Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0002025

Physical Location & Seq

Genomic Coordinates Forward (+)

12204369 .. 12209360

Gene Structure 4992 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2961 bp

ATGGCGACCAAGCTCTTGGCCCTATGCATACGCAACGAGAGCCGGGGCGACCTCTCACCCCGGCCCCACTACCCGTCCATGCCAAAGTTCCCCAAAGGTGTGGCGGCTGAAGAGACCAGCCTGGCAGCCGGAGCTGAGGCTAAGGCTTTGTTCTCTGTCATGGGCATGACGTGCTCCGCCTGTGCCGGGTCAGTCGAGAAGGCCGTCAAGAGGCTCCCTGGGATTCGCGAGGCTGTTGTCGACGTCTTGAACAACCGGGCGCAAGTCATGTTCTTTCCCAATTACGTTAACGCGGAGACTATTCGCGAGACGATTGAAGACGTTGGATTCCAAGCCACTTTGATTAATGATGAGGGAAATGAGAAGTCCATATTGATATGTAGAATTCGGATAAAGGGAATGACTTGCACTTCTTGCTCGACCACTGTTGAATCAGCTTTACAGGCAATTGATGGTGTACAAAAAGCCCAAGTTGCCTTAGCAACTAAAGAAGCAGATGTTCACTATGATCCAAAGATTGTGAGCTACAACCAGCTGTTGCAAACCATTGAAGACACTGGATTCGAAGGCATACTCATTACTGCAGGGGAACACATGAGCCGGATAGAGCTTGAAGTTGATGGTGTAAGGACCGATCGTTCAATGAGGATACTTGAACAGTCTCTTCAAGCACTCCCTGGTGTGCAATCTGTAGACTTTGATTCTGAAATCAAGAAAATATCTCTTTATTACAAATCATATATGACAGGGCCAAGAAGTTTCATTCATGTGATTGAAACAACCGGGTCCAGGTGTTTCAAAGCAAGGATTTTTCCAGGAGGTGAAGCAGGAAGAGATAGTCATAGAAAGGAGGAAATTAAGCAGTATTTTAGAGTCTTTCTGTGGAGTTTGGTTTTCACTATTCCGGTGTTTTTAACCTCCATGGTCTTCATGTATATTCCTGGAATTAAGCATGGCCTAGAAACTAAAATTGTGAATAATCTCATGATTGGGGAGCTCATGAGATGGATTCTGGCCACCCCGGTGCAATTCATTATAGGCCAGAGGTTCTATACTGGGGCATACAAATCACTGCGCCATGGTTCTGCCAATATGGATGTGCTAATCGCATTAGGAACAAATGCAGCCTATTTTTATTCGGTCTACTCGGTAGTGAGAGCTGCTACCTCTCCAGATTTTATGGGGACTGATTTTTTCGAGACTAGTGCCATGCTTATTTCATTCATTCTCCTTGGAAAGTACCTAGAGGTATTAACTAAGGGGAAGACATCTGATGCCATTGCCAAATTGATGGACTTGGCACCTGAAACAGCGGCATTGTTGACACTAGATGAAGAAGGAAATGTGATAAATGAACAGGAAATTGATAGTAGGTTGATACAAAAGAATGACATACTTAAAATTATTCCAGGGGCGAAAGTAGCCTCAGATGGTTATGTTACATGGGGGCAAAGCCATGTTAATGAGAGTATGATAACAGGAGAAGCACGACCAGTCGCAAAAAGAAAGGGCGACTCAGTCATTGGAGGTACTTTGAATGAGAATGGTGTCCTACATATCAAGGCAACTAGAGTTGGTTCAGAAAGTTCCCTTTCCCAGATTGTACGGCTGGTTGAGTCAGCTCAGATGGCCAAAGCTCCTGTACAGAAGTTTGCGGACCGCATTTCCAAATACTTTGTGCCACTGGTGATCCTACTTTCGTTCTTGACCTGGCTTTCCTGGTTTTTGGCTGGAAAATACCACAGCTACCCTGAATCTTGGATACCATCTTCCATGGATAGCTTTGAGCTTTCTCTCCAGTTTGGAATTTCTGTCATGGTTATAGCATGCCCTTGTGCTTTAGGCCTAGCAACTCCCACAGCTGTCATGGTTGGAACTGGAGTAGGCGCATTTCAAGGTGTATTGATCAAAGGAGGCCAAGCATTAGAAAGTGCACATAAGGTGAACTGCATTGTGTTCGACAAGACGGGGACTCTAACGATTGGAAAGCCAGTGGTTGTCAACACGCGACTTATGAAAAATATGGTGCTTCAAGAATTCTATGAACTTGTTGCTGCAGCTGAGGTGAATAGCGAACACCCGTTGGCAAAGGCCATTGTTGAGTATGCCAAAAAGTTCAGAGAAGACGAAGAGAATCCTGCTTGGCCAGGAGCAAAACACTTTGAATCCATTACTGGCCATGGGGTGAAGGCCATTGTTAGGAACAAGGAGATAATTGTTGGCAACAAGAGCTTGATAGTGGAGCACAGCATTGCGGTTCCAATTGATGCAGAAGAGATACTTGCAGAAGCGGAAGGACTCGCTCAAACTGGAATTTTAATAGCTATGGATGGACAAGTGGCTGGAGTTCTGTCCATATCTGATCCACTGAAACCAAGTGCTCGAGAAGTAATTTCCATACTCAAGTCTATGAAAGTTAGAAGCATAATGGTGACAGGTGACAACTGGGGAACTGCAAATTCTATCGCTAAGGAAGTTGGAATTCAGACCGTTATAGCAGAAGCCAAACCCGATCAGAAAGCAGAAAAAGTGAAGGACTTGCAGTCTTCAGGCTACACTGTGGCAATGGTGGGAGACGGAATCAATGACTCACCAGCACTTGTGGCAGCAGATGTAGGAATGGCAATTGGTGCAGGAACAGACATTGCCATTGAGGCAGCTGACATTGTTCTAGTGAAGAGCAACTTGGAAGATGTGATAACTGCGATTCACCTTTCCAGGAAAACCTTCACCCGTATCCGTTTGAACTACATCTGGGCTTTGGGATATAACGTTCTTGGAATTCCAATAGCTGCAGGAGCCCTTTTCCCACCTACTGGATTTCGTTTACCACCATGGATTGCTGGAGCTGCAATGGCAGCTTCTTCTGTCAGTGTTGTTTGCTGCTCTCTGCTGTTGAAGAATTATAAGAAGCCAAAGGTTCTGGACAACTTAGAGGTACGCGGAATAAGTATTGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

987

Amino Acids

107.05

Weight (kDa)

6.41

Isoelectric Point (pI)

33.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	51 - 110	6e-14	Heavy-metal-associated domain
HMA	PF00403	129 - 189	2.2e-16	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	436 - 539	1.9e-23	P-type ATPase actuator domain
Hydrolase	PF00702	649 - 871	7.2e-33	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63440
fragaria_vesca	FvH4_5g02090 FvH4_5g02090 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091
malus_domestica	MD06G1080800.v1.1 MD06G1080900.v1.1 MD14G1102600.v1.1
prunus_persica	Prupe.5G097900_v2.0.a1 Prupe.5G097900_v2.0.a1 Prupe.5G098000_v2.0.a1
pyrus_communis	pycom06g07780 pycom06g07790 pycom14g09890
rosa_chinensis	RchiOBHm_Chr7g0197781 RchiOBHm_Chr7g0198111 RchiOBHm_Chr7g0198141
rosa_laevigata	RLG00000003885 RLG00000003888 RLG00000019170
rosa_multiflora	Rmu_sc0000221.1_g000018 Rmu_sc0000221.1_g000030 Rmu_sc0006732.1_g000016
rosa_roxburghii	Rroxscaffold_3G00257940 Rroxscaffold_3G00257970 Rroxscaffold_3G00257990
rosa_rugosa	Rorug07G0045700 Rorug07G0045700 Rorug07G0045800 Rorug07G0045800 Rorug07G0045900
