pycom14g04730

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001285
Physical Location & Seq
Reverse (-)
4461269 .. 4464134
2866 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 891 bp
ATGGATACTCGTAAAAGAGGGCGGCATGAAGGTGGCGGTCTTCACTCTAATGGCGGCTTCAAGAAGAACAAGCAAGAATTGGAGTCCTTTTCAACCGGTGTAGGAAGCAAATCGAAGCCATGCACCAAATTTTTCAGCACTGCTGGCTGTCCTTTTGGCGAGAGTTGCCACTTCCTGCACTATGTTCCTGGAGGTTACAATGCCGTGGCTCAGATGATGAATCTTGCGCCTGCCCCTCCGCCACCTAGGAACATTCCTGGCCCACCATCCCATTCCAATGGGTCCAATTCATCTGGAGTTAAATCGCGCATATGCAATAAATATAATTCTGCTGAAGGCTGCAAATTTGGTGACAAATGTCATTTTGCTCATGGTGAGTGGGAACTTGGCAAGCCTCTTGCTCAGTCCCACGATGATCCTCATGCCATGGGACCTGGTCCAGGCCGTATGGGTAATTGGATGGAGCCACACCCGGCACGCCCAGCATCTAGCTTTGGTGCCTCATCTACTGCAAAAATCAGTGTGGATGCATCACTTGCAGGAGCCATTATTGGGAAGGGAGGTGTACATTCGAAGCAGATTTGTCGCCAGACAGGAGCCAAGCTTTCAATCCGGGACCATGAGTCAAATCCCAATCTCAGGAACATTGAACTGGAGGGGACCTTTGAACAGATTCAGCAAGCTAGTGCCATGGTGAGTGAGCTAATTGCGACTGTTTCAATCTCTGGCCCTGGTAAGGCTCCGGGAGGAGGAGGCCCAGGAGCCCCAGCCCCTCCAGGAAGTAACTATAAGACGAAACTGTGTGATAATTTTACCAAAGGGACTTGCACCTTTGGAGAAAGATGTCACTTTGCACACGGGGCTGCTGAATTGCGGAAGTCTGGAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1 zf-CCCH zf-CCCH_2

Protein Analysis

297

Amino Acids

30.98

Weight (kDa)

