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 175, 1242
BmrI ACTGGG 1 cut(s) 1538
BmsI GCATC 3 cut(s) 674, 976, 1249
BmtI GCTAGC 2 cut(s) 602, 1286
BmuI ACTGGG 1 cut(s) 1538
BpmI CTGGAG 1 cut(s) 1263
BpuEI CTTGAG 1 cut(s) 329
BsaAI YACGTR 1 cut(s) 1513
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 2 cut(s) 8, 670
BsaWI WCCGGW 1 cut(s) 821
BsaXI ACNNNNNCTCC 3 cut(s) 1524, 1554, 1660
Bsc4I CCNNNNNNNGG 3 cut(s) 821, 1189, 1378
Bse1I ACTGG 3 cut(s) 787, 873, 1533
Bse3DI GCAATG 2 cut(s) 808, 1158
BseAI TCCGGA 1 cut(s) 821
BseBI CCWGG 2 cut(s) 175, 1242
BseDI CCNNGG 2 cut(s) 8, 670
BseGI GGATG 5 cut(s) 43, 665, 1024, 1543, 1596
BseLI CCNNNNNNNGG 3 cut(s) 821, 1189, 1378
BseMI GCAATG 2 cut(s) 808, 1158
BseMII CTCAG 3 cut(s) 64, 147, 308
BseNI ACTGG 3 cut(s) 787, 873, 1533
BseRI GAGGAG 1 cut(s) 639
BseXI GCAGC 8 cut(s) 214, 544, 674, 776, 986, 1373, 1417, 1482
BsgI GTGCAG 1 cut(s) 1380
Bsh1236I CGCG 1 cut(s) 152
BshFI GGCC 4 cut(s) 461, 540, 642, 1056
BsiHKCI CYCGRG 1 cut(s) 99
BsiSI CCGG 2 cut(s) 643, 822
BslI CCNNNNNNNGG 3 cut(s) 821, 1189, 1378
BsmAI GTCTC 3 cut(s) 305, 450, 476
BsmI GAATGC 4 cut(s) 602, 856, 1229, 1469
BsnI GGCC 4 cut(s) 461, 540, 642, 1056
Bso31I GGTCTC 1 cut(s) 305
BsoBI CYCGRG 1 cut(s) 99
Bsp13I TCCGGA 1 cut(s) 821
Bsp143I GATC 6 cut(s) 4, 170, 406, 631, 1037, 1273
BspACI CCGC 6 cut(s) 152, 462, 471, 1127, 1227, 1428
BspANI GGCC 4 cut(s) 461, 540, 642, 1056
BspCNI CTCAG 3 cut(s) 63, 148, 309
BspEI TCCGGA 1 cut(s) 821
BspFNI CGCG 1 cut(s) 152
BspHI TCATGA 1 cut(s) 1521
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 1465
BspOI GCTAGC 2 cut(s) 602, 1286
BspPI GGATC 4 cut(s) 12, 165, 401, 1032
BspQI GCTCTTC 2 cut(s) 861, 1293
BspTNI GGTCTC 1 cut(s) 305
BsrBI CCGCTC 1 cut(s) 1127
BsrDI GCAATG 2 cut(s) 808, 1158
BsrI ACTGG 3 cut(s) 787, 873, 1533
BssECI CCNNGG 2 cut(s) 8, 670
BssMI GATC 6 cut(s) 4, 170, 406, 631, 1037, 1273
BssT1I CCWWGG 2 cut(s) 8, 670
Bst2UI CCWGG 2 cut(s) 175, 1242
Bst4CI ACNGT 7 cut(s) 346, 487, 688, 1192, 1213, 1296, 1634
Bst6I CTCTTC 3 cut(s) 861, 1018, 1293
BstAPI GCANNNNNTGC 2 cut(s) 980, 1401
BstBAI YACGTR 1 cut(s) 1513
BstC8I GCNNGC 5 cut(s) 555, 600, 1264, 1284, 1366
BstDEI CTNAG 6 cut(s) 50, 62, 156, 199, 317, 1653
BstEII GGTNACC 1 cut(s) 674