rosa_samantha	Rh7AG169100 Rh7AG169200 Rh7BG169900 Rh7BG172300 Rh7CG178600 Rh7CG178700 Rh7DG169200 Rh7DG171400 Rh7DG171800
rosa_wichuraiana	Rw7G014570 Rw7G014780 Rw7G014790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2907
AatII	GACGTC	1 cut(s)	246
AccB1I	GGYRCC	1 cut(s)	1300
AccB7I	CCANNNNNTGG	1 cut(s)	2816
AccI	GTMKAC	3 cut(s)	240, 693, 1143
AccII	CGCG	5 cut(s)	228, 293, 306, 2008, 2943
AciI	CCGC	9 cut(s)	104, 177, 293, 1313, 1655, 1660, 2255, 2291, 2943
AclI	AACGTT	1 cut(s)	2772
AclWI	GGATC	3 cut(s)	503, 1684, 2357
AcoI	YGGCCR	4 cut(s)	1014, 1629, 2144, 2176
AcsI	RAATTY	7 cut(s)	384, 1806, 2036, 2313, 2458, 2481, 2781
AcuI	CTGAAG	2 cut(s)	129, 2532
AcyI	GRCGYC	1 cut(s)	243
AfaI	GTAC	6 cut(s)	459, 1241, 1531, 1605, 1644, 2940
AfiI	CCNNNNNNNGG	5 cut(s)	186, 210, 789, 2199, 2816
AhdI	GACNNNNNGTC	1 cut(s)	2542
AhlI	ACTAGT	1 cut(s)	1202
AjiI	CACGTC	1 cut(s)	171
AjuI	GAANNNNNNNTTGG	2 cut(s)	2669, 2701
AleI	CACNNNNGTG	1 cut(s)	1022
AloI	GAACNNNNNNTCC	4 cut(s)	622, 654, 2757, 2789
Alw21I	GWGCWC	5 cut(s)	176, 999, 1936, 2247, 2383
Alw26I	GTCTC	6 cut(s)	107, 290, 302, 666, 1193, 2568
Alw44I	GTGCAC	1 cut(s)	1932
AlwI	GGATC	3 cut(s)	503, 1684, 2357
Ama87I	CYCGRG	1 cut(s)	2382
ApaLI	GTGCAC	1 cut(s)	1932
ApoI	RAATTY	7 cut(s)	384, 1806, 2036, 2313, 2458, 2481, 2781
ArsI	GACNNNNNNTTYG	2 cut(s)	545, 577
AseI	ATTAAT	1 cut(s)	345
Asp700I	GAANNNNTTC	1 cut(s)	2726
AspLEI	GCGC	3 cut(s)	262, 1077, 1889
AspS9I	GGNCC	6 cut(s)	19, 63, 630, 749, 786, 1657
AsuC2I	CCSGG	6 cut(s)	44, 61, 187, 257, 784, 1022
AsuII	TTCGAA	1 cut(s)	564
AvaI	CYCGRG	1 cut(s)	2382
AvaII	GGWCC	3 cut(s)	630, 786, 1657
BaeGI	GKGCMC	1 cut(s)	1936
BalI	TGGCCA	4 cut(s)	1016, 1631, 2146, 2178
BanI	GGYRCC	1 cut(s)	1300
BanII	GRGCYC	2 cut(s)	999, 2803
BbsI	GAAGAC	6 cut(s)	324, 558, 919, 1271, 2130, 2538
Bbv12I	GWGCWC	5 cut(s)	176, 999, 1936, 2247, 2383
BbvCI	CCTCAGC	2 cut(s)	135, 2061
BccI	CCATC	8 cut(s)	446, 614, 999, 1285, 1424, 1621, 1774, 2324
BceAI	ACGGC	2 cut(s)	188, 1622
BcgI	CGANNNNNNTGC	4 cut(s)	1187, 1221, 1939, 1973
BciVI	GTATCC	3 cut(s)	642, 1755, 2747
BclI	TGATCA	1 cut(s)	1905
BcnI	CCSGG	6 cut(s)	44, 61, 187, 257, 784, 1022
BcoDI	GTCTC	6 cut(s)	107, 290, 302, 666, 1193, 2568
