Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0002169

Physical Location & Seq

Genomic Coordinates Forward (+)

21016075 .. 21020254

Gene Structure 4180 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1020 bp

ATGCCTGCCGTTAGCTCGTCAGTTTCTGGCCACGGCCAAACTATTTGTGTCACCGGAGCTGGAGGCTTCATTGCTTCTTGGATGGTGAAGCTATTGCTCGAAAGAGGCTACACTGTTAGAGGAACCTTGAGAAACCCAGATGACCCAAAGAATGCTCATTTGAGAGAGCTTGAAGGAGCTGCAGAGAGACTAACTTTGTGCAGAGCTGATCTCCTTGATTATGAGAGCCTCAAAGAAGCTATTAACGGCTGTGATGGAGTTTTTCACACAGCATCACCTGTCACGGATGATCCAGAACAAATGGTGGAGCCCGCAGTGAACGGAACCAAGAATGTGATTCAGGCAGCGGCGGAAGCCAAGGTGAGACGGGTGGTTTTCACGTCGTCAATCGGTGCGGTGTACATGGACCCCACCAGGGGTCCTGATGTGGTGGTTGATGAGTCGTGCTGGATCGATCTTGAATTTTGCAAGAACACCAAGAACTGGTACTGCTATGGGAAAGCAGTGGCAGAGCAGGCAGCATGGGCTGAGGCCAAAGAGAAAGGGGTGGACTTGGTGGTGGTGAACCCAGTGTTGGTGCTAGGACCACTTTTGCAACCAACTGTCAACGCTAGCATCATTCACATCCTCAAGTACCTGACAGGCTCAACCCAGACATATGCTAATTCAGTTCAGGCCTATGTGCATGTTAGGGACGTCGCACTGGCTCACATTCTGGTCTATGAGACACCATCGGCCTCTGGCAGATATCTTTGTGCTGAGAGTGTGCTCCACCGGGGTGACGTGGTGGAAATCCTGGCAAAATTCTTCCCGGAATACCCTATTCCCAACAAGTGTAAAGATAATGGAAAACCCAGGGCAGAACCATACAAGTTCACAAACCAGAAGCTACGAGACTTAGGGTTGGAGTTCACCCCAGTCAAACATACCCTGTATGAAACTGTCAAGAGCTTGCAGGAGAAGGGCCACCTTCCAGTCCCTACAAAACAAGAACAAGATTCCATTAAAATTCAATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006725 GO:0006950 GO:0007623 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009409 GO:0009628 GO:0009698 GO:0009699 GO:0009808 GO:0009809 GO:0009987 GO:0016491 GO:0016620 GO:0016621 GO:0016903 GO:0019438 GO:0019748 GO:0042752 GO:0042754 GO:0044237 GO:0044249 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0048511 GO:0048519 GO:0050789 GO:0050896 GO:0055114 GO:0065007 GO:0071704 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00940 ko01100 ko01110 map00940 map01100 map01110

Pfam Domains

Protein Families

Epimerase

Protein Analysis

340

Amino Acids

37.25

Weight (kDa)

6.16

Isoelectric Point (pI)

