Rh5BG256700

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002418
Physical Location & Seq
Reverse (-)
32102377 .. 32105363
2987 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG256700.1

Sequence Viewer

Length: 1728 bp
ATGTACTACTGTACCTGTGCCCAGAACAAATATACCATGGATCCAAGGCTCTACAAATCTGCAAAATCAGGGGATGTTTGCTTCCTCAGAAAACTTCTTAGTGATAATCCTAGCCTTCTATATAAACTTACACCTCGAGAAAACACAATCCTCCATATAGCTGCGCAATTCGGACACAAAAATGTCGTGGCTGAGATTTATAGCAGATCCGGGTCACTTCTCATACAGCAAAACTTAGATGGAGATACACCTCTACATGTGGCTGCAAGGTTTGGTCACTTCTCTATTGTCAACTATCTGGTTAGAGATATAATTTCATCAGTGTCAGGGGTAGACCTGGGGCTTGAGACTCTGAGAATTAGAAATAGGGGGAGCAACACAGTATTACACGAAGCTTCAAGGAATGGTCACTATAAAGTGGTCGAGTTCTTAATCAAAGTTGATCCTAACTTGGCTTCTGTTGAAAATGATGAGGGTGAGTCTCCATTGTATCTGGTCGCAAGAGGGGGAATGTTAGAGATTGTGAATCAAATTATAAGGACAACTCCGTCATCAGCTCATGGTGGTTCAGATGGCCAAACAGCTTTGCATGCTGCTGTGGTTGAGAGGCATATTGGTATCATGGAAGCTCTGCTAGCATTCAAGCAAGAACTAGTCAAAGAAGCTGATCGACAAGGCCGGACTCCTCTCTACTATGCAGCATCCCTAGGTGACCACAGAACAGTTCAGCGATTACTAGAACTTGATACTTCTACTGCATATGTCTTGGATATAGATGGTCGTTCACCAATTCATGTTGCAGCTAGGAATGGTCATACCGGTGTGATTAGAGAGATTATCCAACATTGCCCAGATTCGGGAGAACTTGTTAACCCTTATGGAGAGAATGCTCTTCATATTGCTATTTCCAGTGGACAAGCTAATGTTGTCACATATATATTAGAAACACCAGAACTAGAAGGGTTGATAAATCAGTCTGATATTGATGGAAACACGCCTTTGCATCTAGCAGCGATTGCAAGAAAAACTTGGATTCTTTGGTACTTGAGGTGGGATGGAAGAGTAAATCAAAGATCCAAGAACAAATATGGCCAAACAGCATTTGACAATGATAGGTCAATGAAGAAATCTAGTAATATTACATCTCCCATGATTTCAGCAAGAGCGGACCACGAAGAAGCAATTTCAGCAATGCAAACTTACAAGAAAATGGGTCAGACCCTTTTAACGGTTACAACACTCATTACAACTGTAACATTTACAGCCGCATTCACAATGCCTGGAGGCTACAACAATGATGCAGGCTTATCAGATCGAGGGCTAGCTCTTCTACAGTCGAGCAAATATCTAAAACATTTCATAATCTTCGACGCTGTTGCTATGACTTGCTCAATTAGTGCAGCTTGCCTCCTATTTTGGGGAGCTGTTAAAAGCAAGGGGTGCTATCTTTACTATTTCACAAGTGCCGCTGCTCTTACATATATTGCTCTACAATCGACTGCAGGAGCATTCACAACAGGAATTAAAGCTGCGCTGCCCCATCAGCAATACGTGAACATCATGATACTGGGAGTGGATGTTGCTTTTCACATCAGTGCCTTCTTGTTTCTTTTTCATTTGGTTAAAATGTTTTCCATCCCTGAAGCCTGTAGACTCTTGTTTTCTCATCACTGTATCAAGTCCAAAAGGGCTAAGATACAAAAATGGAGAAGAAGTACATATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

575

Amino Acids

63.78

Weight (kDa)

8.92

Isoelectric Point (pI)