BcuI	ACTAGT	1 cut(s)	1202
BfaI	CTAG	7 cut(s)	959, 1203, 1244, 1328, 1569, 1847, 2672
BfmI	CTRYAG	4 cut(s)	582, 690, 2055, 2793
BfuI	GTATCC	3 cut(s)	642, 1755, 2747
BglI	GCCNNNNNGGC	1 cut(s)	2654
Bme18I	GGWCC	3 cut(s)	630, 786, 1657
BmeRI	GACNNNNNGTC	1 cut(s)	2542
BmeT110I	CYCGRG	1 cut(s)	2382
BmgBI	CACGTC	1 cut(s)	171
BmgT120I	GGNCC	6 cut(s)	19, 63, 630, 749, 786, 1657
BmiI	GGNNCC	6 cut(s)	65, 215, 787, 1302, 2259, 2800
BmrI	ACTGGG	2 cut(s)	1065, 2455
BmsI	GCATC	2 cut(s)	1264, 2257
BmuI	ACTGGG	2 cut(s)	1065, 2455
BpiI	GAAGAC	6 cut(s)	324, 558, 919, 1271, 2130, 2538
BpmI	CTGGAG	5 cut(s)	1155, 1781, 1899, 2364, 2865
Bpu10I	CCTNAGC	5 cut(s)	135, 141, 478, 2061, 2469
Bpu14I	TTCGAA	1 cut(s)	564
BpuEI	CTTGAG	1 cut(s)	2387
BpuMI	CCSGG	6 cut(s)	44, 61, 187, 257, 784, 1022
BsaHI	GRCGYC	1 cut(s)	243
BsaI	GGTCTC	1 cut(s)	107
BsaWI	WCCGGW	1 cut(s)	904
Bsc4I	CCNNNNNNNGG	5 cut(s)	186, 210, 789, 2199, 2816
Bse3DI	GCAATG	5 cut(s)	1278, 2250, 2571, 2643, 2859
BseGI	GGATG	2 cut(s)	1102, 2335
BseLI	CCNNNNNNNGG	5 cut(s)	186, 210, 789, 2199, 2816
BseMI	GCAATG	5 cut(s)	1278, 2250, 2571, 2643, 2859
BseMII	CTCAG	5 cut(s)	126, 1440, 1530, 1637, 2052
BseSI	GKGCMC	1 cut(s)	1936
BsgI	GTGCAG	1 cut(s)	2652
Bsh1236I	CGCG	5 cut(s)	228, 293, 306, 2008, 2943
Bsh1285I	CGRYCG	1 cut(s)	637
BshNI	GGYRCC	1 cut(s)	1300
BsiEI	CGRYCG	1 cut(s)	637
BsiHKAI	GWGCWC	5 cut(s)	176, 999, 1936, 2247, 2383
BsiHKCI	CYCGRG	1 cut(s)	2382
BsiSI	CCGG	9 cut(s)	43, 61, 129, 186, 256, 601, 783, 905, 1022
BslFI	GGGAC	2 cut(s)	1198, 1984
BslI	CCNNNNNNNGG	5 cut(s)	186, 210, 789, 2199, 2816
BsmAI	GTCTC	6 cut(s)	107, 290, 302, 666, 1193, 2568
BsmBI	CGTCTC	2 cut(s)	302, 2568
BsmFI	GGGAC	2 cut(s)	1198, 1984
Bso31I	GGTCTC	1 cut(s)	107
BsoBI	CYCGRG	1 cut(s)	2382
Bsp119I	TTCGAA	1 cut(s)	564
Bsp1286I	GDGCHC	6 cut(s)	176, 999, 1936, 2247, 2383, 2803
Bsp1407I	TGTACA	2 cut(s)	457, 1642
Bsp143I	GATC	6 cut(s)	508, 634, 1689, 1905, 2362, 2512
Bsp19I	CCATGG	5 cut(s)	921, 1078, 1773, 2179, 2834
Bsp68I	TCGCGA	2 cut(s)	228, 306
BspACI	CCGC	9 cut(s)	104, 177, 293, 1313, 1655, 1660, 2255, 2291, 2943
BspCNI	CTCAG	5 cut(s)	127, 1439, 1529, 1636, 2053
BspFNI	CGCG	5 cut(s)	228, 293, 306, 2008, 2943