9.24

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_1 PF00013 170 - 233 1.2e-13 KH domain
zf-CCCH PF00642 263 - 287 7.6e-09 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 497
AccII CGCG 1 cut(s) 307
AciI CCGC 5 cut(s) 22, 36, 54, 239, 874
AclWI GGATC 1 cut(s) 410
AcsI RAATTY 2 cut(s) 128, 344
AcuI CTGAAG 1 cut(s) 354
AfaI GTAC 1 cut(s) 567
AfiI CCNNNNNNNGG 5 cut(s) 440, 552, 639, 736, 749
AgeI ACCGGT 1 cut(s) 95
AgsI TTSAA 6 cut(s) 61, 93, 609, 650, 668, 720
AhdI GACNNNNNGTC 1 cut(s) 622
AjnI CCWGG 7 cut(s) 187, 256, 433, 439, 730, 757, 775
AluBI AGCT 4 cut(s) 492, 604, 683, 703
AluI AGCT 4 cut(s) 492, 604, 683, 703
AlwI GGATC 1 cut(s) 410
AoxI GGCC 4 cut(s) 259, 442, 727, 754
ApeKI GCWGC 2 cut(s) 339, 863
ApoI RAATTY 2 cut(s) 128, 344
AsiGI ACCGGT 1 cut(s) 95
Asp700I GAANNNNTTC 1 cut(s) 672
AspA2I CCTAGG 1 cut(s) 245
AspLEI GCGC 2 cut(s) 229, 309
AspS9I GGNCC 8 cut(s) 260, 282, 431, 437, 616, 660, 728, 755
AsuC2I CCSGG 3 cut(s) 473, 614, 744
AsuHPI GGTGA 3 cut(s) 362, 386, 706
AsuII TTCGAA 1 cut(s) 572
AvaII GGWCC 5 cut(s) 282, 431, 437, 616, 660
AvrII CCTAGG 1 cut(s) 245
BanI GGYRCC 1 cut(s) 497
BanII GRGCYC 1 cut(s) 766
BbsI GAAGAC 1 cut(s) 32
BbvI GCAGC 2 cut(s) 326, 850
BccI CCATC 2 cut(s) 274, 454
BceAI ACGGC 2 cut(s) 188, 429
BcgI CGANNNNNNTGC 2 cut(s) 566, 600
BciT130I CCWGG 7 cut(s) 189, 258, 435, 441, 732, 759, 777
BcnI CCSGG 3 cut(s) 473, 614, 744
BfaI CTAG 3 cut(s) 246, 489, 684
BisI GCNGC 4 cut(s) 23, 55, 340, 864
BlnI CCTAGG 1 cut(s) 245
BlsI GCNGC 4 cut(s) 24, 56, 341, 865
Bme18I GGWCC 5 cut(s) 282, 431, 437, 616, 660
BmeRI GACNNNNNGTC 1 cut(s) 622
BmgT120I GGNCC 8 cut(s) 260, 282, 431, 437, 616, 660, 728, 755
BmsI GCATC 3 cut(s) 494, 517, 539
BoxI GACNNNNGTC 1 cut(s) 357
BpiI GAAGAC 1 cut(s) 32
BpmI CTGGAG 4 cut(s) 210, 315, 674, 759
Bpu14I TTCGAA 1 cut(s) 572
BpuMI CCSGG 3 cut(s) 473, 614, 744
BsaJI CCNNGG 6 cut(s) 204, 245, 426, 690, 730, 757
BsaWI WCCGGW 1 cut(s) 95
Bsc4I CCNNNNNNNGG 5 cut(s) 440, 552, 639, 736, 749
Bse118I RCCGGY 1 cut(s) 95
Bse1I ACTGG 1 cut(s) 657
BseBI CCWGG 7 cut(s) 189, 258, 435, 441, 732, 759, 777
BseDI CCNNGG 6 cut(s) 204, 245, 426, 690, 730, 757
BseGI GGATG 3 cut(s) 266, 465, 532
BseLI CCNNNNNNNGG 5 cut(s) 440, 552, 639, 736, 749
BseMII CTCAG 3 cut(s) 224, 416, 652
BseNI ACTGG 1 cut(s) 657
BseRI GAGGAG 2 cut(s) 762, 765
BseXI GCAGC 2 cut(s) 326, 850
BseYI CCCAGC 2 cut(s) 481, 766
BsgI GTGCAG 1 cut(s) 161
Bsh1236I CGCG 1 cut(s) 307
BshFI GGCC 4 cut(s) 261, 444, 729, 756
BshNI GGYRCC 1 cut(s) 497
BshTI ACCGGT 1 cut(s) 95
BsiSI CCGG 4 cut(s) 96, 473, 613, 743
BslFI GGGAC 5 cut(s) 391, 444, 629, 673, 835
BslI CCNNNNNNNGG 5 cut(s) 440, 552, 639, 736, 749
BsmFI GGGAC 5 cut(s) 391, 444, 629, 673, 835
BsnI GGCC 4 cut(s) 261, 444, 729, 756
Bsp119I TTCGAA 1 cut(s) 572
Bsp1286I GDGCHC 1 cut(s) 766
Bsp1407I TGTACA 1 cut(s) 565
Bsp143I GATC 1 cut(s) 415
Bsp19I CCATGG 2 cut(s) 426, 690
BspACI CCGC 5 cut(s) 22, 36, 54, 239, 874
BspANI GGCC 4 cut(s) 261, 444, 729, 756
BspCNI CTCAG 3 cut(s) 223, 415, 651
BspFNI CGCG 1 cut(s) 307
BspPI GGATC 1 cut(s) 410
BspT104I TTCGAA 1 cut(s) 572
BspT107I GGYRCC 1 cut(s) 497
