pycom17g22150

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001734
Physical Location & Seq
Reverse (-)
20532770 .. 20536700
3931 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1362 bp
ATGGCTTCCATATCGGTGGACATCGCCACGACCCTTATCGGGCTAACTTCTCGCCGCTCCACCCGACTCCCCAGGGTCGGTGCCGTAGCCCGCTTTCCCTTCACGTGGAGACCGAGGACTTTCGCACGCTCCCAAACCCTACACTACTCGGCGTCGTCGTTTTGCCGAGCTCTTGTCGCCAAGCCCAACCGAGGCTACAGAAGGTTCACTGCGGCTGCGAGCAGCGCAACGACGTCGCAGAGCGAGAGCTCCGACGTTTTGACCCAGATTGAGCCGGATAATCGCATCCCCGCCACCATAATCACCGGTTTCTTGGGCTCCGGAAAGACCACATTGCTTAACCATATATTGACTGCGGAACACGGGAAGCGAATCGCGGTGATTGAGAACGAGTATGGTGAGATAGATATTGATGGTTCTTTGGTTGCTGCGAAAACCACTGGGGCCGAAGACATTGTTATGTTGAATAATGGTTGCCTTTGCTGCACTGTGAGGGGTGATCTTGTGCGGATGATTGCGGACTTGGTGAATAAGAAGAAAGGGAAGTTTGATCATATTGTTATAGAGACTACTGGATTGGCAAATCCTGCACCGATCATTCAAACCTTTTATGCTGAAGATCAGGTTTTTAATGATGTCAAGTTGGATGGTGTTGTAACGTTAGTTGATGCTAAGCATGCTGTTTTTCATTTGGATGAAGTTAAACCAAAAGGAGTTGTCAATGAGGCTATAGAGCAGATTGCTTATGCTGACCGTATCATTGTCAATAAGACCGATCTTGTTGGTGAGCCAGAAATTGCTTCTTTGGTTCAACGAATTAAGAACATCAATGGAATGGCTCAATTGAAGCGGACTGAGTTTGGGAAGGTTAACCTGGATTATGTTCTTGGTATTGGAGGATTTGATTTGGAGAGGATAGAGAGTGCTATTGATGCTGAAGGTGAAAAGGAAGATCATGACCATCACCATGATCATGACCATGACCATCATCACCATCATGATGAGAAACACGATCACAAGCATGATCATCATGATGATCACCACGCCCATGATCATGTTCATGATCCTGGTGTTTCTTCTGTCAGCATAGTTTGTGAAGGGAACTTAGATCTTGAAAAGGCTAACATATGGCTTGGTACGCTGTTGTTGGAACGAAGCGAGGACATATATCGTATGAAAGGTCTTCTATCTGTTCAAGGAATGAAAGAGAGATTTGTCTTCCAGGGAGTTCACGATATATTCCAAGGCTCGCCTGATCGGCTGTGGGGCCCCAACGAGCCAAGAACGAACAAGATTGTTTTCATAGGGAAGAACTTGGATGCGCAGGAGTTGGAAAAGGGTTTTAAAGGCTGTTTACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

454

Amino Acids

50.35

Weight (kDa)

6.04

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 97 - 281 6.9e-59 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 358 - 452 2.7e-22 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 236
Acc16I TGCGCA 1 cut(s) 1323
AccB1I GGYRCC 1 cut(s) 80
AccBSI CCGCTC 1 cut(s) 57
AccII CGCG 1 cut(s) 377
AccIII TCCGGA 1 cut(s) 320
AciI CCGC 9 cut(s) 55, 91, 212, 291, 356, 377, 508, 518, 850
AclI AACGTT 1 cut(s) 659
AclWI GGATC 1 cut(s) 1058
AcuI CTGAAG 2 cut(s) 636, 957
AcvI CACGTG 1 cut(s) 105
AcyI GRCGYC 2 cut(s) 152, 233
AfaI GTAC 1 cut(s) 1138
AfiI CCNNNNNNNGG 4 cut(s) 39, 77, 105, 191
AgeI ACCGGT 1 cut(s) 305
AgsI TTSAA 6 cut(s) 466, 602, 812, 847, 1115, 1196
