pycom14g16790

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0002194
Physical Location & Seq
Forward (+)
18953832 .. 18955606
1775 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
ATGAAAATTTCTAGGAAAATCCGTCTATTTTCCCGGAAACCCAATTCGATTTTCCCTGAAAAATTCATGGGGAGGACTGGATTGTTCGATCTGGAAAGTCACTTCGCCTTCCATGGTGCTTACCACAGCAACCGAGTTAACATTTTGATCCACACACTGTTTGTGTGGCCGATTTTCTTCACCTTTCTGGTTCTCTTCTACTTCACACCTTCTCTCTACAATTTCCCGAATGGTTTCTGTTTGAATTTAGGGTTTGCTTTCACTGTGATCTATGCTGGGTTTTATGTGTGCTTGGATAAGAAAGCTGGGTCTGTTGCTGCTCTGCTCTGTGTTCTCTGCTGGGTTCAATCCACCCTGCTCGCTAGCCGTCTTGGGTTTTCTCTGTCATGGAAGGTTGTGTTGGCTGCTCAGTTGTTCTGTTGGACTGGACAGTTCATAGGCCACGGTGTGTTCGAGAAACGAGCACCAGCTTTTTTGGACAACCGTGTTCAAGCATTTCTAATGACTCCTTTCTTTGTGCTGCTTGAGTGTCTTCAATCGGTATTCGGCTATGAACCATATCAAGGGTTTCATGCAAGTGTGGAAGAAAAGATAGAAGCTCGGATCAAGGAGTGGAGGGACAAGAAACAAAAGAAAATCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

215

Amino Acids

24.76

Weight (kDa)

