pycom15g02160

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003654
Physical Location & Seq
Reverse (-)
1272187 .. 1273953
1767 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1575 bp
ATGGCTAAATTTGTGACCCGGATGATCAGGTGCTTCTTCTCCATGGCCTTATTACTTCCAACTTTTAGGCATGCGCTATCCCAAGCAGCTCAAGTGAACATAGCCCTGGCGCCCCCAACGGTTTTTATAACCGTCGACCAGTCAGGCAGCAACGATTACACTAAAATACAAGACGCCATCGATGCTGTGCCGTCCAACAATGCCCTGCCTGTGTATATTTGGGTTAAACCTGGCATCTACTACGAAAAGATTAATGTACCTTCAGACAAACCGTTTATAACAATAAGTGGATCGACAAGTTATGCAAACGACACCGTGATAACGTGGAAGGAATCCGGCGAGATAAATGAAACCGCAGTCTTCACTTTATGGGCATCTGATTTCGTAGGGCGCAACTTCAAAGTTCAGAATACGTATGGGACGGGTGGCAAAGCAGTTGCACTGAGAGTTTCGGGAGATAGAGTAGCATTCTATGGTTGCAGCATTATGTCTCATCAAGATGCCTTATTTGCCGATATAGGCAAGCAATACTACAAAGACTGCTACATTGAAGGAGACACTGATTTTATATTTGGAAGAGCTTCTTCTTTATTTGAGAATTGTCACATGCATTCGCTTTCGGAACAAAACGGAGCTATAACAGCCCAAAAAAGGGATTCACCATCACAAGATGCAGGGTTTGTGTTCTTGGGAGGCAAAATCACTGGCGTCGGAAGGGCAGTACTAGGAAGGCCATGGGGTCCCTATTCGACAGTCATCTTTGCCTATACTAAGATGTCGGATGCGATTCTCCCACAAGGTTGGGACAGTTGGGGCCGATCAACTGAGTACTTAAGTAAGGTTTACTATGGTGAGTACAAATGTACTGGAGCTGGAGCCGTGACAGCAGAAAGAGTTGCGTGGGAACACAACGTTACGCCAAAAGAATTTACATCCTTCATGAACAAGGTTAGGGGCTTTGGAATTGGAAGCTGGCAAGATTCCGATGGAGGAGGATGGAAATATGGAGCTCCCCCTCGTGTCAGAAGAGATGGAGAAGGAAACAATGGAGGACGGCAACAAGGACCTCCTACATCTATTGGAGATGGAGGGAAACAAGGATCTCCTACTCCCATTGGAGGTGGAGGGCAACATGGACCTCCTCCTCCCGCAACTAGAGAAGGAGGACAATATGGAGCTCCCGCTTCCTTTGCAGGAGGATGGCTACATGGACCTCCTCCTTCCATTGCAGGAGGAGGACAATATGGAGCTCCCCCTCCCTTTGCAGGAGGATGGCAAAAAGGACCTCCTACTCCGGTTGGGGGAGGAGGGCAACATAGAGCACCTCCTCCTGCTGCCGGAGGAGAACGAGAAAGAAACAATGGAGGAGGATGGAAACATGCAGCACCCATTCCCATTGGAGGAGGAGGTGGAAGAAACAATGCAGGATTAGGGCAACATGGAGTTCCTACTGTAGGAGGAGCCTCCATCGAGGGGTGGGCTGATGTGCAATGGAGTGGTTGTGAAAAAGGTGCTGCCCGTCCAGCCCTTTTGGGATGGTTTCTTGTTTTGCTTCCTTTAATCCACTTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

525

Amino Acids

55.46

Weight (kDa)

8.71

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 43 - 303 4.3e-62 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 128, 278
AccB1I GGYRCC 1 cut(s) 109
AccI GTMKAC 1 cut(s) 135
AciI CCGC 3 cut(s) 354, 1149, 1182
AclI AACGTT 1 cut(s) 912
AclWI GGATC 2 cut(s) 298, 1108
AcsI RAATTY 2 cut(s) 8, 926
AcuI CTGAAG 1 cut(s) 246
AcyI GRCGYC 3 cut(s) 110, 174, 708
AfaI GTAC 5 cut(s) 258, 723, 830, 857, 865
AfiI CCNNNNNNNGG 9 cut(s) 651, 652, 1118, 1265, 1301, 1337, 1400, 1454, 1473
