RchiOBHm_Chr7g0223611

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002414
Physical Location & Seq
Reverse (-)
45444184 .. 45445333
1150 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20021

Sequence Viewer

Length: 651 bp
ATGCTTGGGATACACTTGCAGAGGGCAATGCTTGTTCTTCTGCTGGCCTGCATTCCTATTGCAACTATATGGTTCAATGCAGGCCACATTCTTCAATCCTTGGGTCAAGATCCAGAAATTGCCGCAGCTACTGGAAACTATGCTCGTTTCTTGATACCTTGCATTTTTGCTTACGCAGTCCAACAATGTCATTCTAGATTCTTGCAAACCCAAAACAATGTGGTTCCTATGATAGTTAGCACAGGCACAGCAACACTAGTACACTTGCTTGTCTGTTGGCTTCTGGTATATAAGACAAGCCTTGGATATAGAGGAGCTGCTGTCGCAATCTCCATATCATATTGGTTCAATGCATTGTTCTTGGTTGTTTATATTAGAGTTTCTCCCTCTTGTAAACACACATGGACTGGATTTTCAAAGGAGGCATTTCATGGAATTCCCAACTTCATAAAATTATCTATCCCTTCAGCTATAATGATCAGCCTTAACACATGCTCCATGATATACATGATTCCTCTTGCATTCGCCGCTGCATCAAGCACAAGAGTTTCAAATCAACTGAGTGCCGGGCAACCGCGACTTGCTAGTCTAGCAGTAGGTGTTGCACTATGCATTGTTGTTACCGACGGTATTGTTTGGCTCCAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

216

Amino Acids

23.52

Weight (kDa)

9.23

Isoelectric Point (pI)

