pycom12g00750

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0004377
Physical Location & Seq
Forward (+)
665431 .. 669007
3577 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1098 bp
ATGGGCTCTTCTCTCTCCTCTGCCATTCTCCTTCTTCTCTTTGCTTTCTGTCTCTCTCTTCCGACCCTCTCACCTGCCACCGAACTCAAAGGTGTTGATATTGAAAACCCAGTATTAGAAGTCATCCCGGCACCTCTTTCTGAGCACTCAGCTGTGCCTGGTTCTAAAGATGTTTTATTTTGCGTACGGGCACGAGTTTCTGGTATATCCAGGTTAAAACTTGGGAGTTATGCAAGTTCACTTAAGATCACCTTGTCTGCATCTCTAGGAATCCTGGAGAGGTTGCTTAGCAAAATTCAAGTCTGTTTTCATAGGAATAATACGCTTGGATTATGTCAGTGCGAAGAGGATGATTGGAAAAATATTCAGAAAGGGTTATGGAGCTCTGTCATATCGCCTTATGGTGAAAGATATGTCGATGTCAAGTTCATTGGCAAAATACCTGGCCCTGTCACCATAACTGTTGAAGAAGATTTTCAGAGATCTCGCCTTTTGTGTCTGGCACTGGGATTTACTTTACTGTTGTTGGCACCAATTGTCAGCAATTGGGTACCTTTTTATTATAGTAGTTCAATGGTTATAGGAGTTTTTCTGGTCATTATAATCATTCTCTTCCAGGGAATGAAGCTGTTGCTAACTGAGGGGAAAAATGTCCTCTATCTTACCATATGTGGATCAGTGCTTGGAGCAGGATCTTTCCTTGTTCATGAGTTTTCACTGATGGTAAATTCAATTCTTTTGAGTTTTGACCCTTTTGGACTAAGTGAGGAGATTCATAACCCAGTCTCTATATTTTTACTAGTGGGAATTATCCTCCCGGGAGCTGCATTAGGGTACTGGATTGTGAGGAAGTTTGTTGTCTCAAAAGATGGGACAGTAGATGTTGGTATAGCACAATTTGTCAAGTGGGCTATGCGTATCATTGGAACAACCTCAATCTTGCAGAGCACTCTCGATACTCCTTTAGCAATGGGCGCTTTGGATTCTTACTCGATCATCAACAAGTTGATTACTTGTCTGAAATGGCATCTCAAATCCAAATTGCAGCAGAAAAGCTGGAAGACAAGCAACAGACAAGCTTCAGAAATGAAGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

366

Amino Acids

40.07

Weight (kDa)