AflII CTTAAG 1 cut(s) 832
AgsI TTSAA 2 cut(s) 400, 551
AjnI CCWGG 2 cut(s) 105, 229
AluBI AGCT 8 cut(s) 89, 581, 635, 872, 972, 1010, 1178, 1250
AluI AGCT 8 cut(s) 89, 581, 635, 872, 972, 1010, 1178, 1250
Alw21I GWGCWC 4 cut(s) 1012, 1180, 1252, 1324
Alw26I GTCTC 2 cut(s) 495, 549
AlwI GGATC 2 cut(s) 298, 1108
AoxI GGCC 3 cut(s) 45, 731, 814
ApeKI GCWGC 6 cut(s) 86, 147, 480, 1334, 1382, 1514
ApoI RAATTY 2 cut(s) 8, 926
AseI ATTAAT 1 cut(s) 252
Asp700I GAANNNNTTC 1 cut(s) 580
AspLEI GCGC 3 cut(s) 76, 112, 393
AspS9I GGNCC 6 cut(s) 740, 814, 1064, 1136, 1211, 1283
AsuC2I CCSGG 1 cut(s) 19
AsuHPI GGTGA 2 cut(s) 651, 863
AvaII GGWCC 5 cut(s) 740, 1064, 1136, 1211, 1283
BanI GGYRCC 1 cut(s) 109
BanII GRGCYC 3 cut(s) 1012, 1180, 1252
BauI CACGAG 1 cut(s) 1017
BbsI GAAGAC 1 cut(s) 352
Bbv12I GWGCWC 4 cut(s) 1012, 1180, 1252, 1324
BbvI GCAGC 6 cut(s) 98, 159, 492, 1321, 1394, 1501
BceAI ACGGC 3 cut(s) 175, 863, 1070
BciT130I CCWGG 2 cut(s) 107, 231
BclI TGATCA 1 cut(s) 24
BcnI CCSGG 1 cut(s) 19
BcoDI GTCTC 2 cut(s) 495, 549
BfaI CTAG 2 cut(s) 725, 1155
BfmI CTRYAG 1 cut(s) 1452
BfoI RGCGCY 1 cut(s) 113
BfrI CTTAAG 1 cut(s) 832
BisI GCNGC 6 cut(s) 87, 148, 481, 1335, 1383, 1515
BlsI GCNGC 6 cut(s) 88, 149, 482, 1336, 1384, 1516
BmcAI AGTACT 2 cut(s) 723, 830
Bme1390I CCNGG 3 cut(s) 19, 107, 231
Bme18I GGWCC 5 cut(s) 740, 1064, 1136, 1211, 1283
BmgT120I GGNCC 6 cut(s) 740, 814, 1064, 1136, 1211, 1283
BmiI GGNNCC 6 cut(s) 111, 741, 742, 815, 877, 1462
BmrFI CCNGG 3 cut(s) 19, 107, 231
BmsI GCATC 6 cut(s) 172, 243, 383, 490, 661, 772
BpiI GAAGAC 1 cut(s) 352
BpmI CTGGAG 2 cut(s) 888, 894
BpuEI CTTGAG 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 19
Bsa29I ATCGAT 1 cut(s) 180
BsaAI YACGTR 1 cut(s) 414
BsaHI GRCGYC 3 cut(s) 110, 174, 708
BsaJI CCNNGG 3 cut(s) 42, 105, 734
BsaWI WCCGGW 1 cut(s) 1294
BsaXI ACNNNNNCTCC 4 cut(s) 447, 477, 1486, 1516
Bsc4I CCNNNNNNNGG 9 cut(s) 651, 652, 1118, 1265, 1301, 1337, 1400, 1454, 1473
Bse1I ACTGG 3 cut(s) 139, 709, 871
Bse3DI GCAATG 2 cut(s) 1224, 1496
BseBI CCWGG 2 cut(s) 107, 231
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 3 cut(s) 42, 105, 734
BseGI GGATG 8 cut(s) 27, 787, 932, 1001, 1205, 1277, 1376, 1541
BseLI CCNNNNNNNGG 9 cut(s) 651, 652, 1118, 1265, 1301, 1337, 1400, 1454, 1473
BseMI GCAATG 2 cut(s) 1224, 1496
BseMII CTCAG 2 cut(s) 434, 816