BsrFI RCCGGY 1 cut(s) 95
BsrGI TGTACA 1 cut(s) 565
BsrI ACTGG 1 cut(s) 657
BssAI RCCGGY 1 cut(s) 95
BssECI CCNNGG 6 cut(s) 204, 245, 426, 690, 730, 757
BssMI GATC 1 cut(s) 415
BssT1I CCWWGG 3 cut(s) 245, 426, 690
Bst2UI CCWGG 7 cut(s) 189, 258, 435, 441, 732, 759, 777
Bst4CI ACNGT 2 cut(s) 715, 801
BstAPI GCANNNNNTGC 1 cut(s) 536
BstAUI TGTACA 1 cut(s) 565
BstBI TTCGAA 1 cut(s) 572
BstC8I GCNNGC 5 cut(s) 145, 231, 392, 478, 681
BstDEI CTNAG 3 cut(s) 210, 402, 638
BstDSI CCRYGG 3 cut(s) 204, 426, 690
BstENI CCTNNNNNAGG 1 cut(s) 438
BstF5I GGATG 3 cut(s) 266, 465, 532
BstFNI CGCG 1 cut(s) 307
BstHHI GCGC 2 cut(s) 229, 309
BstKTI GATC 1 cut(s) 418
BstMBI GATC 1 cut(s) 415
BstMWI GCNNNNNNNGC 5 cut(s) 144, 165, 482, 536, 860
BstNI CCWGG 7 cut(s) 189, 258, 435, 441, 732, 759, 777
BstPAI GACNNNNGTC 1 cut(s) 357
BstUI CGCG 1 cut(s) 307
BstV1I GCAGC 2 cut(s) 326, 850
BstV2I GAAGAC 1 cut(s) 32
BstXI CCANNNNNNTGG 1 cut(s) 278
BsuRI GGCC 4 cut(s) 261, 444, 729, 756
BtgI CCRYGG 3 cut(s) 204, 426, 690
BtsCI GGATG 3 cut(s) 266, 465, 532
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 2 cut(s) 138, 526
Cac8I GCNNGC 5 cut(s) 145, 231, 392, 478, 681
CfoI GCGC 2 cut(s) 229, 309
Cfr10I RCCGGY 1 cut(s) 95
Cfr13I GGNCC 8 cut(s) 260, 282, 431, 437, 616, 660, 728, 755
CsiI ACCWGGT 1 cut(s) 433
Csp6I GTAC 1 cut(s) 566
CspAI ACCGGT 1 cut(s) 95
CviAII CATG 7 cut(s) 26, 120, 371, 422, 427, 620, 691
CviQI GTAC 1 cut(s) 566
DdeI CTNAG 3 cut(s) 210, 402, 638
DpnI GATC 1 cut(s) 417
DpnII GATC 1 cut(s) 415
DriI GACNNNNNGTC 1 cut(s) 622
Eam1105I GACNNNNNGTC 1 cut(s) 622
EciI GGCGGA 1 cut(s) 228
Eco130I CCWWGG 3 cut(s) 245, 426, 690
Eco24I GRGCYC 1 cut(s) 766
Eco47I GGWCC 5 cut(s) 282, 431, 437, 616, 660
Eco57I CTGAAG 1 cut(s) 354
EcoNI CCTNNNNNAGG 1 cut(s) 438
EcoO109I RGGNCCY 2 cut(s) 431, 660
EcoRII CCWGG 7 cut(s) 187, 256, 433, 439, 730, 757, 775
EcoT14I CCWWGG 3 cut(s) 245, 426, 690
EcoT22I ATGCAT 1 cut(s) 532
EcoT38I GRGCYC 1 cut(s) 766
ErhI CCWWGG 3 cut(s) 245, 426, 690
FaeI CATG 7 cut(s) 29, 123, 374, 425, 430, 623, 694
FaqI GGGAC 5 cut(s) 391, 444, 629, 673, 835
FatI CATG 7 cut(s) 25, 119, 370, 421, 426, 619, 690
FauNDI CATATG 1 cut(s) 311
Fnu4HI GCNGC 4 cut(s) 23, 55, 340, 864
FokI GGATG 3 cut(s) 253, 472, 539
FriOI GRGCYC 1 cut(s) 766
Fsp4HI GCNGC 4 cut(s) 23, 55, 340, 864
FspBI CTAG 3 cut(s) 246, 489, 684
GlaI GCGC 2 cut(s) 228, 308
GluI GCNGC 4 cut(s) 23, 55, 340, 864
GsaI CCCAGC 2 cut(s) 485, 770
GsuI CTGGAG 4 cut(s) 210, 315, 674, 759
HaeIII GGCC 4 cut(s) 261, 444, 729, 756
HapII CCGG 4 cut(s) 96, 473, 613, 743
HhaI GCGC 2 cut(s) 229, 309
Hin1II CATG 7 cut(s) 29, 123, 374, 425, 430, 623, 694
Hin6I GCGC 2 cut(s) 227, 307
HinP1I GCGC 2 cut(s) 227, 307
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 4 cut(s) 83, 220, 623, 673
HpaII CCGG 4 cut(s) 96, 473, 613, 743
HphI GGTGA 3 cut(s) 362, 386, 706
Hpy166II GTNNAC 1 cut(s) 566
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 4 cut(s) 61, 294, 640, 882
Hpy8I GTNNAC 1 cut(s) 566
HpyAV CCTTC 3 cut(s) 23, 329, 550
HpyCH4III ACNGT 2 cut(s) 715, 801
HpyCH4V TGCA 9 cut(s) 123, 178, 315, 342, 512, 530, 539, 828, 854
