pycom12g00680

Exosome component 10-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0004155
Physical Location & Seq
Forward (+)
626624 .. 628347
1724 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 864 bp
ATGAGCCAAGACGCCATGACAGTGAACCAATCTCCGCCGCTGCAGTCGAGGTCCGACGCCCTAGAAAACCTAACCAAAGGCCCACTCTCCTCGTCGATTTCCAAGCTCTCCGGCTCCTCGCGATTCTTGCCTAACAGCAAGGACTTCTATTTCTACCGCAACTTCGACGAATTCAAAGCCCCGATCGAGAAAATCACCAAGGAGGCGCAGACGATGCTCGGCTCGATTGGCTCATCGGCGCCCGTTTTGGGGAAACCGATGGCTTTCCCCCAAGACTTGGACGACGGGTACGATTGGCTCGTTAATGTGAACGACGAGGTGCTGGAGAGGTTTGACTCGTCGGTGGATGAGTTCAAGAGGGTGAGGAAGGAGGCGGAGGAGGCCAAGCGGCCGACGAGTGCGGCATTGGACACAGACGATGGGTTTCAGTTGGTGTGTGGGAAGAAGAAGAAAAAGGGGTCCACTGGGTTGGCTTCGGCCAGTGGGAACGATGATTCGAGTCAGGTTTCCACCGCCGTGAAGGTGGCTGCCAAGGACAAGAAAACTGTGGCGGCGACGAAGCCCAAGGTTCCGTTTCATATACCAAGCATTCGGAGGTCTCAGGAGGAGTTTAACATTTTGGTTAACAATGCAAACCAGCCGTTTGAGCATGTTTTGTTGCAGAGAAGTGAGGATGGTCAGCGGTTCCTTCATCCCCTAGAGGAGCTTTCTGTTCTGGATTTTGTTGACAACAACGTCGGGGATGTTGAGTCTGTAAAACCGCCTTCATTAAAGTCTACGCCATTCAAGCTTGTGGAGGAGGTCAAAGATCTGGAAGAGCTAGCAGCTAAGCTCCGTGCTGTTAATGAATTTGCGCTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0000178 GO:0000460 GO:0000785 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006403 GO:0006725 GO:0006807 GO:0007549 GO:0008150 GO:0008152 GO:0008298 GO:0008334 GO:0008408 GO:0009048 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010639 GO:0016070 GO:0016071 GO:0016072 GO:0016075 GO:0016458 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0031047 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032210 GO:0032211 GO:0032259 GO:0032268 GO:0032270 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035327 GO:0040029 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0045934 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051246 GO:0051247 GO:0051641 GO:0060255 GO:0061085 GO:0061087 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071025 GO:0071027 GO:0071028 GO:0071029 GO:0071030 GO:0071033 GO:0071034 GO:0071035 GO:0071043 GO:0071044 GO:0071046 GO:0071048 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1902275 GO:1902464 GO:1902466 GO:1902494 GO:1904356 GO:1904357 GO:1904872 GO:1905269 GO:1905354 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DNA_pol_A_exo1 HRDC PMC2NT

Protein Analysis

288

Amino Acids

31.67

Weight (kDa)

5.34

Isoelectric Point (pI)

