pycom15g01240

Indole-3-acetic acid-induced protein ARG7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003608
Physical Location & Seq
Reverse (-)
706010 .. 706462
453 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 453 bp
ATGTCAGCCGGATTCGGAAAATGCAGCAAGATCCGCCACATTGTGCGGCTCCGCCAGTTGCTGCGGCGGTGGCGCAGCAAGGCCCGCACGTCCGCCAAGCTCATACCCTCCGATGTTCCCGCGGGACACGTGGCCGTCTGCGTGGGCACCAGCTGCACCAGATTCGTGGTGCCCGCGTCGTTCCTGAACCACCCCGTCTTCAAGAAGCTCTTGGTGCAAGCCGAGGAGGAGTACGGCTTCTCGAACAGCGGTCCGCTCGCGATCCCCTGCGACGAGTCGCAGTTCGAGGAGGTGCTTCGGTCCATTTCCCGGTCCGAGTCGGGTAACTCGACCCGGTTAGTCAACATCGCCGACTTCCAGGGATGCTGCCACGTCGGCGTCCGGAGTAACCTCGATTTGTGGGCCGATTCTCGACCGTTGCTTCGTGGACTTTCCGAGAAGACAATTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

151

Amino Acids

16.66

Weight (kDa)

9.58

Isoelectric Point (pI)

