pycom13g02810

cyclin-D1-binding protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002414
Physical Location & Seq
Forward (+)
1930677 .. 1931234
558 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g02810.1

Sequence Viewer

Length: 558 bp
ATGAAGGAGCTTAAGCCAGCTTCCTCAGACCCGACTGATGAATCCTCTATTGAGCCTTCTACTGAAGCAGCGATTGAACCCGATGATGATGAAATTTTAAGTGATGGTGATCTGGGCAATGACTTGTCCCCTGAAGAGATGAGAGTTTCCCAGTTGGCAATTGCCGTTGTGTCGGAGGCTCTTGTAGTGATAAAGGAACTAATTCGCACCATCAGTGGTTTGCTTAAACTGGAAAACCCCAATGGCAGCGGTAATCTTGTGGAATCCTTGGAGAAATTGTTGAAAATGTGTAGAGAAATCGGAATACAAATAGACGAGCTTGGAGCTTGTTTATACCCCCCGCAAGAGGTTCCCGCTATCAAGGCAGCATCAGAGAAAATTTCCAGTTTTGTTGATGATATACAATCTGAATTACAGAATCTTACAGGAAATCTGGAAGCTTTTTGTCAAGCATGCGATTGTTTGAAGAGTTCACTGAGACAGCTCGAATCTGCAGCAGCCTCACCTAGTATTGTTGATTTAGAAACCAGTGTGCAGAATATTGATATGAACTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCIP

Protein Analysis

186

Amino Acids

19.86

Weight (kDa)

4.06

Isoelectric Point (pI)

