Rmu_sc0002224.1_g000016

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002224.1
OG0002002
Physical Location & Seq
Forward (+)
69519 .. 70848
1330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002224.1_g000016.1.cds

Sequence Viewer

Length: 702 bp
atggcagcaaaagcgagagttgccagaaagacccgaaacccagatctgatccgcggtgtgggtaagtactcgaggtccaagatgtaccacaagcgaggtctttgggccatcaaggccaaaaacggcggcgtcttcccacgccacgacgccaagccggcagctgaggctccatctgtgaagcccccaaagttctaccctgccgaagatgtcaagaagcccctcgtcaacaagcacaagcctaagcctaccaagctcagggcgagtattacacccgggactgtgttgattatattggccggaaggtttaagggaaagagagttgttttccttaagcaactttcatctggcttgcttcttgttactgggccatttaagattaatggtgttcctttgagacgtgtgaatcagtcctatgtgatcggcacttccaccaaggtcaacatttctggggttaatgtggataagtttgatgacaagtatttcgccaaggcagtggaaaagaagaagaagaagggtgagggagagttttttgaggcagagaaagaggacaagaacaagctcccagaagaaaggaaagatgatcaaaagagtgtggatgcttctttgataaagtctattgaggctgtcccagacttgaagacatacttggctgccagattctctcttaggtctggcatgaagcctcatgagcttgtcttttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L6e Ribosomal_L6e_N

Protein Analysis

233

Amino Acids

26.01

Weight (kDa)