BseNI ACTGG 3 cut(s) 139, 709, 871
BseXI GCAGC 6 cut(s) 98, 159, 492, 1321, 1394, 1501
BshFI GGCC 3 cut(s) 47, 733, 816
BshNI GGYRCC 1 cut(s) 109
BshVI ATCGAT 1 cut(s) 180
BsiHKAI GWGCWC 4 cut(s) 1012, 1180, 1252, 1324
BsiSI CCGG 4 cut(s) 19, 336, 1295, 1338
BslFI GGGAC 3 cut(s) 433, 726, 818
BslI CCNNNNNNNGG 9 cut(s) 651, 652, 1118, 1265, 1301, 1337, 1400, 1454, 1473
BsmAI GTCTC 2 cut(s) 495, 549
BsmFI GGGAC 3 cut(s) 433, 726, 818
BsmI GAATGC 2 cut(s) 467, 610
BsnI GGCC 3 cut(s) 47, 733, 816
Bsp1286I GDGCHC 4 cut(s) 1012, 1180, 1252, 1324
Bsp143I GATC 4 cut(s) 24, 290, 818, 1100
Bsp19I CCATGG 2 cut(s) 42, 734
BspACI CCGC 3 cut(s) 354, 1149, 1182
BspANI GGCC 3 cut(s) 47, 733, 816
BspCNI CTCAG 2 cut(s) 435, 817
BspDI ATCGAT 1 cut(s) 180
BspHI TCATGA 1 cut(s) 939
BspLI GGNNCC 6 cut(s) 111, 741, 742, 815, 877, 1462
BspPI GGATC 2 cut(s) 298, 1108
BspQI GCTCTTC 1 cut(s) 571
BspT107I GGYRCC 1 cut(s) 109
BspTI CTTAAG 1 cut(s) 832
BsrDI GCAATG 2 cut(s) 1224, 1496
BsrI ACTGG 3 cut(s) 139, 709, 871
BssECI CCNNGG 3 cut(s) 42, 105, 734
BssMI GATC 4 cut(s) 24, 290, 818, 1100
BssNI GRCGYC 3 cut(s) 110, 174, 708
BssSI CACGAG 1 cut(s) 1017
BssT1I CCWWGG 2 cut(s) 42, 734
Bst2BI CACGAG 1 cut(s) 1017
Bst2UI CCWGG 2 cut(s) 107, 231
Bst4CI ACNGT 7 cut(s) 121, 133, 273, 316, 754, 809, 1453
Bst6I CTCTTC 2 cut(s) 571, 1021
BstACI GRCGYC 3 cut(s) 110, 174, 708
BstAFI CTTAAG 1 cut(s) 832
BstBAI YACGTR 1 cut(s) 414
BstC8I GCNNGC 3 cut(s) 72, 524, 974
BstDEI CTNAG 3 cut(s) 443, 771, 825
BstDSI CCRYGG 2 cut(s) 42, 734
BstENI CCTNNNNNAGG 1 cut(s) 1452
BstF5I GGATG 8 cut(s) 27, 787, 932, 1001, 1205, 1277, 1376, 1541
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 3 cut(s) 76, 112, 393
BstKTI GATC 4 cut(s) 27, 293, 821, 1103
BstMAI GTCTC 2 cut(s) 495, 549
BstMBI GATC 4 cut(s) 24, 290, 818, 1100
BstMWI GCNNNNNNNGC 6 cut(s) 182, 509, 641, 884, 1190, 1523
BstNI CCWGG 2 cut(s) 107, 231
BstNSI RCATGY 3 cut(s) 74, 610, 1382
BstSCI CCNGG 3 cut(s) 17, 105, 229
BstSFI CTRYAG 1 cut(s) 1452
BstSNI TACGTA 1 cut(s) 414
BstV1I GCAGC 6 cut(s) 98, 159, 492, 1321, 1394, 1501
BstV2I GAAGAC 1 cut(s) 352
BstX2I RGATCY 1 cut(s) 1100
BstXI CCANNNNNNTGG 1 cut(s) 801
BstYI RGATCY 1 cut(s) 1100
Bsu15I ATCGAT 1 cut(s) 180
BsuRI GGCC 3 cut(s) 47, 733, 816
BsuTUI ATCGAT 1 cut(s) 180
BtgI CCRYGG 2 cut(s) 42, 734
BtsCI GGATG 8 cut(s) 27, 787, 932, 1001, 1205, 1277, 1376, 1541
