pycom14g14250

50S ribosomal protein L19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0003350
Physical Location & Seq
Forward (+)
17016826 .. 17019060
2235 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 810 bp
ATGCAATCTTTAAGTGCGAGCATCCGATTGGTCCGGAGGCAAAGAGTCGACGGCTCAAAAATTGCTCATTTCAGATCAATTGGAGCTTACCAATTGAGCGGCGGTTCTGGGTATAGCTCGTTGGCGTCGGATCGTTTGGCCTCTGCCAAGAAATTGGGGGATTTATCGGGTTTTGGGCCTTCGAAAAGTTCCTTGTTTCAGTCAGTTTCCAAGTTTTCGTATCGATCTTCGGCCCCGTTCACAACAATGAGCTCTCAAACTGCGGCCCCAACTTCTTCCTGTAATTCCAACTTATTCAAGAGTGCTCAACTAAGCTTTTTTCGATGTGCGGCAGTTTCACCTCCTTTCCCGAGAAGGTGCATTGCAACAGGGGGAAATTCTGTTCAGTCGGCTACTCAAGATTCAGGAATGTCCGTCTCTGATGTAGCTCCTCGCATCAAATTCAAGAGGCTTGATAAAACTGCCCACCACATAATGCAGATTCTAGATAAGGAAGCTTTAGAGGAAGTGAAGGTGCAGAGAGAAATACCAGATGTTAGGCCTGGTTATATGGTGCAACTCAGAGTGGAAGTACCCGACAACAAGCGACGTGTTTCAGTCGTGAAAGGTGTTGTGATTGCTAGACGTAATGCTGGTCTAAACACTACAATTAGAATCAGGAGGCAAGTGGCTGGAGTCGGGGTTGAATCTCTCTTCCCATTGTATTCGCCGAACATAAAGGAAATTAAGGTGCTGGACAAGAAGAAAGTGCGGAGGGCCAAGCTGTACTATGTCAGGGACAAAATCAATGCATTCAAGAAGCAATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L19

Protein Analysis

270

Amino Acids

29.66

Weight (kDa)

10.67

Isoelectric Point (pI)