37.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 19 - 101 1e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 51 - 101 1.2e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 123 - 179 2.2e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 129 - 213 3.3e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 163 - 248 9.5e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 232 - 276 6.8e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 256 - 315 1.2e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 288 - 337 2.7e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 291 - 350 2.8e-08 Ankyrin repeats (3 copies)
PGG PF13962 400 - 508 1.1e-22 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 536
AasI GACNNNNNNGTC 1 cut(s) 547
Acc16I TGCGCA 1 cut(s) 165
AccBSI CCGCTC 1 cut(s) 1166
AccI GTMKAC 2 cut(s) 333, 1651
AciI CCGC 3 cut(s) 1166, 1266, 1467
AclWI GGATC 5 cut(s) 35, 48, 201, 437, 1068
AcoI YGGCCR 2 cut(s) 574, 1090
AcuI CTGAAG 1 cut(s) 1662
AfaI GTAC 4 cut(s) 5, 13, 1043, 1717
AfiI CCNNNNNNNGG 4 cut(s) 856, 857, 1228, 1417
AflIII ACRYGT 1 cut(s) 256
AgeI ACCGGT 1 cut(s) 818
AgsI TTSAA 3 cut(s) 399, 464, 643
AhlI ACTAGT 1 cut(s) 652
AjnI CCWGG 2 cut(s) 336, 1279
AleI CACNNNNGTG 1 cut(s) 1593
Alw26I GTCTC 2 cut(s) 341, 486
AlwI GGATC 5 cut(s) 35, 48, 201, 437, 1068
Ama87I CYCGRG 1 cut(s) 135
AoxI GGCC 3 cut(s) 574, 676, 1090
AsiGI ACCGGT 1 cut(s) 818
AspA2I CCTAGG 1 cut(s) 706
AspLEI GCGC 2 cut(s) 166, 1534
AspS9I GGNCC 1 cut(s) 1168
AsuC2I CCSGG 1 cut(s) 211
AsuHPI GGTGA 3 cut(s) 488, 722, 777
AsuNHI GCTAGC 2 cut(s) 634, 1321
AvaI CYCGRG 1 cut(s) 135
AvaII GGWCC 1 cut(s) 1168
AvrII CCTAGG 1 cut(s) 706
BaeGI GKGCMC 1 cut(s) 22
BaeI ACNNNNGTAYC 1 cut(s) 28
BalI TGGCCA 2 cut(s) 576, 1092
BamHI GGATCC 1 cut(s) 40
BccI CCATC 7 cut(s) 233, 566, 770, 980, 1049, 1548, 1643
BcgI CGANNNNNNTGC 2 cut(s) 1358, 1392
BciT130I CCWGG 2 cut(s) 338, 1281
BcnI CCSGG 1 cut(s) 211
BcoDI GTCTC 2 cut(s) 341, 486
BcuI ACTAGT 1 cut(s) 652
BfmI CTRYAG 3 cut(s) 1331, 1500, 1648
BlnI CCTAGG 1 cut(s) 706
Bme1390I CCNGG 3 cut(s) 211, 338, 1281
Bme18I GGWCC 1 cut(s) 1168
BmeT110I CYCGRG 1 cut(s) 135
BmgT120I GGNCC 1 cut(s) 1168
BmiI GGNNCC 1 cut(s) 42
BmrFI CCNGG 3 cut(s) 211, 338, 1281
BmrI ACTGGG 1 cut(s) 1577
BmsI GCATC 3 cut(s) 710, 1012, 1288
BmtI GCTAGC 2 cut(s) 638, 1325
BmuI ACTGGG 1 cut(s) 1577
BpmI CTGGAG 1 cut(s) 1302
