Rroxscaffold_3G00235650

60s ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002002
Physical Location & Seq
Reverse (-)
22712328 .. 22713888
1561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00235650.1

Sequence Viewer

Length: 651 bp
ATGGCAGCAAAAGCGAGAGTTGCCAGAAAGACCCGAAACCCAGATCTGATCCGCGGTGTGGGTAAGTACTCGAGGTCCAAGATGTACCACAAGCGGGGTCTTTGGGCCATCAAGGCCAAAAACGGCGGCGTCTTCCCACGCCACGACGCCAAGCCGGCAGCTGAGGCTCCATCGGTGAAGCCCCCAAGTTCTACCCTGCCGAAGATGGCGAGTATTACACCCGGGACAGTGTTGATTATATTGGCGGGAAGATTTAAGGGAAAGAGAGTTGTTTTCCTTAAGCAACTTCCATCTGGCTTGCTTCTGGTTACTGGGCCATTTAAGATTAATGGTGTTCCTTTGAGACGTGTGAATCAGTCCTATGTGATCGGCACTTCCACCAAGGTCAACATTTCTGGGGTCAATGTGGATAAGTTTGATGATAAGTATTTCGCAAAGGCAGTGGAAAAGAAGAAGAAGAAGGGTGAGGGAGAGTTTTTTGAGGCAGAGAAAGAGGACAAGAACAAGATCCCAGAAGAAAGGAAAGATGATCAGAAGAGTGTGGATGCTTCTTTGATAAAGTCTATTGAGGCTGTCCCAGACTTGAAGACATACTTGGCTGCCAGATTCTCTCTTAGGTCCGGCATGAAGCCTCATGAGCTTGTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L6e Ribosomal_L6e_N

Protein Analysis

216

Amino Acids

23.92

Weight (kDa)