55.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMC2NT PF08066 39 - 119 1.3e-08 PMC2NT (NUC016) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 734
AccB1I GGYRCC 1 cut(s) 238
AccB7I CCANNNNNTGG 1 cut(s) 277
AccI GTMKAC 1 cut(s) 776
AccII CGCG 1 cut(s) 121
AcoI YGGCCR 2 cut(s) 389, 477
AcsI RAATTY 2 cut(s) 170, 848
AcyI GRCGYC 3 cut(s) 12, 57, 239
AfaI GTAC 1 cut(s) 290
AfiI CCNNNNNNNGG 3 cut(s) 248, 249, 277
AgsI TTSAA 3 cut(s) 175, 355, 787
AleI CACNNNNGTG 1 cut(s) 515
AluBI AGCT 6 cut(s) 106, 706, 790, 820, 827, 832
AluI AGCT 6 cut(s) 106, 706, 790, 820, 827, 832
Alw26I GTCTC 1 cut(s) 603
AoxI GGCC 4 cut(s) 79, 381, 389, 477
ApeKI GCWGC 3 cut(s) 40, 527, 824
ApoI RAATTY 2 cut(s) 170, 848
AspLEI GCGC 3 cut(s) 208, 241, 856
AspS9I GGNCC 3 cut(s) 51, 80, 459
AsuHPI GGTGA 2 cut(s) 187, 373
AsuNHI GCTAGC 1 cut(s) 820
AvaII GGWCC 2 cut(s) 51, 459
BanI GGYRCC 1 cut(s) 238
BbvI GCAGC 3 cut(s) 27, 514, 836
BccI CCATC 3 cut(s) 253, 413, 668
BceAI ACGGC 2 cut(s) 500, 625
BcoDI GTCTC 1 cut(s) 603
BfaI CTAG 3 cut(s) 62, 698, 821
BfmI CTRYAG 1 cut(s) 41
BfoI RGCGCY 1 cut(s) 242
BglII AGATCT 1 cut(s) 808
BisI GCNGC 7 cut(s) 38, 41, 389, 402, 528, 552, 825
BlpI GCTNAGC 1 cut(s) 828
BlsI GCNGC 7 cut(s) 39, 42, 390, 403, 529, 553, 826
Bme18I GGWCC 2 cut(s) 51, 459
BmgT120I GGNCC 3 cut(s) 51, 80, 459
BmiI GGNNCC 5 cut(s) 115, 240, 460, 570, 686
BmrI ACTGGG 1 cut(s) 474
BmsI GCATC 1 cut(s) 204
BmtI GCTAGC 1 cut(s) 824
BmuI ACTGGG 1 cut(s) 474
BpmI CTGGAG 1 cut(s) 344
Bpu1102I GCTNAGC 1 cut(s) 828
BsaHI GRCGYC 3 cut(s) 12, 57, 239
BsaI GGTCTC 1 cut(s) 603
BsaJI CCNNGG 3 cut(s) 198, 531, 564
Bsc4I CCNNNNNNNGG 3 cut(s) 248, 249, 277
Bse1I ACTGG 2 cut(s) 469, 480
BseDI CCNNGG 3 cut(s) 198, 531, 564
BseGI GGATG 4 cut(s) 352, 679, 691, 748
BseLI CCNNNNNNNGG 3 cut(s) 248, 249, 277
BseMII CTCAG 1 cut(s) 614
BseNI ACTGG 2 cut(s) 469, 480
BseRI GAGGAG 6 cut(s) 79, 106, 392, 620, 716, 812
BseX3I CGGCCG 1 cut(s) 389
BseXI GCAGC 3 cut(s) 27, 514, 836
Bsh1236I CGCG 1 cut(s) 121
Bsh1285I CGRYCG 2 cut(s) 186, 392
BshFI GGCC 4 cut(s) 81, 383, 391, 479
BshNI GGYRCC 1 cut(s) 238
BsiEI CGRYCG 2 cut(s) 186, 392
BsiSI CCGG 1 cut(s) 111
BslI CCNNNNNNNGG 3 cut(s) 248, 249, 277
BsmAI GTCTC 1 cut(s) 603
