pycom17g21440

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001445
Physical Location & Seq
Reverse (-)
19795967 .. 19801174
5208 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1035 bp
ATGGATGTTATTTGTCAAGCAAAATCTGGGATGGGAAAAACTGCCGTCTTTGTTCTGTCAACCCTTCAGCAAATAGATCCAGTAGCTGGTCAGGTTGCTGCTCTTATTCTTTGCCACACAAGAGAATTGGCTTACCAGATTTGTCATGAATTCGAGAGGTTTAGTACTTATTTGCCGGACCTGAAGGTTGCAGTTTTCTATGGAGGTGTTAGTATCAAGGTTCACAAAGATTTGCTAAAAAATGAATGTCCTCACATTGTTGTTGGTACACCTGGGAGAGTATTAGCTTTGGCAAGAGACAAAGAGCTTGGACTGAAGAATGTCAGACATTTTATTCTTGATGAATGTGATAAGATGCTTGAGTCACTTGACATGAGGAGAGATGTGCAGGAGATATTTAAGTTGACTCCTCATGACAAGCAAGTGATGATGTTTTCAGCAACACTAAGCAAGGAAATACGCCCTGTTTGCAAGAAGTTTATGCAAGATCCCATGGAAATTTATGTGGATGATGAGGCCAAATTGACTCTTCATGGTCTCGTACAGCACTACATCAAATTGAGTGAGCCTGAGAAAAACCGCAAGTTGACTGACCTCCTTGATGCATTGGATTTCAACCAAGTTGTTATTTTTGTAAAGAGTGTAAGCAGGGCAGCTGAGCTGAACAAGTTACTTGCTGACTGTAACTTCCCCTCCATCTGCATTCACTCTGGAATGCCTCAGGAAGAAAGGCTAAAACGTTACAAGAATTTCAAGGAGGGGCTTTCAAGAATTCTTGTGGCAACAGATTTAGTAGGAAGGGGAATTGACATTGAGCGTGTTAACATTGTTATCAATTATGACATGCCTGATTCTGCTGATACCTACTTGCATAGGGTTGGCAGAGCTGGTAGGTTTGGTACCAAGGGGCTAGCAATTACATTTGTATCTTCTGCTTCTGATTCAGAAGTTCTTAATCAGGTCCAGTCAAGGTTTGAGGTGGATATAAAGGAGCTTCCCGAGCAAATTGATACTTCTACTTACATGCAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006974 GO:0007275 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009555 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033554 GO:0034613 GO:0034641 GO:0042623 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0090567 GO:0099402 GO:0140098 GO:1901360 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

345

Amino Acids

38.95

Weight (kDa)

6.38

Isoelectric Point (pI)