8.82

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 152 - 359 3.7e-45 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 602
AarI CACCTGC 1 cut(s) 82
Acc36I ACCTGC 1 cut(s) 82
Acc65I GGTACC 1 cut(s) 550
AccB1I GGYRCC 3 cut(s) 130, 529, 550
AclWI GGATC 2 cut(s) 682, 700
AcsI RAATTY 2 cut(s) 294, 727
AcuI CTGAAG 1 cut(s) 1065
AfaI GTAC 3 cut(s) 186, 552, 836
AflII CTTAAG 1 cut(s) 242
AgsI TTSAA 5 cut(s) 104, 299, 467, 573, 732
AhlI ACTAGT 1 cut(s) 799
AjnI CCWGG 5 cut(s) 157, 209, 273, 442, 615
AluBI AGCT 6 cut(s) 152, 384, 628, 824, 1056, 1079
AluI AGCT 6 cut(s) 152, 384, 628, 824, 1056, 1079
Alw21I GWGCWC 3 cut(s) 147, 386, 950
Alw26I GTCTC 3 cut(s) 56, 790, 865
AlwI GGATC 2 cut(s) 682, 700
Ama87I CYCGRG 1 cut(s) 817
AoxI GGCC 1 cut(s) 445
ApeKI GCWGC 2 cut(s) 824, 1045
ApoI RAATTY 2 cut(s) 294, 727
ArsI GACNNNNNNTTYG 2 cut(s) 285, 317
Asp700I GAANNNNTTC 1 cut(s) 474
Asp718I GGTACC 1 cut(s) 550
AspLEI GCGC 1 cut(s) 977
AspS9I GGNCC 1 cut(s) 446
AsuC2I CCSGG 3 cut(s) 128, 818, 819
AsuHPI GGTGA 4 cut(s) 63, 241, 416, 445
AvaI CYCGRG 1 cut(s) 817
BaeGI GKGCMC 1 cut(s) 193
BanI GGYRCC 3 cut(s) 130, 529, 550
BanII GRGCYC 2 cut(s) 8, 386
BauI CACGAG 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 1067
Bbv12I GWGCWC 3 cut(s) 147, 386, 950
BbvI GCAGC 2 cut(s) 811, 1057
BccI CCATC 2 cut(s) 715, 863
BciT130I CCWGG 5 cut(s) 159, 211, 275, 444, 617
BcnI CCSGG 3 cut(s) 128, 818, 819
BcoDI GTCTC 3 cut(s) 56, 790, 865
BcuI ACTAGT 1 cut(s) 799
BfaI CTAG 2 cut(s) 266, 800
BfoI RGCGCY 1 cut(s) 978
BfrI CTTAAG 1 cut(s) 242
BfuAI ACCTGC 1 cut(s) 82
BglII AGATCT 1 cut(s) 482
BisI GCNGC 2 cut(s) 825, 1046
BlpI GCTNAGC 1 cut(s) 287
BlsI GCNGC 2 cut(s) 826, 1047
Bme1390I CCNGG 8 cut(s) 128, 159, 211, 275, 444, 617, 818, 819
BmeT110I CYCGRG 1 cut(s) 817
BmgT120I GGNCC 1 cut(s) 446
BmiI GGNNCC 3 cut(s) 132, 531, 552
BmrFI CCNGG 8 cut(s) 128, 159, 211, 275, 444, 617, 818, 819
BmrI ACTGGG 3 cut(s) 104, 515, 776
BmsI GCATC 2 cut(s) 269, 1036
BmuI ACTGGG 3 cut(s) 104, 515, 776
BpiI GAAGAC 1 cut(s) 1067
BpmI CTGGAG 1 cut(s) 296
Bpu1102I GCTNAGC 1 cut(s) 287
BpuMI CCSGG 3 cut(s) 128, 818, 819
BsaJI CCNNGG 2 cut(s) 616, 817
Bse1I ACTGG 4 cut(s) 110, 510, 782, 842
Bse3DI GCAATG 1 cut(s) 975
BseBI CCWGG 5 cut(s) 159, 211, 275, 444, 617
BseDI CCNNGG 2 cut(s) 616, 817
BseGI GGATG 2 cut(s) 123, 355
BseMI GCAATG 1 cut(s) 975
BseMII CTCAG 3 cut(s) 132, 162, 630
BseNI ACTGG 4 cut(s) 110, 510, 782, 842
BseRI GAGGAG 2 cut(s) 7, 782
BseSI GKGCMC 1 cut(s) 193
BseXI GCAGC 2 cut(s) 811, 1057
BshFI GGCC 1 cut(s) 447
BshNI GGYRCC 3 cut(s) 130, 529, 550
BsiHKAI GWGCWC 3 cut(s) 147, 386, 950
BsiHKCI CYCGRG 1 cut(s) 817
BsiSI CCGG 2 cut(s) 128, 818
BsiWI CGTACG 1 cut(s) 184
BslFI GGGAC 1 cut(s) 886
BsmAI GTCTC 3 cut(s) 56, 790, 865
BsmFI GGGAC 1 cut(s) 886
BsnI GGCC 1 cut(s) 447
BsoBI CYCGRG 1 cut(s) 817
Bsp1286I GDGCHC 5 cut(s) 8, 147, 193, 386, 950
Bsp143I GATC 5 cut(s) 246, 482, 674, 692, 993
Bsp1720I GCTNAGC 1 cut(s) 287
BspANI GGCC 1 cut(s) 447
BspCNI CTCAG 3 cut(s) 133, 161, 631