BstF5I GGATG 5 cut(s) 43, 665, 1024, 1543, 1596
BstFNI CGCG 1 cut(s) 152
BstKTI GATC 6 cut(s) 7, 173, 409, 634, 1040, 1276
BstMAI GTCTC 3 cut(s) 305, 450, 476
BstMBI GATC 6 cut(s) 4, 170, 406, 631, 1037, 1273
BstMWI GCNNNNNNNGC 6 cut(s) 554, 599, 980, 1148, 1401, 1504
BstNI CCWGG 2 cut(s) 175, 1242
BstNSI RCATGY 2 cut(s) 224, 557
BstPI GGTNACC 1 cut(s) 674
BstSCI CCNGG 2 cut(s) 173, 1240
BstSFI CTRYAG 3 cut(s) 1292, 1461, 1609
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 8 cut(s) 214, 544, 674, 776, 986, 1373, 1417, 1482
BstX2I RGATCY 3 cut(s) 4, 170, 1037
BstYI RGATCY 3 cut(s) 4, 170, 1037
BsuRI GGCC 4 cut(s) 461, 540, 642, 1056
BtsCI GGATG 5 cut(s) 43, 665, 1024, 1543, 1596
BtsIMutI CAGTG 3 cut(s) 291, 880, 1630
Cac8I GCNNGC 5 cut(s) 555, 600, 1264, 1284, 1366
CciI TCATGA 1 cut(s) 1521
Cfr13I GGNCC 1 cut(s) 1129
CseI GACGC 1 cut(s) 1340
Csp6I GTAC 3 cut(s) 1006, 1557, 1677
CviAII CATG 7 cut(s) 221, 301, 524, 554, 758, 1111, 1522
CviQI GTAC 3 cut(s) 1006, 1557, 1677
DdeI CTNAG 6 cut(s) 50, 62, 156, 199, 317, 1653
DpnI GATC 6 cut(s) 6, 172, 408, 633, 1039, 1275
DpnII GATC 6 cut(s) 4, 170, 406, 631, 1037, 1273
DrdI GACNNNNNNGTC 1 cut(s) 511
DseDI GACNNNNNNGTC 1 cut(s) 511
EaeI YGGCCR 3 cut(s) 459, 538, 1054
Eam1104I CTCTTC 3 cut(s) 861, 1018, 1293
EarI CTCTTC 3 cut(s) 861, 1018, 1293
EciI GGCGGA 1 cut(s) 486
Eco130I CCWWGG 2 cut(s) 8, 670
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 1129
Eco57I CTGAAG 1 cut(s) 1623
Eco88I CYCGRG 1 cut(s) 99
Eco91I GGTNACC 1 cut(s) 674
EcoO65I GGTNACC 1 cut(s) 674
EcoRII CCWGG 2 cut(s) 173, 1240
EcoT14I CCWWGG 2 cut(s) 8, 670
ErhI CCWWGG 2 cut(s) 8, 670
FaeI CATG 7 cut(s) 224, 304, 527, 557, 761, 1114, 1525
FalI AAGNNNNNCTT 2 cut(s) 976, 1008
FatI CATG 7 cut(s) 220, 300, 523, 553, 757, 1110, 1521
FauNDI CATATG 1 cut(s) 724
FblI GTMKAC 2 cut(s) 297, 1612
FokI GGATG 5 cut(s) 50, 652, 1031, 1550, 1583
FspBI CTAG 9 cut(s) 75, 599, 671, 701, 768, 920, 971, 1095, 1283
GsuI CTGGAG 1 cut(s) 1263
HaeIII GGCC 4 cut(s) 461, 540, 642, 1056
HapII CCGG 2 cut(s) 643, 822
HgaI GACGC 1 cut(s) 1340
Hin1II CATG 7 cut(s) 224, 304, 527, 557, 761, 1114, 1525
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 357
HinfI GANTC 6 cut(s) 443, 490, 646, 818, 997, 1614
HpaII CCGG 2 cut(s) 643, 822