10.24

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L6e_N PF03868 4 - 61 9.2e-16 Ribosomal protein L6, N-terminal domain
Ribosomal_L6e PF01159 109 - 216 3.5e-41 Ribosomal protein L6e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 54
AciI CCGC 5 cut(s) 52, 54, 94, 126, 245
AclWI GGATC 2 cut(s) 43, 502
AcyI GRCGYC 2 cut(s) 129, 147
AfaI GTAC 2 cut(s) 68, 86
AfiI CCNNNNNNNGG 2 cut(s) 58, 94
AflII CTTAAG 1 cut(s) 278
AflIII ACRYGT 1 cut(s) 346
AgsI TTSAA 1 cut(s) 586
AjiI CACGTC 1 cut(s) 347
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
AluBI AGCT 2 cut(s) 161, 640
AluI AGCT 2 cut(s) 161, 640
Alw26I GTCTC 1 cut(s) 337
AlwI GGATC 2 cut(s) 43, 502
Ama87I CYCGRG 2 cut(s) 70, 221
AoxI GGCC 3 cut(s) 105, 114, 314
ApeKI GCWGC 3 cut(s) 5, 158, 599
AseI ATTAAT 1 cut(s) 327
AspS9I GGNCC 4 cut(s) 75, 105, 314, 618
AsuC2I CCSGG 2 cut(s) 222, 223
AsuHPI GGTGA 2 cut(s) 187, 476
AvaI CYCGRG 2 cut(s) 70, 221
AvaII GGWCC 2 cut(s) 75, 618
BbsI GAAGAC 2 cut(s) 124, 593
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 3 cut(s) 17, 170, 586
BccI CCATC 4 cut(s) 116, 178, 199, 298
BceAI ACGGC 1 cut(s) 139
BclI TGATCA 1 cut(s) 529
BcnI CCSGG 2 cut(s) 222, 223
BcoDI GTCTC 1 cut(s) 337
BfrI CTTAAG 1 cut(s) 278
BglI GCCNNNNNGGC 2 cut(s) 113, 155
BglII AGATCT 1 cut(s) 43
BisI GCNGC 4 cut(s) 6, 127, 159, 600
BlsI GCNGC 4 cut(s) 7, 128, 160, 601
BmcAI AGTACT 1 cut(s) 68
Bme1390I CCNGG 2 cut(s) 222, 223
Bme18I GGWCC 2 cut(s) 75, 618
BmeT110I CYCGRG 2 cut(s) 70, 221
BmgBI CACGTC 1 cut(s) 347
BmgT120I GGNCC 4 cut(s) 75, 105, 314, 618
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 2 cut(s) 222, 223
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 1 cut(s) 535
BmuI ACTGGG 1 cut(s) 321
BpiI GAAGAC 2 cut(s) 124, 593
Bpu10I CCTNAGC 1 cut(s) 162
BpuMI CCSGG 2 cut(s) 222, 223
BsaHI GRCGYC 2 cut(s) 129, 147
BsaJI CCNNGG 3 cut(s) 52, 221, 381
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 94
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 1 cut(s) 316
BseDI CCNNGG 3 cut(s) 52, 221, 381
BseGI GGATG 1 cut(s) 550
BseLI CCNNNNNNNGG 2 cut(s) 58, 94
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 316
BseXI GCAGC 3 cut(s) 17, 170, 586
Bsh1236I CGCG 1 cut(s) 54
BshFI GGCC 3 cut(s) 107, 116, 316
BsiHKCI CYCGRG 2 cut(s) 70, 221
BsiSI CCGG 3 cut(s) 155, 222, 621
BslFI GGGAC 2 cut(s) 238, 560
BslI CCNNNNNNNGG 2 cut(s) 58, 94
BsmAI GTCTC 1 cut(s) 337
BsmBI CGTCTC 1 cut(s) 337
BsmFI GGGAC 2 cut(s) 238, 560
BsnI GGCC 3 cut(s) 107, 116, 316
BsoBI CYCGRG 2 cut(s) 70, 221
Bsp143I GATC 5 cut(s) 43, 48, 366, 507, 529
BspACI CCGC 5 cut(s) 52, 54, 94, 126, 245
BspANI GGCC 3 cut(s) 107, 116, 316
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 1 cut(s) 54
BspHI TCATGA 1 cut(s) 634
BspLI GGNNCC 1 cut(s) 168
BspPI GGATC 2 cut(s) 43, 502
BspTI CTTAAG 1 cut(s) 278
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 1 cut(s) 316
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 3 cut(s) 52, 221, 381
BssMI GATC 5 cut(s) 43, 48, 366, 507, 529
BssNI GRCGYC 2 cut(s) 129, 147
BssT1I CCWWGG 1 cut(s) 381
Bst4CI ACNGT 1 cut(s) 229
Bst6I CTCTTC 1 cut(s) 530
BstACI GRCGYC 2 cut(s) 129, 147
BstAFI CTTAAG 1 cut(s) 278
BstC8I GCNNGC 2 cut(s) 156, 299
BstDEI CTNAG 2 cut(s) 162, 614
BstDSI CCRYGG 1 cut(s) 52
BstF5I GGATG 1 cut(s) 550
BstFNI CGCG 1 cut(s) 54
BstKTI GATC 5 cut(s) 46, 51, 369, 510, 532
BstMAI GTCTC 1 cut(s) 337
BstMBI GATC 5 cut(s) 43, 48, 366, 507, 529
BstMWI GCNNNNNNNGC 6 cut(s) 11, 20, 113, 155, 164, 637
BstSCI CCNGG 2 cut(s) 220, 221
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 3 cut(s) 17, 170, 586
BstV2I GAAGAC 2 cut(s) 124, 593
BstX2I RGATCY 2 cut(s) 43, 507
BstYI RGATCY 2 cut(s) 43, 507
BsuRI GGCC 3 cut(s) 107, 116, 316
BtgI CCRYGG 1 cut(s) 52
BtrI CACGTC 1 cut(s) 347
BtsCI GGATG 1 cut(s) 550
BtsI GCAGTG 1 cut(s) 447
BtsIMutI CAGTG 2 cut(s) 234, 447
Cac8I GCNNGC 2 cut(s) 156, 299
CciI TCATGA 1 cut(s) 634
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 4 cut(s) 75, 105, 314, 618
Cfr42I CCGCGG 1 cut(s) 55
Cfr9I CCCGGG 1 cut(s) 221
CseI GACGC 2 cut(s) 118, 155
Csp6I GTAC 2 cut(s) 67, 85
CspCI CAANNNNNGTGG 2 cut(s) 423, 458