28.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 2 - 94 2.7e-17 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 585
AccII CGCG 1 cut(s) 577
AciI CCGC 3 cut(s) 123, 528, 575
AclWI GGATC 1 cut(s) 104
AcsI RAATTY 1 cut(s) 435
AcuI CTGAAG 1 cut(s) 450
AfaI GTAC 1 cut(s) 261
AgsI TTSAA 5 cut(s) 76, 95, 349, 417, 552
AhlI ACTAGT 1 cut(s) 256
AluBI AGCT 3 cut(s) 128, 317, 470
AluI AGCT 3 cut(s) 128, 317, 470
AlwI GGATC 1 cut(s) 104
AlwNI CAGNNNCTG 1 cut(s) 131
AoxI GGCC 2 cut(s) 45, 82
ApeKI GCWGC 3 cut(s) 125, 317, 530
ApoI RAATTY 1 cut(s) 435
AsuC2I CCSGG 1 cut(s) 568
BbvI GCAGC 3 cut(s) 137, 304, 517
BciVI GTATCC 1 cut(s) 3
BclI TGATCA 1 cut(s) 477
BcnI CCSGG 1 cut(s) 568
BcuI ACTAGT 1 cut(s) 256
BfaI CTAG 5 cut(s) 195, 257, 585, 590, 649
BfuI GTATCC 1 cut(s) 3
BisI GCNGC 5 cut(s) 123, 126, 318, 528, 531
BlsI GCNGC 5 cut(s) 124, 127, 319, 529, 532
Bme1390I CCNGG 1 cut(s) 568
BmiI GGNNCC 2 cut(s) 225, 641
BmrFI CCNGG 1 cut(s) 568
BmsI GCATC 1 cut(s) 542
BpuMI CCSGG 1 cut(s) 568
BsaJI CCNNGG 2 cut(s) 99, 301
BsaXI ACNNNNNCTCC 4 cut(s) 306, 336, 479, 509
Bse1I ACTGG 2 cut(s) 136, 412
Bse3DI GCAATG 1 cut(s) 33
BseDI CCNNGG 2 cut(s) 99, 301
BseMI GCAATG 1 cut(s) 33
BseMII CTCAG 1 cut(s) 551
BseNI ACTGG 2 cut(s) 136, 412
BseRI GAGGAG 1 cut(s) 327
BseXI GCAGC 3 cut(s) 137, 304, 517
Bsh1236I CGCG 1 cut(s) 577
BshFI GGCC 2 cut(s) 47, 84
BsiSI CCGG 1 cut(s) 567
BsmI GAATGC 2 cut(s) 51, 521
BsnI GGCC 2 cut(s) 47, 84
Bsp143I GATC 2 cut(s) 109, 477
BspACI CCGC 3 cut(s) 123, 528, 575
BspANI GGCC 2 cut(s) 47, 84
BspCNI CTCAG 1 cut(s) 552
BspFNI CGCG 1 cut(s) 577
BspLI GGNNCC 2 cut(s) 225, 641
BspPI GGATC 1 cut(s) 104
BsrDI GCAATG 1 cut(s) 33
BsrI ACTGG 2 cut(s) 136, 412
BssECI CCNNGG 2 cut(s) 99, 301
BssMI GATC 2 cut(s) 109, 477
BssT1I CCWWGG 2 cut(s) 99, 301
Bst4CI ACNGT 1 cut(s) 629
BstC8I GCNNGC 3 cut(s) 45, 49, 82
BstDEI CTNAG 1 cut(s) 560
BstFNI CGCG 1 cut(s) 577
BstKTI GATC 2 cut(s) 112, 480
BstMBI GATC 2 cut(s) 109, 477
BstMWI GCNNNNNNNGC 3 cut(s) 323, 527, 590
BstNSI RCATGY 1 cut(s) 495
BstSCI CCNGG 1 cut(s) 566
BstUI CGCG 1 cut(s) 577
BstV1I GCAGC 3 cut(s) 137, 304, 517
BstX2I RGATCY 1 cut(s) 109
BstYI RGATCY 1 cut(s) 109
BsuI GTATCC 1 cut(s) 3
BsuRI GGCC 2 cut(s) 47, 84
Cac8I GCNNGC 3 cut(s) 45, 49, 82
CaiI CAGNNNCTG 1 cut(s) 131
Csp6I GTAC 1 cut(s) 260
CviAII CATG 5 cut(s) 402, 431, 492, 499, 508
CviJI RGCY 9 cut(s) 47, 84, 128, 280, 300, 317, 470, 483, 640
CviKI_1 RGCY 9 cut(s) 47, 84, 128, 280, 300, 317, 470, 483, 640
CviQI GTAC 1 cut(s) 260
DdeI CTNAG 1 cut(s) 560
DpnI GATC 2 cut(s) 111, 479
DpnII GATC 2 cut(s) 109, 477
DrdI GACNNNNNNGTC 1 cut(s) 585
DseDI GACNNNNNNGTC 1 cut(s) 585
Eco130I CCWWGG 2 cut(s) 99, 301
Eco57I CTGAAG 1 cut(s) 450
EcoRI GAATTC 1 cut(s) 435
EcoT14I CCWWGG 2 cut(s) 99, 301