38.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 2 - 153 1.8e-36 DEAD/DEAH box helicase
ResIII PF04851 5 - 150 8.6e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 191 - 300 6.5e-30 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 899
AccB1I GGYRCC 1 cut(s) 899
AccB7I CCANNNNNTGG 1 cut(s) 86
AciI CCGC 1 cut(s) 580
AclI AACGTT 1 cut(s) 739
AclWI GGATC 2 cut(s) 71, 482
AcsI RAATTY 4 cut(s) 149, 498, 748, 771
AcuI CTGAAG 3 cut(s) 50, 203, 335
AfaI GTAC 4 cut(s) 166, 268, 543, 901
AfiI CCNNNNNNNGG 1 cut(s) 86
AgsI TTSAA 3 cut(s) 616, 754, 768
AjnI CCWGG 1 cut(s) 271
AluBI AGCT 7 cut(s) 86, 287, 307, 656, 661, 887, 994
AluI AGCT 7 cut(s) 86, 287, 307, 656, 661, 887, 994
Alw26I GTCTC 2 cut(s) 291, 542
AlwI GGATC 2 cut(s) 71, 482
AlwNI CAGNNNCTG 1 cut(s) 86
Ama87I CYCGRG 1 cut(s) 998
AoxI GGCC 1 cut(s) 516
ApeKI GCWGC 2 cut(s) 98, 653
ApoI RAATTY 4 cut(s) 149, 498, 748, 771
Asp718I GGTACC 1 cut(s) 899
AspS9I GGNCC 2 cut(s) 178, 961
AsuNHI GCTAGC 1 cut(s) 910
AvaI CYCGRG 1 cut(s) 998
AvaII GGWCC 2 cut(s) 178, 961
AxyI CCTNAGG 1 cut(s) 720
BaeI ACNNNNGTAYC 2 cut(s) 909, 942
BanI GGYRCC 1 cut(s) 899
BbvI GCAGC 2 cut(s) 85, 665
BccI CCATC 2 cut(s) 25, 704
BceAI ACGGC 1 cut(s) 29
BciT130I CCWGG 1 cut(s) 273
BcoDI GTCTC 2 cut(s) 291, 542
BfaI CTAG 1 cut(s) 911
BisI GCNGC 2 cut(s) 99, 654
BlpI GCTNAGC 1 cut(s) 657
BlsI GCNGC 2 cut(s) 100, 655
BmcAI AGTACT 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 273
Bme18I GGWCC 2 cut(s) 178, 961
BmeT110I CYCGRG 1 cut(s) 998
BmgT120I GGNCC 2 cut(s) 178, 961
BmiI GGNNCC 1 cut(s) 901
BmrFI CCNGG 1 cut(s) 273
BmsI GCATC 2 cut(s) 345, 592
BmtI GCTAGC 1 cut(s) 914
Bpu1102I GCTNAGC 1 cut(s) 657
BpuEI CTTGAG 1 cut(s) 380
BsaI GGTCTC 1 cut(s) 542
BsaJI CCNNGG 3 cut(s) 272, 492, 903
BsaXI ACNNNNNCTCC 2 cut(s) 677, 707
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 2 cut(s) 80, 964
Bse21I CCTNAGG 1 cut(s) 720
BseBI CCWGG 1 cut(s) 273
BseDI CCNNGG 3 cut(s) 272, 492, 903
BseGI GGATG 3 cut(s) 10, 36, 514
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMII CTCAG 3 cut(s) 561, 648, 734
BseNI ACTGG 2 cut(s) 80, 964
BseRI GAGGAG 2 cut(s) 391, 399
BseXI GCAGC 2 cut(s) 85, 665
BsgI GTGCAG 1 cut(s) 407
BshFI GGCC 1 cut(s) 518
BshNI GGYRCC 1 cut(s) 899
BsiHKCI CYCGRG 1 cut(s) 998
BsiSI CCGG 1 cut(s) 176
BslI CCNNNNNNNGG 1 cut(s) 86
BsmAI GTCTC 2 cut(s) 291, 542
BsmI GAATGC 2 cut(s) 702, 720
BsnI GGCC 1 cut(s) 518
Bso31I GGTCTC 1 cut(s) 542
BsoBI CYCGRG 1 cut(s) 998
Bsp143I GATC 2 cut(s) 76, 487
Bsp1720I GCTNAGC 1 cut(s) 657
Bsp19I CCATGG 1 cut(s) 492
BspACI CCGC 1 cut(s) 580
BspANI GGCC 1 cut(s) 518