BspHI	TCATGA	2 cut(s)	984, 999
BspLI	GGNNCC	6 cut(s)	65, 215, 787, 1302, 2259, 2800
BspMAI	CTGCAG	3 cut(s)	586, 2059, 2797
BspPI	GGATC	3 cut(s)	503, 1684, 2357
BspQI	GCTCTTC	1 cut(s)	2672
BspT104I	TTCGAA	1 cut(s)	564
BspT107I	GGYRCC	1 cut(s)	1300
BspTNI	GGTCTC	1 cut(s)	107
BsrDI	GCAATG	5 cut(s)	1278, 2250, 2571, 2643, 2859
BsrGI	TGTACA	2 cut(s)	457, 1642
BssMI	GATC	6 cut(s)	508, 634, 1689, 1905, 2362, 2512
BssNI	GRCGYC	1 cut(s)	243
BssT1I	CCWWGG	6 cut(s)	921, 1078, 1231, 1773, 2179, 2834
Bst4CI	ACNGT	4 cut(s)	427, 660, 2491, 2560
Bst6I	CTCTTC	6 cut(s)	105, 669, 826, 2124, 2268, 2672
BstACI	GRCGYC	1 cut(s)	243
BstAPI	GCANNNNNTGC	3 cut(s)	171, 414, 2630
BstAUI	TGTACA	2 cut(s)	457, 1642
BstBI	TTCGAA	1 cut(s)	564
BstC8I	GCNNGC	1 cut(s)	1828
BstDSI	CCRYGG	5 cut(s)	921, 1078, 1773, 2179, 2834
BstF5I	GGATG	2 cut(s)	1102, 2335
BstFNI	CGCG	5 cut(s)	228, 293, 306, 2008, 2943
BstHHI	GCGC	3 cut(s)	262, 1077, 1889
BstKTI	GATC	6 cut(s)	511, 637, 1692, 1908, 2365, 2515
BstMAI	GTCTC	6 cut(s)	107, 290, 302, 666, 1193, 2568
BstMBI	GATC	6 cut(s)	508, 634, 1689, 1905, 2362, 2512
BstMCI	CGRYCG	1 cut(s)	637
BstNSI	RCATGY	1 cut(s)	1830
BstSFI	CTRYAG	4 cut(s)	582, 690, 2055, 2793
BstSLI	GKGCMC	1 cut(s)	1936
BstUI	CGCG	5 cut(s)	228, 293, 306, 2008, 2943
BstV2I	GAAGAC	6 cut(s)	324, 558, 919, 1271, 2130, 2538
BstXI	CCANNNNNNTGG	3 cut(s)	16, 100, 1291
BsuI	GTATCC	3 cut(s)	642, 1755, 2747
BtgI	CCRYGG	5 cut(s)	921, 1078, 1773, 2179, 2834
BtrI	CACGTC	1 cut(s)	171
BtsCI	GGATG	2 cut(s)	1102, 2335
BtsI	GCAGTG	1 cut(s)	1070
BtsIMutI	CAGTG	8 cut(s)	423, 555, 1070, 1682, 1998, 2366, 2556, 2878
BtuMI	TCGCGA	2 cut(s)	228, 306
Cac8I	GCNNGC	1 cut(s)	1828
CciI	TCATGA	2 cut(s)	984, 999
CfoI	GCGC	3 cut(s)	262, 1077, 1889
Cfr13I	GGNCC	6 cut(s)	19, 63, 630, 749, 786, 1657
CpoI	CGGWCCG	1 cut(s)	1657
Csp6I	GTAC	6 cut(s)	458, 1240, 1530, 1604, 1643, 2939
CspI	CGGWCCG	1 cut(s)	1657
CviQI	GTAC	6 cut(s)	458, 1240, 1530, 1604, 1643, 2939
DpnI	GATC	6 cut(s)	510, 636, 1691, 1907, 2364, 2514
DpnII	GATC	6 cut(s)	508, 634, 1689, 1905, 2362, 2512
DriI	GACNNNNNGTC	1 cut(s)	2542
EaeI	YGGCCR	4 cut(s)	1014, 1629, 2144, 2176
Eam1104I	CTCTTC	6 cut(s)	105, 669, 826, 2124, 2268, 2672