57.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L19 PF01245 166 - 262 1.7e-28 Ribosomal protein L19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 99
AccI GTMKAC 1 cut(s) 48
AccIII TCCGGA 1 cut(s) 33
AciI CCGC 5 cut(s) 99, 102, 263, 329, 751
AclWI GGATC 1 cut(s) 138
AcsI RAATTY 2 cut(s) 376, 440
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 2 cut(s) 573, 767
AflIII ACRYGT 1 cut(s) 589
AgsI TTSAA 4 cut(s) 298, 445, 686, 796
AjiI CACGTC 1 cut(s) 590
AjnI CCWGG 1 cut(s) 541
AloI GAACNNNNNNTCC 2 cut(s) 366, 398
AluBI AGCT 7 cut(s) 86, 117, 252, 315, 428, 497, 763
AluI AGCT 7 cut(s) 86, 117, 252, 315, 428, 497, 763
Alw21I GWGCWC 2 cut(s) 254, 307
Alw26I GTCTC 1 cut(s) 421
AlwI GGATC 1 cut(s) 138
Ama87I CYCGRG 1 cut(s) 349
Aor13HI TCCGGA 1 cut(s) 33
AoxI GGCC 6 cut(s) 138, 176, 231, 264, 539, 756
ApoI RAATTY 2 cut(s) 376, 440
AspS9I GGNCC 5 cut(s) 31, 176, 232, 265, 756
AsuHPI GGTGA 1 cut(s) 330
AsuII TTCGAA 1 cut(s) 182
AvaI CYCGRG 1 cut(s) 349
AvaII GGWCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 254
Bbv12I GWGCWC 2 cut(s) 254, 307
BceAI ACGGC 1 cut(s) 67
BciT130I CCWGG 1 cut(s) 543
BcoDI GTCTC 1 cut(s) 421
BfaI CTAG 2 cut(s) 485, 621
BisI GCNGC 3 cut(s) 100, 264, 330
BlsI GCNGC 3 cut(s) 101, 265, 331
Bme1390I CCNGG 1 cut(s) 543
Bme18I GGWCC 1 cut(s) 31
BmeT110I CYCGRG 1 cut(s) 349
BmgBI CACGTC 1 cut(s) 590
BmgT120I GGNCC 5 cut(s) 31, 176, 232, 265, 756
BmiI GGNNCC 2 cut(s) 234, 267
BmrFI CCNGG 1 cut(s) 543
BmsI GCATC 2 cut(s) 30, 444
BpmI CTGGAG 1 cut(s) 693
Bpu14I TTCGAA 1 cut(s) 182
BpuEI CTTGAG 1 cut(s) 381
Bsa29I ATCGAT 1 cut(s) 223
BsaHI GRCGYC 1 cut(s) 125
BsaWI WCCGGW 1 cut(s) 33
BsaXI ACNNNNNCTCC 2 cut(s) 666, 696
Bse3DI GCAATG 1 cut(s) 360
BseAI TCCGGA 1 cut(s) 33
BseBI CCWGG 1 cut(s) 543
BseCI ATCGAT 1 cut(s) 223
BseGI GGATG 1 cut(s) 21
BseMI GCAATG 1 cut(s) 360
BseMII CTCAG 1 cut(s) 574
BseRI GAGGAG 1 cut(s) 420
BsgI GTGCAG 1 cut(s) 536
BshFI GGCC 6 cut(s) 140, 178, 233, 266, 541, 758
BshVI ATCGAT 1 cut(s) 223
BsiHKAI GWGCWC 2 cut(s) 254, 307
BsiHKCI CYCGRG 1 cut(s) 349
BsiSI CCGG 1 cut(s) 34
BslFI GGGAC 1 cut(s) 791
BsmAI GTCTC 1 cut(s) 421
BsmBI CGTCTC 1 cut(s) 421
BsmFI GGGAC 1 cut(s) 791
BsmI GAATGC 1 cut(s) 791
BsnI GGCC 6 cut(s) 140, 178, 233, 266, 541, 758
BsoBI CYCGRG 1 cut(s) 349
Bsp119I TTCGAA 1 cut(s) 182
Bsp1286I GDGCHC 2 cut(s) 254, 307
Bsp13I TCCGGA 1 cut(s) 33
Bsp143I GATC 3 cut(s) 74, 130, 224
BspACI CCGC 5 cut(s) 99, 102, 263, 329, 751
BspANI GGCC 6 cut(s) 140, 178, 233, 266, 541, 758