32.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NmrA	PF05368	13 - 132	2.4e-14	NmrA-like family
KR	PF08659	14 - 137	2e-06	KR domain
Epimerase	PF01370	15 - 247	6.9e-30	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	15 - 135	6.8e-08	Polysaccharide biosynthesis protein
3Beta_HSD	PF01073	17 - 240	1.6e-22	3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd	PF16363	17 - 264	3.9e-14	GDP-mannose 4,6 dehydratase
NAD_binding_4	PF07993	17 - 199	1.1e-11	Male sterility protein
NAD_binding_10	PF13460	19 - 134	5.3e-19	NAD(P)H-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002169)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15950 AT1G15950 AT1G15950 AT1G15950 AT1G15950 AT1G15950 AT1G15950 AT1G80820 AT1G80820
fragaria_vesca	FvH4_6g28680 FvH4_6g28680
malus_domestica	MD09G1224200.v1.1 MD17G1222400.v1.1
prunus_persica	Prupe.3G097500_v2.0.a1 Prupe.3G097600_v2.0.a1 Prupe.3G097700_v2.0.a1
pyrus_communis	pycom09g14320 pycom17g22630 pycom17g22650 pycom17g22670
rosa_chinensis	RchiOBHm_Chr1g0339261 RchiOBHm_Chr2g0133081
rosa_laevigata	RLG00000019307 RLG00000029205 RLG00000029212 RLG00000029219 RLG00000029221
rosa_multiflora	Rmu_sc0002571.1_g000033 Rmu_sc0033650.1_g000001 Rmu_sc0034151.1_g000001 Rmu_sc0034152.1_g000001
rosa_roxburghii	Rroxscaffold_2G00111530
rosa_rugosa	Rorug01G0143600.1 Rorug02G0309600
rosa_samantha	Rh1BG127100 Rh1CG149600 Rh1DG163700 Rh2AG362000 Rh2BG367700 Rh2CG345300 Rh2DG384600
rosa_wichuraiana	Rw1G013480 Rw2G029430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	699
AccB7I	CCANNNNNTGG	1 cut(s)	483
AciI	CCGC	4 cut(s)	312, 347, 350, 395
AclWI	GGATC	2 cut(s)	284, 458
AcoI	YGGCCR	2 cut(s)	28, 34
AcsI	RAATTY	3 cut(s)	461, 803, 1008
AcyI	GRCGYC	1 cut(s)	696
AfaI	GTAC	3 cut(s)	401, 488, 635
AfiI	CCNNNNNNNGG	4 cut(s)	415, 416, 483, 574
AgsI	TTSAA	3 cut(s)	173, 461, 1013
AjiI	CACGTC	2 cut(s)	381, 784
AjnI	CCWGG	3 cut(s)	413, 795, 854
AjuI	GAANNNNNNNTTGG	2 cut(s)	557, 589
AleI	CACNNNNGTG	1 cut(s)	777
AluBI	AGCT	9 cut(s)	15, 59, 91, 169, 179, 206, 239, 889, 951
AluI	AGCT	9 cut(s)	15, 59, 91, 169, 179, 206, 239, 889, 951
Alw21I	GWGCWC	1 cut(s)	771
Alw26I	GTCTC	4 cut(s)	181, 358, 719, 888
AlwI	GGATC	2 cut(s)	284, 458
AlwNI	CAGNNNCTG	1 cut(s)	26
AoxI	GGCC	6 cut(s)	28, 34, 531, 675, 735, 964
