Rroxscaffold_1G00043420

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002418
Physical Location & Seq
Forward (+)
61913249 .. 61918520
5272 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00043420.1

Sequence Viewer

Length: 1329 bp
ATGGAAGCTCTGCTAGCATTCAAGCAAGAACTAGTCAAAGAAGCTGATCGACAAGGCCGGACTCCTCTCTACTATGCAGCATCCCTAGGTGAACACAGAACAGTTCAGCGATTACTAGAACTTGATACTTCTACTGCATATGTTTTGGATAAAGATGGTCGTTCACCAATTCATGTTGCAGCTAGGAATGGTCATACCGGTGTGATTAGAGAGATTATCCAACATTGCCCAGATTCGGGAGAACTTATTGACCCTTATGGACAGAATGCTCTTCATATTGCTATTTCCAGTGGACAAGCTAATGTTGTCACATATATATTAGAAACACCAGAACTAGAAGGGTTGATAAATCAGTCTGATATTAATGGAAACACGCCTTTGCATCTAGCCGCGATTGCAAGAAAAACTTGGATTCTTTGGTACTTGATGTGGGATGGAAGAGTAAATCAAAGATCCAAGGACAAATATGGCCAAACAGCATTTGACAATGATAGGTCAATGAAGAAATCTAGTATTACATCTCCCATGATTTCAGCAAGAGCGGACCACGAAGAAGCAATTTCAGCGATGCAAACTTACAAGAAAATGGGTCAGACCCTTTTAACGGTTACAACACTCATTACAACTGTAACATTTACAGCCGCATTCACAATGCCTGGAGGCTACAACAATGATGCAGGCTCATCAGATCGAGGGCTAGCTCTTCTACAGTCGAGCAAATATCTAAAACATTTCATAATCTTCGACGCTGTTGCTATGACTTGCTCAATTAGTGCAGCTTGCCTCCTATTTTGGGGAGCTGTTAAAAGCAAGGGGTGCTATCTTTACTATTTCATAAGTGCCGCTGCTCTTACATATATTGCTCTACTATCGACTGCAGGAGCATTCACAACAGGAATTAAAGCTGTGCTGCCCCATCAGCAATACGTGGACATCATGATACTGGGAGTGGATGTTGCTTTTCACATCAGTACCTTCTTGTTTCTTTTTCATTTGGTTAAAATGTTTGCCATCCCTGAAGCCTGTAGACTCTTGAAAGCACTTTGCTCGAAGAGCACTACTACCGAAGCCGCAAGTCATAATATAATGATGGAAGAACATACTGGAGCTTTTACCGTTGGAACTGATATGGATTCTAGCATGGGAGCTAGTGTTGCCGCATCCAGTTCAAGCATTCCGATTCGACTCTTTGCTAGTGCTTTCTTCTTTGCAACACTTGATTCTTGTGTTACTCAAGATGTACCGCTGAATTGGGAGATCGATGAAGCATTGAAGCAAACCTGCAAGCTGCAGCTCTTTTTATTTAGGTCTGTAACTACTAAGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

442

Amino Acids

48.61

Weight (kDa)

6.5

Isoelectric Point (pI)