BsmI GAATGC 1 cut(s) 588
BsnI GGCC 4 cut(s) 81, 383, 391, 479
Bso31I GGTCTC 1 cut(s) 603
Bsp143I GATC 2 cut(s) 183, 808
Bsp1720I GCTNAGC 1 cut(s) 828
Bsp68I TCGCGA 1 cut(s) 121
BspANI GGCC 4 cut(s) 81, 383, 391, 479
BspCNI CTCAG 1 cut(s) 613
BspFNI CGCG 1 cut(s) 121
BspLI GGNNCC 5 cut(s) 115, 240, 460, 570, 686
BspMAI CTGCAG 1 cut(s) 45
BspOI GCTAGC 1 cut(s) 824
BspQI GCTCTTC 1 cut(s) 810
BspT107I GGYRCC 1 cut(s) 238
BspTNI GGTCTC 1 cut(s) 603
BsrI ACTGG 2 cut(s) 469, 480
BssECI CCNNGG 3 cut(s) 198, 531, 564
BssMI GATC 2 cut(s) 183, 808
BssNI GRCGYC 3 cut(s) 12, 57, 239
BssT1I CCWWGG 3 cut(s) 198, 531, 564
Bst4CI ACNGT 2 cut(s) 22, 547
Bst6I CTCTTC 1 cut(s) 810
BstACI GRCGYC 3 cut(s) 12, 57, 239
BstAPI GCANNNNNTGC 1 cut(s) 214
BstC8I GCNNGC 1 cut(s) 822
BstDEI CTNAG 2 cut(s) 600, 828
BstF5I GGATG 4 cut(s) 352, 679, 691, 748
BstFNI CGCG 1 cut(s) 121
BstH2I RGCGCY 1 cut(s) 242
BstHHI GCGC 3 cut(s) 208, 241, 856
BstKTI GATC 2 cut(s) 186, 811
BstMAI GTCTC 1 cut(s) 603
BstMBI GATC 2 cut(s) 183, 808
BstMCI CGRYCG 2 cut(s) 186, 392
BstMWI GCNNNNNNNGC 6 cut(s) 127, 214, 228, 380, 646, 787
BstNSI RCATGY 1 cut(s) 653
BstSFI CTRYAG 1 cut(s) 41
BstUI CGCG 1 cut(s) 121
BstV1I GCAGC 3 cut(s) 27, 514, 836
BstX2I RGATCY 1 cut(s) 808
BstXI CCANNNNNNTGG 1 cut(s) 469
BstYI RGATCY 1 cut(s) 808
BstZI CGGCCG 1 cut(s) 389
BsuRI GGCC 4 cut(s) 81, 383, 391, 479
BtsCI GGATG 4 cut(s) 352, 679, 691, 748
BtsIMutI CAGTG 3 cut(s) 27, 462, 487
BtuMI TCGCGA 1 cut(s) 121
Cac8I GCNNGC 1 cut(s) 822
CfoI GCGC 3 cut(s) 208, 241, 856
Cfr13I GGNCC 3 cut(s) 51, 80, 459
CseI GACGC 2 cut(s) 20, 65
Csp6I GTAC 1 cut(s) 289
CviAII CATG 2 cut(s) 16, 650
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 2 cut(s) 600, 828
DinI GGCGCC 1 cut(s) 240
DpnI GATC 2 cut(s) 185, 810
DpnII GATC 2 cut(s) 183, 808
DrdI GACNNNNNNGTC 1 cut(s) 734
DseDI GACNNNNNNGTC 1 cut(s) 734
EaeI YGGCCR 2 cut(s) 389, 477
EagI CGGCCG 1 cut(s) 389
Eam1104I CTCTTC 1 cut(s) 810
EarI CTCTTC 1 cut(s) 810
EciI GGCGGA 2 cut(s) 24, 389
EclXI CGGCCG 1 cut(s) 389
Eco130I CCWWGG 3 cut(s) 198, 531, 564
Eco31I GGTCTC 1 cut(s) 603
Eco47I GGWCC 2 cut(s) 51, 459
Eco52I CGGCCG 1 cut(s) 389
EcoRI GAATTC 1 cut(s) 170
EcoT14I CCWWGG 3 cut(s) 198, 531, 564