AjnI CCWGG 4 cut(s) 71, 873, 1066, 1221
AluBI AGCT 2 cut(s) 170, 249
AluI AGCT 2 cut(s) 170, 249
Alw21I GWGCWC 2 cut(s) 172, 251
Alw26I GTCTC 2 cut(s) 103, 560
AlwI GGATC 1 cut(s) 1058
Aor13HI TCCGGA 1 cut(s) 320
AoxI GGCC 2 cut(s) 444, 1267
ApaI GGGCCC 1 cut(s) 1271
ApeKI GCWGC 4 cut(s) 215, 222, 428, 483
AsiGI ACCGGT 1 cut(s) 305
AspLEI GCGC 2 cut(s) 227, 1324
AspS9I GGNCC 3 cut(s) 444, 1267, 1268
BaeGI GKGCMC 1 cut(s) 1271
BanI GGYRCC 1 cut(s) 80
BanII GRGCYC 4 cut(s) 172, 251, 320, 1271
BbrPI CACGTG 1 cut(s) 105
BbsI GAAGAC 3 cut(s) 456, 1175, 1210
Bbv12I GWGCWC 2 cut(s) 172, 251
BbvI GCAGC 4 cut(s) 202, 234, 415, 470
BccI CCATC 5 cut(s) 407, 641, 969, 993, 1002
BceAI ACGGC 1 cut(s) 68
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BciT130I CCWGG 4 cut(s) 73, 875, 1068, 1223
BclI TGATCA 5 cut(s) 550, 970, 1024, 1036, 1051
BcoDI GTCTC 2 cut(s) 103, 560
BfmI CTRYAG 2 cut(s) 196, 729
BglI GCCNNNNNGGC 1 cut(s) 1258
BglII AGATCT 1 cut(s) 1108
BisI GCNGC 6 cut(s) 55, 213, 216, 223, 429, 484
BlpI GCTNAGC 1 cut(s) 672
BlsI GCNGC 6 cut(s) 56, 214, 217, 224, 430, 485
Bme1390I CCNGG 4 cut(s) 73, 875, 1068, 1223
BmgT120I GGNCC 3 cut(s) 444, 1267, 1268
BmiI GGNNCC 6 cut(s) 82, 319, 445, 1268, 1269, 1270
BmrFI CCNGG 4 cut(s) 73, 875, 1068, 1223
BmrI ACTGGG 1 cut(s) 450
BmsI GCATC 4 cut(s) 294, 658, 922, 1309
BmuI ACTGGG 1 cut(s) 450
BpiI GAAGAC 3 cut(s) 456, 1175, 1210
Bpu1102I GCTNAGC 1 cut(s) 672
BsaAI YACGTR 1 cut(s) 105
BsaHI GRCGYC 2 cut(s) 152, 233
BsaI GGTCTC 1 cut(s) 103
BsaJI CCNNGG 6 cut(s) 71, 72, 113, 190, 1222, 1243
BsaWI WCCGGW 2 cut(s) 305, 320
Bsc4I CCNNNNNNNGG 4 cut(s) 39, 77, 105, 191
Bse118I RCCGGY 1 cut(s) 305
Bse1I ACTGG 2 cut(s) 445, 577
Bse3DI GCAATG 1 cut(s) 332
BseAI TCCGGA 1 cut(s) 320
BseBI CCWGG 4 cut(s) 73, 875, 1068, 1223
BseDI CCNNGG 6 cut(s) 71, 72, 113, 190, 1222, 1243
BseGI GGATG 5 cut(s) 285, 516, 652, 700, 1324
BseLI CCNNNNNNNGG 4 cut(s) 39, 77, 105, 191
BseMI GCAATG 1 cut(s) 332
BseMII CTCAG 1 cut(s) 846
BseNI ACTGG 2 cut(s) 445, 577
BseSI GKGCMC 1 cut(s) 1271
BseXI GCAGC 4 cut(s) 202, 234, 415, 470
BsgI GTGCAG 2 cut(s) 469, 573
Bsh1236I CGCG 1 cut(s) 377
BshFI GGCC 2 cut(s) 446, 1269
BshNI GGYRCC 1 cut(s) 80
BshTI ACCGGT 1 cut(s) 305
BsiHKAI GWGCWC 2 cut(s) 172, 251
BsiSI CCGG 3 cut(s) 275, 306, 321