BtsIMutI CAGTG 3 cut(s) 440, 558, 702
Cac8I GCNNGC 3 cut(s) 72, 524, 974
CciI TCATGA 1 cut(s) 939
CfoI GCGC 3 cut(s) 76, 112, 393
Cfr13I GGNCC 6 cut(s) 740, 814, 1064, 1136, 1211, 1283
ClaI ATCGAT 1 cut(s) 180
CseI GACGC 2 cut(s) 182, 697
Csp6I GTAC 5 cut(s) 257, 722, 829, 856, 864
CviAII CATG 9 cut(s) 43, 71, 607, 735, 940, 1133, 1208, 1379, 1439
CviQI GTAC 5 cut(s) 257, 722, 829, 856, 864
DdeI CTNAG 3 cut(s) 443, 771, 825
DinI GGCGCC 1 cut(s) 111
DpnI GATC 4 cut(s) 26, 292, 820, 1102
DpnII GATC 4 cut(s) 24, 290, 818, 1100
Eam1104I CTCTTC 2 cut(s) 571, 1021
EarI CTCTTC 2 cut(s) 571, 1021
Ecl136II GAGCTC 3 cut(s) 1010, 1178, 1250
Eco105I TACGTA 1 cut(s) 414
Eco130I CCWWGG 2 cut(s) 42, 734
Eco24I GRGCYC 3 cut(s) 1012, 1180, 1252
Eco47I GGWCC 5 cut(s) 740, 1064, 1136, 1211, 1283
Eco53kI GAGCTC 3 cut(s) 1010, 1178, 1250
Eco57I CTGAAG 1 cut(s) 246
EcoICRI GAGCTC 3 cut(s) 1010, 1178, 1250
EcoNI CCTNNNNNAGG 1 cut(s) 1452
EcoO109I RGGNCCY 3 cut(s) 740, 1064, 1283
EcoRII CCWGG 2 cut(s) 105, 229
EcoT14I CCWWGG 2 cut(s) 42, 734
EcoT22I ATGCAT 1 cut(s) 612
EcoT38I GRGCYC 3 cut(s) 1012, 1180, 1252
EgeI GGCGCC 1 cut(s) 111
EheI GGCGCC 1 cut(s) 111
ErhI CCWWGG 2 cut(s) 42, 734
FaeI CATG 9 cut(s) 46, 74, 610, 738, 943, 1136, 1211, 1382, 1442
FalI AAGNNNNNCTT 2 cut(s) 568, 600
FaqI GGGAC 3 cut(s) 433, 726, 818
FatI CATG 9 cut(s) 42, 70, 606, 734, 939, 1132, 1207, 1378, 1438
FauI CCCGC 2 cut(s) 1156, 1189
FbaI TGATCA 1 cut(s) 24
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 6 cut(s) 87, 148, 481, 1335, 1383, 1515
FokI GGATG 8 cut(s) 34, 794, 919, 1008, 1212, 1284, 1383, 1548
FriOI GRGCYC 3 cut(s) 1012, 1180, 1252
Fsp4HI GCNGC 6 cut(s) 87, 148, 481, 1335, 1383, 1515
FspBI CTAG 2 cut(s) 725, 1155
GlaI GCGC 3 cut(s) 75, 111, 392
GluI GCNGC 6 cut(s) 87, 148, 481, 1335, 1383, 1515
GsuI CTGGAG 2 cut(s) 888, 894
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 3 cut(s) 47, 733, 816
HapII CCGG 4 cut(s) 19, 336, 1295, 1338
HgaI GACGC 2 cut(s) 182, 697
HhaI GCGC 3 cut(s) 76, 112, 393
Hin1I GRCGYC 3 cut(s) 110, 174, 708
Hin1II CATG 9 cut(s) 46, 74, 610, 738, 943, 1136, 1211, 1382, 1442
Hin6I GCGC 3 cut(s) 74, 110, 391
HinP1I GCGC 3 cut(s) 74, 110, 391
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 4 cut(s) 332, 656, 787, 980
HpaII CCGG 4 cut(s) 19, 336, 1295, 1338
