pycom17g01010

rRNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002783
Physical Location & Seq
Forward (+)
719775 .. 721445
1671 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
ATGGATGAAGAATTCAATACCAAATTAGTGGGCCCAATCCTATCTTCCCTCCGGAAAAAGGAGATAGGGATAAATAGCAAAAATCAGAAAGGTCGAGAAGTCTCTTCACACCAATCACCAAAGTCACCATCCATGGCTGCCTCATCCGCACTCTGCAACTTGTTCTCCCTCCTCAGCCCCTCAAAGGCACAGCAACCCAAACTCCCACCGACCCAGCTCTCCATTTTACAGGCCCAGAAGCCCAGAGACCGGCTGGTTCCACTGGCTGCCCAAGGGGTCCACTCTGCTTCTTCTTCTTCGTCGATAGAGTCTGTGATTTGCCCTTCTCTGGCTTACGCCAACGTCCTATTCTTCAAGTCGGCGTACAATGTGCAGGTGATTGTCGACGACAACGAGCCCGAGGAGCGGCTGCTCGGCAGGTTTCGGCGGGAGGTCATGAAGGCCGGCGTTATTCAGGAGGTTAAGAGGAGGAGGTACTTTGAGAACAAGCAGGATGAGAAGAAGCGCCGGACCCGTGACGCCGCGAAGCGAAACAAGAGAAGCCGGCGACCCTTCTCGAGGCCCTTTCAGCAGAAGCCAGAAGTCCCTGAAGCCAAGAAGAGCGATGATGACGGAGATAACTGGGATCTTCCTCAAGGAGACATACCCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S21

Protein Analysis

218

Amino Acids

24.53

Weight (kDa)

9.86

Isoelectric Point (pI)

