pycom13g06230

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002194
Physical Location & Seq
Reverse (-)
4049032 .. 4050076
1045 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 600 bp
ATGGGGAAGACCGGCCTGTTCGATCTCGAAAAGCACTTTGCTTTCTACGGAGCTTACCACAGCAACCCAGTCAACATAGCGATTCACATGCTCTTCGTCTGGCCAATACTTTTCACCTTTCTGCTCATCTTCTACTTCACGCCCTCGCTGCTGGGTTTTGCGGTTTCGGTGTTTGGGAACGACGTCGTTATTCCTTTCAACATCGGCTTGCTGTTGACTATAATCTATTCGGTGTTCTACATCTGTTTGGACGCAAAAGCTGGCTCCTTGGGTGCTCTGCTCTGTGTAATTTGCTGGGTTGCTAGCTGTTTCCTTGGAACCCTACTCGGATTTTCCATCTCTTGGAAGGTTTTTCTGGTGACTCAGATTGTGTGTTGGACTGGACAATTTATTGGCCATGGGGCCTTTGAGAAAAGGGCACCAGCTTTGTTGGACAATCTAGCACAGGCCTTTATAATGGCTCCCTTCTTTGTTCTGTTGGAGGCTCTGCAAATGTTCTTCGGCTACGAGCCCTACCCCGGGTTTCAAGCAATTGTTCAGGCAAAGATTGATGCTGAAATCAGTGAATGGCAAGAGAAGAAGAAGAAACTGATATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

200

Amino Acids

22.31

Weight (kDa)

6.39

Isoelectric Point (pI)