33.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_4 PF13637 23 - 69 4.6e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 48 - 108 1.4e-11 Ankyrin repeats (3 copies)
Ank_5 PF13857 81 - 130 3.1e-06 Ankyrin repeats (many copies)
PGG PF13962 192 - 301 7.7e-24 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1289
AccBSI CCGCTC 1 cut(s) 542
AccI GTMKAC 1 cut(s) 1027
AccII CGCG 1 cut(s) 392
AciI CCGC 7 cut(s) 390, 542, 642, 843, 1071, 1158, 1244
AclWI GGATC 1 cut(s) 447
AcoI YGGCCR 1 cut(s) 469
AcuI CTGAAG 1 cut(s) 1038
AfaI GTAC 3 cut(s) 422, 973, 1242
AfiI CCNNNNNNNGG 4 cut(s) 235, 236, 604, 793
AgeI ACCGGT 1 cut(s) 197
AgsI TTSAA 4 cut(s) 22, 1036, 1170, 1273
AhlI ACTAGT 1 cut(s) 31
AjnI CCWGG 1 cut(s) 655
Alw21I GWGCWC 1 cut(s) 1058
AlwI GGATC 1 cut(s) 447
AoxI GGCC 2 cut(s) 55, 469
ApeKI GCWGC 7 cut(s) 77, 179, 776, 845, 910, 1288, 1291
AseI ATTAAT 1 cut(s) 363
AsiGI ACCGGT 1 cut(s) 197
AspA2I CCTAGG 1 cut(s) 85
AspS9I GGNCC 1 cut(s) 544
AsuHPI GGTGA 2 cut(s) 101, 156
AsuNHI GCTAGC 2 cut(s) 13, 697
AvaII GGWCC 1 cut(s) 544
AvrII CCTAGG 1 cut(s) 85
BaeI ACNNNNGTAYC 2 cut(s) 955, 988
BalI TGGCCA 1 cut(s) 471
Bbv12I GWGCWC 1 cut(s) 1058
BbvI GCAGC 7 cut(s) 89, 191, 788, 832, 897, 1275, 1303
BccI CCATC 5 cut(s) 149, 428, 924, 1019, 1084
BcgI CGANNNNNNTGC 4 cut(s) 734, 768, 1029, 1063
BciT130I CCWGG 1 cut(s) 657
BcuI ACTAGT 1 cut(s) 31
BfmI CTRYAG 4 cut(s) 707, 876, 1024, 1289
BfuAI ACCTGC 1 cut(s) 1289
BlnI CCTAGG 1 cut(s) 85
Bme1390I CCNGG 1 cut(s) 657
Bme18I GGWCC 1 cut(s) 544
BmgT120I GGNCC 1 cut(s) 544
BmrFI CCNGG 1 cut(s) 657
BmrI ACTGGG 1 cut(s) 953
BmsI GCATC 5 cut(s) 89, 391, 558, 664, 1169
BmtI GCTAGC 2 cut(s) 17, 701
BmuI ACTGGG 1 cut(s) 953
BpmI CTGGAG 2 cut(s) 678, 1125
BpuEI CTTGAG 1 cut(s) 1218
Bsa29I ATCGAT 1 cut(s) 1260
BsaAI YACGTR 1 cut(s) 928
BsaJI CCNNGG 2 cut(s) 85, 456
BsaWI WCCGGW 1 cut(s) 197
BsaXI ACNNNNNCTCC 4 cut(s) 939, 969, 1137, 1167
Bsc4I CCNNNNNNNGG 4 cut(s) 235, 236, 604, 793
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 4 cut(s) 288, 948, 1108, 1164
Bse3DI GCAATG 1 cut(s) 223
BseBI CCWGG 1 cut(s) 657
BseCI ATCGAT 1 cut(s) 1260
BseDI CCNNGG 2 cut(s) 85, 456
BseGI GGATG 5 cut(s) 80, 439, 958, 1011, 1160
BseLI CCNNNNNNNGG 4 cut(s) 235, 236, 604, 793
BseMI GCAATG 1 cut(s) 223