76.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S21 PF01165 125 - 178 5.4e-14 Ribosomal protein S21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 364
Acc36I ACCTGC 2 cut(s) 364, 408
AccBSI CCGCTC 1 cut(s) 406
AccI GTMKAC 1 cut(s) 384
AccII CGCG 1 cut(s) 524
AccIII TCCGGA 1 cut(s) 51
AciI CCGC 4 cut(s) 147, 406, 427, 522
AclWI GGATC 1 cut(s) 633
AcsI RAATTY 1 cut(s) 11
AcuI CTGAAG 1 cut(s) 609
AcyI GRCGYC 1 cut(s) 519
AfaI GTAC 2 cut(s) 365, 476
AfiI CCNNNNNNNGG 6 cut(s) 58, 184, 249, 328, 405, 558
AgsI TTSAA 2 cut(s) 16, 355
AjuI GAANNNNNNNTTGG 4 cut(s) 28, 60, 332, 364
AluBI AGCT 1 cut(s) 217
AluI AGCT 1 cut(s) 217
Alw26I GTCTC 3 cut(s) 106, 240, 633
AlwI GGATC 1 cut(s) 633
Ama87I CYCGRG 2 cut(s) 398, 556
Aor13HI TCCGGA 1 cut(s) 51
AoxI GGCC 4 cut(s) 31, 231, 441, 560
ApaI GGGCCC 1 cut(s) 35
ApeKI GCWGC 3 cut(s) 137, 266, 409
ApoI RAATTY 1 cut(s) 11
AspLEI GCGC 1 cut(s) 507
AspS9I GGNCC 6 cut(s) 31, 32, 232, 277, 510, 561
AsuHPI GGTGA 3 cut(s) 108, 117, 388
AvaI CYCGRG 2 cut(s) 398, 556
AvaII GGWCC 2 cut(s) 277, 510
BaeGI GKGCMC 1 cut(s) 35
BanII GRGCYC 2 cut(s) 35, 399
BbvCI CCTCAGC 1 cut(s) 173
BbvI GCAGC 3 cut(s) 124, 253, 396
BccI CCATC 1 cut(s) 136
BcoDI GTCTC 3 cut(s) 106, 240, 633
BfoI RGCGCY 1 cut(s) 508
BfuAI ACCTGC 2 cut(s) 364, 408
BisI GCNGC 5 cut(s) 138, 267, 407, 410, 522
BlsI GCNGC 5 cut(s) 139, 268, 408, 411, 523
Bme18I GGWCC 2 cut(s) 277, 510
BmeT110I CYCGRG 2 cut(s) 398, 556
BmgT120I GGNCC 6 cut(s) 31, 32, 232, 277, 510, 561
BmiI GGNNCC 4 cut(s) 33, 258, 278, 512
BmrI ACTGGG 1 cut(s) 631
BmuI ACTGGG 1 cut(s) 631
Bpu10I CCTNAGC 1 cut(s) 173
BpuEI CTTGAG 1 cut(s) 618
BsaHI GRCGYC 1 cut(s) 519
BsaI GGTCTC 1 cut(s) 240
BsaJI CCNNGG 3 cut(s) 132, 271, 399
BsaWI WCCGGW 1 cut(s) 51
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 186, 216
Bsc4I CCNNNNNNNGG 6 cut(s) 58, 184, 249, 328, 405, 558
Bse118I RCCGGY 3 cut(s) 249, 443, 543
Bse1I ACTGG 2 cut(s) 267, 626
BseAI TCCGGA 1 cut(s) 51
BseDI CCNNGG 3 cut(s) 132, 271, 399
BseGI GGATG 4 cut(s) 10, 128, 143, 499
BseLI CCNNNNNNNGG 6 cut(s) 58, 184, 249, 328, 405, 558
BseMII CTCAG 1 cut(s) 187
BseNI ACTGG 2 cut(s) 267, 626
BseRI GAGGAG 4 cut(s) 161, 416, 481, 484
BseSI GKGCMC 1 cut(s) 35
BseXI GCAGC 3 cut(s) 124, 253, 396
BseYI CCCAGC 1 cut(s) 213
BsgI GTGCAG 1 cut(s) 392
Bsh1236I CGCG 1 cut(s) 524
BshFI GGCC 4 cut(s) 33, 233, 443, 562
BsiHKCI CYCGRG 2 cut(s) 398, 556
BsiSI CCGG 5 cut(s) 52, 250, 444, 508, 544
BslFI GGGAC 1 cut(s) 569
BslI CCNNNNNNNGG 6 cut(s) 58, 184, 249, 328, 405, 558
BsmAI GTCTC 3 cut(s) 106, 240, 633
BsmFI GGGAC 1 cut(s) 569
BsnI GGCC 4 cut(s) 33, 233, 443, 562
Bso31I GGTCTC 1 cut(s) 240
BsoBI CYCGRG 2 cut(s) 398, 556
Bsp120I GGGCCC 1 cut(s) 31
Bsp1286I GDGCHC 2 cut(s) 35, 399
Bsp13I TCCGGA 1 cut(s) 51
Bsp143I GATC 1 cut(s) 625
Bsp19I CCATGG 1 cut(s) 132
BspACI CCGC 4 cut(s) 147, 406, 427, 522
BspANI GGCC 4 cut(s) 33, 233, 443, 562
BspCNI CTCAG 1 cut(s) 186
BspEI TCCGGA 1 cut(s) 51