HphI GGTGA 3 cut(s) 452, 686, 741
Hpy166II GTNNAC 6 cut(s) 256, 298, 749, 878, 1516, 1613
Hpy188I TCNGA 7 cut(s) 53, 137, 292, 535, 943, 1179, 1273
Hpy188III TCNNGA 3 cut(s) 101, 822, 1522
Hpy8I GTNNAC 6 cut(s) 256, 298, 749, 878, 1516, 1613
Hpy99I CGWCG 1 cut(s) 1334
HpyAV CCTTC 3 cut(s) 89, 917, 1570
HpyCH4III ACNGT 7 cut(s) 346, 487, 688, 1192, 1213, 1296, 1634
HpyCH4IV ACGT 1 cut(s) 1512
HpyF10VI GCNNNNNNNGC 6 cut(s) 554, 599, 980, 1148, 1401, 1504
HpyF3I CTNAG 6 cut(s) 50, 62, 156, 199, 317, 1653
HpySE526I ACGT 1 cut(s) 1512
Hsp92II CATG 7 cut(s) 224, 304, 527, 557, 761, 1114, 1525
Kpn2I TCCGGA 1 cut(s) 821
Kzo9I GATC 6 cut(s) 4, 170, 406, 631, 1037, 1273
LguI GCTCTTC 2 cut(s) 861, 1293
LmnI GCTCC 2 cut(s) 1382, 1466
Lsp1109I GCAGC 8 cut(s) 214, 544, 674, 776, 986, 1373, 1417, 1482
LweI GCATC 3 cut(s) 674, 976, 1249
MaeI CTAG 9 cut(s) 75, 599, 671, 701, 768, 920, 971, 1095, 1283
MaeII ACGT 1 cut(s) 1512
MaeIII GTNAC 7 cut(s) 177, 239, 371, 674, 892, 1192, 1213
MalI GATC 6 cut(s) 6, 172, 408, 633, 1039, 1275
MbiI CCGCTC 1 cut(s) 1127
MboI GATC 6 cut(s) 4, 170, 406, 631, 1037, 1273
MboII GAAGA 7 cut(s) 848, 1035, 1099, 1148, 1280, 1318, 1683
MflI RGATCY 3 cut(s) 4, 170, 1037
MlsI TGGCCA 2 cut(s) 540, 1056
MluCI AATT 8 cut(s) 131, 276, 321, 495, 753, 1143, 1353, 1482
MluNI TGGCCA 2 cut(s) 540, 1056
MlyI GAGTC 3 cut(s) 452, 640, 1608
MmeI TCCRAC 1 cut(s) 829
Mox20I TGGCCA 2 cut(s) 540, 1056
MroI TCCGGA 1 cut(s) 821
MscI TGGCCA 2 cut(s) 540, 1056
MseI TTAA 5 cut(s) 395, 1187, 1389, 1485, 1584
MslI CAYNNNNRTG 3 cut(s) 144, 579, 783
Msp20I TGGCCA 2 cut(s) 540, 1056
MspA1I CMGCKG 1 cut(s) 1430
MspI CCGG 2 cut(s) 643, 822
MspR9I CCNGG 2 cut(s) 175, 1242
Mva1269I GAATGC 4 cut(s) 602, 856, 1229, 1469
MvaI CCWGG 2 cut(s) 175, 1242
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 6 cut(s) 554, 599, 980, 1148, 1401, 1504
NdeI CATATG 1 cut(s) 724
NdeII GATC 6 cut(s) 4, 170, 406, 631, 1037, 1273
NheI GCTAGC 2 cut(s) 598, 1282
NlaIII CATG 7 cut(s) 224, 304, 527, 557, 761, 1114, 1525
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 5 cut(s) 177, 239, 371, 674, 892
NspI RCATGY 2 cut(s) 224, 557
PaeI GCATGC 1 cut(s) 557
PaeR7I CTCGAG 1 cut(s) 99
PagI TCATGA 1 cut(s) 1521
PciI ACATGT 1 cut(s) 220
PciSI GCTCTTC 2 cut(s) 861, 1293