Eam1105I	GACNNNNNGTC	1 cut(s)	2542
EarI	CTCTTC	6 cut(s)	105, 669, 826, 2124, 2268, 2672
EciI	GGCGGA	1 cut(s)	166
Ecl136II	GAGCTC	1 cut(s)	997
Eco130I	CCWWGG	6 cut(s)	921, 1078, 1231, 1773, 2179, 2834
Eco147I	AGGCCT	1 cut(s)	1845
Eco24I	GRGCYC	2 cut(s)	999, 2803
Eco31I	GGTCTC	1 cut(s)	107
Eco47I	GGWCC	3 cut(s)	630, 786, 1657
Eco53kI	GAGCTC	1 cut(s)	997
Eco57I	CTGAAG	2 cut(s)	129, 2532
Eco88I	CYCGRG	1 cut(s)	2382
EcoICRI	GAGCTC	1 cut(s)	997
EcoRI	GAATTC	4 cut(s)	384, 2036, 2481, 2781
EcoT14I	CCWWGG	6 cut(s)	921, 1078, 1231, 1773, 2179, 2834
EcoT22I	ATGCAT	1 cut(s)	29
EcoT38I	GRGCYC	2 cut(s)	999, 2803
ErhI	CCWWGG	6 cut(s)	921, 1078, 1231, 1773, 2179, 2834
Esp3I	CGTCTC	2 cut(s)	302, 2568
FalI	AAGNNNNNCTT	4 cut(s)	2265, 2297, 2669, 2701
FaqI	GGGAC	2 cut(s)	1198, 1984
FbaI	TGATCA	1 cut(s)	1905
FblI	GTMKAC	3 cut(s)	240, 693, 1143
FokI	GGATG	2 cut(s)	1109, 2342
FriOI	GRGCYC	2 cut(s)	999, 2803
FspBI	CTAG	7 cut(s)	959, 1203, 1244, 1328, 1569, 1847, 2672
GlaI	GCGC	3 cut(s)	261, 1076, 1888
GsuI	CTGGAG	5 cut(s)	1155, 1781, 1899, 2364, 2865
HapII	CCGG	9 cut(s)	43, 61, 129, 186, 256, 601, 783, 905, 1022
HhaI	GCGC	3 cut(s)	262, 1077, 1889
Hin1I	GRCGYC	1 cut(s)	243
Hin6I	GCGC	3 cut(s)	260, 1075, 1887
HinP1I	GCGC	3 cut(s)	260, 1075, 1887
HincII	GTYRAC	4 cut(s)	241, 289, 1323, 2002
HindII	GTYRAC	4 cut(s)	241, 289, 1323, 2002
HpaI	GTTAAC	1 cut(s)	289
HpaII	CCGG	9 cut(s)	43, 61, 129, 186, 256, 601, 783, 905, 1022
Hpy99I	CGWCG	1 cut(s)	245
HpyAV	CCTTC	7 cut(s)	193, 560, 1331, 2182, 2288, 2527, 2737
HpyCH4III	ACNGT	4 cut(s)	427, 660, 2491, 2560
HpyCH4IV	ACGT	5 cut(s)	170, 243, 285, 321, 2772
HpySE526I	ACGT	5 cut(s)	170, 243, 285, 321, 2772
Hsp92I	GRCGYC	1 cut(s)	243
HspAI	GCGC	3 cut(s)	260, 1075, 1887
Ksp22I	TGATCA	1 cut(s)	1905
KspAI	GTTAAC	1 cut(s)	289
Kzo9I	GATC	6 cut(s)	508, 634, 1689, 1905, 2362, 2512
LguI	GCTCTTC	1 cut(s)	2672
LmnI	GCTCC	9 cut(s)	131, 179, 219, 994, 1642, 2150, 2242, 2798, 2846
LweI	GCATC	2 cut(s)	1264, 2257
MaeI	CTAG	7 cut(s)	959, 1203, 1244, 1328, 1569, 1847, 2672
MaeII	ACGT	5 cut(s)	170, 243, 285, 321, 2772
MaeIII	GTNAC	3 cut(s)	1438, 2431, 2438
MalI	GATC	6 cut(s)	510, 636, 1691, 1907, 2364, 2514
MboI	GATC	6 cut(s)	508, 634, 1689, 1905, 2362, 2512