BspHI TCATGA 1 cut(s) 706
BspLI GGNNCC 3 cut(s) 132, 531, 552
BspMI ACCTGC 1 cut(s) 82
BspPI GGATC 2 cut(s) 682, 700
BspQI GCTCTTC 1 cut(s) 13
BspT107I GGYRCC 3 cut(s) 130, 529, 550
BspTI CTTAAG 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 975
BsrI ACTGG 4 cut(s) 110, 510, 782, 842
BssECI CCNNGG 2 cut(s) 616, 817
BssMI GATC 5 cut(s) 246, 482, 674, 692, 993
BssSI CACGAG 1 cut(s) 192
Bst2BI CACGAG 1 cut(s) 192
Bst2UI CCWGG 5 cut(s) 159, 211, 275, 444, 617
Bst4CI ACNGT 3 cut(s) 463, 522, 877
Bst6I CTCTTC 4 cut(s) 13, 63, 339, 617
BstAFI CTTAAG 1 cut(s) 242
BstDEI CTNAG 5 cut(s) 141, 148, 287, 639, 761
BstF5I GGATG 2 cut(s) 123, 355
BstH2I RGCGCY 1 cut(s) 978
BstHHI GCGC 1 cut(s) 977
BstKTI GATC 5 cut(s) 249, 485, 677, 695, 996
BstMAI GTCTC 3 cut(s) 56, 790, 865
BstMBI GATC 5 cut(s) 246, 482, 674, 692, 993
BstMWI GCNNNNNNNGC 1 cut(s) 974
BstNI CCWGG 5 cut(s) 159, 211, 275, 444, 617
BstSCI CCNGG 8 cut(s) 126, 157, 209, 273, 442, 615, 816, 817
BstSLI GKGCMC 1 cut(s) 193
BstV1I GCAGC 2 cut(s) 811, 1057
BstV2I GAAGAC 1 cut(s) 1067
BstX2I RGATCY 2 cut(s) 482, 692
BstYI RGATCY 2 cut(s) 482, 692
BsuRI GGCC 1 cut(s) 447
BtsCI GGATG 2 cut(s) 123, 355
BtsIMutI CAGTG 4 cut(s) 344, 503, 684, 716
BveI ACCTGC 1 cut(s) 82
CciI TCATGA 1 cut(s) 706
CfoI GCGC 1 cut(s) 977
Cfr13I GGNCC 1 cut(s) 446
Cfr9I CCCGGG 1 cut(s) 817
Csp6I GTAC 3 cut(s) 185, 551, 835
CviAII CATG 1 cut(s) 707
CviJI RGCY 9 cut(s) 6, 152, 384, 447, 628, 824, 911, 1056, 1079
CviKI_1 RGCY 9 cut(s) 6, 152, 384, 447, 628, 824, 911, 1056, 1079
CviQI GTAC 3 cut(s) 185, 551, 835
DdeI CTNAG 5 cut(s) 141, 148, 287, 639, 761
DpnI GATC 5 cut(s) 248, 484, 676, 694, 995
DpnII GATC 5 cut(s) 246, 482, 674, 692, 993
Eam1104I CTCTTC 4 cut(s) 13, 63, 339, 617
EarI CTCTTC 4 cut(s) 13, 63, 339, 617
Ecl136II GAGCTC 1 cut(s) 384
Eco24I GRGCYC 2 cut(s) 8, 386
Eco53kI GAGCTC 1 cut(s) 384
Eco57I CTGAAG 1 cut(s) 1065
Eco88I CYCGRG 1 cut(s) 817
EcoICRI GAGCTC 1 cut(s) 384
EcoRII CCWGG 5 cut(s) 157, 209, 273, 442, 615
EcoT38I GRGCYC 2 cut(s) 8, 386
FaeI CATG 1 cut(s) 710
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 1 cut(s) 886
FatI CATG 1 cut(s) 706
FauNDI CATATG 1 cut(s) 668
Fnu4HI GCNGC 2 cut(s) 825, 1046
FokI GGATG 2 cut(s) 110, 362
FriOI GRGCYC 2 cut(s) 8, 386
Fsp4HI GCNGC 2 cut(s) 825, 1046
FspBI CTAG 2 cut(s) 266, 800
GlaI GCGC 1 cut(s) 976
GluI GCNGC 2 cut(s) 825, 1046
GsuI CTGGAG 1 cut(s) 296
HaeII RGCGCY 1 cut(s) 978
HaeIII GGCC 1 cut(s) 447
HapII CCGG 2 cut(s) 128, 818
HhaI GCGC 1 cut(s) 977
Hin1II CATG 1 cut(s) 710
Hin6I GCGC 1 cut(s) 975
HinP1I GCGC 1 cut(s) 975
HindIII AAGCTT 1 cut(s) 1077
HinfI GANTC 3 cut(s) 270, 772, 983
HpaII CCGG 2 cut(s) 128, 818
HphI GGTGA 4 cut(s) 63, 241, 416, 445
Hpy166II GTNNAC 1 cut(s) 239
Hpy188I TCNGA 6 cut(s) 63, 142, 369, 480, 1020, 1084
Hpy188III TCNNGA 2 cut(s) 707, 953
Hpy8I GTNNAC 1 cut(s) 239
HpyAV CCTTC 1 cut(s) 41
HpyCH4III ACNGT 3 cut(s) 463, 522, 877
HpyCH4V TGCA 5 cut(s) 233, 260, 827, 943, 1045
HpyF10VI GCNNNNNNNGC 1 cut(s) 974
HpyF3I CTNAG 5 cut(s) 141, 148, 287, 639, 761