CviAII CATG 2 cut(s) 625, 635
CviQI GTAC 2 cut(s) 67, 85
DdeI CTNAG 2 cut(s) 162, 614
DpnI GATC 5 cut(s) 45, 50, 368, 509, 531
DpnII GATC 5 cut(s) 43, 48, 366, 507, 529
Eam1104I CTCTTC 1 cut(s) 530
EarI CTCTTC 1 cut(s) 530
Eco130I CCWWGG 1 cut(s) 381
Eco47I GGWCC 2 cut(s) 75, 618
Eco88I CYCGRG 2 cut(s) 70, 221
EcoT14I CCWWGG 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 381
Esp3I CGTCTC 1 cut(s) 337
FaeI CATG 2 cut(s) 628, 638
FaiI YATR 5 cut(s) 239, 363, 592, 626, 636
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FaqI GGGAC 2 cut(s) 238, 560
FatI CATG 2 cut(s) 624, 634
FauI CCCGC 2 cut(s) 87, 238
FbaI TGATCA 1 cut(s) 529
Fnu4HI GCNGC 4 cut(s) 6, 127, 159, 600
FokI GGATG 1 cut(s) 557
Fsp4HI GCNGC 4 cut(s) 6, 127, 159, 600
GluI GCNGC 4 cut(s) 6, 127, 159, 600
HaeIII GGCC 3 cut(s) 107, 116, 316
HapII CCGG 3 cut(s) 155, 222, 621
HgaI GACGC 2 cut(s) 118, 155
Hin1I GRCGYC 2 cut(s) 129, 147
Hin1II CATG 2 cut(s) 628, 638
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 2 cut(s) 352, 606
HpaII CCGG 3 cut(s) 155, 222, 621
HphI GGTGA 2 cut(s) 187, 476
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 2 cut(s) 48, 534
Hpy188III TCNNGA 1 cut(s) 635
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 1 cut(s) 149
HpyAV CCTTC 1 cut(s) 454
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 1 cut(s) 346
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 20, 113, 155, 164, 637
HpyF3I CTNAG 2 cut(s) 162, 614
HpySE526I ACGT 1 cut(s) 346
Hsp92I GRCGYC 2 cut(s) 129, 147
Hsp92II CATG 2 cut(s) 628, 638
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
Ksp22I TGATCA 1 cut(s) 529
KspI CCGCGG 1 cut(s) 55
Kzo9I GATC 5 cut(s) 43, 48, 366, 507, 529
LmnI GCTCC 1 cut(s) 172
Lsp1109I GCAGC 3 cut(s) 17, 170, 586
LweI GCATC 1 cut(s) 535
MaeII ACGT 1 cut(s) 346
MaeIII GTNAC 1 cut(s) 307
MalI GATC 5 cut(s) 45, 50, 368, 509, 531
MboI GATC 5 cut(s) 43, 48, 366, 507, 529
MboII GAAGA 9 cut(s) 124, 214, 261, 463, 466, 469, 527, 547, 598
MflI RGATCY 2 cut(s) 43, 507
MnlI CCTC 7 cut(s) 66, 157, 460, 475, 487, 562, 642
MroNI GCCGGC 1 cut(s) 154
MseI TTAA 4 cut(s) 255, 279, 321, 327
MspA1I CMGCKG 2 cut(s) 54, 161
MspCI CTTAAG 1 cut(s) 278
MspI CCGG 3 cut(s) 155, 222, 621
MspR9I CCNGG 2 cut(s) 222, 223
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 6 cut(s) 11, 20, 113, 155, 164, 637
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 2 cut(s) 222, 223
NdeII GATC 5 cut(s) 43, 48, 366, 507, 529
NgoMIV GCCGGC 1 cut(s) 154
NlaIII CATG 2 cut(s) 628, 638
NlaIV GGNNCC 1 cut(s) 168
PaeR7I CTCGAG 1 cut(s) 70
PagI TCATGA 1 cut(s) 634
PdiI GCCGGC 1 cut(s) 156
PfeI GAWTC 2 cut(s) 352, 606
PkrI GCNGC 4 cut(s) 7, 128, 160, 601
PshBI ATTAAT 1 cut(s) 327
PspN4I GGNNCC 1 cut(s) 168
PspPI GGNCC 4 cut(s) 75, 105, 314, 618
PspXI VCTCGAGB 1 cut(s) 70
PsuI RGATCY 2 cut(s) 43, 507
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 2 cut(s) 68, 86
RsaNI GTAC 2 cut(s) 67, 85
SacII CCGCGG 1 cut(s) 55
SaqAI TTAA 4 cut(s) 255, 279, 321, 327
SatI GCNGC 4 cut(s) 6, 127, 159, 600
Sau3AI GATC 5 cut(s) 43, 48, 366, 507, 529
Sau96I GGNCC 4 cut(s) 75, 105, 314, 618
ScaI AGTACT 1 cut(s) 68
ScrFI CCNGG 2 cut(s) 222, 223
SetI ASST 6 cut(s) 77, 163, 349, 387, 620, 642
SfaNI GCATC 1 cut(s) 535
SfiI GGCCNNNNNGGCC 1 cut(s) 113
Sfr274I CTCGAG 1 cut(s) 70
Sfr303I CCGCGG 1 cut(s) 55
SgrBI CCGCGG 1 cut(s) 55
SinI GGWCC 2 cut(s) 75, 618
SlaI CTCGAG 1 cut(s) 70
SmaI CCCGGG 1 cut(s) 223
SmlI CTYRAG 2 cut(s) 70, 278
SmoI CTYRAG 2 cut(s) 70, 278
SsiI CCGC 5 cut(s) 52, 54, 94, 126, 245
StyD4I CCNGG 2 cut(s) 220, 221
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 1 cut(s) 349
TaqI TCGA 1 cut(s) 71
TatI WGTACW 1 cut(s) 66
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 2 cut(s) 352, 606
Tru1I TTAA 4 cut(s) 255, 279, 321, 327
Tru9I TTAA 4 cut(s) 255, 279, 321, 327
TscAI CASTG 2 cut(s) 234, 447
TseI GCWGC 3 cut(s) 5, 158, 599
TspDTI ATGAA 1 cut(s) 641
TspMI CCCGGG 1 cut(s) 221
TspRI CASTG 2 cut(s) 234, 447
Vha464I CTTAAG 1 cut(s) 278
VpaK11BI GGWCC 2 cut(s) 75, 618
VspI ATTAAT 1 cut(s) 327
XhoI CTCGAG 1 cut(s) 70
XmaI CCCGGG 1 cut(s) 221
ZrmI AGTACT 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.