BslI CCNNNNNNNGG 4 cut(s) 39, 77, 105, 191
BsmAI GTCTC 2 cut(s) 103, 560
BsnI GGCC 2 cut(s) 446, 1269
Bso31I GGTCTC 1 cut(s) 103
Bsp120I GGGCCC 1 cut(s) 1267
Bsp1286I GDGCHC 4 cut(s) 172, 251, 320, 1271
Bsp13I TCCGGA 1 cut(s) 320
Bsp1720I GCTNAGC 1 cut(s) 672
BspACI CCGC 9 cut(s) 55, 91, 212, 291, 356, 377, 508, 518, 850
BspANI GGCC 2 cut(s) 446, 1269
BspCNI CTCAG 1 cut(s) 847
BspEI TCCGGA 1 cut(s) 320
BspFNI CGCG 1 cut(s) 377
BspHI TCATGA 5 cut(s) 955, 973, 997, 1030, 1060
BspLI GGNNCC 6 cut(s) 82, 319, 445, 1268, 1269, 1270
BspPI GGATC 1 cut(s) 1058
BspT107I GGYRCC 1 cut(s) 80
BspTNI GGTCTC 1 cut(s) 103
BsrBI CCGCTC 1 cut(s) 57
BsrDI GCAATG 1 cut(s) 332
BsrFI RCCGGY 1 cut(s) 305
BsrI ACTGG 2 cut(s) 445, 577
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 6 cut(s) 71, 72, 113, 190, 1222, 1243
BssNI GRCGYC 2 cut(s) 152, 233
BssT1I CCWWGG 1 cut(s) 1243
Bst2UI CCWGG 4 cut(s) 73, 875, 1068, 1223
Bst4CI ACNGT 3 cut(s) 490, 755, 1359
BstACI GRCGYC 2 cut(s) 152, 233
BstAPI GCANNNNNTGC 1 cut(s) 587
BstBAI YACGTR 1 cut(s) 105
BstC8I GCNNGC 5 cut(s) 91, 127, 220, 678, 1250
BstDEI CTNAG 3 cut(s) 672, 855, 1105
BstF5I GGATG 5 cut(s) 285, 516, 652, 700, 1324
BstFNI CGCG 1 cut(s) 377
BstHHI GCGC 2 cut(s) 227, 1324
BstMAI GTCTC 2 cut(s) 103, 560
BstMWI GCNNNNNNNGC 8 cut(s) 176, 224, 483, 587, 677, 932, 1138, 1258
BstNI CCWGG 4 cut(s) 73, 875, 1068, 1223
BstNSI RCATGY 1 cut(s) 680
BstSCI CCNGG 4 cut(s) 71, 873, 1066, 1221
BstSFI CTRYAG 2 cut(s) 196, 729
BstSLI GKGCMC 1 cut(s) 1271
BstUI CGCG 1 cut(s) 377
BstV1I GCAGC 4 cut(s) 202, 234, 415, 470
BstV2I GAAGAC 3 cut(s) 456, 1175, 1210
BstX2I RGATCY 1 cut(s) 1108
BstXI CCANNNNNNTGG 1 cut(s) 16
BstYI RGATCY 1 cut(s) 1108
BsuRI GGCC 2 cut(s) 446, 1269
BtgZI GCGATG 1 cut(s) 7
BtsCI GGATG 5 cut(s) 285, 516, 652, 700, 1324
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 3 cut(s) 207, 438, 486
Cac8I GCNNGC 5 cut(s) 91, 127, 220, 678, 1250
CciI TCATGA 5 cut(s) 955, 973, 997, 1030, 1060
CfoI GCGC 2 cut(s) 227, 1324
Cfr10I RCCGGY 1 cut(s) 305
Cfr13I GGNCC 3 cut(s) 444, 1267, 1268
CseI GACGC 1 cut(s) 141
Csp6I GTAC 1 cut(s) 1137
CspAI ACCGGT 1 cut(s) 305
CviQI GTAC 1 cut(s) 1137
DdeI CTNAG 3 cut(s) 672, 855, 1105
DraI TTTAAA 1 cut(s) 1345
Ecl136II GAGCTC 2 cut(s) 170, 249
Eco130I CCWWGG 1 cut(s) 1243
Eco24I GRGCYC 4 cut(s) 172, 251, 320, 1271
