RLG00000001959

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0002002
Physical Location & Seq
Reverse (-)
23564454 .. 23565680
1227 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001959

Sequence Viewer

Length: 621 bp
ATGTACCACAAGCGAGGTCTTTGGGCCATCAAGGCCAAAAACGGCGGCGTCTTCCCACGCCACGACGCCAAGCCGGCAGCTGAGGCTCCATCGGCGAAGCCCCCCAAGTTCTACCCTGCCGAAGATGTCAAGAAGCCCCTCGTCAACAAGCACAAGCCTAAGCCTACCAAGCTCAGGGCCAGTATTACACCCGGGACAGTGTTGATTATATTGGCCGGAAGGTTTAAGGGAAAGAGAGTTGTTTTCCTTAAGCAACTTTCATCTGGCTTGCTTCTTGTTACTGGGCCATTTAAGATTAATGGTGTTCCTTTGAGGCGTGTGAATCAGTCCTATGTGATCGGCACTTCCACCAAGGTCAACATTTCTGGGGTCAATGTGGACAAGTTTGATGACAAGTATTTCGCAAAGGCAGTGGAAAAGAAGAAGAAGAAGGGTGAGGGAGAGTTTTTTGAGGCAGAGAAAGAGAACAAGAACAAGCTCCCAGAAGAAAGGAAAGATGATCAGAAGAGTGTGGATGCTTCTTTGATAAAGTCTATTGAGGCTGTCCCAGACTTGAAGACATACTTGGCTGCCAGATTCTCTCTTAGGTCTGGCATGAAGCCTCATGAGCTTGTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L6e Ribosomal_L6e_N

Protein Analysis

207

Amino Acids

22.97

Weight (kDa)