9.53

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 30 - 179 1.6e-21 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 142, 611
AcoI YGGCCR 1 cut(s) 167
AcsI RAATTY 3 cut(s) 6, 62, 244
AdeI CACNNNGTG 1 cut(s) 448
AfiI CCNNNNNNNGG 1 cut(s) 563
AgsI TTSAA 4 cut(s) 244, 347, 491, 536
AjuI GAANNNNNNNTTGG 4 cut(s) 35, 67, 383, 415
AluBI AGCT 3 cut(s) 305, 470, 599
AluI AGCT 3 cut(s) 305, 470, 599
Alw21I GWGCWC 1 cut(s) 466
AlwI GGATC 2 cut(s) 142, 611
AoxI GGCC 2 cut(s) 167, 439
ApeKI GCWGC 3 cut(s) 317, 404, 520
ApoI RAATTY 3 cut(s) 6, 62, 244
Asp700I GAANNNNTTC 1 cut(s) 233
AsuC2I CCSGG 1 cut(s) 34
AsuHPI GGTGA 2 cut(s) 172, 631
AsuNHI GCTAGC 1 cut(s) 362
BbsI GAAGAC 1 cut(s) 524
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 3 cut(s) 304, 391, 507
BceAI ACGGC 1 cut(s) 351
BcnI CCSGG 1 cut(s) 34
BfaI CTAG 3 cut(s) 12, 363, 643
BisI GCNGC 3 cut(s) 318, 405, 521
BlsI GCNGC 3 cut(s) 319, 406, 522
Bme1390I CCNGG 1 cut(s) 34
BmrFI CCNGG 1 cut(s) 34
BmtI GCTAGC 1 cut(s) 366
BpiI GAAGAC 1 cut(s) 524
BpuEI CTTGAG 1 cut(s) 545
BpuMI CCSGG 1 cut(s) 34
BsaJI CCNNGG 2 cut(s) 112, 442
Bsc4I CCNNNNNNNGG 1 cut(s) 563
Bse1I ACTGG 2 cut(s) 82, 430
BseDI CCNNGG 2 cut(s) 112, 442
BseLI CCNNNNNNNGG 1 cut(s) 563
BseMII CTCAG 1 cut(s) 422
BseNI ACTGG 2 cut(s) 82, 430
BseXI GCAGC 3 cut(s) 304, 391, 507
BseYI CCCAGC 3 cut(s) 275, 305, 339
BshFI GGCC 2 cut(s) 169, 441
BsiHKAI GWGCWC 1 cut(s) 466
BsiSI CCGG 1 cut(s) 34
BslFI GGGAC 1 cut(s) 632
BslI CCNNNNNNNGG 1 cut(s) 563
BsmFI GGGAC 1 cut(s) 632
BsnI GGCC 2 cut(s) 169, 441
Bsp1286I GDGCHC 1 cut(s) 466
Bsp143I GATC 4 cut(s) 88, 147, 267, 603
Bsp19I CCATGG 1 cut(s) 112
BspANI GGCC 2 cut(s) 169, 441
BspCNI CTCAG 1 cut(s) 421
BspOI GCTAGC 1 cut(s) 366
BspPI GGATC 2 cut(s) 142, 611
BsrI ACTGG 2 cut(s) 82, 430
BssECI CCNNGG 2 cut(s) 112, 442
BssMI GATC 4 cut(s) 88, 147, 267, 603
BssT1I CCWWGG 1 cut(s) 112
Bst4CI ACNGT 5 cut(s) 159, 265, 432, 446, 485
Bst6I CTCTTC 1 cut(s) 200
BstC8I GCNNGC 2 cut(s) 360, 364
BstDEI CTNAG 1 cut(s) 408
BstDSI CCRYGG 2 cut(s) 112, 442
BstKTI GATC 4 cut(s) 91, 150, 270, 606
BstMBI GATC 4 cut(s) 88, 147, 267, 603
BstSCI CCNGG 1 cut(s) 32
BstV1I GCAGC 3 cut(s) 304, 391, 507
BstV2I GAAGAC 1 cut(s) 524
BsuRI GGCC 2 cut(s) 169, 441
BtgI CCRYGG 2 cut(s) 112, 442
BtsIMutI CAGTG 2 cut(s) 155, 261
Cac8I GCNNGC 2 cut(s) 360, 364
CviAII CATG 4 cut(s) 67, 113, 387, 572
CviJI RGCY 8 cut(s) 169, 305, 366, 404, 441, 470, 549, 599
CviKI_1 RGCY 8 cut(s) 169, 305, 366, 404, 441, 470, 549, 599
DdeI CTNAG 1 cut(s) 408
DpnI GATC 4 cut(s) 90, 149, 269, 605
DpnII GATC 4 cut(s) 88, 147, 267, 603
DraIII CACNNNGTG 1 cut(s) 448
EaeI YGGCCR 1 cut(s) 167
Eam1104I CTCTTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 112
FaeI CATG 4 cut(s) 70, 116, 390, 575
FaiI YATR 9 cut(s) 68, 114, 273, 285, 388, 437, 552, 559, 573
FaqI GGGAC 1 cut(s) 632
FatI CATG 4 cut(s) 66, 112, 386, 571
Fnu4HI GCNGC 3 cut(s) 318, 405, 521
Fsp4HI GCNGC 3 cut(s) 318, 405, 521
FspBI CTAG 3 cut(s) 12, 363, 643
GluI GCNGC 3 cut(s) 318, 405, 521
GsaI CCCAGC 3 cut(s) 279, 309, 343
HaeIII GGCC 2 cut(s) 169, 441
HapII CCGG 1 cut(s) 34
Hin1II CATG 4 cut(s) 70, 116, 390, 575
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HinfI GANTC 1 cut(s) 505
HpaI GTTAAC 1 cut(s) 139
HpaII CCGG 1 cut(s) 34
HphI GGTGA 2 cut(s) 172, 631
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 1 cut(s) 603
Hpy188III TCNNGA 3 cut(s) 92, 226, 454
Hpy8I GTNNAC 1 cut(s) 139
HpyAV CCTTC 3 cut(s) 118, 219, 385
HpyCH4III ACNGT 5 cut(s) 159, 265, 432, 446, 485
HpyCH4V TGCA 1 cut(s) 575
HpyF3I CTNAG 1 cut(s) 408
Hsp92II CATG 4 cut(s) 70, 116, 390, 575
KspAI GTTAAC 1 cut(s) 139
Kzo9I GATC 4 cut(s) 88, 147, 267, 603
Lsp1109I GCAGC 3 cut(s) 304, 391, 507
MaeI CTAG 3 cut(s) 12, 363, 643
MaeIII GTNAC 1 cut(s) 98
MalI GATC 4 cut(s) 90, 149, 269, 605
MboI GATC 4 cut(s) 88, 147, 267, 603
MboII GAAGA 4 cut(s) 169, 187, 524, 596
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 5 cut(s) 6, 43, 62, 220, 244
MlyI GAGTC 1 cut(s) 499
MmeI TCCRAC 1 cut(s) 401
MnlI CCTC 2 cut(s) 66, 609
MroXI GAANNNNTTC 1 cut(s) 233
MseI TTAA 1 cut(s) 138
MslI CAYNNNNRTG 1 cut(s) 576
MspI CCGG 1 cut(s) 34
MspR9I CCNGG 1 cut(s) 34
NciI CCSGG 1 cut(s) 34
NcoI CCATGG 1 cut(s) 112
NdeII GATC 4 cut(s) 88, 147, 267, 603
NheI GCTAGC 1 cut(s) 362
NlaIII CATG 4 cut(s) 70, 116, 390, 575
NmuCI GTSAC 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 450
PdmI GAANNNNTTC 1 cut(s) 233
PfoI TCCNGGA 1 cut(s) 32
PkrI GCNGC 3 cut(s) 319, 406, 522
PleI GAGTC 1 cut(s) 499
PpsI GAGTC 1 cut(s) 499
PspFI CCCAGC 3 cut(s) 275, 305, 339
RseI CAYNNNNRTG 1 cut(s) 576
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 3 cut(s) 318, 405, 521
Sau3AI GATC 4 cut(s) 88, 147, 267, 603
SchI GAGTC 1 cut(s) 499
ScrFI CCNGG 1 cut(s) 34
SduI GDGCHC 1 cut(s) 466
SetI ASST 7 cut(s) 185, 211, 307, 396, 472, 601, 644
SmiMI CAYNNNNRTG 1 cut(s) 576
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
Sse9I AATT 5 cut(s) 6, 43, 62, 220, 244
SspMI CTAG 3 cut(s) 12, 363, 643
StyD4I CCNGG 1 cut(s) 32
StyI CCWWGG 1 cut(s) 112
TaaI ACNGT 5 cut(s) 159, 265, 432, 446, 485
TaqI TCGA 3 cut(s) 47, 87, 453
TasI AATT 5 cut(s) 6, 43, 62, 220, 244
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TscAI CASTG 2 cut(s) 162, 268
TseFI GTSAC 1 cut(s) 98
TseI GCWGC 3 cut(s) 317, 404, 520
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 5 cut(s) 17, 55, 424, 560, 567
TspGWI ACGGA 1 cut(s) 11
TspRI CASTG 2 cut(s) 162, 268
XapI RAATTY 3 cut(s) 6, 62, 244
XmnI GAANNNNTTC 1 cut(s) 233
XspI CTAG 3 cut(s) 12, 363, 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.