Eco31I GGTCTC 1 cut(s) 103
Eco53kI GAGCTC 2 cut(s) 170, 249
Eco57I CTGAAG 2 cut(s) 636, 957
Eco72I CACGTG 1 cut(s) 105
EcoICRI GAGCTC 2 cut(s) 170, 249
EcoO109I RGGNCCY 2 cut(s) 1267, 1268
EcoRII CCWGG 4 cut(s) 71, 873, 1066, 1221
EcoT14I CCWWGG 1 cut(s) 1243
EcoT38I GRGCYC 4 cut(s) 172, 251, 320, 1271
ErhI CCWWGG 1 cut(s) 1243
FauI CCCGC 2 cut(s) 98, 298
FauNDI CATATG 1 cut(s) 1127
FbaI TGATCA 5 cut(s) 550, 970, 1024, 1036, 1051
Fnu4HI GCNGC 6 cut(s) 55, 213, 216, 223, 429, 484
FokI GGATG 5 cut(s) 272, 523, 659, 707, 1331
FriOI GRGCYC 4 cut(s) 172, 251, 320, 1271
Fsp4HI GCNGC 6 cut(s) 55, 213, 216, 223, 429, 484
FspI TGCGCA 1 cut(s) 1323
GlaI GCGC 2 cut(s) 226, 1323
GluI GCNGC 6 cut(s) 55, 213, 216, 223, 429, 484
HaeIII GGCC 2 cut(s) 446, 1269
HapII CCGG 3 cut(s) 275, 306, 321
HgaI GACGC 1 cut(s) 141
HhaI GCGC 2 cut(s) 227, 1324
Hin1I GRCGYC 2 cut(s) 152, 233
Hin6I GCGC 2 cut(s) 225, 1322
HinP1I GCGC 2 cut(s) 225, 1322
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HinfI GANTC 2 cut(s) 66, 372
HpaI GTTAAC 1 cut(s) 871
HpaII CCGG 3 cut(s) 275, 306, 321
Hpy166II GTNNAC 5 cut(s) 19, 207, 871, 1231, 1355
Hpy188I TCNGA 1 cut(s) 253
Hpy188III TCNNGA 8 cut(s) 321, 956, 974, 998, 1031, 1061, 1112, 1232
Hpy8I GTNNAC 5 cut(s) 19, 207, 871, 1231, 1355
Hpy99I CGWCG 5 cut(s) 157, 160, 235, 238, 257
HpyAV CCTTC 5 cut(s) 109, 195, 859, 932, 1091
HpyCH4III ACNGT 3 cut(s) 490, 755, 1359
HpyCH4IV ACGT 4 cut(s) 104, 233, 255, 659
HpyCH4V TGCA 2 cut(s) 486, 590
HpyF10VI GCNNNNNNNGC 8 cut(s) 176, 224, 483, 587, 677, 932, 1138, 1258
HpyF3I CTNAG 3 cut(s) 672, 855, 1105
HpySE526I ACGT 4 cut(s) 104, 233, 255, 659
Hsp92I GRCGYC 2 cut(s) 152, 233
HspAI GCGC 2 cut(s) 225, 1322
Kpn2I TCCGGA 1 cut(s) 320
Ksp22I TGATCA 5 cut(s) 550, 970, 1024, 1036, 1051
KspAI GTTAAC 1 cut(s) 871
LmnI GCTCC 4 cut(s) 62, 134, 254, 323
Lsp1109I GCAGC 4 cut(s) 202, 234, 415, 470
LweI GCATC 4 cut(s) 294, 658, 922, 1309
MaeII ACGT 4 cut(s) 104, 233, 255, 659
MaeIII GTNAC 1 cut(s) 655
MbiI CCGCTC 1 cut(s) 57
MboII GAAGA 8 cut(s) 461, 547, 629, 962, 1068, 1175, 1210, 1321
MfeI CAATTG 1 cut(s) 842
MflI RGATCY 1 cut(s) 1108
MhlI GDGCHC 4 cut(s) 172, 251, 320, 1271
MluCI AATT 3 cut(s) 795, 816, 842
MlyI GAGTC 1 cut(s) 60
MmeI TCCRAC 4 cut(s) 276, 624, 1128, 1311
MnlI CCTC 7 cut(s) 108, 185, 486, 718, 890, 906, 1153