PctI GAATGC 4 cut(s) 602, 856, 1229, 1469
PfeI GAWTC 3 cut(s) 490, 818, 997
PleI GAGTC 3 cut(s) 451, 640, 1608
PpsI GAGTC 3 cut(s) 451, 640, 1608
Ppu21I YACGTR 1 cut(s) 1513
PscI ACATGT 1 cut(s) 220
PsiI TTATAA 1 cut(s) 500
Psp6I CCWGG 2 cut(s) 173, 1240
PspEI GGTNACC 1 cut(s) 674
PspGI CCWGG 2 cut(s) 173, 1240
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 1129
PstI CTGCAG 1 cut(s) 1465
PsuI RGATCY 3 cut(s) 4, 170, 1037
RsaI GTAC 3 cut(s) 1007, 1558, 1678
RsaNI GTAC 3 cut(s) 1006, 1557, 1677
RseI CAYNNNNRTG 3 cut(s) 144, 579, 783
SapI GCTCTTC 2 cut(s) 861, 1293
SaqAI TTAA 5 cut(s) 395, 1187, 1389, 1485, 1584
Sau3AI GATC 6 cut(s) 4, 170, 406, 631, 1037, 1273
Sau96I GGNCC 1 cut(s) 1129
SchI GAGTC 3 cut(s) 452, 640, 1608
ScrFI CCNGG 2 cut(s) 175, 1242
SfaNI GCATC 3 cut(s) 674, 976, 1249
SfcI CTRYAG 3 cut(s) 1292, 1461, 1609
Sfr274I CTCGAG 1 cut(s) 99
SinI GGWCC 1 cut(s) 1129
SlaI CTCGAG 1 cut(s) 99
SmiMI CAYNNNNRTG 3 cut(s) 144, 579, 783
SmlI CTYRAG 2 cut(s) 99, 308
SmoI CTYRAG 2 cut(s) 99, 308
SphI GCATGC 1 cut(s) 557
Sse9I AATT 8 cut(s) 131, 276, 321, 495, 753, 1143, 1353, 1482
SsiI CCGC 6 cut(s) 152, 462, 471, 1127, 1227, 1428
SspMI CTAG 9 cut(s) 75, 599, 671, 701, 768, 920, 971, 1095, 1283
StyD4I CCNGG 2 cut(s) 173, 1240
StyI CCWWGG 2 cut(s) 8, 670
TaaI ACNGT 7 cut(s) 346, 487, 688, 1192, 1213, 1296, 1634
TaiI ACGT 1 cut(s) 1515
TaqI TCGA 7 cut(s) 100, 387, 634, 1276, 1298, 1329, 1457
TasI AATT 8 cut(s) 131, 276, 321, 495, 753, 1143, 1353, 1482
TatI WGTACW 1 cut(s) 1676
TauI GCSGC 4 cut(s) 155, 464, 1229, 1430
TfiI GAWTC 3 cut(s) 490, 818, 997
Tru1I TTAA 5 cut(s) 395, 1187, 1389, 1485, 1584
Tru9I TTAA 5 cut(s) 395, 1187, 1389, 1485, 1584
TscAI CASTG 3 cut(s) 291, 880, 1637
TseFI GTSAC 5 cut(s) 177, 239, 371, 674, 892
TseI GCWGC 8 cut(s) 227, 557, 662, 764, 974, 1361, 1430, 1495
Tsp45I GTSAC 5 cut(s) 177, 239, 371, 674, 892
TspDTI ATGAA 6 cut(s) 270, 746, 848, 1100, 1309, 1565
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 3 cut(s) 291, 880, 1637
VpaK11BI GGWCC 1 cut(s) 1129
XceI RCATGY 2 cut(s) 224, 557
XhoI CTCGAG 1 cut(s) 99
XmaJI CCTAGG 1 cut(s) 670
XmiI GTMKAC 2 cut(s) 297, 1612
XspI CTAG 9 cut(s) 75, 599, 671, 701, 768, 920, 971, 1095, 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.