10.26

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 54
AciI CCGC 3 cut(s) 52, 54, 126
AclWI GGATC 1 cut(s) 43
AcoI YGGCCR 1 cut(s) 294
AcyI GRCGYC 2 cut(s) 129, 147
AfaI GTAC 2 cut(s) 68, 86
AfiI CCNNNNNNNGG 2 cut(s) 58, 255
AflII CTTAAG 1 cut(s) 329
AflIII ACRYGT 1 cut(s) 397
AgsI TTSAA 1 cut(s) 637
AjiI CACGTC 1 cut(s) 398
AjuI GAANNNNNNNTTGG 2 cut(s) 629, 661
AluBI AGCT 4 cut(s) 161, 253, 559, 691
AluI AGCT 4 cut(s) 161, 253, 559, 691
Alw26I GTCTC 1 cut(s) 388
AlwI GGATC 1 cut(s) 43
Ama87I CYCGRG 2 cut(s) 70, 272
AoxI GGCC 4 cut(s) 105, 114, 294, 365
ApeKI GCWGC 3 cut(s) 5, 158, 650
ArsI GACNNNNNNTTYG 2 cut(s) 464, 496
AseI ATTAAT 1 cut(s) 378
AspS9I GGNCC 3 cut(s) 75, 105, 365
AsuC2I CCSGG 2 cut(s) 273, 274
AsuHPI GGTGA 1 cut(s) 527
AvaI CYCGRG 2 cut(s) 70, 272
AvaII GGWCC 1 cut(s) 75
BbsI GAAGAC 2 cut(s) 124, 644
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 3 cut(s) 17, 170, 637
BccI CCATC 2 cut(s) 116, 178
BceAI ACGGC 1 cut(s) 139
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 2 cut(s) 273, 274
BcoDI GTCTC 1 cut(s) 388
BfrI CTTAAG 1 cut(s) 329
BglI GCCNNNNNGGC 2 cut(s) 113, 155
BglII AGATCT 1 cut(s) 43
BisI GCNGC 4 cut(s) 6, 127, 159, 651
BlsI GCNGC 4 cut(s) 7, 128, 160, 652
BmcAI AGTACT 1 cut(s) 68
Bme1390I CCNGG 2 cut(s) 273, 274
Bme18I GGWCC 1 cut(s) 75
BmeT110I CYCGRG 2 cut(s) 70, 272
BmgBI CACGTC 1 cut(s) 398
BmgT120I GGNCC 3 cut(s) 75, 105, 365
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 2 cut(s) 273, 274
BmrI ACTGGG 1 cut(s) 372
BmsI GCATC 1 cut(s) 586
BmuI ACTGGG 1 cut(s) 372
BpiI GAAGAC 2 cut(s) 124, 644
Bpu10I CCTNAGC 3 cut(s) 162, 240, 254
BpuMI CCSGG 2 cut(s) 273, 274
BsaHI GRCGYC 2 cut(s) 129, 147
BsaJI CCNNGG 4 cut(s) 52, 272, 432, 486
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 255
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 1 cut(s) 367
BseDI CCNNGG 4 cut(s) 52, 272, 432, 486
BseGI GGATG 1 cut(s) 601
BseLI CCNNNNNNNGG 2 cut(s) 58, 255
BseMII CTCAG 2 cut(s) 153, 268
BseNI ACTGG 1 cut(s) 367
BseXI GCAGC 3 cut(s) 17, 170, 637
Bsh1236I CGCG 1 cut(s) 54
BshFI GGCC 4 cut(s) 107, 116, 296, 367
BsiHKCI CYCGRG 2 cut(s) 70, 272
BsiSI CCGG 3 cut(s) 155, 273, 297
BslFI GGGAC 2 cut(s) 289, 611
BslI CCNNNNNNNGG 2 cut(s) 58, 255
BsmAI GTCTC 1 cut(s) 388
BsmBI CGTCTC 1 cut(s) 388
BsmFI GGGAC 2 cut(s) 289, 611
BsnI GGCC 4 cut(s) 107, 116, 296, 367
BsoBI CYCGRG 2 cut(s) 70, 272
Bsp143I GATC 4 cut(s) 43, 48, 417, 580
BspACI CCGC 3 cut(s) 52, 54, 126
BspANI GGCC 4 cut(s) 107, 116, 296, 367
BspCNI CTCAG 2 cut(s) 154, 267
BspFNI CGCG 1 cut(s) 54
BspHI TCATGA 1 cut(s) 685
BspLI GGNNCC 1 cut(s) 168
BspPI GGATC 1 cut(s) 43
BspTI CTTAAG 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 1 cut(s) 367
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 4 cut(s) 52, 272, 432, 486
BssMI GATC 4 cut(s) 43, 48, 417, 580
BssNI GRCGYC 2 cut(s) 129, 147
BssT1I CCWWGG 2 cut(s) 432, 486
Bst4CI ACNGT 1 cut(s) 280
BstACI GRCGYC 2 cut(s) 129, 147
BstAFI CTTAAG 1 cut(s) 329
BstC8I GCNNGC 2 cut(s) 156, 350
BstDEI CTNAG 4 cut(s) 162, 240, 254, 665
BstDSI CCRYGG 1 cut(s) 52
BstF5I GGATG 1 cut(s) 601
BstFNI CGCG 1 cut(s) 54
BstKTI GATC 4 cut(s) 46, 51, 420, 583
BstMAI GTCTC 1 cut(s) 388
BstMBI GATC 4 cut(s) 43, 48, 417, 580
BstMWI GCNNNNNNNGC 7 cut(s) 11, 20, 113, 155, 164, 250, 688
BstSCI CCNGG 2 cut(s) 271, 272
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 3 cut(s) 17, 170, 637
BstV2I GAAGAC 2 cut(s) 124, 644
BstX2I RGATCY 1 cut(s) 43
BstXI CCANNNNNNTGG 1 cut(s) 493
BstYI RGATCY 1 cut(s) 43
BsuRI GGCC 4 cut(s) 107, 116, 296, 367
BtgI CCRYGG 1 cut(s) 52
BtrI CACGTC 1 cut(s) 398
BtsCI GGATG 1 cut(s) 601
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 1 cut(s) 498
Cac8I GCNNGC 2 cut(s) 156, 350
CciI TCATGA 1 cut(s) 685
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 3 cut(s) 75, 105, 365
Cfr42I CCGCGG 1 cut(s) 55
Cfr9I CCCGGG 1 cut(s) 272
CseI GACGC 2 cut(s) 118, 155
Csp6I GTAC 2 cut(s) 67, 85
CviAII CATG 2 cut(s) 676, 686
CviQI GTAC 2 cut(s) 67, 85