HphI GGTGA 2 cut(s) 651, 863
Hpy166II GTNNAC 3 cut(s) 97, 136, 844
Hpy188I TCNGA 8 cut(s) 265, 379, 408, 622, 713, 781, 985, 1025
Hpy188III TCNNGA 3 cut(s) 453, 497, 940
Hpy8I GTNNAC 3 cut(s) 97, 136, 844
Hpy99I CGWCG 2 cut(s) 137, 713
HpyAV CCTTC 9 cut(s) 270, 322, 545, 708, 723, 946, 1031, 1154, 1230
HpyCH4III ACNGT 7 cut(s) 121, 133, 273, 316, 754, 809, 1453
HpyCH4IV ACGT 3 cut(s) 323, 413, 912
HpyF10VI GCNNNNNNNGC 6 cut(s) 182, 509, 641, 884, 1190, 1523
HpyF3I CTNAG 3 cut(s) 443, 771, 825
HpySE526I ACGT 3 cut(s) 323, 413, 912
Hsp92I GRCGYC 3 cut(s) 110, 174, 708
Hsp92II CATG 9 cut(s) 46, 74, 610, 738, 943, 1136, 1211, 1382, 1442
HspAI GCGC 3 cut(s) 74, 110, 391
KasI GGCGCC 1 cut(s) 109
KflI GGGWCCC 1 cut(s) 740
Ksp22I TGATCA 1 cut(s) 24
Kzo9I GATC 4 cut(s) 24, 290, 818, 1100
LguI GCTCTTC 1 cut(s) 571
Lsp1109I GCAGC 6 cut(s) 98, 159, 492, 1321, 1394, 1501
LweI GCATC 6 cut(s) 172, 243, 383, 490, 661, 772
MaeI CTAG 2 cut(s) 725, 1155
MaeII ACGT 3 cut(s) 323, 413, 912
MaeIII GTNAC 4 cut(s) 13, 602, 880, 913
MalI GATC 4 cut(s) 26, 292, 820, 1102
MboI GATC 4 cut(s) 24, 290, 818, 1100
MboII GAAGA 6 cut(s) 28, 352, 576, 588, 1038, 1425
MflI RGATCY 1 cut(s) 1100
MhlI GDGCHC 4 cut(s) 1012, 1180, 1252, 1324
MluCI AATT 4 cut(s) 8, 598, 926, 963
Mly113I GGCGCC 1 cut(s) 110
MmeI TCCRAC 4 cut(s) 83, 219, 691, 759
Mph1103I ATGCAT 1 cut(s) 612
MroXI GAANNNNTTC 1 cut(s) 580
MseI TTAA 4 cut(s) 225, 252, 833, 1559
MslI CAYNNNNRTG 1 cut(s) 498
MspCI CTTAAG 1 cut(s) 832
MspI CCGG 4 cut(s) 19, 336, 1295, 1338
MspR9I CCNGG 3 cut(s) 19, 107, 231
Mva1269I GAATGC 2 cut(s) 467, 610
MvaI CCWGG 2 cut(s) 107, 231
MwoI GCNNNNNNNGC 6 cut(s) 182, 509, 641, 884, 1190, 1523
NarI GGCGCC 1 cut(s) 110
NciI CCSGG 1 cut(s) 19
NcoI CCATGG 2 cut(s) 42, 734
NdeII GATC 4 cut(s) 24, 290, 818, 1100
NlaIII CATG 9 cut(s) 46, 74, 610, 738, 943, 1136, 1211, 1382, 1442
NlaIV GGNNCC 6 cut(s) 111, 741, 742, 815, 877, 1462
NmuCI GTSAC 3 cut(s) 13, 602, 880
NsiI ATGCAT 1 cut(s) 612
NspI RCATGY 3 cut(s) 74, 610, 1382
PaeI GCATGC 1 cut(s) 74
PagI TCATGA 1 cut(s) 939
PciSI GCTCTTC 1 cut(s) 571
PctI GAATGC 2 cut(s) 467, 610
PdmI GAANNNNTTC 1 cut(s) 580
PfeI GAWTC 4 cut(s) 332, 656, 787, 980
PkrI GCNGC 6 cut(s) 88, 149, 482, 1336, 1384, 1516
PluTI GGCGCC 1 cut(s) 113
Ppu21I YACGTR 1 cut(s) 414
PpuMI RGGWCCY 3 cut(s) 740, 1064, 1283
PshBI ATTAAT 1 cut(s) 252
