pycom420g01210

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0001790
Physical Location & Seq
Reverse (-)
1688608 .. 1689857
1250 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 999 bp
ATGGCCTTAGAGGAGGAAGTAGATAGCTTGTTCGAAAGCGCCATGAAGGGGCAGTGGGGAAAGGTGGTGGAAGCCTACAGAAACAGTTCCAAGGCTCAAGAGGCCAAGATAACCAAATCTAACGAGACTGCTCTCCACATTGCAATTGCAGATGGCCAAACAGAAATCGCAATCAAGTTGCTCAACATCATTTCCCTCGGAGGAAATGCATCCAAAATATTCAACTTAGGAAACGAGAAAGGCAACACAGCACTCCATCTGGCTGCCAGGCTTGGTTACGTGGAACTTTGCAAAATCATGGCTACGAAGGATAAAAACCTTGTCTCCGTTCGCAACCTGGACGGTGAGACCCCTCTCTTCTTGGCAGCTCATAATGGCAACATAAAGTCTTTCCTTTGCCTTCATTCTCACTGCCAAGAAAAGTTTCATTCCTTTAGAAACAACAATGGTGATACCATTCTCCATGCTGCTATCTCTGGTGAATACTTCTGTTTGGCGTTTCAGATAATTCGATTGTACCCGGAACTTGCTAACTCTATGAATGAAAACGGTTTATCTCCGCTGCATATTCTGGCCGGAAAGCCTAATGCATTCAAAAGCAGTACTCGCCTTGGACTAGTTGATGAGATTATACACCGCTGTTTGATCGTTGATGATCTCAAAATTGAAGCATATAATCATGAAGCTTGTCTGCACAAGGCAGGAGATCAAAACAGTTCCAAGCATCCGGCAAACTACGAAACGTGCATGAACTTCTTCCGGCTTTTGAGAAGTCTGTTCAAAGTGTTAAGTGGGTCTAATTCAGTTTCTCAAATTGTAGCGAAATCACGGGGAAATAAGAATTGTACCCAAGCTTTGCGCTTATACTGGAATAAACTGCGCGGGTCACTCCCTCAAGCTAATGGAGGAAACAGAAACAGAAATGGTGTCAATGCAGCTGAAGATGAAGAGAATCCTCGACCAGTGATCAGCTCAGTAATAGGTAAGCATCCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001508 GO:0002027 GO:0003008 GO:0003012 GO:0003013 GO:0003015 GO:0003205 GO:0003230 GO:0003279 GO:0003283 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006873 GO:0006874 GO:0006875 GO:0006888 GO:0006928 GO:0006936 GO:0006937 GO:0006941 GO:0006942 GO:0007009 GO:0007154 GO:0007165 GO:0007267 GO:0007275 GO:0007507 GO:0008015 GO:0008016 GO:0008092 GO:0008104 GO:0008150 GO:0009893 GO:0009987 GO:0010256 GO:0010468 GO:0010522 GO:0010604 GO:0010628 GO:0010646 GO:0010880 GO:0010881 GO:0010882 GO:0010959 GO:0014704 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0019222 GO:0019722 GO:0019725 GO:0019899 GO:0019900 GO:0019901 GO:0019932 GO:0022898 GO:0023051 GO:0023052 GO:0030001 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030048 GO:0030054 GO:0030154 GO:0030315 GO:0030507 GO:0030674 GO:0031430 GO:0031647 GO:0031672 GO:0031674 GO:0032409 GO:0032411 GO:0032412 GO:0032414 GO:0032501 GO:0032502 GO:0032879 GO:0032970 GO:0033036 GO:0033292 GO:0033365 GO:0034394 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0035556 GO:0035637 GO:0036309 GO:0036371 GO:0042383 GO:0042391 GO:0042592 GO:0042692 GO:0043034 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043266 GO:0043268 GO:0043269 GO:0043270 GO:0043292 GO:0044057 GO:0044093 GO:0044291 GO:0044325 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044449 GO:0044459 GO:0044464 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048731 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050821 GO:0050896 GO:0051049 GO:0051050 GO:0051117 GO:0051179 GO:0051234 GO:0051239 GO:0051270 GO:0051279 GO:0051282 GO:0051641 GO:0051649 GO:0051716 GO:0051899 GO:0051924 GO:0051928 GO:0055001 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055117 GO:0060047 GO:0060048 GO:0060090 GO:0060255 GO:0060306 GO:0060307 GO:0060341 GO:0061024 GO:0061061 GO:0061337 GO:0065007 GO:0065008 GO:0065009 GO:0070252 GO:0070296 GO:0070727 GO:0070838 GO:0070972 GO:0071840 GO:0071944 GO:0072359 GO:0072503 GO:0072507 GO:0072511 GO:0072657 GO:0072659 GO:0086001 GO:0086002 GO:0086003 GO:0086004 GO:0086005 GO:0086010 GO:0086012 GO:0086014 GO:0086015 GO:0086018 GO:0086019 GO:0086026 GO:0086046 GO:0086065 GO:0086066 GO:0086070 GO:0086091 GO:0090257 GO:0098590 GO:0098771 GO:0098900 GO:0098901 GO:0098907 GO:0098910 GO:0099080 GO:0099081 GO:0099512 GO:0099623 GO:1901016 GO:1901018 GO:1901019 GO:1901021 GO:1901379 GO:1901381 GO:1903115 GO:1903169 GO:1903522 GO:1903779 GO:1904062 GO:1904064 GO:1904427 GO:1990778 GO:2001257 GO:2001259
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 PGG