10.09

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L6e PF01159 99 - 206 3.4e-41 Ribosomal protein L6e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 45
AcoI YGGCCR 1 cut(s) 213
AcyI GRCGYC 2 cut(s) 48, 66
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 174
AflII CTTAAG 1 cut(s) 248
AgsI TTSAA 1 cut(s) 556
AjuI GAANNNNNNNTTGG 2 cut(s) 548, 580
AluBI AGCT 4 cut(s) 80, 172, 478, 610
AluI AGCT 4 cut(s) 80, 172, 478, 610
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 5 cut(s) 24, 33, 177, 213, 284
ApeKI GCWGC 2 cut(s) 77, 569
ArsI GACNNNNNNTTYG 2 cut(s) 383, 415
AseI ATTAAT 1 cut(s) 297
AspS9I GGNCC 3 cut(s) 24, 177, 284
AsuC2I CCSGG 2 cut(s) 192, 193
AsuHPI GGTGA 1 cut(s) 446
AvaI CYCGRG 1 cut(s) 191
BbsI GAAGAC 2 cut(s) 43, 563
BbvCI CCTCAGC 1 cut(s) 81
BbvI GCAGC 2 cut(s) 89, 556
BccI CCATC 2 cut(s) 35, 97
BceAI ACGGC 1 cut(s) 58
BclI TGATCA 1 cut(s) 499
BcnI CCSGG 2 cut(s) 192, 193
BfrI CTTAAG 1 cut(s) 248
BglI GCCNNNNNGGC 2 cut(s) 32, 74
BisI GCNGC 3 cut(s) 46, 78, 570
BlsI GCNGC 3 cut(s) 47, 79, 571
Bme1390I CCNGG 2 cut(s) 192, 193
BmeT110I CYCGRG 1 cut(s) 191
BmgT120I GGNCC 3 cut(s) 24, 177, 284
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 2 cut(s) 192, 193
BmrI ACTGGG 1 cut(s) 291
BmsI GCATC 1 cut(s) 505
BmuI ACTGGG 1 cut(s) 291
BpiI GAAGAC 2 cut(s) 43, 563
Bpu10I CCTNAGC 3 cut(s) 81, 159, 173
BpuMI CCSGG 2 cut(s) 192, 193
BsaHI GRCGYC 2 cut(s) 48, 66
BsaJI CCNNGG 2 cut(s) 191, 351
Bsc4I CCNNNNNNNGG 1 cut(s) 174
Bse118I RCCGGY 1 cut(s) 73
Bse1I ACTGG 2 cut(s) 180, 286
BseDI CCNNGG 2 cut(s) 191, 351
BseGI GGATG 1 cut(s) 520
BseLI CCNNNNNNNGG 1 cut(s) 174
BseMII CTCAG 2 cut(s) 72, 187
BseNI ACTGG 2 cut(s) 180, 286
BseXI GCAGC 2 cut(s) 89, 556
BshFI GGCC 5 cut(s) 26, 35, 179, 215, 286
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 3 cut(s) 74, 192, 216
BslFI GGGAC 2 cut(s) 208, 530
BslI CCNNNNNNNGG 1 cut(s) 174
BsmFI GGGAC 2 cut(s) 208, 530
BsnI GGCC 5 cut(s) 26, 35, 179, 215, 286
BsoBI CYCGRG 1 cut(s) 191
Bsp143I GATC 2 cut(s) 336, 499
BspACI CCGC 1 cut(s) 45
BspANI GGCC 5 cut(s) 26, 35, 179, 215, 286
BspCNI CTCAG 2 cut(s) 73, 186
BspHI TCATGA 1 cut(s) 604
BspLI GGNNCC 1 cut(s) 87
BspTI CTTAAG 1 cut(s) 248
BsrFI RCCGGY 1 cut(s) 73
BsrI ACTGG 2 cut(s) 180, 286
BssAI RCCGGY 1 cut(s) 73
BssECI CCNNGG 2 cut(s) 191, 351
BssMI GATC 2 cut(s) 336, 499
BssNI GRCGYC 2 cut(s) 48, 66
BssT1I CCWWGG 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 199
Bst6I CTCTTC 1 cut(s) 500
BstACI GRCGYC 2 cut(s) 48, 66
BstAFI CTTAAG 1 cut(s) 248
BstC8I GCNNGC 2 cut(s) 75, 269
BstDEI CTNAG 4 cut(s) 81, 159, 173, 584
BstF5I GGATG 1 cut(s) 520
BstKTI GATC 2 cut(s) 339, 502
BstMBI GATC 2 cut(s) 336, 499
BstMWI GCNNNNNNNGC 6 cut(s) 32, 74, 83, 92, 169, 607
BstSCI CCNGG 2 cut(s) 190, 191
BstV1I GCAGC 2 cut(s) 89, 556
BstV2I GAAGAC 2 cut(s) 43, 563
BsuRI GGCC 5 cut(s) 26, 35, 179, 215, 286
BtsCI GGATG 1 cut(s) 520
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 2 cut(s) 204, 417
Cac8I GCNNGC 2 cut(s) 75, 269
CciI TCATGA 1 cut(s) 604
Cfr10I RCCGGY 1 cut(s) 73
Cfr13I GGNCC 3 cut(s) 24, 177, 284
Cfr9I CCCGGG 1 cut(s) 191
CseI GACGC 2 cut(s) 37, 74
Csp6I GTAC 1 cut(s) 4
CspCI CAANNNNNGTGG 2 cut(s) 393, 428
CviAII CATG 2 cut(s) 595, 605
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 4 cut(s) 81, 159, 173, 584