MfeI	CAATTG	3 cut(s)	447, 2262, 2625
MhlI	GDGCHC	6 cut(s)	176, 999, 1936, 2247, 2383, 2803
MlsI	TGGCCA	4 cut(s)	1016, 1631, 2146, 2178
MluNI	TGGCCA	4 cut(s)	1016, 1631, 2146, 2178
MlyI	GAGTC	6 cut(s)	882, 1508, 1625, 1966, 2292, 2582
MmeI	TCCRAC	3 cut(s)	304, 1852, 2458
Mox20I	TGGCCA	4 cut(s)	1016, 1631, 2146, 2178
Mph1103I	ATGCAT	1 cut(s)	29
MroXI	GAANNNNTTC	1 cut(s)	2726
MscI	TGGCCA	4 cut(s)	1016, 1631, 2146, 2178
MseI	TTAA	9 cut(s)	288, 345, 858, 914, 948, 1253, 1398, 1461, 2318
MslI	CAYNNNNRTG	5 cut(s)	164, 767, 1022, 1461, 2429
Msp20I	TGGCCA	4 cut(s)	1016, 1631, 2146, 2178
MspA1I	CMGCKG	5 cut(s)	535, 1313, 1862, 2060, 2660
MspI	CCGG	9 cut(s)	43, 61, 129, 186, 256, 601, 783, 905, 1022
MunI	CAATTG	3 cut(s)	447, 2262, 2625
MvnI	CGCG	5 cut(s)	228, 293, 306, 2008, 2943
NciI	CCSGG	6 cut(s)	44, 61, 187, 257, 784, 1022
NcoI	CCATGG	5 cut(s)	921, 1078, 1773, 2179, 2834
NdeII	GATC	6 cut(s)	508, 634, 1689, 1905, 2362, 2512
NlaIV	GGNNCC	6 cut(s)	65, 215, 787, 1302, 2259, 2800
NmuCI	GTSAC	2 cut(s)	2431, 2438
NruI	TCGCGA	2 cut(s)	228, 306
NsiI	ATGCAT	1 cut(s)	29
NspI	RCATGY	1 cut(s)	1830
NspV	TTCGAA	1 cut(s)	564
OliI	CACNNNNGTG	1 cut(s)	1022
PaeI	GCATGC	1 cut(s)	1830
PaeR7I	CTCGAG	1 cut(s)	2382
PagI	TCATGA	2 cut(s)	984, 999
PasI	CCCWGGG	1 cut(s)	218
PceI	AGGCCT	1 cut(s)	1845
PciSI	GCTCTTC	1 cut(s)	2672
PcsI	WCGNNNNNNNCGW	1 cut(s)	201
PdmI	GAANNNNTTC	1 cut(s)	2726
PflFI	GACNNNGTC	1 cut(s)	1517
PflMI	CCANNNNNTGG	1 cut(s)	2816
PfoI	TCCNGGA	3 cut(s)	814, 940, 2717
Ple19I	CGATCG	1 cut(s)	637
PleI	GAGTC	6 cut(s)	881, 1508, 1624, 1966, 2292, 2582
PpsI	GAGTC	6 cut(s)	881, 1508, 1624, 1966, 2292, 2582
PshBI	ATTAAT	1 cut(s)	345
PsiI	TTATAA	1 cut(s)	2907
Psp124BI	GAGCTC	1 cut(s)	999
Psp1406I	AACGTT	1 cut(s)	2772
PspN4I	GGNNCC	6 cut(s)	65, 215, 787, 1302, 2259, 2800
PspPI	GGNCC	6 cut(s)	19, 63, 630, 749, 786, 1657
PstI	CTGCAG	3 cut(s)	586, 2059, 2797
PsyI	GACNNNGTC	1 cut(s)	1517
PvuI	CGATCG	1 cut(s)	637
PvuII	CAGCTG	4 cut(s)	535, 1862, 2060, 2660
RruI	TCGCGA	2 cut(s)	228, 306
RsaI	GTAC	6 cut(s)	459, 1241, 1531, 1605, 1644, 2940
RsaNI	GTAC	6 cut(s)	458, 1240, 1530, 1604, 1643, 2939
RseI	CAYNNNNRTG	5 cut(s)	164, 767, 1022, 1461, 2429
Rsr2I	CGGWCCG	1 cut(s)	1657