PsiI TTATAA 2 cut(s) 128, 278
Psp124BI GAGCTC 3 cut(s) 1012, 1180, 1252
Psp1406I AACGTT 1 cut(s) 912
Psp5II RGGWCCY 3 cut(s) 740, 1064, 1283
Psp6I CCWGG 2 cut(s) 105, 229
PspGI CCWGG 2 cut(s) 105, 229
PspN4I GGNNCC 6 cut(s) 111, 741, 742, 815, 877, 1462
PspPI GGNCC 6 cut(s) 740, 814, 1064, 1136, 1211, 1283
PspPPI RGGWCCY 3 cut(s) 740, 1064, 1283
PsuI RGATCY 1 cut(s) 1100
RsaI GTAC 5 cut(s) 258, 723, 830, 857, 865
RsaNI GTAC 5 cut(s) 257, 722, 829, 856, 864
RseI CAYNNNNRTG 1 cut(s) 498
SacI GAGCTC 3 cut(s) 1012, 1180, 1252
SalI GTCGAC 1 cut(s) 134
SapI GCTCTTC 1 cut(s) 571
SaqAI TTAA 4 cut(s) 225, 252, 833, 1559
SatI GCNGC 6 cut(s) 87, 148, 481, 1335, 1383, 1515
Sau3AI GATC 4 cut(s) 24, 290, 818, 1100
Sau96I GGNCC 6 cut(s) 740, 814, 1064, 1136, 1211, 1283
ScaI AGTACT 2 cut(s) 723, 830
ScrFI CCNGG 3 cut(s) 19, 107, 231
SduI GDGCHC 4 cut(s) 1012, 1180, 1252, 1324
SfaNI GCATC 6 cut(s) 172, 243, 383, 490, 661, 772
SfcI CTRYAG 1 cut(s) 1452
SfoI GGCGCC 1 cut(s) 111
SinI GGWCC 5 cut(s) 740, 1064, 1136, 1211, 1283
SmiMI CAYNNNNRTG 1 cut(s) 498
SmlI CTYRAG 2 cut(s) 90, 832
SmoI CTYRAG 2 cut(s) 90, 832
SnaBI TACGTA 1 cut(s) 414
SphI GCATGC 1 cut(s) 74
Sse9I AATT 4 cut(s) 8, 598, 926, 963
SsiI CCGC 3 cut(s) 354, 1149, 1182
SspDI GGCGCC 1 cut(s) 109
SspMI CTAG 2 cut(s) 725, 1155
SstI GAGCTC 3 cut(s) 1012, 1180, 1252
StyD4I CCNGG 3 cut(s) 17, 105, 229
StyI CCWWGG 2 cut(s) 42, 734
TaaI ACNGT 7 cut(s) 121, 133, 273, 316, 754, 809, 1453
TaiI ACGT 3 cut(s) 326, 416, 915
TaqI TCGA 5 cut(s) 135, 180, 293, 749, 1470
TasI AATT 4 cut(s) 8, 598, 926, 963
TatI WGTACW 4 cut(s) 721, 828, 855, 863
TfiI GAWTC 4 cut(s) 332, 656, 787, 980
Tru1I TTAA 4 cut(s) 225, 252, 833, 1559
Tru9I TTAA 4 cut(s) 225, 252, 833, 1559
TscAI CASTG 3 cut(s) 447, 565, 709
TseFI GTSAC 3 cut(s) 13, 602, 880
TseI GCWGC 6 cut(s) 86, 147, 480, 1334, 1382, 1514
Tsp45I GTSAC 3 cut(s) 13, 602, 880
TspDTI ATGAA 3 cut(s) 363, 928, 956
TspGWI ACGGA 1 cut(s) 645
TspRI CASTG 3 cut(s) 447, 565, 709
Vha464I CTTAAG 1 cut(s) 832
VpaK11BI GGWCC 5 cut(s) 740, 1064, 1136, 1211, 1283
VspI ATTAAT 1 cut(s) 252
XagI CCTNNNNNAGG 1 cut(s) 1452
XapI RAATTY 2 cut(s) 8, 926
XceI RCATGY 3 cut(s) 74, 610, 1382
XmiI GTMKAC 1 cut(s) 135
XmnI GAANNNNTTC 1 cut(s) 580
XspI CTAG 2 cut(s) 725, 1155
ZrmI AGTACT 2 cut(s) 723, 830
Zsp2I ATGCAT 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.