ApeKI	GCWGC	3 cut(s)	179, 344, 518
ApoI	RAATTY	3 cut(s)	461, 803, 1008
AspS9I	GGNCC	4 cut(s)	406, 419, 584, 964
AsuC2I	CCSGG	2 cut(s)	776, 812
AsuHPI	GGTGA	7 cut(s)	43, 97, 267, 373, 574, 791, 904
AsuNHI	GCTAGC	1 cut(s)	611
AvaII	GGWCC	3 cut(s)	406, 419, 584
BalI	TGGCCA	1 cut(s)	30
BanII	GRGCYC	1 cut(s)	312
Bbv12I	GWGCWC	1 cut(s)	771
BbvCI	CCTCAGC	1 cut(s)	528
BbvI	GCAGC	3 cut(s)	166, 356, 530
BccI	CCATC	3 cut(s)	76, 248, 739
BceAI	ACGGC	2 cut(s)	49, 262
BciT130I	CCWGG	3 cut(s)	415, 797, 856
BcnI	CCSGG	2 cut(s)	776, 812
BcoDI	GTCTC	4 cut(s)	181, 358, 719, 888
BfaI	CTAG	2 cut(s)	581, 612
BfmI	CTRYAG	1 cut(s)	180
BisI	GCNGC	4 cut(s)	180, 345, 348, 519
BlsI	GCNGC	4 cut(s)	181, 346, 349, 520
Bme1390I	CCNGG	5 cut(s)	415, 776, 797, 812, 856
Bme18I	GGWCC	3 cut(s)	406, 419, 584
BmgBI	CACGTC	2 cut(s)	381, 784
BmgT120I	GGNCC	4 cut(s)	406, 419, 584, 964
BmiI	GGNNCC	5 cut(s)	124, 309, 325, 408, 420
BmrFI	CCNGG	5 cut(s)	415, 776, 797, 812, 856
BmrI	ACTGGG	2 cut(s)	563, 911
BmsI	GCATC	2 cut(s)	281, 624
BmtI	GCTAGC	1 cut(s)	615
BmuI	ACTGGG	2 cut(s)	563, 911
BplI	GAGNNNNNCTC	2 cut(s)	195, 227
BpmI	CTGGAG	1 cut(s)	81
Bpu10I	CCTNAGC	1 cut(s)	528
BpuEI	CTTGAG	2 cut(s)	148, 614
BpuMI	CCSGG	2 cut(s)	776, 812
Bsa29I	ATCGAT	1 cut(s)	453
BsaHI	GRCGYC	1 cut(s)	696
BsaJI	CCNNGG	6 cut(s)	31, 357, 414, 775, 854, 855
BsaWI	WCCGGW	1 cut(s)	53
Bsc4I	CCNNNNNNNGG	4 cut(s)	415, 416, 483, 574
Bse1I	ACTGG	5 cut(s)	488, 569, 708, 917, 974
Bse3DI	GCAATG	1 cut(s)	69
BseBI	CCWGG	3 cut(s)	415, 797, 856
BseCI	ATCGAT	1 cut(s)	453
BseDI	CCNNGG	6 cut(s)	31, 357, 414, 775, 854, 855
BseGI	GGATG	3 cut(s)	87, 292, 624
BseLI	CCNNNNNNNGG	4 cut(s)	415, 416, 483, 574
BseMI	GCAATG	1 cut(s)	69
BseMII	CTCAG	2 cut(s)	519, 750
BseNI	ACTGG	5 cut(s)	488, 569, 708, 917, 974
BseXI	GCAGC	3 cut(s)	166, 356, 530
BsgI	GTGCAG	1 cut(s)	220
BshFI	GGCC	6 cut(s)	30, 36, 533, 677, 737, 966
BshVI	ATCGAT	1 cut(s)	453
BsiHKAI	GWGCWC	1 cut(s)	771
BsiSI	CCGG	3 cut(s)	54, 775, 812
BslFI	GGGAC	2 cut(s)	707, 962
BslI	CCNNNNNNNGG	4 cut(s)	415, 416, 483, 574
BsmAI	GTCTC	4 cut(s)	181, 358, 719, 888
BsmBI	CGTCTC	1 cut(s)	358
BsmFI	GGGAC	2 cut(s)	707, 962
BsmI	GAATGC	1 cut(s)	157
BsnI	GGCC	6 cut(s)	30, 36, 533, 677, 737, 966
Bsp1286I	GDGCHC	2 cut(s)	312, 771