BspCNI CTCAG 3 cut(s) 562, 649, 733
BspHI TCATGA 2 cut(s) 145, 412
BspLI GGNNCC 1 cut(s) 901
BspOI GCTAGC 1 cut(s) 914
BspPI GGATC 2 cut(s) 71, 482
BspT107I GGYRCC 1 cut(s) 899
BspTNI GGTCTC 1 cut(s) 542
BsrI ACTGG 2 cut(s) 80, 964
BssECI CCNNGG 3 cut(s) 272, 492, 903
BssMI GATC 2 cut(s) 76, 487
BssT1I CCWWGG 2 cut(s) 492, 903
Bst2UI CCWGG 1 cut(s) 273
Bst4CI ACNGT 1 cut(s) 683
Bst6I CTCTTC 1 cut(s) 534
BstC8I GCNNGC 1 cut(s) 912
BstDEI CTNAG 4 cut(s) 446, 570, 657, 720
BstDSI CCRYGG 1 cut(s) 492
BstF5I GGATG 3 cut(s) 10, 36, 514
BstKTI GATC 2 cut(s) 79, 490
BstMAI GTCTC 2 cut(s) 291, 542
BstMBI GATC 2 cut(s) 76, 487
BstMWI GCNNNNNNNGC 2 cut(s) 468, 1000
BstNI CCWGG 1 cut(s) 273
BstNSI RCATGY 2 cut(s) 847, 1027
BstSCI CCNGG 1 cut(s) 271
BstV1I GCAGC 2 cut(s) 85, 665
BstX2I RGATCY 2 cut(s) 76, 487
BstYI RGATCY 2 cut(s) 76, 487
Bsu36I CCTNAGG 1 cut(s) 720
BsuRI GGCC 1 cut(s) 518
BtgI CCRYGG 1 cut(s) 492
BtsCI GGATG 3 cut(s) 10, 36, 514
Cac8I GCNNGC 1 cut(s) 912
CaiI CAGNNNCTG 1 cut(s) 86
CciI TCATGA 2 cut(s) 145, 412
Cfr13I GGNCC 2 cut(s) 178, 961
Csp6I GTAC 4 cut(s) 165, 267, 542, 900
CviAII CATG 7 cut(s) 146, 373, 413, 493, 533, 844, 1024
CviQI GTAC 4 cut(s) 165, 267, 542, 900
DdeI CTNAG 4 cut(s) 446, 570, 657, 720
DpnI GATC 2 cut(s) 78, 489
DpnII GATC 2 cut(s) 76, 487
Eam1104I CTCTTC 1 cut(s) 534
EarI CTCTTC 1 cut(s) 534
Eco130I CCWWGG 2 cut(s) 492, 903
Eco31I GGTCTC 1 cut(s) 542
Eco47I GGWCC 2 cut(s) 178, 961
Eco57I CTGAAG 3 cut(s) 50, 203, 335
Eco81I CCTNAGG 1 cut(s) 720
Eco88I CYCGRG 1 cut(s) 998
EcoRI GAATTC 2 cut(s) 149, 771
EcoRII CCWGG 1 cut(s) 271
EcoT14I CCWWGG 2 cut(s) 492, 903
EcoT22I ATGCAT 1 cut(s) 607
ErhI CCWWGG 2 cut(s) 492, 903
FaeI CATG 7 cut(s) 149, 376, 416, 496, 536, 847, 1027
FatI CATG 7 cut(s) 145, 372, 412, 492, 532, 843, 1023
Fnu4HI GCNGC 2 cut(s) 99, 654
FokI GGATG 3 cut(s) 17, 43, 521
Fsp4HI GCNGC 2 cut(s) 99, 654
FspBI CTAG 1 cut(s) 911
GluI GCNGC 2 cut(s) 99, 654
HaeIII GGCC 1 cut(s) 518
HapII CCGG 1 cut(s) 176
Hin1II CATG 7 cut(s) 149, 376, 416, 496, 536, 847, 1027
HincII GTYRAC 4 cut(s) 60, 405, 588, 823
HindII GTYRAC 4 cut(s) 60, 405, 588, 823
HinfI GANTC 5 cut(s) 362, 406, 526, 851, 941
HpaI GTTAAC 1 cut(s) 823
HpaII CCGG 1 cut(s) 176
Hpy166II GTNNAC 6 cut(s) 60, 223, 269, 405, 588, 823
Hpy188I TCNGA 3 cut(s) 326, 940, 946
Hpy188III TCNNGA 8 cut(s) 146, 154, 338, 413, 711, 722, 768, 998
Hpy8I GTNNAC 6 cut(s) 60, 223, 269, 405, 588, 823
HpyAV CCTTC 3 cut(s) 74, 178, 792
HpyCH4III ACNGT 1 cut(s) 683
HpyCH4IV ACGT 1 cut(s) 739
HpyCH4V TGCA 8 cut(s) 191, 388, 471, 484, 605, 702, 871, 1027
HpyF10VI GCNNNNNNNGC 2 cut(s) 468, 1000