BspCNI CTCAG 1 cut(s) 573
BspDI ATCGAT 1 cut(s) 223
BspEI TCCGGA 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 234, 267
BspPI GGATC 1 cut(s) 138
BspT104I TTCGAA 1 cut(s) 182
BsrBI CCGCTC 1 cut(s) 99
BsrDI GCAATG 1 cut(s) 360
BssMI GATC 3 cut(s) 74, 130, 224
BssNI GRCGYC 1 cut(s) 125
Bst2UI CCWGG 1 cut(s) 543
Bst6I CTCTTC 1 cut(s) 698
BstACI GRCGYC 1 cut(s) 125
BstBI TTCGAA 1 cut(s) 182
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 2 cut(s) 311, 560
BstF5I GGATG 1 cut(s) 21
BstKTI GATC 3 cut(s) 77, 133, 227
BstMAI GTCTC 1 cut(s) 421
BstMBI GATC 3 cut(s) 74, 130, 224
BstNI CCWGG 1 cut(s) 543
BstSCI CCNGG 1 cut(s) 541
BstXI CCANNNNNNTGG 1 cut(s) 154
Bsu15I ATCGAT 1 cut(s) 223
BsuRI GGCC 6 cut(s) 140, 178, 233, 266, 541, 758
BsuTUI ATCGAT 1 cut(s) 223
BtrI CACGTC 1 cut(s) 590
BtsCI GGATG 1 cut(s) 21
Cac8I GCNNGC 1 cut(s) 19
Cfr13I GGNCC 5 cut(s) 31, 176, 232, 265, 756
ClaI ATCGAT 1 cut(s) 223
CseI GACGC 1 cut(s) 114
Csp6I GTAC 2 cut(s) 572, 766
CviQI GTAC 2 cut(s) 572, 766
DdeI CTNAG 2 cut(s) 311, 560
DpnI GATC 3 cut(s) 76, 132, 226
DpnII GATC 3 cut(s) 74, 130, 224
Eam1104I CTCTTC 1 cut(s) 698
EarI CTCTTC 1 cut(s) 698
Ecl136II GAGCTC 1 cut(s) 252
Eco147I AGGCCT 1 cut(s) 541
Eco24I GRGCYC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 252
Eco88I CYCGRG 1 cut(s) 349
EcoICRI GAGCTC 1 cut(s) 252
EcoRII CCWGG 1 cut(s) 541
EcoT22I ATGCAT 1 cut(s) 793
EcoT38I GRGCYC 1 cut(s) 254
Esp3I CGTCTC 1 cut(s) 421
FaiI YATR 6 cut(s) 114, 473, 549, 551, 716, 771
FaqI GGGAC 1 cut(s) 791
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 3 cut(s) 100, 264, 330
FokI GGATG 1 cut(s) 8
FriOI GRGCYC 1 cut(s) 254
Fsp4HI GCNGC 3 cut(s) 100, 264, 330
FspBI CTAG 2 cut(s) 485, 621
GluI GCNGC 3 cut(s) 100, 264, 330
GsuI CTGGAG 1 cut(s) 693
HaeIII GGCC 6 cut(s) 140, 178, 233, 266, 541, 758
HapII CCGG 1 cut(s) 34
HgaI GACGC 1 cut(s) 114
Hin1I GRCGYC 1 cut(s) 125
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HindIII AAGCTT 2 cut(s) 313, 495
HinfI GANTC 6 cut(s) 45, 401, 481, 654, 675, 686
HpaII CCGG 1 cut(s) 34
HphI GGTGA 1 cut(s) 330
Hpy166II GTNNAC 2 cut(s) 49, 240
Hpy188I TCNGA 5 cut(s) 26, 74, 130, 421, 563
Hpy8I GTNNAC 2 cut(s) 49, 240
Hpy99I CGWCG 3 cut(s) 53, 130, 591
HpyAV CCTTC 3 cut(s) 189, 348, 505
HpyCH4IV ACGT 2 cut(s) 589, 625
HpyCH4V TGCA 7 cut(s) 4, 360, 365, 478, 517, 556, 791
HpyF3I CTNAG 2 cut(s) 311, 560
HpySE526I ACGT 2 cut(s) 589, 625
Hsp92I GRCGYC 1 cut(s) 125
Kpn2I TCCGGA 1 cut(s) 33