BseNI ACTGG 4 cut(s) 288, 948, 1108, 1164
BseRI GAGGAG 1 cut(s) 54
BseXI GCAGC 7 cut(s) 89, 191, 788, 832, 897, 1275, 1303
BsgI GTGCAG 1 cut(s) 795
Bsh1236I CGCG 1 cut(s) 392
BshFI GGCC 2 cut(s) 57, 471
BshTI ACCGGT 1 cut(s) 197
BshVI ATCGAT 1 cut(s) 1260
BsiHKAI GWGCWC 1 cut(s) 1058
BsiSI CCGG 2 cut(s) 58, 198
BslI CCNNNNNNNGG 4 cut(s) 235, 236, 604, 793
BsmI GAATGC 5 cut(s) 17, 271, 644, 884, 1173
BsnI GGCC 2 cut(s) 57, 471
Bsp1286I GDGCHC 1 cut(s) 1058
Bsp143I GATC 4 cut(s) 46, 452, 688, 1257
BspACI CCGC 7 cut(s) 390, 542, 642, 843, 1071, 1158, 1244
BspANI GGCC 2 cut(s) 57, 471
BspDI ATCGAT 1 cut(s) 1260
BspFNI CGCG 1 cut(s) 392
BspHI TCATGA 1 cut(s) 936
BspMAI CTGCAG 2 cut(s) 880, 1293
BspMI ACCTGC 1 cut(s) 1289
BspOI GCTAGC 2 cut(s) 17, 701
BspPI GGATC 1 cut(s) 447
BspQI GCTCTTC 3 cut(s) 276, 708, 1046
BsrBI CCGCTC 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 223
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 4 cut(s) 288, 948, 1108, 1164
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 2 cut(s) 85, 456
BssMI GATC 4 cut(s) 46, 452, 688, 1257
BssT1I CCWWGG 2 cut(s) 85, 456
Bst2UI CCWGG 1 cut(s) 657
Bst4CI ACNGT 5 cut(s) 103, 607, 628, 711, 1117
Bst6I CTCTTC 4 cut(s) 276, 433, 708, 1046
BstAPI GCANNNNNTGC 1 cut(s) 816
BstBAI YACGTR 1 cut(s) 928
BstC8I GCNNGC 5 cut(s) 15, 679, 699, 781, 1286
BstDEI CTNAG 1 cut(s) 1320
BstF5I GGATG 5 cut(s) 80, 439, 958, 1011, 1160
BstFNI CGCG 1 cut(s) 392
BstKTI GATC 4 cut(s) 49, 455, 691, 1260
BstMBI GATC 4 cut(s) 46, 452, 688, 1257
BstMWI GCNNNNNNNGC 7 cut(s) 14, 395, 563, 816, 919, 1053, 1154
BstNI CCWGG 1 cut(s) 657
BstSCI CCNGG 1 cut(s) 655
BstSFI CTRYAG 4 cut(s) 707, 876, 1024, 1289
BstUI CGCG 1 cut(s) 392
BstV1I GCAGC 7 cut(s) 89, 191, 788, 832, 897, 1275, 1303
BstX2I RGATCY 1 cut(s) 452
BstYI RGATCY 1 cut(s) 452
Bsu15I ATCGAT 1 cut(s) 1260
BsuRI GGCC 2 cut(s) 57, 471
BsuTUI ATCGAT 1 cut(s) 1260
BtgZI GCGATG 1 cut(s) 581
BtsCI GGATG 5 cut(s) 80, 439, 958, 1011, 1160
BtsIMutI CAGTG 1 cut(s) 295
BveI ACCTGC 1 cut(s) 1289
Cac8I GCNNGC 5 cut(s) 15, 679, 699, 781, 1286
CciI TCATGA 1 cut(s) 936
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 544
ClaI ATCGAT 1 cut(s) 1260
CseI GACGC 1 cut(s) 755
Csp6I GTAC 3 cut(s) 421, 972, 1241
CspAI ACCGGT 1 cut(s) 197
CviAII CATG 4 cut(s) 173, 526, 937, 1141