DdeI CTNAG 4 cut(s) 162, 240, 254, 665
DpnI GATC 4 cut(s) 45, 50, 419, 582
DpnII GATC 4 cut(s) 43, 48, 417, 580
EaeI YGGCCR 1 cut(s) 294
Eco130I CCWWGG 2 cut(s) 432, 486
Eco47I GGWCC 1 cut(s) 75
Eco88I CYCGRG 2 cut(s) 70, 272
EcoT14I CCWWGG 2 cut(s) 432, 486
ErhI CCWWGG 2 cut(s) 432, 486
Esp3I CGTCTC 1 cut(s) 388
FaeI CATG 2 cut(s) 679, 689
FaiI YATR 5 cut(s) 290, 414, 643, 677, 687
FalI AAGNNNNNCTT 2 cut(s) 629, 661
FaqI GGGAC 2 cut(s) 289, 611
FatI CATG 2 cut(s) 675, 685
FbaI TGATCA 1 cut(s) 580
Fnu4HI GCNGC 4 cut(s) 6, 127, 159, 651
FokI GGATG 1 cut(s) 608
Fsp4HI GCNGC 4 cut(s) 6, 127, 159, 651
GluI GCNGC 4 cut(s) 6, 127, 159, 651
HaeIII GGCC 4 cut(s) 107, 116, 296, 367
HapII CCGG 3 cut(s) 155, 273, 297
HgaI GACGC 2 cut(s) 118, 155
Hin1I GRCGYC 2 cut(s) 129, 147
Hin1II CATG 2 cut(s) 679, 689
HincII GTYRAC 2 cut(s) 226, 439
HindII GTYRAC 2 cut(s) 226, 439
HinfI GANTC 2 cut(s) 403, 657
HpaII CCGG 3 cut(s) 155, 273, 297
HphI GGTGA 1 cut(s) 527
Hpy166II GTNNAC 2 cut(s) 226, 439
Hpy188I TCNGA 1 cut(s) 48
Hpy188III TCNNGA 2 cut(s) 211, 686
Hpy8I GTNNAC 2 cut(s) 226, 439
Hpy99I CGWCG 1 cut(s) 149
HpyAV CCTTC 2 cut(s) 294, 505
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 1 cut(s) 397
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 20, 113, 155, 164, 250, 688
HpyF3I CTNAG 4 cut(s) 162, 240, 254, 665
HpySE526I ACGT 1 cut(s) 397
Hsp92I GRCGYC 2 cut(s) 129, 147
Hsp92II CATG 2 cut(s) 679, 689
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
Ksp22I TGATCA 1 cut(s) 580
KspI CCGCGG 1 cut(s) 55
Kzo9I GATC 4 cut(s) 43, 48, 417, 580
LmnI GCTCC 2 cut(s) 172, 564
Lsp1109I GCAGC 3 cut(s) 17, 170, 637
LweI GCATC 1 cut(s) 586
MaeII ACGT 1 cut(s) 397
MaeIII GTNAC 1 cut(s) 358
MalI GATC 4 cut(s) 45, 50, 419, 582
MboI GATC 4 cut(s) 43, 48, 417, 580
MboII GAAGA 7 cut(s) 124, 215, 514, 517, 520, 578, 649
MflI RGATCY 1 cut(s) 43
MnlI CCTC 9 cut(s) 66, 89, 157, 230, 511, 526, 538, 613, 693
MroNI GCCGGC 1 cut(s) 154
MseI TTAA 5 cut(s) 306, 330, 372, 378, 453
MspA1I CMGCKG 2 cut(s) 54, 161
MspCI CTTAAG 1 cut(s) 329
MspI CCGG 3 cut(s) 155, 273, 297
MspR9I CCNGG 2 cut(s) 273, 274
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 7 cut(s) 11, 20, 113, 155, 164, 250, 688
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 2 cut(s) 273, 274
NdeII GATC 4 cut(s) 43, 48, 417, 580
NgoMIV GCCGGC 1 cut(s) 154
NlaIII CATG 2 cut(s) 679, 689
NlaIV GGNNCC 1 cut(s) 168
PaeR7I CTCGAG 1 cut(s) 70
PagI TCATGA 1 cut(s) 685
PdiI GCCGGC 1 cut(s) 156
PfeI GAWTC 2 cut(s) 403, 657
PkrI GCNGC 4 cut(s) 7, 128, 160, 652
PshBI ATTAAT 1 cut(s) 378
PspN4I GGNNCC 1 cut(s) 168
PspPI GGNCC 3 cut(s) 75, 105, 365
PspXI VCTCGAGB 1 cut(s) 70
PsuI RGATCY 1 cut(s) 43
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 2 cut(s) 68, 86
RsaNI GTAC 2 cut(s) 67, 85
SacII CCGCGG 1 cut(s) 55
SaqAI TTAA 5 cut(s) 306, 330, 372, 378, 453
SatI GCNGC 4 cut(s) 6, 127, 159, 651
Sau3AI GATC 4 cut(s) 43, 48, 417, 580
Sau96I GGNCC 3 cut(s) 75, 105, 365
ScaI AGTACT 1 cut(s) 68
ScrFI CCNGG 2 cut(s) 273, 274
SfaNI GCATC 1 cut(s) 586
SfiI GGCCNNNNNGGCC 1 cut(s) 113
Sfr274I CTCGAG 1 cut(s) 70
Sfr303I CCGCGG 1 cut(s) 55
SgrBI CCGCGG 1 cut(s) 55
SinI GGWCC 1 cut(s) 75
SlaI CTCGAG 1 cut(s) 70
SmaI CCCGGG 1 cut(s) 274
SmlI CTYRAG 2 cut(s) 70, 329
SmoI CTYRAG 2 cut(s) 70, 329
SsiI CCGC 3 cut(s) 52, 54, 126
StyD4I CCNGG 2 cut(s) 271, 272
StyI CCWWGG 2 cut(s) 432, 486
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 1 cut(s) 400
TaqI TCGA 1 cut(s) 71
TatI WGTACW 1 cut(s) 66
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 2 cut(s) 403, 657
Tru1I TTAA 5 cut(s) 306, 330, 372, 378, 453
Tru9I TTAA 5 cut(s) 306, 330, 372, 378, 453
TscAI CASTG 1 cut(s) 498
TseI GCWGC 3 cut(s) 5, 158, 650
TspDTI ATGAA 2 cut(s) 330, 692
TspMI CCCGGG 1 cut(s) 272
TspRI CASTG 1 cut(s) 498
Vha464I CTTAAG 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 75
VspI ATTAAT 1 cut(s) 378
XhoI CTCGAG 1 cut(s) 70
XmaI CCCGGG 1 cut(s) 272
ZrmI AGTACT 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.