HpyF3I CTNAG 4 cut(s) 446, 570, 657, 720
HpySE526I ACGT 1 cut(s) 739
Hsp92II CATG 7 cut(s) 149, 376, 416, 496, 536, 847, 1027
KpnI GGTACC 1 cut(s) 903
KspAI GTTAAC 1 cut(s) 823
Kzo9I GATC 2 cut(s) 76, 487
LmnI GCTCC 1 cut(s) 991
Lsp1109I GCAGC 2 cut(s) 85, 665
LweI GCATC 2 cut(s) 345, 592
MaeI CTAG 1 cut(s) 911
MaeII ACGT 1 cut(s) 739
MaeIII GTNAC 4 cut(s) 363, 669, 683, 740
MalI GATC 2 cut(s) 78, 489
MboI GATC 2 cut(s) 76, 487
MboII GAAGA 4 cut(s) 328, 521, 737, 921
MflI RGATCY 2 cut(s) 76, 487
MlyI GAGTC 3 cut(s) 371, 400, 520
Mph1103I ATGCAT 1 cut(s) 607
MseI TTAA 3 cut(s) 399, 822, 954
MslI CAYNNNNRTG 1 cut(s) 1028
MspA1I CMGCKG 1 cut(s) 656
MspI CCGG 1 cut(s) 176
MspR9I CCNGG 1 cut(s) 273
Mva1269I GAATGC 2 cut(s) 702, 720
MvaI CCWGG 1 cut(s) 273
MwoI GCNNNNNNNGC 2 cut(s) 468, 1000
NcoI CCATGG 1 cut(s) 492
NdeII GATC 2 cut(s) 76, 487
NheI GCTAGC 1 cut(s) 910
NlaIII CATG 7 cut(s) 149, 376, 416, 496, 536, 847, 1027
NlaIV GGNNCC 1 cut(s) 901
NmuCI GTSAC 1 cut(s) 363
NsiI ATGCAT 1 cut(s) 607
NspI RCATGY 2 cut(s) 847, 1027
PagI TCATGA 2 cut(s) 145, 412
PctI GAATGC 2 cut(s) 702, 720
PfeI GAWTC 2 cut(s) 851, 941
PflMI CCANNNNNTGG 1 cut(s) 86
PkrI GCNGC 2 cut(s) 100, 655
PleI GAGTC 3 cut(s) 370, 400, 520
PpsI GAGTC 3 cut(s) 370, 400, 520
Psp1406I AACGTT 1 cut(s) 739
Psp6I CCWGG 1 cut(s) 271
PspGI CCWGG 1 cut(s) 271
PspN4I GGNNCC 1 cut(s) 901
PspPI GGNCC 2 cut(s) 178, 961
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 2 cut(s) 76, 487
PvuII CAGCTG 1 cut(s) 656
RsaI GTAC 4 cut(s) 166, 268, 543, 901
RsaNI GTAC 4 cut(s) 165, 267, 542, 900
RseI CAYNNNNRTG 1 cut(s) 1028
SaqAI TTAA 3 cut(s) 399, 822, 954
SatI GCNGC 2 cut(s) 99, 654
Sau3AI GATC 2 cut(s) 76, 487
Sau96I GGNCC 2 cut(s) 178, 961
ScaI AGTACT 1 cut(s) 166
SchI GAGTC 3 cut(s) 371, 400, 520
ScrFI CCNGG 1 cut(s) 273
SfaNI GCATC 2 cut(s) 345, 592
SinI GGWCC 2 cut(s) 178, 961
SmiMI CAYNNNNRTG 1 cut(s) 1028
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
SsiI CCGC 1 cut(s) 580
SspMI CTAG 1 cut(s) 911
StyD4I CCNGG 1 cut(s) 271
StyI CCWWGG 2 cut(s) 492, 903
TaaI ACNGT 1 cut(s) 683
TaiI ACGT 1 cut(s) 742
TaqI TCGA 1 cut(s) 153
TatI WGTACW 1 cut(s) 164
TfiI GAWTC 2 cut(s) 851, 941
Tru1I TTAA 3 cut(s) 399, 822, 954
Tru9I TTAA 3 cut(s) 399, 822, 954
TseFI GTSAC 1 cut(s) 363
TseI GCWGC 2 cut(s) 98, 653
Tsp45I GTSAC 1 cut(s) 363
TspDTI ATGAA 4 cut(s) 162, 258, 357, 521
Van91I CCANNNNNTGG 1 cut(s) 86
VpaK11BI GGWCC 2 cut(s) 178, 961
XapI RAATTY 4 cut(s) 149, 498, 748, 771
XceI RCATGY 2 cut(s) 847, 1027
XspI CTAG 1 cut(s) 911
ZrmI AGTACT 1 cut(s) 166
Zsp2I ATGCAT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.