MroI TCCGGA 1 cut(s) 320
MseI TTAA 6 cut(s) 339, 630, 702, 819, 870, 1344
MspI CCGG 3 cut(s) 275, 306, 321
MspR9I CCNGG 4 cut(s) 73, 875, 1068, 1223
MunI CAATTG 1 cut(s) 842
MvaI CCWGG 4 cut(s) 73, 875, 1068, 1223
MvnI CGCG 1 cut(s) 377
MwoI GCNNNNNNNGC 8 cut(s) 176, 224, 483, 587, 677, 932, 1138, 1258
NdeI CATATG 1 cut(s) 1127
NlaIV GGNNCC 6 cut(s) 82, 319, 445, 1268, 1269, 1270
NmeAIII GCCGAG 2 cut(s) 128, 191
NsbI TGCGCA 1 cut(s) 1323
NspI RCATGY 1 cut(s) 680
PaeI GCATGC 1 cut(s) 680
PagI TCATGA 5 cut(s) 955, 973, 997, 1030, 1060
PasI CCCWGGG 1 cut(s) 72
PcsI WCGNNNNNNNCGW 2 cut(s) 110, 155
PfeI GAWTC 1 cut(s) 372
PinAI ACCGGT 1 cut(s) 305
PkrI GCNGC 6 cut(s) 56, 214, 217, 224, 430, 485
PleI GAGTC 1 cut(s) 60
PmaCI CACGTG 1 cut(s) 105
PmlI CACGTG 1 cut(s) 105
PpsI GAGTC 1 cut(s) 60
Ppu21I YACGTR 1 cut(s) 105
Psp124BI GAGCTC 2 cut(s) 172, 251
Psp1406I AACGTT 1 cut(s) 659
Psp6I CCWGG 4 cut(s) 71, 873, 1066, 1221
PspCI CACGTG 1 cut(s) 105
PspGI CCWGG 4 cut(s) 71, 873, 1066, 1221
PspN4I GGNNCC 6 cut(s) 82, 319, 445, 1268, 1269, 1270
PspOMI GGGCCC 1 cut(s) 1267
PspPI GGNCC 3 cut(s) 444, 1267, 1268
PsuI RGATCY 1 cut(s) 1108
RsaI GTAC 1 cut(s) 1138
RsaNI GTAC 1 cut(s) 1137
SacI GAGCTC 2 cut(s) 172, 251
SaqAI TTAA 6 cut(s) 339, 630, 702, 819, 870, 1344
SatI GCNGC 6 cut(s) 55, 213, 216, 223, 429, 484
Sau96I GGNCC 3 cut(s) 444, 1267, 1268
SchI GAGTC 1 cut(s) 60
ScrFI CCNGG 4 cut(s) 73, 875, 1068, 1223
SduI GDGCHC 4 cut(s) 172, 251, 320, 1271
SfaNI GCATC 4 cut(s) 294, 658, 922, 1309
SfcI CTRYAG 2 cut(s) 196, 729
SphI GCATGC 1 cut(s) 680
Sse9I AATT 3 cut(s) 795, 816, 842
SsiI CCGC 9 cut(s) 55, 91, 212, 291, 356, 377, 508, 518, 850
SstI GAGCTC 2 cut(s) 172, 251
StyD4I CCNGG 4 cut(s) 71, 873, 1066, 1221
StyI CCWWGG 1 cut(s) 1243
TaaI ACNGT 3 cut(s) 490, 755, 1359
TaiI ACGT 4 cut(s) 107, 236, 258, 662
TaqII GACCGA 2 cut(s) 127, 788
TasI AATT 3 cut(s) 795, 816, 842
TauI GCSGC 2 cut(s) 57, 215
TfiI GAWTC 1 cut(s) 372
Tru1I TTAA 6 cut(s) 339, 630, 702, 819, 870, 1344
Tru9I TTAA 6 cut(s) 339, 630, 702, 819, 870, 1344
TscAI CASTG 3 cut(s) 214, 445, 493
TseI GCWGC 4 cut(s) 215, 222, 428, 483
TspDTI ATGAA 6 cut(s) 677, 711, 1049, 1190, 1217, 1291
TspRI CASTG 3 cut(s) 214, 445, 493
XceI RCATGY 1 cut(s) 680
ZraI GACGTC 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.