68.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 16 - 102 1.1e-27 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 146, 169
AccBSI CCGCTC 1 cut(s) 256
AccII CGCG 3 cut(s) 122, 176, 260
AccIII TCCGGA 1 cut(s) 381
AclWI GGATC 2 cut(s) 25, 256
AcoI YGGCCR 1 cut(s) 132
AcvI CACGTG 1 cut(s) 130
AcyI GRCGYC 1 cut(s) 378
AdeI CACNNNGTG 1 cut(s) 43
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 1 cut(s) 309
AflIII ACRYGT 1 cut(s) 127
AgsI TTSAA 1 cut(s) 202
AjiI CACGTC 2 cut(s) 90, 373
AjnI CCWGG 1 cut(s) 357
AluBI AGCT 3 cut(s) 100, 153, 208
AluI AGCT 3 cut(s) 100, 153, 208
AlwI GGATC 2 cut(s) 25, 256
AlwNI CAGNNNCTG 1 cut(s) 61
Aor13HI TCCGGA 1 cut(s) 381
AoxI GGCC 3 cut(s) 81, 132, 402
ApeKI GCWGC 5 cut(s) 24, 61, 75, 153, 366
ArsI GACNNNNNNTTYG 2 cut(s) 405, 437
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 5 cut(s) 82, 251, 300, 312, 402
AsuC2I CCSGG 2 cut(s) 310, 334
AvaII GGWCC 3 cut(s) 251, 300, 312
BaeGI GKGCMC 2 cut(s) 149, 174
BanI GGYRCC 2 cut(s) 146, 169
BbrPI CACGTG 1 cut(s) 130
BbsI GAAGAC 2 cut(s) 190, 446
BbvI GCAGC 5 cut(s) 36, 48, 87, 140, 353
BceAI ACGGC 2 cut(s) 119, 250
BcgI CGANNNNNNTGC 2 cut(s) 145, 179
BciT130I CCWGG 1 cut(s) 359
BcnI CCSGG 2 cut(s) 310, 334
BglI GCCNNNNNGGC 1 cut(s) 375
BisI GCNGC 7 cut(s) 25, 47, 62, 65, 76, 154, 367
BlsI GCNGC 7 cut(s) 26, 48, 63, 66, 77, 155, 368
Bme1390I CCNGG 3 cut(s) 310, 334, 359
Bme18I GGWCC 3 cut(s) 251, 300, 312
BmgBI CACGTC 2 cut(s) 90, 373
BmgT120I GGNCC 5 cut(s) 82, 251, 300, 312, 402
BmiI GGNNCC 3 cut(s) 50, 148, 171
BmrFI CCNGG 3 cut(s) 310, 334, 359
BmsI GCATC 1 cut(s) 353
BpiI GAAGAC 2 cut(s) 190, 446
BpuMI CCSGG 2 cut(s) 310, 334
BsaAI YACGTR 1 cut(s) 130
BsaHI GRCGYC 1 cut(s) 378
BsaJI CCNNGG 3 cut(s) 120, 222, 358
BsaWI WCCGGW 1 cut(s) 381
Bsc4I CCNNNNNNNGG 1 cut(s) 309
Bse1I ACTGG 1 cut(s) 55
BseAI TCCGGA 1 cut(s) 381
BseBI CCWGG 1 cut(s) 359
BseDI CCNNGG 3 cut(s) 120, 222, 358
BseGI GGATG 1 cut(s) 368
BseLI CCNNNNNNNGG 1 cut(s) 309
BseNI ACTGG 1 cut(s) 55
BseRI GAGGAG 3 cut(s) 239, 242, 302
BseSI GKGCMC 2 cut(s) 149, 174
BseXI GCAGC 5 cut(s) 36, 48, 87, 140, 353
BsgI GTGCAG 1 cut(s) 139
Bsh1236I CGCG 3 cut(s) 122, 176, 260
Bsh1285I CGRYCG 1 cut(s) 416
BshFI GGCC 3 cut(s) 83, 134, 404
BshNI GGYRCC 2 cut(s) 146, 169
BsiEI CGRYCG 1 cut(s) 416
BsiSI CCGG 4 cut(s) 9, 310, 334, 382
BslFI GGGAC 1 cut(s) 138
BslI CCNNNNNNNGG 1 cut(s) 309
BsmFI GGGAC 1 cut(s) 138
BsnI GGCC 3 cut(s) 83, 134, 404
Bsp1286I GDGCHC 2 cut(s) 149, 174
Bsp13I TCCGGA 1 cut(s) 381
Bsp143I GATC 2 cut(s) 30, 261
Bsp68I TCGCGA 1 cut(s) 260
BspANI GGCC 3 cut(s) 83, 134, 404
BspEI TCCGGA 1 cut(s) 381
BspFNI CGCG 3 cut(s) 122, 176, 260
BspLI GGNNCC 3 cut(s) 50, 148, 171
BspPI GGATC 2 cut(s) 25, 256
BspT107I GGYRCC 2 cut(s) 146, 169
BsrBI CCGCTC 1 cut(s) 256
BsrI ACTGG 1 cut(s) 55
BssECI CCNNGG 3 cut(s) 120, 222, 358
BssMI GATC 2 cut(s) 30, 261
BssNI GRCGYC 1 cut(s) 378
Bst2UI CCWGG 1 cut(s) 359
Bst4CI ACNGT 1 cut(s) 417
BstACI GRCGYC 1 cut(s) 378
BstAPI GCANNNNNTGC 1 cut(s) 153
BstBAI YACGTR 1 cut(s) 130
BstC8I GCNNGC 4 cut(s) 85, 174, 219, 258
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 1 cut(s) 368
BstFNI CGCG 3 cut(s) 122, 176, 260
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 2 cut(s) 33, 264
BstMBI GATC 2 cut(s) 30, 261
BstMCI CGRYCG 1 cut(s) 416
BstMWI GCNNNNNNNGC 6 cut(s) 33, 70, 84, 153, 214, 375
BstNI CCWGG 1 cut(s) 359
BstSCI CCNGG 3 cut(s) 308, 332, 357
BstSLI GKGCMC 2 cut(s) 149, 174
BstUI CGCG 3 cut(s) 122, 176, 260
BstV1I GCAGC 5 cut(s) 36, 48, 87, 140, 353
BstV2I GAAGAC 2 cut(s) 190, 446
BstX2I RGATCY 1 cut(s) 30
BstXI CCANNNNNNTGG 1 cut(s) 166
BstYI RGATCY 1 cut(s) 30
BsuRI GGCC 3 cut(s) 83, 134, 404
BtgI CCRYGG 1 cut(s) 120
BtgZI GCGATG 1 cut(s) 331
BtrI CACGTC 2 cut(s) 90, 373
BtsCI GGATG 1 cut(s) 368
BtuMI TCGCGA 1 cut(s) 260
Cac8I GCNNGC 4 cut(s) 85, 174, 219, 258
CaiI CAGNNNCTG 1 cut(s) 61