Bsp1407I	TGTACA	1 cut(s)	399
Bsp143I	GATC	4 cut(s)	208, 289, 450, 454
BspACI	CCGC	4 cut(s)	312, 347, 350, 395
BspANI	GGCC	6 cut(s)	30, 36, 533, 677, 737, 966
BspCNI	CTCAG	2 cut(s)	520, 751
BspDI	ATCGAT	1 cut(s)	453
BspLI	GGNNCC	5 cut(s)	124, 309, 325, 408, 420
BspMAI	CTGCAG	1 cut(s)	184
BspOI	GCTAGC	1 cut(s)	615
BspPI	GGATC	2 cut(s)	284, 458
BsrDI	GCAATG	1 cut(s)	69
BsrGI	TGTACA	1 cut(s)	399
BsrI	ACTGG	5 cut(s)	488, 569, 708, 917, 974
BssECI	CCNNGG	6 cut(s)	31, 357, 414, 775, 854, 855
BssMI	GATC	4 cut(s)	208, 289, 450, 454
BssNI	GRCGYC	1 cut(s)	696
BssT1I	CCWWGG	1 cut(s)	357
Bst2UI	CCWGG	3 cut(s)	415, 797, 856
Bst4CI	ACNGT	3 cut(s)	115, 604, 943
BstACI	GRCGYC	1 cut(s)	696
BstAUI	TGTACA	1 cut(s)	399
BstC8I	GCNNGC	5 cut(s)	6, 312, 516, 613, 953
BstDEI	CTNAG	3 cut(s)	528, 759, 898
BstDSI	CCRYGG	1 cut(s)	31
BstF5I	GGATG	3 cut(s)	87, 292, 624
BstKTI	GATC	4 cut(s)	211, 292, 453, 457
BstMAI	GTCTC	4 cut(s)	181, 358, 719, 888
BstMBI	GATC	4 cut(s)	208, 289, 450, 454
BstMWI	GCNNNNNNNGC	3 cut(s)	353, 515, 524
BstNI	CCWGG	3 cut(s)	415, 797, 856
BstNSI	RCATGY	1 cut(s)	689
BstSCI	CCNGG	5 cut(s)	413, 774, 795, 810, 854
BstSFI	CTRYAG	1 cut(s)	180
BstV1I	GCAGC	3 cut(s)	166, 356, 530
Bsu15I	ATCGAT	1 cut(s)	453
BsuRI	GGCC	6 cut(s)	30, 36, 533, 677, 737, 966
BsuTUI	ATCGAT	1 cut(s)	453
BtgI	CCRYGG	1 cut(s)	31
BtrI	CACGTC	2 cut(s)	381, 784
BtsCI	GGATG	3 cut(s)	87, 292, 624
BtsI	GCAGTG	2 cut(s)	321, 510
BtsIMutI	CAGTG	5 cut(s)	111, 321, 510, 576, 701
Cac8I	GCNNGC	5 cut(s)	6, 312, 516, 613, 953
CaiI	CAGNNNCTG	1 cut(s)	26
Cfr13I	GGNCC	4 cut(s)	406, 419, 584, 964
ClaI	ATCGAT	1 cut(s)	453
Csp6I	GTAC	3 cut(s)	400, 487, 634
CviAII	CATG	3 cut(s)	403, 522, 686
CviQI	GTAC	3 cut(s)	400, 487, 634
DdeI	CTNAG	3 cut(s)	528, 759, 898
DpnI	GATC	4 cut(s)	210, 291, 452, 456
DpnII	GATC	4 cut(s)	208, 289, 450, 454
EaeI	YGGCCR	2 cut(s)	28, 34
EciI	GGCGGA	1 cut(s)	365
Eco130I	CCWWGG	1 cut(s)	357
Eco147I	AGGCCT	1 cut(s)	677
Eco24I	GRGCYC	1 cut(s)	312
Eco32I	GATATC	1 cut(s)	749
Eco47I	GGWCC	3 cut(s)	406, 419, 584
EcoO109I	RGGNCCY	1 cut(s)	419
EcoRII	CCWGG	3 cut(s)	413, 795, 854
EcoRV	GATATC	1 cut(s)	749
EcoT14I	CCWWGG	1 cut(s)	357
EcoT38I	GRGCYC	1 cut(s)	312
ErhI	CCWWGG	1 cut(s)	357
Esp3I	CGTCTC	1 cut(s)	358
FaeI	CATG	3 cut(s)	406, 525, 689