BpuEI CTTGAG 2 cut(s) 365, 1066
BpuMI CCSGG 1 cut(s) 211
BsaAI YACGTR 1 cut(s) 1552
BsaJI CCNNGG 4 cut(s) 36, 44, 337, 706
BsaWI WCCGGW 1 cut(s) 818
BsaXI ACNNNNNCTCC 5 cut(s) 852, 882, 1563, 1593, 1699
Bsc4I CCNNNNNNNGG 4 cut(s) 856, 857, 1228, 1417
Bse118I RCCGGY 1 cut(s) 818
Bse1I ACTGG 2 cut(s) 909, 1572
Bse3DI GCAATG 2 cut(s) 844, 1197
BseBI CCWGG 2 cut(s) 338, 1281
BseDI CCNNGG 4 cut(s) 36, 44, 337, 706
BseGI GGATG 5 cut(s) 79, 701, 1060, 1582, 1635
BseLI CCNNNNNNNGG 4 cut(s) 856, 857, 1228, 1417
BseMI GCAATG 2 cut(s) 844, 1197
BseMII CTCAG 3 cut(s) 100, 183, 344
BseNI ACTGG 2 cut(s) 909, 1572
BseRI GAGGAG 1 cut(s) 675
BseSI GKGCMC 1 cut(s) 22
BsgI GTGCAG 1 cut(s) 1419
BshFI GGCC 3 cut(s) 576, 678, 1092
BshTI ACCGGT 1 cut(s) 818
BsiHKCI CYCGRG 1 cut(s) 135
BsiSI CCGG 3 cut(s) 210, 679, 819
BslI CCNNNNNNNGG 4 cut(s) 856, 857, 1228, 1417
BsmAI GTCTC 2 cut(s) 341, 486
BsmI GAATGC 4 cut(s) 638, 892, 1268, 1508
BsnI GGCC 3 cut(s) 576, 678, 1092
BsoBI CYCGRG 1 cut(s) 135
Bsp1286I GDGCHC 1 cut(s) 22
Bsp143I GATC 6 cut(s) 40, 206, 442, 667, 1073, 1312
Bsp19I CCATGG 1 cut(s) 36
BspACI CCGC 3 cut(s) 1166, 1266, 1467
BspANI GGCC 3 cut(s) 576, 678, 1092
BspCNI CTCAG 3 cut(s) 99, 184, 345
BspHI TCATGA 1 cut(s) 1560
BspLI GGNNCC 1 cut(s) 42
BspMAI CTGCAG 1 cut(s) 1504
BspOI GCTAGC 2 cut(s) 638, 1325
BspPI GGATC 5 cut(s) 35, 48, 201, 437, 1068
BspQI GCTCTTC 2 cut(s) 897, 1332
BsrBI CCGCTC 1 cut(s) 1166
BsrDI GCAATG 2 cut(s) 844, 1197
BsrFI RCCGGY 1 cut(s) 818
BsrI ACTGG 2 cut(s) 909, 1572
BssAI RCCGGY 1 cut(s) 818
BssECI CCNNGG 4 cut(s) 36, 44, 337, 706
BssMI GATC 6 cut(s) 40, 206, 442, 667, 1073, 1312
BssT1I CCWWGG 3 cut(s) 36, 44, 706
Bst2UI CCWGG 2 cut(s) 338, 1281
Bst4CI ACNGT 7 cut(s) 11, 382, 724, 1231, 1252, 1335, 1673
Bst6I CTCTTC 3 cut(s) 897, 1054, 1332
BstAPI GCANNNNNTGC 2 cut(s) 1016, 1440
BstBAI YACGTR 1 cut(s) 1552
BstC8I GCNNGC 5 cut(s) 591, 636, 1303, 1323, 1405
BstDEI CTNAG 6 cut(s) 86, 98, 192, 235, 353, 1692
BstDSI CCRYGG 1 cut(s) 36
BstEII GGTNACC 1 cut(s) 710
BstF5I GGATG 5 cut(s) 79, 701, 1060, 1582, 1635
BstHHI GCGC 2 cut(s) 166, 1534
BstKTI GATC 6 cut(s) 43, 209, 445, 670, 1076, 1315
BstMAI GTCTC 2 cut(s) 341, 486
BstMBI GATC 6 cut(s) 40, 206, 442, 667, 1073, 1312