BspFNI CGCG 1 cut(s) 524
BspHI TCATGA 1 cut(s) 435
BspLI GGNNCC 4 cut(s) 33, 258, 278, 512
BspMI ACCTGC 2 cut(s) 364, 408
BspPI GGATC 1 cut(s) 633
BspQI GCTCTTC 1 cut(s) 593
BspTNI GGTCTC 1 cut(s) 240
BsrBI CCGCTC 1 cut(s) 406
BsrFI RCCGGY 3 cut(s) 249, 443, 543
BsrI ACTGG 2 cut(s) 267, 626
BssAI RCCGGY 3 cut(s) 249, 443, 543
BssECI CCNNGG 3 cut(s) 132, 271, 399
BssMI GATC 1 cut(s) 625
BssNI GRCGYC 1 cut(s) 519
BssT1I CCWWGG 2 cut(s) 132, 271
Bst6I CTCTTC 2 cut(s) 109, 593
BstACI GRCGYC 1 cut(s) 519
BstC8I GCNNGC 2 cut(s) 445, 545
BstDEI CTNAG 1 cut(s) 173
BstDSI CCRYGG 1 cut(s) 132
BstENI CCTNNNNNAGG 1 cut(s) 556
BstF5I GGATG 4 cut(s) 10, 128, 143, 499
BstFNI CGCG 1 cut(s) 524
BstH2I RGCGCY 1 cut(s) 508
BstHHI GCGC 1 cut(s) 507
BstKTI GATC 1 cut(s) 628
BstMAI GTCTC 3 cut(s) 106, 240, 633
BstMBI GATC 1 cut(s) 625
BstMWI GCNNNNNNNGC 3 cut(s) 146, 403, 568
BstSLI GKGCMC 1 cut(s) 35
BstUI CGCG 1 cut(s) 524
BstV1I GCAGC 3 cut(s) 124, 253, 396
BstX2I RGATCY 1 cut(s) 625
BstXI CCANNNNNNTGG 1 cut(s) 28
BstYI RGATCY 1 cut(s) 625
BsuRI GGCC 4 cut(s) 33, 233, 443, 562
BtgI CCRYGG 1 cut(s) 132
BtgZI GCGATG 1 cut(s) 618
BtsCI GGATG 4 cut(s) 10, 128, 143, 499
BtsIMutI CAGTG 1 cut(s) 260
BveI ACCTGC 2 cut(s) 364, 408
Cac8I GCNNGC 2 cut(s) 445, 545
CciI TCATGA 1 cut(s) 435
CfoI GCGC 1 cut(s) 507
Cfr10I RCCGGY 3 cut(s) 249, 443, 543
Cfr13I GGNCC 6 cut(s) 31, 32, 232, 277, 510, 561
CseI GACGC 1 cut(s) 527
Csp6I GTAC 2 cut(s) 364, 475
CviAII CATG 2 cut(s) 133, 436
CviQI GTAC 2 cut(s) 364, 475
DdeI CTNAG 1 cut(s) 173
DpnI GATC 1 cut(s) 627
DpnII GATC 1 cut(s) 625
Eam1104I CTCTTC 2 cut(s) 109, 593
EarI CTCTTC 2 cut(s) 109, 593
Eco130I CCWWGG 2 cut(s) 132, 271
Eco24I GRGCYC 2 cut(s) 35, 399
Eco31I GGTCTC 1 cut(s) 240
Eco47I GGWCC 2 cut(s) 277, 510
Eco57I CTGAAG 1 cut(s) 609
Eco88I CYCGRG 2 cut(s) 398, 556
EcoNI CCTNNNNNAGG 1 cut(s) 556
EcoO109I RGGNCCY 1 cut(s) 561
EcoRI GAATTC 1 cut(s) 11
EcoT14I CCWWGG 2 cut(s) 132, 271
EcoT38I GRGCYC 2 cut(s) 35, 399
ErhI CCWWGG 2 cut(s) 132, 271
FaeI CATG 2 cut(s) 136, 439
FaiI YATR 3 cut(s) 134, 437, 644
FaqI GGGAC 1 cut(s) 569
FatI CATG 2 cut(s) 132, 435
FauI CCCGC 1 cut(s) 420
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 5 cut(s) 138, 267, 407, 410, 522
FokI GGATG 4 cut(s) 17, 115, 130, 506
FriOI GRGCYC 2 cut(s) 35, 399
Fsp4HI GCNGC 5 cut(s) 138, 267, 407, 410, 522
GlaI GCGC 1 cut(s) 506
GluI GCNGC 5 cut(s) 138, 267, 407, 410, 522
GsaI CCCAGC 1 cut(s) 217
HaeII RGCGCY 1 cut(s) 508
HaeIII GGCC 4 cut(s) 33, 233, 443, 562
HapII CCGG 5 cut(s) 52, 250, 444, 508, 544
HgaI GACGC 1 cut(s) 527
HhaI GCGC 1 cut(s) 507
Hin1I GRCGYC 1 cut(s) 519
Hin1II CATG 2 cut(s) 136, 439
Hin6I GCGC 1 cut(s) 505
HinP1I GCGC 1 cut(s) 505
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HinfI GANTC 1 cut(s) 308
HpaII CCGG 5 cut(s) 52, 250, 444, 508, 544
HphI GGTGA 3 cut(s) 108, 117, 388
Hpy166II GTNNAC 2 cut(s) 280, 385
Hpy188I TCNGA 1 cut(s) 87
Hpy188III TCNNGA 5 cut(s) 52, 95, 436, 455, 556
