pycom14g05010

60s ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0002002
Physical Location & Seq
Forward (+)
4828532 .. 4830775
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g05010.2

Sequence Viewer

Length: 702 bp
ATGGCGGCTAAAGCAAGAAACCCGAGAAAGACCCGAAACCCAGATCTGGTTCGTGGGGTGGGTAAGTTTTCGAGGTCCAAGATGTACCATAAGCGTGGTCTCTGGGCTATCAAGGCCAAAAATGGCGGCGTTTTTCCAAGCCATGACCCGAAGCCGGCGGCCGAGATCGCATCCGAGAAGCCCCCGAAGTTCTACCCCGCCGATGACGTCAAGAAGCCGCTCGTCAACAAACGCAAGCCAAAACCCACCACGCTCAGGGCGAGTATTACACCGGGGACGGTGTTGATTATATTGTCTGGGAGGTTTAAGGGCAAGAGAGTTGTTTTTCTTAAGCAGCTTCCATCTGGGTTGCTTCTTGTTACTGGGCCCTTTAAAATTAATGGTGTTCCTTTGAGACGCGTTAATCAGTCTTATGTGATTGGCACTTCAACCAAGGTTGACATTTCTGGGGTGAATGTAGAAAAGTTTGATGACAAATATTTTGCGAAGGAAGTTGAAAAGAAGAAGAAGAAGGGTGAGGGAGAGTTTTTTGAGGCAGAGAAAGAGGAAAAGAGTGTGCTCCCACAAGGGAAGAAAGATGATCAGAAGAGTGTGGATGCTTCATTGATGAAGTCCATCGAGGCTGTCCCAGACTTGAAGACATACTTAGCAGCGAGATTCTCTCTTAAGGCGGGCATGAAGCCTCATGAGCTTATCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L6e Ribosomal_L6e_N

Protein Analysis

234

Amino Acids

25.95

Weight (kDa)