BstMWI GCNNNNNNNGC 6 cut(s) 590, 635, 1016, 1187, 1440, 1543
BstNI CCWGG 2 cut(s) 338, 1281
BstNSI RCATGY 2 cut(s) 260, 593
BstPI GGTNACC 1 cut(s) 710
BstSCI CCNGG 3 cut(s) 209, 336, 1279
BstSFI CTRYAG 3 cut(s) 1331, 1500, 1648
BstSLI GKGCMC 1 cut(s) 22
BstX2I RGATCY 3 cut(s) 40, 206, 1073
BstYI RGATCY 3 cut(s) 40, 206, 1073
BsuRI GGCC 3 cut(s) 576, 678, 1092
BtgI CCRYGG 1 cut(s) 36
BtsCI GGATG 5 cut(s) 79, 701, 1060, 1582, 1635
BtsIMutI CAGTG 4 cut(s) 327, 916, 1600, 1669
Cac8I GCNNGC 5 cut(s) 591, 636, 1303, 1323, 1405
CciI TCATGA 1 cut(s) 1560
CfoI GCGC 2 cut(s) 166, 1534
Cfr10I RCCGGY 1 cut(s) 818
Cfr13I GGNCC 1 cut(s) 1168
CseI GACGC 1 cut(s) 1379
Csp6I GTAC 4 cut(s) 4, 12, 1042, 1716
CspAI ACCGGT 1 cut(s) 818
CviAII CATG 8 cut(s) 37, 257, 560, 590, 622, 794, 1150, 1561
CviQI GTAC 4 cut(s) 4, 12, 1042, 1716
DdeI CTNAG 6 cut(s) 86, 98, 192, 235, 353, 1692
DpnI GATC 6 cut(s) 42, 208, 444, 669, 1075, 1314
DpnII GATC 6 cut(s) 40, 206, 442, 667, 1073, 1312
DrdI GACNNNNNNGTC 1 cut(s) 547
DseDI GACNNNNNNGTC 1 cut(s) 547
EaeI YGGCCR 2 cut(s) 574, 1090
Eam1104I CTCTTC 3 cut(s) 897, 1054, 1332
EarI CTCTTC 3 cut(s) 897, 1054, 1332
Eco130I CCWWGG 3 cut(s) 36, 44, 706
Eco47I GGWCC 1 cut(s) 1168
Eco57I CTGAAG 1 cut(s) 1662
Eco88I CYCGRG 1 cut(s) 135
Eco91I GGTNACC 1 cut(s) 710
EcoO65I GGTNACC 1 cut(s) 710
EcoRII CCWGG 2 cut(s) 336, 1279
EcoT14I CCWWGG 3 cut(s) 36, 44, 706
ErhI CCWWGG 3 cut(s) 36, 44, 706
FaeI CATG 8 cut(s) 40, 260, 563, 593, 625, 797, 1153, 1564
FalI AAGNNNNNCTT 2 cut(s) 1012, 1044
FatI CATG 8 cut(s) 36, 256, 559, 589, 621, 793, 1149, 1560
FauNDI CATATG 1 cut(s) 760
FblI GTMKAC 2 cut(s) 333, 1651
FokI GGATG 5 cut(s) 86, 688, 1067, 1589, 1622
FspI TGCGCA 1 cut(s) 165
GlaI GCGC 2 cut(s) 165, 1533
GsuI CTGGAG 1 cut(s) 1302
HaeIII GGCC 3 cut(s) 576, 678, 1092
HapII CCGG 3 cut(s) 210, 679, 819
HgaI GACGC 1 cut(s) 1379
HhaI GCGC 2 cut(s) 166, 1534
Hin1II CATG 8 cut(s) 40, 260, 563, 593, 625, 797, 1153, 1564
Hin6I GCGC 2 cut(s) 164, 1532
HinP1I GCGC 2 cut(s) 164, 1532
HincII GTYRAC 2 cut(s) 292, 871
HindII GTYRAC 2 cut(s) 292, 871
HindIII AAGCTT 1 cut(s) 393
HinfI GANTC 7 cut(s) 349, 479, 526, 682, 854, 1033, 1653
HpaI GTTAAC 1 cut(s) 871
HpaII CCGG 3 cut(s) 210, 679, 819
HphI GGTGA 3 cut(s) 488, 722, 777