Protein Analysis

333

Amino Acids

36.57

Weight (kDa)

8.82

Isoelectric Point (pI)

33.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 34 - 104 1.2e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 110 - 162 2.4e-06 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 882
AciI CCGC 3 cut(s) 560, 637, 882
AcoI YGGCCR 2 cut(s) 154, 573
AcuI CTGAAG 1 cut(s) 960
AfaI GTAC 3 cut(s) 518, 604, 847
AfiI CCNNNNNNNGG 1 cut(s) 48
AgsI TTSAA 4 cut(s) 223, 595, 668, 781
AhlI ACTAGT 1 cut(s) 616
AjnI CCWGG 2 cut(s) 266, 336
AjuI GAANNNNNNNTTGG 2 cut(s) 408, 440
AluBI AGCT 7 cut(s) 27, 368, 686, 854, 899, 938, 972
AluI AGCT 7 cut(s) 27, 368, 686, 854, 899, 938, 972
Alw26I GTCTC 3 cut(s) 119, 328, 341
AoxI GGCC 4 cut(s) 3, 102, 154, 573
ApeKI GCWGC 5 cut(s) 263, 365, 467, 562, 935
Asp700I GAANNNNTTC 3 cut(s) 85, 423, 755
AspLEI GCGC 3 cut(s) 41, 861, 882
AsuC2I CCSGG 1 cut(s) 521
AsuHPI GGTGA 3 cut(s) 356, 461, 491
AsuII TTCGAA 1 cut(s) 33
BalI TGGCCA 1 cut(s) 156
BbvI GCAGC 5 cut(s) 250, 377, 454, 549, 947
BccI CCATC 2 cut(s) 146, 264
BciT130I CCWGG 2 cut(s) 268, 338
BclI TGATCA 1 cut(s) 966
BcnI CCSGG 1 cut(s) 521
BcoDI GTCTC 3 cut(s) 119, 328, 341
BcuI ACTAGT 1 cut(s) 616
BfaI CTAG 1 cut(s) 617
BfmI CTRYAG 1 cut(s) 76
BfoI RGCGCY 1 cut(s) 42
BisI GCNGC 5 cut(s) 264, 366, 468, 563, 936
BlsI GCNGC 5 cut(s) 265, 367, 469, 564, 937
BmcAI AGTACT 1 cut(s) 604
Bme1390I CCNGG 3 cut(s) 268, 338, 521
BmrFI CCNGG 3 cut(s) 268, 338, 521
BmsI GCATC 2 cut(s) 218, 733
Bpu14I TTCGAA 1 cut(s) 33
BpuEI CTTGAG 2 cut(s) 81, 879
BpuMI CCSGG 1 cut(s) 521
BsaAI YACGTR 1 cut(s) 280
BsaI GGTCTC 1 cut(s) 341
BsaJI CCNNGG 3 cut(s) 90, 196, 610
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bsc4I CCNNNNNNNGG 1 cut(s) 48
Bse1I ACTGG 2 cut(s) 872, 962
Bse3DI GCAATG 1 cut(s) 138
BseBI CCWGG 2 cut(s) 268, 338
BseDI CCNNGG 3 cut(s) 90, 196, 610
BseGI GGATG 3 cut(s) 209, 724, 988
BseLI CCNNNNNNNGG 1 cut(s) 48
BseMI GCAATG 1 cut(s) 138
BseMII CTCAG 1 cut(s) 987
BseNI ACTGG 2 cut(s) 872, 962
BseRI GAGGAG 1 cut(s) 26
BseXI GCAGC 5 cut(s) 250, 377, 454, 549, 947
BsgI GTGCAG 1 cut(s) 677
Bsh1236I CGCG 1 cut(s) 882
BshFI GGCC 4 cut(s) 5, 104, 156, 575
BsiSI CCGG 4 cut(s) 521, 576, 728, 760
BslI CCNNNNNNNGG 1 cut(s) 48