DpnI GATC 2 cut(s) 338, 501
DpnII GATC 2 cut(s) 336, 499
EaeI YGGCCR 1 cut(s) 213
Eam1104I CTCTTC 1 cut(s) 500
EarI CTCTTC 1 cut(s) 500
Eco130I CCWWGG 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 191
EcoT14I CCWWGG 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 2 cut(s) 598, 608
FaiI YATR 5 cut(s) 209, 333, 562, 596, 606
FalI AAGNNNNNCTT 2 cut(s) 548, 580
FaqI GGGAC 2 cut(s) 208, 530
FatI CATG 2 cut(s) 594, 604
FbaI TGATCA 1 cut(s) 499
Fnu4HI GCNGC 3 cut(s) 46, 78, 570
FokI GGATG 1 cut(s) 527
Fsp4HI GCNGC 3 cut(s) 46, 78, 570
GluI GCNGC 3 cut(s) 46, 78, 570
HaeIII GGCC 5 cut(s) 26, 35, 179, 215, 286
HapII CCGG 3 cut(s) 74, 192, 216
HgaI GACGC 2 cut(s) 37, 74
Hin1I GRCGYC 2 cut(s) 48, 66
Hin1II CATG 2 cut(s) 598, 608
HincII GTYRAC 2 cut(s) 145, 358
HindII GTYRAC 2 cut(s) 145, 358
HinfI GANTC 2 cut(s) 322, 576
HpaII CCGG 3 cut(s) 74, 192, 216
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 3 cut(s) 145, 358, 379
Hpy188I TCNGA 1 cut(s) 504
Hpy188III TCNNGA 2 cut(s) 130, 605
Hpy8I GTNNAC 3 cut(s) 145, 358, 379
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 2 cut(s) 213, 424
HpyCH4III ACNGT 1 cut(s) 199
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 74, 83, 92, 169, 607
HpyF3I CTNAG 4 cut(s) 81, 159, 173, 584
Hsp92I GRCGYC 2 cut(s) 48, 66
Hsp92II CATG 2 cut(s) 598, 608
KroI GCCGGC 1 cut(s) 73
KroNI GCCGGC 1 cut(s) 75
Ksp22I TGATCA 1 cut(s) 499
Kzo9I GATC 2 cut(s) 336, 499
LmnI GCTCC 2 cut(s) 91, 483
Lsp1109I GCAGC 2 cut(s) 89, 556
LweI GCATC 1 cut(s) 505
MaeIII GTNAC 1 cut(s) 277
MalI GATC 2 cut(s) 338, 501
MboI GATC 2 cut(s) 336, 499
MboII GAAGA 8 cut(s) 43, 134, 433, 436, 439, 497, 517, 568
MnlI CCTC 8 cut(s) 8, 76, 149, 306, 430, 445, 532, 612
MroNI GCCGGC 1 cut(s) 73
MseI TTAA 4 cut(s) 225, 249, 291, 297
MspA1I CMGCKG 1 cut(s) 80
MspCI CTTAAG 1 cut(s) 248
MspI CCGG 3 cut(s) 74, 192, 216
MspR9I CCNGG 2 cut(s) 192, 193
MwoI GCNNNNNNNGC 6 cut(s) 32, 74, 83, 92, 169, 607
NaeI GCCGGC 1 cut(s) 75
NciI CCSGG 2 cut(s) 192, 193
NdeII GATC 2 cut(s) 336, 499
NgoMIV GCCGGC 1 cut(s) 73
NlaIII CATG 2 cut(s) 598, 608
NlaIV GGNNCC 1 cut(s) 87
PagI TCATGA 1 cut(s) 604
PdiI GCCGGC 1 cut(s) 75
PfeI GAWTC 2 cut(s) 322, 576
PkrI GCNGC 3 cut(s) 47, 79, 571
PshBI ATTAAT 1 cut(s) 297
PspN4I GGNNCC 1 cut(s) 87
PspPI GGNCC 3 cut(s) 24, 177, 284
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SaqAI TTAA 4 cut(s) 225, 249, 291, 297
SatI GCNGC 3 cut(s) 46, 78, 570
Sau3AI GATC 2 cut(s) 336, 499
Sau96I GGNCC 3 cut(s) 24, 177, 284
ScrFI CCNGG 2 cut(s) 192, 193
SetI ASST 8 cut(s) 19, 82, 174, 224, 357, 480, 590, 612
SfaNI GCATC 1 cut(s) 505
SfiI GGCCNNNNNGGCC 1 cut(s) 32
SmaI CCCGGG 1 cut(s) 193
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
SsiI CCGC 1 cut(s) 45
StyD4I CCNGG 2 cut(s) 190, 191
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 1 cut(s) 199
TauI GCSGC 1 cut(s) 48
TfiI GAWTC 2 cut(s) 322, 576
Tru1I TTAA 4 cut(s) 225, 249, 291, 297
Tru9I TTAA 4 cut(s) 225, 249, 291, 297
TscAI CASTG 2 cut(s) 204, 417
TseI GCWGC 2 cut(s) 77, 569
TspDTI ATGAA 2 cut(s) 249, 611
TspMI CCCGGG 1 cut(s) 191
TspRI CASTG 2 cut(s) 204, 417
Vha464I CTTAAG 1 cut(s) 248
VspI ATTAAT 1 cut(s) 297
XmaI CCCGGG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.