EgeI GGCGCC 1 cut(s) 240
EheI GGCGCC 1 cut(s) 240
ErhI CCWWGG 3 cut(s) 198, 531, 564
FaeI CATG 2 cut(s) 19, 653
FaiI YATR 4 cut(s) 17, 579, 581, 651
FatI CATG 2 cut(s) 15, 649
FblI GTMKAC 1 cut(s) 776
Fnu4HI GCNGC 7 cut(s) 38, 41, 389, 402, 528, 552, 825
FokI GGATG 4 cut(s) 359, 678, 686, 755
Fsp4HI GCNGC 7 cut(s) 38, 41, 389, 402, 528, 552, 825
FspBI CTAG 3 cut(s) 62, 698, 821
GlaI GCGC 3 cut(s) 207, 240, 855
GluI GCNGC 7 cut(s) 38, 41, 389, 402, 528, 552, 825
GsuI CTGGAG 1 cut(s) 344
HaeII RGCGCY 1 cut(s) 242
HaeIII GGCC 4 cut(s) 81, 383, 391, 479
HapII CCGG 1 cut(s) 111
HgaI GACGC 2 cut(s) 20, 65
HhaI GCGC 3 cut(s) 208, 241, 856
Hin1I GRCGYC 3 cut(s) 12, 57, 239
Hin1II CATG 2 cut(s) 19, 653
Hin6I GCGC 3 cut(s) 206, 239, 854
HinP1I GCGC 3 cut(s) 206, 239, 854
HincII GTYRAC 2 cut(s) 625, 727
HindII GTYRAC 2 cut(s) 625, 727
HindIII AAGCTT 1 cut(s) 788
HinfI GANTC 5 cut(s) 123, 335, 494, 499, 749
HpaI GTTAAC 1 cut(s) 625
HpaII CCGG 1 cut(s) 111
HphI GGTGA 2 cut(s) 187, 373
Hpy166II GTNNAC 6 cut(s) 25, 310, 462, 625, 727, 777
Hpy188I TCNGA 2 cut(s) 55, 594
Hpy188III TCNNGA 6 cut(s) 120, 187, 355, 602, 716, 812
Hpy8I GTNNAC 6 cut(s) 25, 310, 462, 625, 727, 777
Hpy99I CGWCG 9 cut(s) 59, 97, 170, 287, 317, 343, 397, 559, 740
HpyAV CCTTC 4 cut(s) 361, 514, 698, 774
HpyCH4III ACNGT 2 cut(s) 22, 547
HpyCH4IV ACGT 1 cut(s) 735
HpyCH4V TGCA 3 cut(s) 43, 632, 661
HpyF10VI GCNNNNNNNGC 6 cut(s) 127, 214, 228, 380, 646, 787
HpyF3I CTNAG 2 cut(s) 600, 828
HpySE526I ACGT 1 cut(s) 735
Hsp92I GRCGYC 3 cut(s) 12, 57, 239
Hsp92II CATG 2 cut(s) 19, 653
HspAI GCGC 3 cut(s) 206, 239, 854
KasI GGCGCC 1 cut(s) 238
KspAI GTTAAC 1 cut(s) 625
Kzo9I GATC 2 cut(s) 183, 808
LguI GCTCTTC 1 cut(s) 810
LmnI GCTCC 3 cut(s) 119, 703, 837
LpnPI CCDG 9 cut(s) 124, 308, 450, 488, 493, 587, 650, 701, 797
Lsp1109I GCAGC 3 cut(s) 27, 514, 836
LweI GCATC 1 cut(s) 204
MaeI CTAG 3 cut(s) 62, 698, 821
MaeII ACGT 1 cut(s) 735
MalI GATC 2 cut(s) 185, 810
MboI GATC 2 cut(s) 183, 808
MboII GAAGA 4 cut(s) 454, 457, 460, 827
MflI RGATCY 1 cut(s) 808
MluCI AATT 2 cut(s) 170, 848
Mly113I GGCGCC 1 cut(s) 239
MlyI GAGTC 3 cut(s) 329, 508, 758
MmeI TCCRAC 1 cut(s) 78
MseI TTAA 5 cut(s) 303, 612, 624, 770, 843