Hsp92II CATG 1 cut(s) 710
HspAI GCGC 1 cut(s) 975
KpnI GGTACC 1 cut(s) 554
Kzo9I GATC 5 cut(s) 246, 482, 674, 692, 993
LguI GCTCTTC 1 cut(s) 13
LmnI GCTCC 3 cut(s) 381, 686, 821
Lsp1109I GCAGC 2 cut(s) 811, 1057
LweI GCATC 2 cut(s) 269, 1036
MaeI CTAG 2 cut(s) 266, 800
MaeIII GTNAC 1 cut(s) 451
MalI GATC 5 cut(s) 248, 484, 676, 694, 995
MboI GATC 5 cut(s) 246, 482, 674, 692, 993
MboII GAAGA 7 cut(s) 26, 50, 356, 479, 482, 604, 1072
MfeI CAATTG 2 cut(s) 534, 544
MflI RGATCY 2 cut(s) 482, 692
MhlI GDGCHC 5 cut(s) 8, 147, 193, 386, 950
MluCI AATT 8 cut(s) 294, 534, 544, 727, 732, 807, 896, 1040
MmeI TCCRAC 1 cut(s) 86
MroXI GAANNNNTTC 1 cut(s) 474
MseI TTAA 2 cut(s) 215, 243
MspA1I CMGCKG 1 cut(s) 152
MspCI CTTAAG 1 cut(s) 242
MspI CCGG 2 cut(s) 128, 818
MspR9I CCNGG 8 cut(s) 128, 159, 211, 275, 444, 617, 818, 819
MunI CAATTG 2 cut(s) 534, 544
MvaI CCWGG 5 cut(s) 159, 211, 275, 444, 617
MwoI GCNNNNNNNGC 1 cut(s) 974
NciI CCSGG 3 cut(s) 128, 818, 819
NdeI CATATG 1 cut(s) 668
NdeII GATC 5 cut(s) 246, 482, 674, 692, 993
NlaIII CATG 1 cut(s) 710
NlaIV GGNNCC 3 cut(s) 132, 531, 552
NmuCI GTSAC 1 cut(s) 451
PagI TCATGA 1 cut(s) 706
PaqCI CACCTGC 1 cut(s) 82
PciSI GCTCTTC 1 cut(s) 13
PdmI GAANNNNTTC 1 cut(s) 474
PfeI GAWTC 3 cut(s) 270, 772, 983
Pfl23II CGTACG 1 cut(s) 184
PfoI TCCNGGA 1 cut(s) 273
PkrI GCNGC 2 cut(s) 826, 1047
PsiI TTATAA 1 cut(s) 602
Psp124BI GAGCTC 1 cut(s) 386
Psp6I CCWGG 5 cut(s) 157, 209, 273, 442, 615
PspGI CCWGG 5 cut(s) 157, 209, 273, 442, 615
PspLI CGTACG 1 cut(s) 184
PspN4I GGNNCC 3 cut(s) 132, 531, 552
PspPI GGNCC 1 cut(s) 446
PsuI RGATCY 2 cut(s) 482, 692
PvuII CAGCTG 1 cut(s) 152
RsaI GTAC 3 cut(s) 186, 552, 836
RsaNI GTAC 3 cut(s) 185, 551, 835
SacI GAGCTC 1 cut(s) 386
SapI GCTCTTC 1 cut(s) 13
SaqAI TTAA 2 cut(s) 215, 243
SatI GCNGC 2 cut(s) 825, 1046
Sau3AI GATC 5 cut(s) 246, 482, 674, 692, 993
Sau96I GGNCC 1 cut(s) 446
ScrFI CCNGG 8 cut(s) 128, 159, 211, 275, 444, 617, 818, 819
SduI GDGCHC 5 cut(s) 8, 147, 193, 386, 950
SfaNI GCATC 2 cut(s) 269, 1036
SmaI CCCGGG 1 cut(s) 819
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
SpeI ACTAGT 1 cut(s) 799
Sse9I AATT 8 cut(s) 294, 534, 544, 727, 732, 807, 896, 1040
SspI AATATT 1 cut(s) 364
SspMI CTAG 2 cut(s) 266, 800
SstI GAGCTC 1 cut(s) 386
StyD4I CCNGG 8 cut(s) 126, 157, 209, 273, 442, 615, 816, 817
TaaI ACNGT 3 cut(s) 463, 522, 877
TaqI TCGA 3 cut(s) 417, 954, 992
TasI AATT 8 cut(s) 294, 534, 544, 727, 732, 807, 896, 1040
TfiI GAWTC 3 cut(s) 270, 772, 983
Tru1I TTAA 2 cut(s) 215, 243
Tru9I TTAA 2 cut(s) 215, 243
TscAI CASTG 4 cut(s) 344, 510, 684, 723
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 2 cut(s) 824, 1045
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 5 cut(s) 299, 418, 638, 695, 764
TspMI CCCGGG 1 cut(s) 817
TspRI CASTG 4 cut(s) 344, 510, 684, 723
Vha464I CTTAAG 1 cut(s) 242
XapI RAATTY 2 cut(s) 294, 727
XmaI CCCGGG 1 cut(s) 817
XmnI GAANNNNTTC 1 cut(s) 474
XspI CTAG 2 cut(s) 266, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.