10.16

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 210
AccBSI CCGCTC 1 cut(s) 220
AccII CGCG 1 cut(s) 399
AciI CCGC 6 cut(s) 5, 126, 158, 198, 218, 671
AcoI YGGCCR 1 cut(s) 159
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 3 cut(s) 46, 154, 255
AflII CTTAAG 2 cut(s) 329, 665
AflIII ACRYGT 1 cut(s) 397
AgsI TTSAA 3 cut(s) 429, 497, 637
AluBI AGCT 2 cut(s) 337, 691
AluI AGCT 2 cut(s) 337, 691
Alw21I GWGCWC 1 cut(s) 561
Alw26I GTCTC 2 cut(s) 104, 388
Ama87I CYCGRG 1 cut(s) 22
AoxI GGCC 3 cut(s) 114, 159, 365
ApaI GGGCCC 1 cut(s) 369
ApeKI GCWGC 2 cut(s) 334, 650
ArsI GACNNNNNNTTYG 2 cut(s) 464, 496
AseI ATTAAT 1 cut(s) 378
AspS9I GGNCC 3 cut(s) 75, 365, 366
AsuC2I CCSGG 1 cut(s) 273
AsuHPI GGTGA 2 cut(s) 463, 527
AvaI CYCGRG 1 cut(s) 22
AvaII GGWCC 1 cut(s) 75
BaeGI GKGCMC 1 cut(s) 369
BanII GRGCYC 1 cut(s) 369
BbsI GAAGAC 1 cut(s) 644
Bbv12I GWGCWC 1 cut(s) 561
BbvI GCAGC 2 cut(s) 346, 662
BccI CCATC 2 cut(s) 349, 623
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 1 cut(s) 273
BcoDI GTCTC 2 cut(s) 104, 388
BfrI CTTAAG 2 cut(s) 329, 665
BglII AGATCT 1 cut(s) 43
BisI GCNGC 6 cut(s) 6, 127, 159, 218, 335, 651
BlsI GCNGC 6 cut(s) 7, 128, 160, 219, 336, 652
Bme1390I CCNGG 1 cut(s) 273
Bme18I GGWCC 1 cut(s) 75
BmeT110I CYCGRG 1 cut(s) 22
BmgT120I GGNCC 3 cut(s) 75, 365, 366
BmiI GGNNCC 1 cut(s) 367
BmrFI CCNGG 1 cut(s) 273
BmrI ACTGGG 1 cut(s) 372
BmsI GCATC 2 cut(s) 179, 586
BmuI ACTGGG 1 cut(s) 372
BpiI GAAGAC 1 cut(s) 644
BplI GAGNNNNNCTC 2 cut(s) 646, 678
Bpu10I CCTNAGC 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 273
BsaHI GRCGYC 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 2 cut(s) 272, 432
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 154, 255
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 1 cut(s) 367
BseDI CCNNGG 2 cut(s) 272, 432
BseGI GGATG 2 cut(s) 170, 601
BseLI CCNNNNNNNGG 3 cut(s) 46, 154, 255
BseMII CTCAG 1 cut(s) 268
BseNI ACTGG 1 cut(s) 367
BseSI GKGCMC 1 cut(s) 369
BseX3I CGGCCG 1 cut(s) 159
BseXI GCAGC 2 cut(s) 346, 662
Bsh1236I CGCG 1 cut(s) 399
Bsh1285I CGRYCG 1 cut(s) 162
BshFI GGCC 3 cut(s) 116, 161, 367
BsiEI CGRYCG 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 561
BsiHKCI CYCGRG 1 cut(s) 22
BsiSI CCGG 2 cut(s) 155, 272
BslFI GGGAC 2 cut(s) 289, 611
BslI CCNNNNNNNGG 3 cut(s) 46, 154, 255
BsmAI GTCTC 2 cut(s) 104, 388
BsmBI CGTCTC 1 cut(s) 388
BsmFI GGGAC 2 cut(s) 289, 611
BsnI GGCC 3 cut(s) 116, 161, 367
Bso31I GGTCTC 1 cut(s) 104
BsoBI CYCGRG 1 cut(s) 22
Bsp120I GGGCCC 1 cut(s) 365
Bsp1286I GDGCHC 2 cut(s) 369, 561
Bsp143I GATC 3 cut(s) 43, 165, 580
BspACI CCGC 6 cut(s) 5, 126, 158, 198, 218, 671
BspANI GGCC 3 cut(s) 116, 161, 367
BspCNI CTCAG 1 cut(s) 267
BspFNI CGCG 1 cut(s) 399
BspHI TCATGA 1 cut(s) 685
BspLI GGNNCC 1 cut(s) 367
BspTI CTTAAG 2 cut(s) 329, 665
BspTNI GGTCTC 1 cut(s) 104
BsrBI CCGCTC 1 cut(s) 220
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 1 cut(s) 367
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 2 cut(s) 272, 432
BssMI GATC 3 cut(s) 43, 165, 580
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 1 cut(s) 432
Bst4CI ACNGT 1 cut(s) 280
Bst6I CTCTTC 1 cut(s) 581
BstACI GRCGYC 1 cut(s) 207
BstAFI CTTAAG 2 cut(s) 329, 665
BstC8I GCNNGC 3 cut(s) 156, 236, 673
BstDEI CTNAG 2 cut(s) 254, 646
BstF5I GGATG 2 cut(s) 170, 601
BstFNI CGCG 1 cut(s) 399
BstKTI GATC 3 cut(s) 46, 168, 583
BstMAI GTCTC 2 cut(s) 104, 388
BstMBI GATC 3 cut(s) 43, 165, 580
BstMCI CGRYCG 1 cut(s) 162
BstMWI GCNNNNNNNGC 4 cut(s) 11, 113, 167, 688
BstSCI CCNGG 1 cut(s) 271
BstSLI GKGCMC 1 cut(s) 369
BstUI CGCG 1 cut(s) 399
BstV1I GCAGC 2 cut(s) 346, 662
BstV2I GAAGAC 1 cut(s) 644
BstX2I RGATCY 1 cut(s) 43
BstXI CCANNNNNNTGG 1 cut(s) 95
BstYI RGATCY 1 cut(s) 43
BstZI CGGCCG 1 cut(s) 159
BsuRI GGCC 3 cut(s) 116, 161, 367
BtsCI GGATG 2 cut(s) 170, 601
Cac8I GCNNGC 3 cut(s) 156, 236, 673
CciI TCATGA 1 cut(s) 685
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 3 cut(s) 75, 365, 366
CseI GACGC 1 cut(s) 405
Csp6I GTAC 1 cut(s) 85
CviAII CATG 3 cut(s) 143, 676, 686
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 2 cut(s) 254, 646
DpnI GATC 3 cut(s) 45, 167, 582