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 5 cut(s) 82, 251, 300, 312, 402
Cfr42I CCGCGG 1 cut(s) 123
CpoI CGGWCCG 2 cut(s) 251, 312
CseI GACGC 2 cut(s) 165, 367
Csp6I GTAC 1 cut(s) 232
CspI CGGWCCG 2 cut(s) 251, 312
CviQI GTAC 1 cut(s) 232
DpnI GATC 2 cut(s) 32, 263
DpnII GATC 2 cut(s) 30, 261
DraIII CACNNNGTG 1 cut(s) 43
EaeI YGGCCR 1 cut(s) 132
EciI GGCGGA 3 cut(s) 23, 41, 82
Eco47I GGWCC 3 cut(s) 251, 300, 312
Eco72I CACGTG 1 cut(s) 130
EcoRII CCWGG 1 cut(s) 357
FaiI YATR 1 cut(s) 104
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FaqI GGGAC 1 cut(s) 138
FauI CCCGC 4 cut(s) 92, 115, 127, 181
Fnu4HI GCNGC 7 cut(s) 25, 47, 62, 65, 76, 154, 367
FokI GGATG 1 cut(s) 375
Fsp4HI GCNGC 7 cut(s) 25, 47, 62, 65, 76, 154, 367
GlaI GCGC 1 cut(s) 74
GluI GCNGC 7 cut(s) 25, 47, 62, 65, 76, 154, 367
HaeIII GGCC 3 cut(s) 83, 134, 404
HapII CCGG 4 cut(s) 9, 310, 334, 382
HgaI GACGC 2 cut(s) 165, 367
HhaI GCGC 1 cut(s) 75
Hin1I GRCGYC 1 cut(s) 378
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 5 cut(s) 12, 162, 275, 317, 407
HpaII CCGG 4 cut(s) 9, 310, 334, 382
Hpy166II GTNNAC 2 cut(s) 343, 428
Hpy188I TCNGA 4 cut(s) 17, 112, 316, 436
Hpy188III TCNNGA 6 cut(s) 184, 202, 241, 259, 382, 411
Hpy8I GTNNAC 2 cut(s) 343, 428
Hpy99I CGWCG 3 cut(s) 181, 275, 377
HpyCH4III ACNGT 1 cut(s) 417
HpyCH4IV ACGT 3 cut(s) 89, 129, 372
HpyCH4V TGCA 3 cut(s) 24, 156, 217
HpyF10VI GCNNNNNNNGC 6 cut(s) 33, 70, 84, 153, 214, 375
HpySE526I ACGT 3 cut(s) 89, 129, 372
Hsp92I GRCGYC 1 cut(s) 378
HspAI GCGC 1 cut(s) 73
Kpn2I TCCGGA 1 cut(s) 381
KspI CCGCGG 1 cut(s) 123
Kzo9I GATC 2 cut(s) 30, 261
LmnI GCTCC 1 cut(s) 54
Lsp1109I GCAGC 5 cut(s) 36, 48, 87, 140, 353
LweI GCATC 1 cut(s) 353
MaeII ACGT 3 cut(s) 89, 129, 372
MaeIII GTNAC 2 cut(s) 323, 386
MalI GATC 2 cut(s) 32, 263
MbiI CCGCTC 1 cut(s) 256
MboI GATC 2 cut(s) 30, 261
MboII GAAGA 2 cut(s) 190, 451
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 2 cut(s) 149, 174
MluCI AATT 1 cut(s) 444
MlyI GAGTC 2 cut(s) 284, 326
MnlI CCTC 6 cut(s) 118, 217, 220, 280, 283, 401
MroI TCCGGA 1 cut(s) 381
MspA1I CMGCKG 3 cut(s) 122, 153, 249
MspI CCGG 4 cut(s) 9, 310, 334, 382
MspR9I CCNGG 3 cut(s) 310, 334, 359
MvaI CCWGG 1 cut(s) 359
MvnI CGCG 3 cut(s) 122, 176, 260
MwoI GCNNNNNNNGC 6 cut(s) 33, 70, 84, 153, 214, 375
NciI CCSGG 2 cut(s) 310, 334
NdeII GATC 2 cut(s) 30, 261
NlaIV GGNNCC 3 cut(s) 50, 148, 171
NmeAIII GCCGAG 1 cut(s) 247
NruI TCGCGA 1 cut(s) 260
PcsI WCGNNNNNNNCGW 1 cut(s) 326
PfeI GAWTC 3 cut(s) 12, 162, 407
PkrI GCNGC 7 cut(s) 26, 48, 63, 66, 77, 155, 368
PleI GAGTC 2 cut(s) 283, 325
PmaCI CACGTG 1 cut(s) 130
PmlI CACGTG 1 cut(s) 130
PpsI GAGTC 2 cut(s) 283, 325
Ppu21I YACGTR 1 cut(s) 130
Psp6I CCWGG 1 cut(s) 357
PspCI CACGTG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 357
PspN4I GGNNCC 3 cut(s) 50, 148, 171
PspPI GGNCC 5 cut(s) 82, 251, 300, 312, 402
PstNI CAGNNNCTG 1 cut(s) 61
PsuI RGATCY 1 cut(s) 30
PvuII CAGCTG 1 cut(s) 153
RruI TCGCGA 1 cut(s) 260
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
Rsr2I CGGWCCG 2 cut(s) 251, 312
RsrII CGGWCCG 2 cut(s) 251, 312
SacII CCGCGG 1 cut(s) 123
SatI GCNGC 7 cut(s) 25, 47, 62, 65, 76, 154, 367
Sau3AI GATC 2 cut(s) 30, 261
Sau96I GGNCC 5 cut(s) 82, 251, 300, 312, 402
SchI GAGTC 2 cut(s) 284, 326
ScrFI CCNGG 3 cut(s) 310, 334, 359
SduI GDGCHC 2 cut(s) 149, 174
SetI ASST 8 cut(s) 92, 102, 132, 155, 210, 294, 375, 393
SfaNI GCATC 1 cut(s) 353
Sfr303I CCGCGG 1 cut(s) 123
SgrBI CCGCGG 1 cut(s) 123
SinI GGWCC 3 cut(s) 251, 300, 312
Sse9I AATT 1 cut(s) 444
StyD4I CCNGG 3 cut(s) 308, 332, 357
TaaI ACNGT 1 cut(s) 417
TaiI ACGT 3 cut(s) 92, 132, 375
TaqI TCGA 5 cut(s) 242, 285, 329, 393, 412
TaqII GACCGA 1 cut(s) 288
TasI AATT 1 cut(s) 444
TauI GCSGC 2 cut(s) 49, 67
TfiI GAWTC 3 cut(s) 12, 162, 407
TseI GCWGC 5 cut(s) 24, 61, 75, 153, 366
VpaK11BI GGWCC 3 cut(s) 251, 300, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.