FaqI	GGGAC	2 cut(s)	707, 962
FatI	CATG	3 cut(s)	402, 521, 685
FauI	CCCGC	1 cut(s)	319
FauNDI	CATATG	1 cut(s)	658
Fnu4HI	GCNGC	4 cut(s)	180, 345, 348, 519
FokI	GGATG	3 cut(s)	94, 299, 611
FriOI	GRGCYC	1 cut(s)	312
Fsp4HI	GCNGC	4 cut(s)	180, 345, 348, 519
FspBI	CTAG	2 cut(s)	581, 612
GluI	GCNGC	4 cut(s)	180, 345, 348, 519
GsuI	CTGGAG	1 cut(s)	81
HaeIII	GGCC	6 cut(s)	30, 36, 533, 677, 737, 966
HapII	CCGG	3 cut(s)	54, 775, 812
Hin1I	GRCGYC	1 cut(s)	696
Hin1II	CATG	3 cut(s)	406, 525, 689
HincII	GTYRAC	1 cut(s)	607
HindII	GTYRAC	1 cut(s)	607
HinfI	GANTC	3 cut(s)	337, 440, 998
HpaII	CCGG	3 cut(s)	54, 775, 812
HphI	GGTGA	7 cut(s)	43, 97, 267, 373, 574, 791, 904
Hpy166II	GTNNAC	7 cut(s)	319, 400, 550, 565, 607, 876, 912
Hpy188III	TCNNGA	4 cut(s)	293, 422, 458, 946
Hpy8I	GTNNAC	7 cut(s)	319, 400, 550, 565, 607, 876, 912
Hpy99I	CGWCG	2 cut(s)	385, 701
HpyAV	CCTTC	3 cut(s)	167, 955, 980
HpyCH4III	ACNGT	3 cut(s)	115, 604, 943
HpyCH4IV	ACGT	3 cut(s)	380, 696, 783
HpyCH4V	TGCA	6 cut(s)	182, 201, 468, 595, 685, 955
HpyF10VI	GCNNNNNNNGC	3 cut(s)	353, 515, 524
HpyF3I	CTNAG	3 cut(s)	528, 759, 898
HpySE526I	ACGT	3 cut(s)	380, 696, 783
Hsp92I	GRCGYC	1 cut(s)	696
Hsp92II	CATG	3 cut(s)	406, 525, 689
Kzo9I	GATC	4 cut(s)	208, 289, 450, 454
LmnI	GCTCC	4 cut(s)	56, 176, 307, 774
Lsp1109I	GCAGC	3 cut(s)	166, 356, 530
LweI	GCATC	2 cut(s)	281, 624
MaeI	CTAG	2 cut(s)	581, 612
MaeII	ACGT	3 cut(s)	380, 696, 783
MaeIII	GTNAC	3 cut(s)	49, 280, 779
MalI	GATC	4 cut(s)	210, 291, 452, 456
MboI	GATC	4 cut(s)	208, 289, 450, 454
MboII	GAAGA	1 cut(s)	799
MhlI	GDGCHC	2 cut(s)	312, 771
MlsI	TGGCCA	1 cut(s)	30
MluCI	AATT	4 cut(s)	461, 664, 803, 1008
MluNI	TGGCCA	1 cut(s)	30
MlyI	GAGTC	1 cut(s)	449
MmeI	TCCRAC	1 cut(s)	885
MnlI	CCTC	7 cut(s)	56, 98, 113, 239, 523, 638, 748
Mox20I	TGGCCA	1 cut(s)	30
MscI	TGGCCA	1 cut(s)	30
MseI	TTAA	3 cut(s)	243, 1005, 1018
MslI	CAYNNNNRTG	1 cut(s)	777
Msp20I	TGGCCA	1 cut(s)	30
MspA1I	CMGCKG	1 cut(s)	347
MspI	CCGG	3 cut(s)	54, 775, 812
MspR9I	CCNGG	5 cut(s)	415, 776, 797, 812, 856
Mva1269I	GAATGC	1 cut(s)	157
MvaI	CCWGG	3 cut(s)	415, 797, 856
MwoI	GCNNNNNNNGC	3 cut(s)	353, 515, 524
NciI	CCSGG	2 cut(s)	776, 812
NdeI	CATATG	1 cut(s)	658
NdeII	GATC	4 cut(s)	208, 289, 450, 454
NheI	GCTAGC	1 cut(s)	611