HpyF10VI GCNNNNNNNGC 5 cut(s) 144, 165, 482, 536, 860
HpyF3I CTNAG 3 cut(s) 210, 402, 638
Hsp92II CATG 7 cut(s) 29, 123, 374, 425, 430, 623, 694
HspAI GCGC 2 cut(s) 227, 307
Kzo9I GATC 1 cut(s) 415
LmnI GCTCC 5 cut(s) 463, 542, 596, 745, 761
Lsp1109I GCAGC 2 cut(s) 326, 850
LweI GCATC 3 cut(s) 494, 517, 539
MabI ACCWGGT 1 cut(s) 433
MaeI CTAG 3 cut(s) 246, 489, 684
MaeIII GTNAC 4 cut(s) 194, 350, 782, 845
MalI GATC 1 cut(s) 417
MboI GATC 1 cut(s) 415
MboII GAAGA 2 cut(s) 32, 76
MhlI GDGCHC 1 cut(s) 766
MluCI AATT 9 cut(s) 77, 128, 286, 325, 344, 454, 705, 808, 869
MlyI GAGTC 2 cut(s) 92, 632
Mph1103I ATGCAT 1 cut(s) 532
MroXI GAANNNNTTC 1 cut(s) 672
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 3 cut(s) 30, 48, 276
MspI CCGG 4 cut(s) 96, 473, 613, 743
MvaI CCWGG 7 cut(s) 189, 258, 435, 441, 732, 759, 777
MvnI CGCG 1 cut(s) 307
MwoI GCNNNNNNNGC 5 cut(s) 144, 165, 482, 536, 860
NciI CCSGG 3 cut(s) 473, 614, 744
NcoI CCATGG 2 cut(s) 426, 690
NdeI CATATG 1 cut(s) 311
NdeII GATC 1 cut(s) 415
NlaIII CATG 7 cut(s) 29, 123, 374, 425, 430, 623, 694
NmuCI GTSAC 2 cut(s) 350, 845
NsiI ATGCAT 1 cut(s) 532
NspV TTCGAA 1 cut(s) 572
PdmI GAANNNNTTC 1 cut(s) 672
PfeI GAWTC 2 cut(s) 220, 673
PflFI GACNNNGTC 1 cut(s) 435
PfoI TCCNGGA 4 cut(s) 187, 612, 742, 775
PinAI ACCGGT 1 cut(s) 95
PkrI GCNGC 4 cut(s) 24, 56, 341, 865
PleI GAGTC 2 cut(s) 91, 631
PpsI GAGTC 2 cut(s) 91, 631
PpuMI RGGWCCY 2 cut(s) 431, 660
PshAI GACNNNNGTC 1 cut(s) 357
Psp5II RGGWCCY 2 cut(s) 431, 660
Psp6I CCWGG 7 cut(s) 187, 256, 433, 439, 730, 757, 775
PspFI CCCAGC 2 cut(s) 481, 766
PspGI CCWGG 7 cut(s) 187, 256, 433, 439, 730, 757, 775
PspPI GGNCC 8 cut(s) 260, 282, 431, 437, 616, 660, 728, 755
PspPPI RGGWCCY 2 cut(s) 431, 660
PsyI GACNNNGTC 1 cut(s) 435
RsaI GTAC 1 cut(s) 567
RsaNI GTAC 1 cut(s) 566
RseI CAYNNNNRTG 3 cut(s) 30, 48, 276
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 4 cut(s) 23, 55, 340, 864
Sau3AI GATC 1 cut(s) 415
Sau96I GGNCC 8 cut(s) 260, 282, 431, 437, 616, 660, 728, 755
SchI GAGTC 2 cut(s) 92, 632
SduI GDGCHC 1 cut(s) 766
SexAI ACCWGGT 1 cut(s) 433
SfaNI GCATC 3 cut(s) 494, 517, 539
SfuI TTCGAA 1 cut(s) 572
SinI GGWCC 5 cut(s) 282, 431, 437, 616, 660
SmiMI CAYNNNNRTG 3 cut(s) 30, 48, 276
Sse9I AATT 9 cut(s) 77, 128, 286, 325, 344, 454, 705, 808, 869
SsiI CCGC 5 cut(s) 22, 36, 54, 239, 874
SspMI CTAG 3 cut(s) 246, 489, 684
StyI CCWWGG 3 cut(s) 245, 426, 690
TaaI ACNGT 2 cut(s) 715, 801
TaqI TCGA 2 cut(s) 113, 572
TasI AATT 9 cut(s) 77, 128, 286, 325, 344, 454, 705, 808, 869
TatI WGTACW 1 cut(s) 565
TauI GCSGC 2 cut(s) 25, 57
TfiI GAWTC 2 cut(s) 220, 673
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 2 cut(s) 145, 526
TseFI GTSAC 2 cut(s) 350, 845
TseI GCWGC 2 cut(s) 339, 863
Tsp45I GTSAC 2 cut(s) 350, 845
TspDTI ATGAA 3 cut(s) 42, 233, 279
TspRI CASTG 2 cut(s) 145, 526
Tth111I GACNNNGTC 1 cut(s) 435
VpaK11BI GGWCC 5 cut(s) 282, 431, 437, 616, 660
XagI CCTNNNNNAGG 1 cut(s) 438
XapI RAATTY 2 cut(s) 128, 344
XmaJI CCTAGG 1 cut(s) 245
XmnI GAANNNNTTC 1 cut(s) 672
XspI CTAG 3 cut(s) 246, 489, 684
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.