CviQI GTAC 3 cut(s) 421, 972, 1241
DdeI CTNAG 1 cut(s) 1320
DpnI GATC 4 cut(s) 48, 454, 690, 1259
DpnII GATC 4 cut(s) 46, 452, 688, 1257
EaeI YGGCCR 1 cut(s) 469
Eam1104I CTCTTC 4 cut(s) 276, 433, 708, 1046
EarI CTCTTC 4 cut(s) 276, 433, 708, 1046
Eco130I CCWWGG 2 cut(s) 85, 456
Eco47I GGWCC 1 cut(s) 544
Eco57I CTGAAG 1 cut(s) 1038
EcoRII CCWGG 1 cut(s) 655
EcoT14I CCWWGG 2 cut(s) 85, 456
ErhI CCWWGG 2 cut(s) 85, 456
FaeI CATG 4 cut(s) 176, 529, 940, 1144
FalI AAGNNNNNCTT 2 cut(s) 391, 423
FatI CATG 4 cut(s) 172, 525, 936, 1140
FauNDI CATATG 1 cut(s) 139
FblI GTMKAC 1 cut(s) 1027
FokI GGATG 5 cut(s) 67, 446, 965, 998, 1147
GsuI CTGGAG 2 cut(s) 678, 1125
HaeIII GGCC 2 cut(s) 57, 471
HapII CCGG 2 cut(s) 58, 198
HgaI GACGC 1 cut(s) 755
Hin1II CATG 4 cut(s) 176, 529, 940, 1144
HinfI GANTC 8 cut(s) 61, 233, 412, 1029, 1133, 1180, 1185, 1220
HpaII CCGG 2 cut(s) 58, 198
HphI GGTGA 2 cut(s) 101, 156
Hpy166II GTNNAC 5 cut(s) 92, 164, 293, 931, 1028
Hpy188I TCNGA 4 cut(s) 358, 594, 688, 1179
Hpy188III TCNNGA 4 cut(s) 237, 937, 1033, 1235
Hpy8I GTNNAC 5 cut(s) 92, 164, 293, 931, 1028
Hpy99I CGWCG 1 cut(s) 749
HpyAV CCTTC 2 cut(s) 332, 985
HpyCH4III ACNGT 5 cut(s) 103, 607, 628, 711, 1117
HpyCH4IV ACGT 1 cut(s) 927
HpyF10VI GCNNNNNNNGC 7 cut(s) 14, 395, 563, 816, 919, 1053, 1154
HpyF3I CTNAG 1 cut(s) 1320
HpySE526I ACGT 1 cut(s) 927
Hsp92II CATG 4 cut(s) 176, 529, 940, 1144
Kzo9I GATC 4 cut(s) 46, 452, 688, 1257
LguI GCTCTTC 3 cut(s) 276, 708, 1046
LmnI GCTCC 4 cut(s) 797, 881, 1106, 1145
Lsp1109I GCAGC 7 cut(s) 89, 191, 788, 832, 897, 1275, 1303
LweI GCATC 5 cut(s) 89, 391, 558, 664, 1169
MaeII ACGT 1 cut(s) 927
MaeIII GTNAC 5 cut(s) 307, 607, 628, 1228, 1312
MalI GATC 4 cut(s) 48, 454, 690, 1259
MbiI CCGCTC 1 cut(s) 542
MboI GATC 4 cut(s) 46, 452, 688, 1257
MboII GAAGA 9 cut(s) 263, 450, 514, 563, 695, 733, 1063, 1106, 1195
MflI RGATCY 1 cut(s) 452
MhlI GDGCHC 1 cut(s) 1058
MlsI TGGCCA 1 cut(s) 471
MluCI AATT 5 cut(s) 168, 558, 768, 897, 1249
MluNI TGGCCA 1 cut(s) 471
MlyI GAGTC 3 cut(s) 55, 1023, 1179
MmeI TCCRAC 2 cut(s) 244, 1099
MnlI CCTC 4 cut(s) 75, 653, 686, 794
Mox20I TGGCCA 1 cut(s) 471
MscI TGGCCA 1 cut(s) 471
MseI TTAA 6 cut(s) 363, 602, 804, 900, 999, 1327
MslI CAYNNNNRTG 1 cut(s) 198
Msp20I TGGCCA 1 cut(s) 471
MspA1I CMGCKG 2 cut(s) 845, 1246