NlaIII	CATG	3 cut(s)	406, 525, 689
NlaIV	GGNNCC	5 cut(s)	124, 309, 325, 408, 420
NmuCI	GTSAC	3 cut(s)	49, 280, 779
NspI	RCATGY	1 cut(s)	689
OliI	CACNNNNGTG	1 cut(s)	777
PasI	CCCWGGG	1 cut(s)	855
PceI	AGGCCT	1 cut(s)	677
PctI	GAATGC	1 cut(s)	157
PfeI	GAWTC	2 cut(s)	337, 998
PflMI	CCANNNNNTGG	1 cut(s)	483
PfoI	TCCNGGA	1 cut(s)	810
PkrI	GCNGC	4 cut(s)	181, 346, 349, 520
PleI	GAGTC	1 cut(s)	448
PpsI	GAGTC	1 cut(s)	448
PpuMI	RGGWCCY	1 cut(s)	419
Psp5II	RGGWCCY	1 cut(s)	419
Psp6I	CCWGG	3 cut(s)	413, 795, 854
PspGI	CCWGG	3 cut(s)	413, 795, 854
PspN4I	GGNNCC	5 cut(s)	124, 309, 325, 408, 420
PspPI	GGNCC	4 cut(s)	406, 419, 584, 964
PspPPI	RGGWCCY	1 cut(s)	419
PstI	CTGCAG	1 cut(s)	184
PstNI	CAGNNNCTG	1 cut(s)	26
RsaI	GTAC	3 cut(s)	401, 488, 635
RsaNI	GTAC	3 cut(s)	400, 487, 634
RseI	CAYNNNNRTG	1 cut(s)	777
SaqAI	TTAA	3 cut(s)	243, 1005, 1018
SatI	GCNGC	4 cut(s)	180, 345, 348, 519
Sau3AI	GATC	4 cut(s)	208, 289, 450, 454
Sau96I	GGNCC	4 cut(s)	406, 419, 584, 964
SchI	GAGTC	1 cut(s)	449
ScrFI	CCNGG	5 cut(s)	415, 776, 797, 812, 856
SduI	GDGCHC	2 cut(s)	312, 771
SfaNI	GCATC	2 cut(s)	281, 624
SfcI	CTRYAG	1 cut(s)	180
SinI	GGWCC	3 cut(s)	406, 419, 584
SmiMI	CAYNNNNRTG	1 cut(s)	777
SmlI	CTYRAG	2 cut(s)	127, 629
SmoI	CTYRAG	2 cut(s)	127, 629
Sse9I	AATT	4 cut(s)	461, 664, 803, 1008
SseBI	AGGCCT	1 cut(s)	677
SsiI	CCGC	4 cut(s)	312, 347, 350, 395
SspMI	CTAG	2 cut(s)	581, 612
StuI	AGGCCT	1 cut(s)	677
StyD4I	CCNGG	5 cut(s)	413, 774, 795, 810, 854
StyI	CCWWGG	1 cut(s)	357
TaaI	ACNGT	3 cut(s)	115, 604, 943
TaiI	ACGT	3 cut(s)	383, 699, 786
TaqI	TCGA	2 cut(s)	99, 453
TasI	AATT	4 cut(s)	461, 664, 803, 1008
TatI	WGTACW	1 cut(s)	399
TauI	GCSGC	1 cut(s)	350
TfiI	GAWTC	2 cut(s)	337, 998
Tru1I	TTAA	3 cut(s)	243, 1005, 1018
Tru9I	TTAA	3 cut(s)	243, 1005, 1018
TscAI	CASTG	5 cut(s)	118, 321, 510, 576, 708
TseFI	GTSAC	3 cut(s)	49, 280, 779
TseI	GCWGC	3 cut(s)	179, 344, 518
Tsp45I	GTSAC	3 cut(s)	49, 280, 779
TspDTI	ATGAA	2 cut(s)	58, 951
TspGWI	ACGGA	2 cut(s)	299, 336
TspRI	CASTG	5 cut(s)	118, 321, 510, 576, 708
Van91I	CCANNNNNTGG	1 cut(s)	483
VpaK11BI	GGWCC	3 cut(s)	406, 419, 584
XapI	RAATTY	3 cut(s)	461, 803, 1008
XceI	RCATGY	1 cut(s)	689
XspI	CTAG	2 cut(s)	581, 612
ZraI	GACGTC	1 cut(s)	697

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.