Hpy166II GTNNAC 7 cut(s) 292, 334, 785, 871, 914, 1555, 1652
Hpy188I TCNGA 7 cut(s) 89, 173, 354, 571, 979, 1218, 1312
Hpy188III TCNNGA 3 cut(s) 137, 858, 1561
Hpy8I GTNNAC 7 cut(s) 292, 334, 785, 871, 914, 1555, 1652
Hpy99I CGWCG 1 cut(s) 1373
HpyAV CCTTC 3 cut(s) 125, 953, 1609
HpyCH4III ACNGT 7 cut(s) 11, 382, 724, 1231, 1252, 1335, 1673
HpyCH4IV ACGT 1 cut(s) 1551
HpyF10VI GCNNNNNNNGC 6 cut(s) 590, 635, 1016, 1187, 1440, 1543
HpyF3I CTNAG 6 cut(s) 86, 98, 192, 235, 353, 1692
HpySE526I ACGT 1 cut(s) 1551
Hsp92II CATG 8 cut(s) 40, 260, 563, 593, 625, 797, 1153, 1564
HspAI GCGC 2 cut(s) 164, 1532
KspAI GTTAAC 1 cut(s) 871
Kzo9I GATC 6 cut(s) 40, 206, 442, 667, 1073, 1312
LguI GCTCTTC 2 cut(s) 897, 1332
LmnI GCTCC 3 cut(s) 372, 1421, 1505
LweI GCATC 3 cut(s) 710, 1012, 1288
MaeII ACGT 1 cut(s) 1551
MaeIII GTNAC 7 cut(s) 213, 275, 407, 710, 928, 1231, 1252
MalI GATC 6 cut(s) 42, 208, 444, 669, 1075, 1314
MbiI CCGCTC 1 cut(s) 1166
MboI GATC 6 cut(s) 40, 206, 442, 667, 1073, 1312
MboII GAAGA 7 cut(s) 884, 1071, 1135, 1187, 1319, 1357, 1722
MflI RGATCY 3 cut(s) 40, 206, 1073
MhlI GDGCHC 1 cut(s) 22
MlsI TGGCCA 2 cut(s) 576, 1092
MluCI AATT 8 cut(s) 167, 312, 357, 531, 789, 1182, 1392, 1521
MluNI TGGCCA 2 cut(s) 576, 1092
MlyI GAGTC 4 cut(s) 343, 488, 676, 1647
MmeI TCCRAC 1 cut(s) 865
Mox20I TGGCCA 2 cut(s) 576, 1092
MscI TGGCCA 2 cut(s) 576, 1092
MseI TTAA 6 cut(s) 431, 870, 1226, 1428, 1524, 1623
MslI CAYNNNNRTG 3 cut(s) 180, 819, 1593
Msp20I TGGCCA 2 cut(s) 576, 1092
MspA1I CMGCKG 1 cut(s) 1469
MspI CCGG 3 cut(s) 210, 679, 819
MspR9I CCNGG 3 cut(s) 211, 338, 1281
Mva1269I GAATGC 4 cut(s) 638, 892, 1268, 1508
MvaI CCWGG 2 cut(s) 338, 1281
MwoI GCNNNNNNNGC 6 cut(s) 590, 635, 1016, 1187, 1440, 1543
NciI CCSGG 1 cut(s) 211
NcoI CCATGG 1 cut(s) 36
NdeI CATATG 1 cut(s) 760
NdeII GATC 6 cut(s) 40, 206, 442, 667, 1073, 1312
NheI GCTAGC 2 cut(s) 634, 1321
NlaIII CATG 8 cut(s) 40, 260, 563, 593, 625, 797, 1153, 1564
NlaIV GGNNCC 1 cut(s) 42
NmuCI GTSAC 5 cut(s) 213, 275, 407, 710, 928
NsbI TGCGCA 1 cut(s) 165
NspI RCATGY 2 cut(s) 260, 593
OliI CACNNNNGTG 1 cut(s) 1593
PaeI GCATGC 1 cut(s) 593
PaeR7I CTCGAG 1 cut(s) 135
PagI TCATGA 1 cut(s) 1560
PciI ACATGT 1 cut(s) 256