DpnII GATC 3 cut(s) 43, 165, 580
DraI TTTAAA 1 cut(s) 373
EaeI YGGCCR 1 cut(s) 159
EagI CGGCCG 1 cut(s) 159
Eam1104I CTCTTC 1 cut(s) 581
EarI CTCTTC 1 cut(s) 581
EclXI CGGCCG 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 432
Eco24I GRGCYC 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 104
Eco47I GGWCC 1 cut(s) 75
Eco52I CGGCCG 1 cut(s) 159
Eco88I CYCGRG 1 cut(s) 22
EcoO109I RGGNCCY 1 cut(s) 366
EcoT14I CCWWGG 1 cut(s) 432
EcoT38I GRGCYC 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 432
Esp3I CGTCTC 1 cut(s) 388
FaeI CATG 3 cut(s) 146, 679, 689
FaiI YATR 7 cut(s) 90, 144, 290, 414, 643, 677, 687
FalI AAGNNNNNCTT 2 cut(s) 629, 661
FaqI GGGAC 2 cut(s) 289, 611
FatI CATG 3 cut(s) 142, 675, 685
FauI CCCGC 2 cut(s) 205, 664
FbaI TGATCA 1 cut(s) 580
Fnu4HI GCNGC 6 cut(s) 6, 127, 159, 218, 335, 651
FokI GGATG 2 cut(s) 157, 608
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 6 cut(s) 6, 127, 159, 218, 335, 651
GluI GCNGC 6 cut(s) 6, 127, 159, 218, 335, 651
HaeIII GGCC 3 cut(s) 116, 161, 367
HapII CCGG 2 cut(s) 155, 272
HgaI GACGC 1 cut(s) 405
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 3 cut(s) 146, 679, 689
HincII GTYRAC 2 cut(s) 226, 439
HindII GTYRAC 2 cut(s) 226, 439
HinfI GANTC 1 cut(s) 657
HpaII CCGG 2 cut(s) 155, 272
HphI GGTGA 2 cut(s) 463, 527
Hpy166II GTNNAC 2 cut(s) 226, 439
Hpy188I TCNGA 2 cut(s) 175, 585
Hpy188III TCNNGA 2 cut(s) 211, 686
Hpy8I GTNNAC 2 cut(s) 226, 439
HpyAV CCTTC 2 cut(s) 481, 505
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 1 cut(s) 207
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 113, 167, 688
HpyF3I CTNAG 2 cut(s) 254, 646
HpySE526I ACGT 1 cut(s) 207
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 3 cut(s) 146, 679, 689
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 3 cut(s) 43, 165, 580
LmnI GCTCC 1 cut(s) 564
Lsp1109I GCAGC 2 cut(s) 346, 662
LweI GCATC 2 cut(s) 179, 586
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 1 cut(s) 358
MalI GATC 3 cut(s) 45, 167, 582
MbiI CCGCTC 1 cut(s) 220
MboI GATC 3 cut(s) 43, 165, 580
MboII GAAGA 6 cut(s) 514, 517, 520, 583, 598, 649
MflI RGATCY 1 cut(s) 43
MhlI GDGCHC 2 cut(s) 369, 561
MluCI AATT 1 cut(s) 375
MluI ACGCGT 1 cut(s) 397
MnlI CCTC 7 cut(s) 66, 294, 511, 526, 538, 613, 693
MroNI GCCGGC 1 cut(s) 154
MseI TTAA 6 cut(s) 306, 330, 372, 378, 402, 666
MslI CAYNNNNRTG 1 cut(s) 93
MspCI CTTAAG 2 cut(s) 329, 665
MspI CCGG 2 cut(s) 155, 272
MspR9I CCNGG 1 cut(s) 273
MvnI CGCG 1 cut(s) 399
MwoI GCNNNNNNNGC 4 cut(s) 11, 113, 167, 688
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 1 cut(s) 273
NdeII GATC 3 cut(s) 43, 165, 580
NgoMIV GCCGGC 1 cut(s) 154
NlaIII CATG 3 cut(s) 146, 679, 689
NlaIV GGNNCC 1 cut(s) 367
NmeAIII GCCGAG 1 cut(s) 187
PagI TCATGA 1 cut(s) 685
PcsI WCGNNNNNNNCGW 1 cut(s) 257
PdiI GCCGGC 1 cut(s) 156
PfeI GAWTC 1 cut(s) 657
PkrI GCNGC 6 cut(s) 7, 128, 160, 219, 336, 652
PshBI ATTAAT 1 cut(s) 378
PspN4I GGNNCC 1 cut(s) 367
PspOMI GGGCCC 1 cut(s) 365
PspPI GGNCC 3 cut(s) 75, 365, 366
PsuI RGATCY 1 cut(s) 43
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
RseI CAYNNNNRTG 1 cut(s) 93
SaqAI TTAA 6 cut(s) 306, 330, 372, 378, 402, 666
SatI GCNGC 6 cut(s) 6, 127, 159, 218, 335, 651
Sau3AI GATC 3 cut(s) 43, 165, 580
Sau96I GGNCC 3 cut(s) 75, 365, 366
ScrFI CCNGG 1 cut(s) 273
SduI GDGCHC 2 cut(s) 369, 561
SetI ASST 6 cut(s) 77, 210, 305, 339, 438, 693
SfaNI GCATC 2 cut(s) 179, 586
SinI GGWCC 1 cut(s) 75
SmiMI CAYNNNNRTG 1 cut(s) 93
SmlI CTYRAG 2 cut(s) 329, 665
SmoI CTYRAG 2 cut(s) 329, 665
Sse9I AATT 1 cut(s) 375
SsiI CCGC 6 cut(s) 5, 126, 158, 198, 218, 671
SspI AATATT 1 cut(s) 479
StyD4I CCNGG 1 cut(s) 271
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 1 cut(s) 210
TaqI TCGA 2 cut(s) 71, 618
TasI AATT 1 cut(s) 375
TauI GCSGC 4 cut(s) 8, 129, 161, 220
TfiI GAWTC 1 cut(s) 657
Tru1I TTAA 6 cut(s) 306, 330, 372, 378, 402, 666
Tru9I TTAA 6 cut(s) 306, 330, 372, 378, 402, 666
TseI GCWGC 2 cut(s) 334, 650
TspDTI ATGAA 3 cut(s) 591, 623, 692
Vha464I CTTAAG 2 cut(s) 329, 665
VpaK11BI GGWCC 1 cut(s) 75
VspI ATTAAT 1 cut(s) 378
ZraI GACGTC 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.