55.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCIP_C PF20936 28 - 135 7.9e-26 Grap2 and cyclin-D-interacting, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 249, 341, 354
AcsI RAATTY 2 cut(s) 93, 378
AcuI CTGAAG 2 cut(s) 84, 153
AfiI CCNNNNNNNGG 1 cut(s) 346
AflII CTTAAG 1 cut(s) 11
AgsI TTSAA 3 cut(s) 77, 283, 466
AluBI AGCT 6 cut(s) 10, 20, 319, 326, 440, 484
AluI AGCT 6 cut(s) 10, 20, 319, 326, 440, 484
Alw26I GTCTC 1 cut(s) 472
ApeKI GCWGC 5 cut(s) 68, 246, 365, 494, 497
ApoI RAATTY 2 cut(s) 93, 378
Asp700I GAANNNNTTC 1 cut(s) 201
AsuHPI GGTGA 2 cut(s) 119, 495
BbvI GCAGC 5 cut(s) 80, 258, 377, 506, 509
BccI CCATC 2 cut(s) 98, 218
BceAI ACGGC 1 cut(s) 149
BcoDI GTCTC 1 cut(s) 472
BfaI CTAG 1 cut(s) 507
BfmI CTRYAG 1 cut(s) 492
BfrI CTTAAG 1 cut(s) 11
BisI GCNGC 5 cut(s) 69, 247, 366, 495, 498
BlsI GCNGC 5 cut(s) 70, 248, 367, 496, 499
BmiI GGNNCC 1 cut(s) 351
BmrI ACTGGG 1 cut(s) 145
BmsI GCATC 1 cut(s) 377
BmuI ACTGGG 1 cut(s) 145
BsaBI GATNNNNATC 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 267
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bsc4I CCNNNNNNNGG 1 cut(s) 346
Bse1I ACTGG 4 cut(s) 151, 234, 384, 528
Bse3DI GCAATG 1 cut(s) 124
Bse8I GATNNNNATC 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 267
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 1 cut(s) 346
BseMI GCAATG 1 cut(s) 124
BseMII CTCAG 2 cut(s) 39, 467
BseNI ACTGG 4 cut(s) 151, 234, 384, 528
BseXI GCAGC 5 cut(s) 80, 258, 377, 506, 509
BsgI GTGCAG 1 cut(s) 554
BslFI GGGAC 1 cut(s) 112
BslI CCNNNNNNNGG 1 cut(s) 346
BsmAI GTCTC 1 cut(s) 472
BsmFI GGGAC 1 cut(s) 112
Bsp143I GATC 1 cut(s) 109
BspACI CCGC 3 cut(s) 249, 341, 354
BspCNI CTCAG 2 cut(s) 38, 468
BspLI GGNNCC 1 cut(s) 351
BspMAI CTGCAG 1 cut(s) 496
BspTI CTTAAG 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 124
BsrI ACTGG 4 cut(s) 151, 234, 384, 528
BssECI CCNNGG 1 cut(s) 267
BssMI GATC 1 cut(s) 109
BssT1I CCWWGG 1 cut(s) 267
Bst4CI ACNGT 1 cut(s) 554
Bst6I CTCTTC 2 cut(s) 129, 461
BstAFI CTTAAG 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 18, 454
BstDEI CTNAG 2 cut(s) 25, 476
BstKTI GATC 1 cut(s) 112
BstMAI GTCTC 1 cut(s) 472
BstMBI GATC 1 cut(s) 109
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNSI RCATGY 1 cut(s) 456
BstSFI CTRYAG 1 cut(s) 492
BstV1I GCAGC 5 cut(s) 80, 258, 377, 506, 509
BtsIMutI CAGTG 3 cut(s) 220, 473, 535
Cac8I GCNNGC 2 cut(s) 18, 454
CviAII CATG 1 cut(s) 453
DdeI CTNAG 2 cut(s) 25, 476
DpnI GATC 1 cut(s) 111
DpnII GATC 1 cut(s) 109
Eam1104I CTCTTC 2 cut(s) 129, 461
EarI CTCTTC 2 cut(s) 129, 461
Eco130I CCWWGG 1 cut(s) 267
Eco57I CTGAAG 2 cut(s) 84, 153
EcoT14I CCWWGG 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 1 cut(s) 456
FaiI YATR 4 cut(s) 334, 401, 454, 548
FaqI GGGAC 1 cut(s) 112
FatI CATG 1 cut(s) 452
FauI CCCGC 2 cut(s) 348, 361
Fnu4HI GCNGC 5 cut(s) 69, 247, 366, 495, 498
Fsp4HI GCNGC 5 cut(s) 69, 247, 366, 495, 498
FspBI CTAG 1 cut(s) 507
GluI GCNGC 5 cut(s) 69, 247, 366, 495, 498
Hin1II CATG 1 cut(s) 456
HindIII AAGCTT 1 cut(s) 438
HinfI GANTC 4 cut(s) 41, 263, 418, 488
HphI GGTGA 2 cut(s) 119, 495
Hpy166II GTNNAC 1 cut(s) 473
Hpy188I TCNGA 5 cut(s) 28, 175, 302, 373, 409
Hpy188III TCNNGA 1 cut(s) 434
Hpy8I GTNNAC 1 cut(s) 473
HpyAV CCTTC 1 cut(s) 66
HpyCH4III ACNGT 1 cut(s) 554
HpyCH4V TGCA 2 cut(s) 494, 535
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpyF3I CTNAG 2 cut(s) 25, 476
Hsp92II CATG 1 cut(s) 456
Kzo9I GATC 1 cut(s) 109
LmnI GCTCC 2 cut(s) 7, 323
LpnPI CCDG 9 cut(s) 30, 98, 144, 164, 215, 397, 411, 419, 541
Lsp1109I GCAGC 5 cut(s) 80, 258, 377, 506, 509
LweI GCATC 1 cut(s) 377
MaeI CTAG 1 cut(s) 507
MalI GATC 1 cut(s) 111
MboI GATC 1 cut(s) 109
MboII GAAGA 2 cut(s) 146, 478
MfeI CAATTG 1 cut(s) 159
MluCI AATT 6 cut(s) 93, 159, 201, 275, 378, 410
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 5 cut(s) 34, 55, 169, 340, 511
MroXI GAANNNNTTC 1 cut(s) 201
MseI TTAA 3 cut(s) 12, 98, 225
MspA1I CMGCKG 1 cut(s) 249
MspCI CTTAAG 1 cut(s) 11
MunI CAATTG 1 cut(s) 159
MwoI GCNNNNNNNGC 1 cut(s) 362
NdeII GATC 1 cut(s) 109
NlaIII CATG 1 cut(s) 456
NlaIV GGNNCC 1 cut(s) 351
NspI RCATGY 1 cut(s) 456
PaeI GCATGC 1 cut(s) 456
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 4 cut(s) 41, 263, 418, 488
PkrI GCNGC 5 cut(s) 70, 248, 367, 496, 499
PspN4I GGNNCC 1 cut(s) 351
PstI CTGCAG 1 cut(s) 496
SaqAI TTAA 3 cut(s) 12, 98, 225
SatI GCNGC 5 cut(s) 69, 247, 366, 495, 498
Sau3AI GATC 1 cut(s) 109
SetI ASST 8 cut(s) 12, 22, 321, 328, 351, 442, 486, 508
SfaNI GCATC 1 cut(s) 377
SfcI CTRYAG 1 cut(s) 492
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SphI GCATGC 1 cut(s) 456
Sse9I AATT 6 cut(s) 93, 159, 201, 275, 378, 410
SsiI CCGC 3 cut(s) 249, 341, 354
SspI AATATT 1 cut(s) 541
SspMI CTAG 1 cut(s) 507
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 1 cut(s) 554
TaqI TCGA 1 cut(s) 486
TasI AATT 6 cut(s) 93, 159, 201, 275, 378, 410
TfiI GAWTC 4 cut(s) 41, 263, 418, 488
Tru1I TTAA 3 cut(s) 12, 98, 225
Tru9I TTAA 3 cut(s) 12, 98, 225
TscAI CASTG 3 cut(s) 220, 480, 535
TseI GCWGC 5 cut(s) 68, 246, 365, 494, 497
TspDTI ATGAA 3 cut(s) 17, 54, 105
TspRI CASTG 3 cut(s) 220, 480, 535
Vha464I CTTAAG 1 cut(s) 11
XapI RAATTY 2 cut(s) 93, 378
XceI RCATGY 1 cut(s) 456
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.