31.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 8 - 164 8.3e-21 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 455
AatII GACGTC 1 cut(s) 186
AccB1I GGYRCC 1 cut(s) 418
AccB7I CCANNNNNTGG 1 cut(s) 342
AciI CCGC 1 cut(s) 161
AcoI YGGCCR 2 cut(s) 101, 394
AcyI GRCGYC 1 cut(s) 183
AfiI CCNNNNNNNGG 3 cut(s) 342, 518, 519
AgsI TTSAA 2 cut(s) 199, 527
AluBI AGCT 4 cut(s) 53, 260, 306, 425
AluI AGCT 4 cut(s) 53, 260, 306, 425
Alw21I GWGCWC 1 cut(s) 277
Ama87I CYCGRG 1 cut(s) 518
AoxI GGCC 5 cut(s) 13, 101, 394, 402, 447
ApeKI GCWGC 1 cut(s) 148
AspS9I GGNCC 1 cut(s) 402
AsuC2I CCSGG 2 cut(s) 519, 520
AsuHPI GGTGA 2 cut(s) 106, 370
AsuNHI GCTAGC 1 cut(s) 302
AvaI CYCGRG 1 cut(s) 518
BaeGI GKGCMC 1 cut(s) 421
BalI TGGCCA 2 cut(s) 103, 396
BanI GGYRCC 1 cut(s) 418
BanII GRGCYC 1 cut(s) 513
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 277
BbvI GCAGC 1 cut(s) 135
BccI CCATC 1 cut(s) 344
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BcnI CCSGG 2 cut(s) 519, 520
BfaI CTAG 2 cut(s) 303, 440
BisI GCNGC 1 cut(s) 149
BlsI GCNGC 1 cut(s) 150
Bme1390I CCNGG 2 cut(s) 519, 520
BmeT110I CYCGRG 1 cut(s) 518
BmgT120I GGNCC 1 cut(s) 402
BmiI GGNNCC 5 cut(s) 265, 319, 403, 420, 462
BmrFI CCNGG 2 cut(s) 519, 520
BmrI ACTGGG 1 cut(s) 62
BmsI GCATC 1 cut(s) 541
BmtI GCTAGC 1 cut(s) 306
BmuI ACTGGG 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 14
BpuMI CCSGG 2 cut(s) 519, 520
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 5 cut(s) 267, 313, 397, 517, 518
Bsc4I CCNNNNNNNGG 3 cut(s) 342, 518, 519
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 2 cut(s) 68, 385
BseDI CCNNGG 5 cut(s) 267, 313, 397, 517, 518
BseLI CCNNNNNNNGG 3 cut(s) 342, 518, 519
BseMII CTCAG 1 cut(s) 377
BseNI ACTGG 2 cut(s) 68, 385
BseSI GKGCMC 1 cut(s) 421
BseXI GCAGC 1 cut(s) 135
BseYI CCCAGC 2 cut(s) 151, 294
BshFI GGCC 5 cut(s) 15, 103, 396, 404, 449
BshNI GGYRCC 1 cut(s) 418
BsiHKAI GWGCWC 1 cut(s) 277
BsiHKCI CYCGRG 1 cut(s) 518
BsiSI CCGG 2 cut(s) 12, 519
BslI CCNNNNNNNGG 3 cut(s) 342, 518, 519
BsnI GGCC 5 cut(s) 15, 103, 396, 404, 449
BsoBI CYCGRG 1 cut(s) 518
Bsp1286I GDGCHC 3 cut(s) 277, 421, 513
Bsp143I GATC 1 cut(s) 22
Bsp19I CCATGG 1 cut(s) 397
BspACI CCGC 1 cut(s) 161
BspANI GGCC 5 cut(s) 15, 103, 396, 404, 449
BspCNI CTCAG 1 cut(s) 376
BspLI GGNNCC 5 cut(s) 265, 319, 403, 420, 462
BspOI GCTAGC 1 cut(s) 306
BspQI GCTCTTC 1 cut(s) 98
BspT107I GGYRCC 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 11
BsrI ACTGG 2 cut(s) 68, 385
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 5 cut(s) 267, 313, 397, 517, 518
BssMI GATC 1 cut(s) 22
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 3 cut(s) 267, 313, 397
Bst6I CTCTTC 1 cut(s) 98
BstACI GRCGYC 1 cut(s) 183
BstC8I GCNNGC 3 cut(s) 209, 262, 304
BstDEI CTNAG 1 cut(s) 363
BstDSI CCRYGG 1 cut(s) 397
BstKTI GATC 1 cut(s) 25
BstMBI GATC 1 cut(s) 22
BstMWI GCNNNNNNNGC 1 cut(s) 148
BstNSI RCATGY 1 cut(s) 91
BstSCI CCNGG 2 cut(s) 517, 518
BstSLI GKGCMC 1 cut(s) 421
BstV1I GCAGC 1 cut(s) 135
BstV2I GAAGAC 1 cut(s) 14
BsuRI GGCC 5 cut(s) 15, 103, 396, 404, 449
BtgI CCRYGG 1 cut(s) 397
BtsIMutI CAGTG 1 cut(s) 568
Cac8I GCNNGC 3 cut(s) 209, 262, 304
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 402
Cfr9I CCCGGG 1 cut(s) 518
CseI GACGC 1 cut(s) 260
CviAII CATG 2 cut(s) 88, 398
DdeI CTNAG 1 cut(s) 363
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
EaeI YGGCCR 2 cut(s) 101, 394
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Eco130I CCWWGG 3 cut(s) 267, 313, 397
Eco147I AGGCCT 1 cut(s) 449