RsrII	CGGWCCG	1 cut(s)	1657
SacI	GAGCTC	1 cut(s)	999
SalI	GTCGAC	1 cut(s)	239
SapI	GCTCTTC	1 cut(s)	2672
SaqAI	TTAA	9 cut(s)	288, 345, 858, 914, 948, 1253, 1398, 1461, 2318
Sau3AI	GATC	6 cut(s)	508, 634, 1689, 1905, 2362, 2512
Sau96I	GGNCC	6 cut(s)	19, 63, 630, 749, 786, 1657
SchI	GAGTC	6 cut(s)	882, 1508, 1625, 1966, 2292, 2582
SduI	GDGCHC	6 cut(s)	176, 999, 1936, 2247, 2383, 2803
SfaNI	GCATC	2 cut(s)	1264, 2257
SfcI	CTRYAG	4 cut(s)	582, 690, 2055, 2793
Sfr274I	CTCGAG	1 cut(s)	2382
SfuI	TTCGAA	1 cut(s)	564
SinI	GGWCC	3 cut(s)	630, 786, 1657
SlaI	CTCGAG	1 cut(s)	2382
SmiMI	CAYNNNNRTG	5 cut(s)	164, 767, 1022, 1461, 2429
SmlI	CTYRAG	2 cut(s)	2382, 2402
SmoI	CTYRAG	2 cut(s)	2382, 2402
SpeI	ACTAGT	1 cut(s)	1202
SphI	GCATGC	1 cut(s)	1830
SseBI	AGGCCT	1 cut(s)	1845
SsiI	CCGC	9 cut(s)	104, 177, 293, 1313, 1655, 1660, 2255, 2291, 2943
SspMI	CTAG	7 cut(s)	959, 1203, 1244, 1328, 1569, 1847, 2672
SstI	GAGCTC	1 cut(s)	999
StuI	AGGCCT	1 cut(s)	1845
StyI	CCWWGG	6 cut(s)	921, 1078, 1231, 1773, 2179, 2834
TaaI	ACNGT	4 cut(s)	427, 660, 2491, 2560
TaiI	ACGT	5 cut(s)	173, 246, 288, 324, 2775
TaqI	TCGA	7 cut(s)	195, 240, 419, 564, 1197, 1959, 2383
TaqII	GACCGA	2 cut(s)	647, 1129
TatI	WGTACW	2 cut(s)	457, 1642
TauI	GCSGC	2 cut(s)	107, 1316
Tru1I	TTAA	9 cut(s)	288, 345, 858, 914, 948, 1253, 1398, 1461, 2318
Tru9I	TTAA	9 cut(s)	288, 345, 858, 914, 948, 1253, 1398, 1461, 2318
TscAI	CASTG	8 cut(s)	430, 562, 1077, 1689, 1998, 2373, 2563, 2878
TseFI	GTSAC	2 cut(s)	2431, 2438
Tsp45I	GTSAC	2 cut(s)	2431, 2438
TspGWI	ACGGA	2 cut(s)	2592, 2729
TspRI	CASTG	8 cut(s)	430, 562, 1077, 1689, 1998, 2373, 2563, 2878
Tth111I	GACNNNGTC	1 cut(s)	1517
Van91I	CCANNNNNTGG	1 cut(s)	2816
VneI	GTGCAC	1 cut(s)	1932
VpaK11BI	GGWCC	3 cut(s)	630, 786, 1657
VspI	ATTAAT	1 cut(s)	345
XapI	RAATTY	7 cut(s)	384, 1806, 2036, 2313, 2458, 2481, 2781
XceI	RCATGY	1 cut(s)	1830
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1864
XhoI	CTCGAG	1 cut(s)	2382
XmiI	GTMKAC	3 cut(s)	240, 693, 1143
XmnI	GAANNNNTTC	1 cut(s)	2726
XspI	CTAG	7 cut(s)	959, 1203, 1244, 1328, 1569, 1847, 2672
ZraI	GACGTC	1 cut(s)	244
Zsp2I	ATGCAT	1 cut(s)	29

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.