Hpy8I GTNNAC 2 cut(s) 280, 385
Hpy99I CGWCG 2 cut(s) 304, 389
HpyAV CCTTC 3 cut(s) 333, 433, 562
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 2 cut(s) 156, 373
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 403, 568
HpyF3I CTNAG 1 cut(s) 173
HpySE526I ACGT 1 cut(s) 342
Hsp92I GRCGYC 1 cut(s) 519
Hsp92II CATG 2 cut(s) 136, 439
HspAI GCGC 1 cut(s) 505
Kpn2I TCCGGA 1 cut(s) 51
KroI GCCGGC 2 cut(s) 443, 543
KroNI GCCGGC 2 cut(s) 445, 545
Kzo9I GATC 1 cut(s) 625
LguI GCTCTTC 1 cut(s) 593
LmnI GCTCC 1 cut(s) 403
Lsp1109I GCAGC 3 cut(s) 124, 253, 396
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 2 cut(s) 123, 515
MalI GATC 1 cut(s) 627
MbiI CCGCTC 1 cut(s) 406
MboI GATC 1 cut(s) 625
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 2 cut(s) 35, 399
MluCI AATT 2 cut(s) 11, 23
MlyI GAGTC 1 cut(s) 317
MroI TCCGGA 1 cut(s) 51
MroNI GCCGGC 2 cut(s) 443, 543
MseI TTAA 1 cut(s) 462
MspI CCGG 5 cut(s) 52, 250, 444, 508, 544
MvnI CGCG 1 cut(s) 524
MwoI GCNNNNNNNGC 3 cut(s) 146, 403, 568
NaeI GCCGGC 2 cut(s) 445, 545
NcoI CCATGG 1 cut(s) 132
NdeII GATC 1 cut(s) 625
NgoMIV GCCGGC 2 cut(s) 443, 543
NlaIII CATG 2 cut(s) 136, 439
NlaIV GGNNCC 4 cut(s) 33, 258, 278, 512
NmeAIII GCCGAG 1 cut(s) 393
NmuCI GTSAC 2 cut(s) 123, 515
PaeR7I CTCGAG 1 cut(s) 556
PagI TCATGA 1 cut(s) 435
PaqCI CACCTGC 1 cut(s) 364
PciSI GCTCTTC 1 cut(s) 593
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PdiI GCCGGC 2 cut(s) 445, 545
PkrI GCNGC 5 cut(s) 139, 268, 408, 411, 523
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PspFI CCCAGC 1 cut(s) 213
PspN4I GGNNCC 4 cut(s) 33, 258, 278, 512
PspOMI GGGCCC 1 cut(s) 31
PspPI GGNCC 6 cut(s) 31, 32, 232, 277, 510, 561
PsuI RGATCY 1 cut(s) 625
RsaI GTAC 2 cut(s) 365, 476
RsaNI GTAC 2 cut(s) 364, 475
SalI GTCGAC 1 cut(s) 383
SapI GCTCTTC 1 cut(s) 593
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 5 cut(s) 138, 267, 407, 410, 522
Sau3AI GATC 1 cut(s) 625
Sau96I GGNCC 6 cut(s) 31, 32, 232, 277, 510, 561
SchI GAGTC 1 cut(s) 317
SduI GDGCHC 2 cut(s) 35, 399
SetI ASST 8 cut(s) 94, 219, 345, 378, 422, 435, 462, 476
Sfr274I CTCGAG 1 cut(s) 556
SinI GGWCC 2 cut(s) 277, 510
SlaI CTCGAG 1 cut(s) 556
SmlI CTYRAG 2 cut(s) 556, 633
SmoI CTYRAG 2 cut(s) 556, 633
Sse9I AATT 2 cut(s) 11, 23
SsiI CCGC 4 cut(s) 147, 406, 427, 522
StyI CCWWGG 2 cut(s) 132, 271
TaiI ACGT 1 cut(s) 345
TaqI TCGA 4 cut(s) 94, 302, 384, 557
TasI AATT 2 cut(s) 11, 23
TauI GCSGC 2 cut(s) 409, 524
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TscAI CASTG 1 cut(s) 267
TseFI GTSAC 2 cut(s) 123, 515
TseI GCWGC 3 cut(s) 137, 266, 409
Tsp45I GTSAC 2 cut(s) 123, 515
TspDTI ATGAA 2 cut(s) 21, 452
TspGWI ACGGA 1 cut(s) 627
TspRI CASTG 1 cut(s) 267
VpaK11BI GGWCC 2 cut(s) 277, 510
XagI CCTNNNNNAGG 1 cut(s) 556
XapI RAATTY 1 cut(s) 11
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
XhoI CTCGAG 1 cut(s) 556
XmiI GTMKAC 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.