Eco24I GRGCYC 1 cut(s) 513
Eco32I GATATC 1 cut(s) 594
Eco88I CYCGRG 1 cut(s) 518
EcoO109I RGGNCCY 1 cut(s) 402
EcoRV GATATC 1 cut(s) 594
EcoT14I CCWWGG 3 cut(s) 267, 313, 397
EcoT38I GRGCYC 1 cut(s) 513
ErhI CCWWGG 3 cut(s) 267, 313, 397
FaeI CATG 2 cut(s) 91, 401
FaiI YATR 5 cut(s) 77, 89, 221, 399, 455
FatI CATG 2 cut(s) 87, 397
Fnu4HI GCNGC 1 cut(s) 149
FriOI GRGCYC 1 cut(s) 513
Fsp4HI GCNGC 1 cut(s) 149
FspBI CTAG 2 cut(s) 303, 440
GluI GCNGC 1 cut(s) 149
GsaI CCCAGC 2 cut(s) 155, 298
HaeIII GGCC 5 cut(s) 15, 103, 396, 404, 449
HapII CCGG 2 cut(s) 12, 519
HgaI GACGC 1 cut(s) 260
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 2 cut(s) 91, 401
HincII GTYRAC 2 cut(s) 73, 216
HindII GTYRAC 2 cut(s) 73, 216
HinfI GANTC 2 cut(s) 82, 361
HpaII CCGG 2 cut(s) 12, 519
HphI GGTGA 2 cut(s) 106, 370
Hpy166II GTNNAC 2 cut(s) 73, 216
Hpy188I TCNGA 2 cut(s) 329, 366
Hpy188III TCNNGA 1 cut(s) 26
Hpy8I GTNNAC 2 cut(s) 73, 216
Hpy99I CGWCG 2 cut(s) 185, 188
HpyAV CCTTC 2 cut(s) 340, 475
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 1 cut(s) 490
HpyF10VI GCNNNNNNNGC 1 cut(s) 148
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 1 cut(s) 183
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 2 cut(s) 91, 401
Kzo9I GATC 1 cut(s) 22
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 3 cut(s) 50, 269, 466
Lsp1109I GCAGC 1 cut(s) 135
LweI GCATC 1 cut(s) 541
MaeI CTAG 2 cut(s) 303, 440
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 1 cut(s) 358
MalI GATC 1 cut(s) 24
MboI GATC 1 cut(s) 22
MboII GAAGA 7 cut(s) 19, 85, 121, 490, 589, 592, 595
MfeI CAATTG 1 cut(s) 531
MhlI GDGCHC 3 cut(s) 277, 421, 513
MlsI TGGCCA 2 cut(s) 103, 396
MluCI AATT 3 cut(s) 288, 386, 531
MluNI TGGCCA 2 cut(s) 103, 396
MlyI GAGTC 1 cut(s) 355
MmeI TCCRAC 3 cut(s) 356, 411, 459
MnlI CCTC 2 cut(s) 154, 475
Mox20I TGGCCA 2 cut(s) 103, 396
MscI TGGCCA 2 cut(s) 103, 396
Msp20I TGGCCA 2 cut(s) 103, 396
MspI CCGG 2 cut(s) 12, 519
MspR9I CCNGG 2 cut(s) 519, 520
MunI CAATTG 1 cut(s) 531
MwoI GCNNNNNNNGC 1 cut(s) 148
NciI CCSGG 2 cut(s) 519, 520
NcoI CCATGG 1 cut(s) 397
NdeII GATC 1 cut(s) 22
NheI GCTAGC 1 cut(s) 302
NlaIII CATG 2 cut(s) 91, 401
NlaIV GGNNCC 5 cut(s) 265, 319, 403, 420, 462
NmuCI GTSAC 1 cut(s) 358
NspI RCATGY 1 cut(s) 91
PceI AGGCCT 1 cut(s) 449
PciSI GCTCTTC 1 cut(s) 98
PfeI GAWTC 1 cut(s) 82
PflMI CCANNNNNTGG 1 cut(s) 342
PkrI GCNGC 1 cut(s) 150
PleI GAGTC 1 cut(s) 355
PpsI GAGTC 1 cut(s) 355
PsiI TTATAA 1 cut(s) 455
PspFI CCCAGC 2 cut(s) 151, 294
PspN4I GGNNCC 5 cut(s) 265, 319, 403, 420, 462
PspPI GGNCC 1 cut(s) 402
SapI GCTCTTC 1 cut(s) 98
SatI GCNGC 1 cut(s) 149
Sau3AI GATC 1 cut(s) 22
Sau96I GGNCC 1 cut(s) 402
SchI GAGTC 1 cut(s) 355
ScrFI CCNGG 2 cut(s) 519, 520
SduI GDGCHC 3 cut(s) 277, 421, 513
SetI ASST 7 cut(s) 55, 119, 186, 262, 308, 351, 427
SfaNI GCATC 1 cut(s) 541
SmaI CCCGGG 1 cut(s) 520
Sse9I AATT 3 cut(s) 288, 386, 531
SseBI AGGCCT 1 cut(s) 449
SsiI CCGC 1 cut(s) 161
SspMI CTAG 2 cut(s) 303, 440
StuI AGGCCT 1 cut(s) 449
StyD4I CCNGG 2 cut(s) 517, 518
StyI CCWWGG 3 cut(s) 267, 313, 397
TaiI ACGT 1 cut(s) 186
TaqI TCGA 2 cut(s) 21, 27
TasI AATT 3 cut(s) 288, 386, 531
TfiI GAWTC 1 cut(s) 82
TscAI CASTG 1 cut(s) 568
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 1 cut(s) 148
Tsp45I GTSAC 1 cut(s) 358
TspGWI ACGGA 1 cut(s) 63
TspMI CCCGGG 1 cut(s) 518
TspRI CASTG 1 cut(s) 568
Van91I CCANNNNNTGG 1 cut(s) 342
XceI RCATGY 1 cut(s) 91
XmaI CCCGGG 1 cut(s) 518
XspI CTAG 2 cut(s) 303, 440
ZraI GACGTC 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.