MspI CCGG 2 cut(s) 58, 198
MspR9I CCNGG 1 cut(s) 657
Mva1269I GAATGC 5 cut(s) 17, 271, 644, 884, 1173
MvaI CCWGG 1 cut(s) 657
MvnI CGCG 1 cut(s) 392
MwoI GCNNNNNNNGC 7 cut(s) 14, 395, 563, 816, 919, 1053, 1154
NdeI CATATG 1 cut(s) 139
NdeII GATC 4 cut(s) 46, 452, 688, 1257
NheI GCTAGC 2 cut(s) 13, 697
NlaIII CATG 4 cut(s) 176, 529, 940, 1144
NmuCI GTSAC 1 cut(s) 307
PagI TCATGA 1 cut(s) 936
PciSI GCTCTTC 3 cut(s) 276, 708, 1046
PctI GAATGC 5 cut(s) 17, 271, 644, 884, 1173
PfeI GAWTC 5 cut(s) 233, 412, 1133, 1180, 1220
PinAI ACCGGT 1 cut(s) 197
PleI GAGTC 3 cut(s) 55, 1023, 1179
PpsI GAGTC 3 cut(s) 55, 1023, 1179
Ppu21I YACGTR 1 cut(s) 928
PshBI ATTAAT 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 655
PspGI CCWGG 1 cut(s) 655
PspPI GGNCC 1 cut(s) 544
PstI CTGCAG 2 cut(s) 880, 1293
PsuI RGATCY 1 cut(s) 452
RsaI GTAC 3 cut(s) 422, 973, 1242
RsaNI GTAC 3 cut(s) 421, 972, 1241
RseI CAYNNNNRTG 1 cut(s) 198
SapI GCTCTTC 3 cut(s) 276, 708, 1046
SaqAI TTAA 6 cut(s) 363, 602, 804, 900, 999, 1327
Sau3AI GATC 4 cut(s) 46, 452, 688, 1257
Sau96I GGNCC 1 cut(s) 544
SchI GAGTC 3 cut(s) 55, 1023, 1179
ScrFI CCNGG 1 cut(s) 657
SduI GDGCHC 1 cut(s) 1058
SfaNI GCATC 5 cut(s) 89, 391, 558, 664, 1169
SfcI CTRYAG 4 cut(s) 707, 876, 1024, 1289
SinI GGWCC 1 cut(s) 544
SmiMI CAYNNNNRTG 1 cut(s) 198
SmlI CTYRAG 1 cut(s) 1233
SmoI CTYRAG 1 cut(s) 1233
SpeI ACTAGT 1 cut(s) 31
Sse9I AATT 5 cut(s) 168, 558, 768, 897, 1249
SsiI CCGC 7 cut(s) 390, 542, 642, 843, 1071, 1158, 1244
StyD4I CCNGG 1 cut(s) 655
StyI CCWWGG 2 cut(s) 85, 456
TaaI ACNGT 5 cut(s) 103, 607, 628, 711, 1117
TaiI ACGT 1 cut(s) 930
TaqI TCGA 8 cut(s) 49, 691, 713, 744, 872, 1049, 1183, 1260
TasI AATT 5 cut(s) 168, 558, 768, 897, 1249
TauI GCSGC 5 cut(s) 392, 644, 845, 1073, 1160
TfiI GAWTC 5 cut(s) 233, 412, 1133, 1180, 1220
Tru1I TTAA 6 cut(s) 363, 602, 804, 900, 999, 1327
Tru9I TTAA 6 cut(s) 363, 602, 804, 900, 999, 1327
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 7 cut(s) 77, 179, 776, 845, 910, 1288, 1291
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 7 cut(s) 161, 263, 515, 724, 823, 980, 1278
TspRI CASTG 1 cut(s) 295
VpaK11BI GGWCC 1 cut(s) 544
VspI ATTAAT 1 cut(s) 363
XmaJI CCTAGG 1 cut(s) 85
XmiI GTMKAC 1 cut(s) 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.