Kzo9I GATC 3 cut(s) 74, 130, 224
LmnI GCTCC 2 cut(s) 83, 433
LweI GCATC 2 cut(s) 30, 444
MaeI CTAG 2 cut(s) 485, 621
MaeII ACGT 2 cut(s) 589, 625
MalI GATC 3 cut(s) 76, 132, 226
MbiI CCGCTC 1 cut(s) 99
MboI GATC 3 cut(s) 74, 130, 224
MboII GAAGA 4 cut(s) 219, 267, 685, 754
MfeI CAATTG 2 cut(s) 78, 92
MhlI GDGCHC 2 cut(s) 254, 307
MluCI AATT 9 cut(s) 60, 78, 92, 152, 283, 376, 440, 648, 723
MlyI GAGTC 2 cut(s) 54, 684
MmeI TCCRAC 2 cut(s) 108, 312
MnlI CCTC 8 cut(s) 30, 151, 351, 441, 441, 496, 654, 747
Mph1103I ATGCAT 1 cut(s) 793
MroI TCCGGA 1 cut(s) 33
MseI TTAA 2 cut(s) 11, 726
MslI CAYNNNNRTG 1 cut(s) 245
MspI CCGG 1 cut(s) 34
MspR9I CCNGG 1 cut(s) 543
MunI CAATTG 2 cut(s) 78, 92
Mva1269I GAATGC 1 cut(s) 791
MvaI CCWGG 1 cut(s) 543
NdeII GATC 3 cut(s) 74, 130, 224
NlaIV GGNNCC 2 cut(s) 234, 267
NsiI ATGCAT 1 cut(s) 793
NspV TTCGAA 1 cut(s) 182
PceI AGGCCT 1 cut(s) 541
PctI GAATGC 1 cut(s) 791
PfeI GAWTC 4 cut(s) 401, 481, 654, 686
PkrI GCNGC 3 cut(s) 101, 265, 331
PleI GAGTC 2 cut(s) 53, 683
PpsI GAGTC 2 cut(s) 53, 683
Psp124BI GAGCTC 1 cut(s) 254
Psp6I CCWGG 1 cut(s) 541
PspGI CCWGG 1 cut(s) 541
PspN4I GGNNCC 2 cut(s) 234, 267
PspPI GGNCC 5 cut(s) 31, 176, 232, 265, 756
RsaI GTAC 2 cut(s) 573, 767
RsaNI GTAC 2 cut(s) 572, 766
RseI CAYNNNNRTG 1 cut(s) 245
SacI GAGCTC 1 cut(s) 254
SalI GTCGAC 1 cut(s) 47
SaqAI TTAA 2 cut(s) 11, 726
SatI GCNGC 3 cut(s) 100, 264, 330
Sau3AI GATC 3 cut(s) 74, 130, 224
Sau96I GGNCC 5 cut(s) 31, 176, 232, 265, 756
SchI GAGTC 2 cut(s) 54, 684
ScrFI CCNGG 1 cut(s) 543
SduI GDGCHC 2 cut(s) 254, 307
SfaNI GCATC 2 cut(s) 30, 444
SfuI TTCGAA 1 cut(s) 182
SinI GGWCC 1 cut(s) 31
SmiMI CAYNNNNRTG 1 cut(s) 245
SmlI CTYRAG 1 cut(s) 396
SmoI CTYRAG 1 cut(s) 396
Sse9I AATT 9 cut(s) 60, 78, 92, 152, 283, 376, 440, 648, 723
SseBI AGGCCT 1 cut(s) 541
SsiI CCGC 5 cut(s) 99, 102, 263, 329, 751
SspMI CTAG 2 cut(s) 485, 621
SstI GAGCTC 1 cut(s) 254
StuI AGGCCT 1 cut(s) 541
StyD4I CCNGG 1 cut(s) 541
TaiI ACGT 2 cut(s) 592, 628
TaqI TCGA 4 cut(s) 48, 182, 223, 322
TasI AATT 9 cut(s) 60, 78, 92, 152, 283, 376, 440, 648, 723
TatI WGTACW 1 cut(s) 765
TauI GCSGC 3 cut(s) 102, 266, 332
TfiI GAWTC 4 cut(s) 401, 481, 654, 686
Tru1I TTAA 2 cut(s) 11, 726
Tru9I TTAA 2 cut(s) 11, 726
TspGWI ACGGA 1 cut(s) 403
VpaK11BI GGWCC 1 cut(s) 31
XapI RAATTY 2 cut(s) 376, 440
XbaI TCTAGA 1 cut(s) 484
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 2 cut(s) 485, 621
Zsp2I ATGCAT 1 cut(s) 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.