MslI CAYNNNNRTG 2 cut(s) 20, 515
MspA1I CMGCKG 2 cut(s) 40, 682
MspI CCGG 1 cut(s) 111
Mva1269I GAATGC 1 cut(s) 588
MvnI CGCG 1 cut(s) 121
MwoI GCNNNNNNNGC 6 cut(s) 127, 214, 228, 380, 646, 787
NarI GGCGCC 1 cut(s) 239
NdeII GATC 2 cut(s) 183, 808
NheI GCTAGC 1 cut(s) 820
NlaIII CATG 2 cut(s) 19, 653
NlaIV GGNNCC 5 cut(s) 115, 240, 460, 570, 686
NmeAIII GCCGAG 1 cut(s) 198
NruI TCGCGA 1 cut(s) 121
NspI RCATGY 1 cut(s) 653
OliI CACNNNNGTG 1 cut(s) 515
PciSI GCTCTTC 1 cut(s) 810
PcsI WCGNNNNNNNCGW 2 cut(s) 288, 297
PctI GAATGC 1 cut(s) 588
PfeI GAWTC 2 cut(s) 123, 494
PflMI CCANNNNNTGG 1 cut(s) 277
PkrI GCNGC 7 cut(s) 39, 42, 390, 403, 529, 553, 826
Ple19I CGATCG 1 cut(s) 186
PleI GAGTC 3 cut(s) 329, 507, 757
PluTI GGCGCC 1 cut(s) 242
PpsI GAGTC 3 cut(s) 329, 507, 757
PspN4I GGNNCC 5 cut(s) 115, 240, 460, 570, 686
PspPI GGNCC 3 cut(s) 51, 80, 459
PstI CTGCAG 1 cut(s) 45
PsuI RGATCY 1 cut(s) 808
PvuI CGATCG 1 cut(s) 186
RruI TCGCGA 1 cut(s) 121
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
RseI CAYNNNNRTG 2 cut(s) 20, 515
SapI GCTCTTC 1 cut(s) 810
SaqAI TTAA 5 cut(s) 303, 612, 624, 770, 843
SatI GCNGC 7 cut(s) 38, 41, 389, 402, 528, 552, 825
Sau3AI GATC 2 cut(s) 183, 808
Sau96I GGNCC 3 cut(s) 51, 80, 459
SchI GAGTC 3 cut(s) 329, 508, 758
SfaNI GCATC 1 cut(s) 204
SfcI CTRYAG 1 cut(s) 41
SfoI GGCGCC 1 cut(s) 240
SinI GGWCC 2 cut(s) 51, 459
SmiMI CAYNNNNRTG 2 cut(s) 20, 515
Sse9I AATT 2 cut(s) 170, 848
SspDI GGCGCC 1 cut(s) 238
SspMI CTAG 3 cut(s) 62, 698, 821
StyI CCWWGG 3 cut(s) 198, 531, 564
TaaI ACNGT 2 cut(s) 22, 547
TaiI ACGT 1 cut(s) 738
TaqI TCGA 6 cut(s) 47, 95, 165, 186, 224, 497
TasI AATT 2 cut(s) 170, 848
TauI GCSGC 4 cut(s) 40, 391, 404, 554
TfiI GAWTC 2 cut(s) 123, 494
Tru1I TTAA 5 cut(s) 303, 612, 624, 770, 843
Tru9I TTAA 5 cut(s) 303, 612, 624, 770, 843
TscAI CASTG 3 cut(s) 27, 469, 487
TseI GCWGC 3 cut(s) 40, 527, 824
TspDTI ATGAA 4 cut(s) 566, 680, 756, 861
TspGWI ACGGA 2 cut(s) 561, 824
TspRI CASTG 3 cut(s) 27, 469, 487
Van91I CCANNNNNTGG 1 cut(s) 277
VpaK11BI GGWCC 2 cut(s) 51, 459
XapI RAATTY 2 cut(s) 170, 848
XceI RCATGY 1 cut(s) 653
XmiI GTMKAC 1 cut(s) 776
XspI CTAG 3 cut(s) 62, 698, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.