PciSI GCTCTTC 2 cut(s) 897, 1332
PctI GAATGC 4 cut(s) 638, 892, 1268, 1508
PfeI GAWTC 3 cut(s) 526, 854, 1033
PinAI ACCGGT 1 cut(s) 818
PleI GAGTC 4 cut(s) 343, 487, 676, 1647
PpsI GAGTC 4 cut(s) 343, 487, 676, 1647
Ppu21I YACGTR 1 cut(s) 1552
PscI ACATGT 1 cut(s) 256
PsiI TTATAA 1 cut(s) 536
Psp6I CCWGG 2 cut(s) 336, 1279
PspEI GGTNACC 1 cut(s) 710
PspGI CCWGG 2 cut(s) 336, 1279
PspN4I GGNNCC 1 cut(s) 42
PspPI GGNCC 1 cut(s) 1168
PstI CTGCAG 1 cut(s) 1504
PsuI RGATCY 3 cut(s) 40, 206, 1073
RsaI GTAC 4 cut(s) 5, 13, 1043, 1717
RsaNI GTAC 4 cut(s) 4, 12, 1042, 1716
RseI CAYNNNNRTG 3 cut(s) 180, 819, 1593
SapI GCTCTTC 2 cut(s) 897, 1332
SaqAI TTAA 6 cut(s) 431, 870, 1226, 1428, 1524, 1623
Sau3AI GATC 6 cut(s) 40, 206, 442, 667, 1073, 1312
Sau96I GGNCC 1 cut(s) 1168
SchI GAGTC 4 cut(s) 343, 488, 676, 1647
ScrFI CCNGG 3 cut(s) 211, 338, 1281
SduI GDGCHC 1 cut(s) 22
SfaNI GCATC 3 cut(s) 710, 1012, 1288
SfcI CTRYAG 3 cut(s) 1331, 1500, 1648
Sfr274I CTCGAG 1 cut(s) 135
SinI GGWCC 1 cut(s) 1168
SlaI CTCGAG 1 cut(s) 135
SmiMI CAYNNNNRTG 3 cut(s) 180, 819, 1593
SmlI CTYRAG 3 cut(s) 135, 344, 1045
SmoI CTYRAG 3 cut(s) 135, 344, 1045
SpeI ACTAGT 1 cut(s) 652
SphI GCATGC 1 cut(s) 593
Sse9I AATT 8 cut(s) 167, 312, 357, 531, 789, 1182, 1392, 1521
SsiI CCGC 3 cut(s) 1166, 1266, 1467
SspI AATATT 1 cut(s) 1138
StyD4I CCNGG 3 cut(s) 209, 336, 1279
StyI CCWWGG 3 cut(s) 36, 44, 706
TaaI ACNGT 7 cut(s) 11, 382, 724, 1231, 1252, 1335, 1673
TaiI ACGT 1 cut(s) 1554
TaqI TCGA 7 cut(s) 136, 423, 670, 1315, 1337, 1368, 1496
TasI AATT 8 cut(s) 167, 312, 357, 531, 789, 1182, 1392, 1521
TatI WGTACW 2 cut(s) 3, 1715
TauI GCSGC 2 cut(s) 1268, 1469
TfiI GAWTC 3 cut(s) 526, 854, 1033
Tru1I TTAA 6 cut(s) 431, 870, 1226, 1428, 1524, 1623
Tru9I TTAA 6 cut(s) 431, 870, 1226, 1428, 1524, 1623
TscAI CASTG 4 cut(s) 327, 916, 1600, 1676
TseFI GTSAC 5 cut(s) 213, 275, 407, 710, 928
Tsp45I GTSAC 5 cut(s) 213, 275, 407, 710, 928
TspDTI ATGAA 6 cut(s) 306, 782, 884, 1136, 1348, 1604
TspGWI ACGGA 1 cut(s) 537
TspRI CASTG 4 cut(s) 327, 916, 1600, 1676
VpaK11BI GGWCC 1 cut(s) 1168
XceI RCATGY 2 cut(s) 260, 593
XhoI CTCGAG 1 cut(s) 135
XmaJI CCTAGG 1 cut(s) 706
XmiI GTMKAC 2 cut(s) 333, 1651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.