EcoT22I ATGCAT 2 cut(s) 355, 614
ErhI CCWWGG 2 cut(s) 99, 301
FaeI CATG 5 cut(s) 405, 434, 495, 502, 511
FatI CATG 5 cut(s) 401, 430, 491, 498, 507
FbaI TGATCA 1 cut(s) 477
Fnu4HI GCNGC 5 cut(s) 123, 126, 318, 528, 531
Fsp4HI GCNGC 5 cut(s) 123, 126, 318, 528, 531
FspBI CTAG 5 cut(s) 195, 257, 585, 590, 649
GluI GCNGC 5 cut(s) 123, 126, 318, 528, 531
HaeIII GGCC 2 cut(s) 47, 84
HapII CCGG 1 cut(s) 567
Hin1II CATG 5 cut(s) 405, 434, 495, 502, 511
HinfI GANTC 2 cut(s) 198, 511
HpaII CCGG 1 cut(s) 567
Hpy166II GTNNAC 2 cut(s) 262, 395
Hpy188III TCNNGA 4 cut(s) 107, 113, 151, 195
Hpy8I GTNNAC 2 cut(s) 262, 395
Hpy99I CGWCG 1 cut(s) 629
HpyAV CCTTC 1 cut(s) 474
HpyCH4III ACNGT 1 cut(s) 629
HpyF10VI GCNNNNNNNGC 3 cut(s) 323, 527, 590
HpyF3I CTNAG 1 cut(s) 560
Hsp92II CATG 5 cut(s) 405, 434, 495, 502, 511
Ksp22I TGATCA 1 cut(s) 477
Kzo9I GATC 2 cut(s) 109, 477
LmnI GCTCC 3 cut(s) 314, 500, 645
LpnPI CCDG 9 cut(s) 29, 61, 66, 117, 126, 228, 269, 393, 580
Lsp1109I GCAGC 3 cut(s) 137, 304, 517
LweI GCATC 1 cut(s) 542
MaeI CTAG 5 cut(s) 195, 257, 585, 590, 649
MaeIII GTNAC 1 cut(s) 619
MalI GATC 2 cut(s) 111, 479
MboI GATC 2 cut(s) 109, 477
MboII GAAGA 2 cut(s) 29, 83
MflI RGATCY 1 cut(s) 109
MluCI AATT 3 cut(s) 117, 435, 452
MmeI TCCRAC 1 cut(s) 205
MnlI CCTC 5 cut(s) 15, 305, 397, 415, 525
Mph1103I ATGCAT 2 cut(s) 355, 614
MseI TTAA 1 cut(s) 486
MspA1I CMGCKG 1 cut(s) 530
MspI CCGG 1 cut(s) 567
MspR9I CCNGG 1 cut(s) 568
Mva1269I GAATGC 2 cut(s) 51, 521
MvnI CGCG 1 cut(s) 577
MwoI GCNNNNNNNGC 3 cut(s) 323, 527, 590
NciI CCSGG 1 cut(s) 568
NdeII GATC 2 cut(s) 109, 477
NlaIII CATG 5 cut(s) 405, 434, 495, 502, 511
NlaIV GGNNCC 2 cut(s) 225, 641
NsiI ATGCAT 2 cut(s) 355, 614
NspI RCATGY 1 cut(s) 495
PctI GAATGC 2 cut(s) 51, 521
PfeI GAWTC 2 cut(s) 198, 511
PkrI GCNGC 5 cut(s) 124, 127, 319, 529, 532
PspN4I GGNNCC 2 cut(s) 225, 641
PstNI CAGNNNCTG 1 cut(s) 131
PsuI RGATCY 1 cut(s) 109
RsaI GTAC 1 cut(s) 261
RsaNI GTAC 1 cut(s) 260
SaqAI TTAA 1 cut(s) 486
SatI GCNGC 5 cut(s) 123, 126, 318, 528, 531
Sau3AI GATC 2 cut(s) 109, 477
ScrFI CCNGG 1 cut(s) 568
SetI ASST 5 cut(s) 130, 160, 319, 472, 601
SfaNI GCATC 1 cut(s) 542
SpeI ACTAGT 1 cut(s) 256
Sse9I AATT 3 cut(s) 117, 435, 452
SsiI CCGC 3 cut(s) 123, 528, 575
SspMI CTAG 5 cut(s) 195, 257, 585, 590, 649
StyD4I CCNGG 1 cut(s) 566
StyI CCWWGG 2 cut(s) 99, 301
TaaI ACNGT 1 cut(s) 629
TasI AATT 3 cut(s) 117, 435, 452
TatI WGTACW 1 cut(s) 259
TauI GCSGC 2 cut(s) 125, 530
TfiI GAWTC 2 cut(s) 198, 511
Tru1I TTAA 1 cut(s) 486
Tru9I TTAA 1 cut(s) 486
TseI GCWGC 3 cut(s) 125, 317, 530
TspDTI ATGAA 2 cut(s) 419, 436
XapI RAATTY 1 cut(s) 435
XbaI TCTAGA 1 cut(s) 194
XceI RCATGY 1 cut(s) 495
XspI CTAG 5 cut(s) 195, 257, 585, 590, 649
Zsp2I ATGCAT 2 cut(s) 355, 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.