BsmAI GTCTC 3 cut(s) 119, 328, 341
BsmI GAATGC 1 cut(s) 590
BsnI GGCC 4 cut(s) 5, 104, 156, 575
Bso31I GGTCTC 1 cut(s) 341
Bsp119I TTCGAA 1 cut(s) 33
Bsp143I GATC 4 cut(s) 645, 655, 706, 966
BspACI CCGC 3 cut(s) 560, 637, 882
BspANI GGCC 4 cut(s) 5, 104, 156, 575
BspCNI CTCAG 1 cut(s) 986
BspFNI CGCG 1 cut(s) 882
BspHI TCATGA 1 cut(s) 679
BspT104I TTCGAA 1 cut(s) 33
BspTNI GGTCTC 1 cut(s) 341
BsrDI GCAATG 1 cut(s) 138
BsrI ACTGG 2 cut(s) 872, 962
BssECI CCNNGG 3 cut(s) 90, 196, 610
BssMI GATC 4 cut(s) 645, 655, 706, 966
BssT1I CCWWGG 2 cut(s) 90, 610
Bst2UI CCWGG 2 cut(s) 268, 338
Bst4CI ACNGT 4 cut(s) 86, 344, 551, 716
Bst6I CTCTTC 2 cut(s) 362, 942
BstBAI YACGTR 1 cut(s) 280
BstBI TTCGAA 1 cut(s) 33
BstDEI CTNAG 3 cut(s) 7, 226, 973
BstF5I GGATG 3 cut(s) 209, 724, 988
BstFNI CGCG 1 cut(s) 882
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 3 cut(s) 41, 861, 882
BstKTI GATC 4 cut(s) 648, 658, 709, 969
BstMAI GTCTC 3 cut(s) 119, 328, 341
BstMBI GATC 4 cut(s) 645, 655, 706, 966
BstMWI GCNNNNNNNGC 2 cut(s) 101, 606
BstNI CCWGG 2 cut(s) 268, 338
BstSCI CCNGG 3 cut(s) 266, 336, 519
BstSFI CTRYAG 1 cut(s) 76
BstUI CGCG 1 cut(s) 882
BstV1I GCAGC 5 cut(s) 250, 377, 454, 549, 947
BsuRI GGCC 4 cut(s) 5, 104, 156, 575
BtsCI GGATG 3 cut(s) 209, 724, 988
BtsI GCAGTG 2 cut(s) 59, 409
BtsIMutI CAGTG 3 cut(s) 59, 409, 969
CciI TCATGA 1 cut(s) 679
CfoI GCGC 3 cut(s) 41, 861, 882
Csp6I GTAC 3 cut(s) 517, 603, 846
CviAII CATG 5 cut(s) 43, 298, 464, 680, 748
CviQI GTAC 3 cut(s) 517, 603, 846
DdeI CTNAG 3 cut(s) 7, 226, 973
DpnI GATC 4 cut(s) 647, 657, 708, 968
DpnII GATC 4 cut(s) 645, 655, 706, 966
EaeI YGGCCR 2 cut(s) 154, 573
Eam1104I CTCTTC 2 cut(s) 362, 942
EarI CTCTTC 2 cut(s) 362, 942
Eco130I CCWWGG 2 cut(s) 90, 610
Eco31I GGTCTC 1 cut(s) 341
Eco57I CTGAAG 1 cut(s) 960
EcoRII CCWGG 2 cut(s) 266, 336
EcoT14I CCWWGG 2 cut(s) 90, 610
EcoT22I ATGCAT 2 cut(s) 211, 592
ErhI CCWWGG 2 cut(s) 90, 610
FaeI CATG 5 cut(s) 46, 301, 467, 683, 751
FatI CATG 5 cut(s) 42, 297, 463, 679, 747
FauI CCCGC 1 cut(s) 875
FbaI TGATCA 1 cut(s) 966
Fnu4HI GCNGC 5 cut(s) 264, 366, 468, 563, 936
FokI GGATG 3 cut(s) 196, 711, 975
Fsp4HI GCNGC 5 cut(s) 264, 366, 468, 563, 936
FspBI CTAG 1 cut(s) 617
GlaI GCGC 3 cut(s) 40, 860, 881
GluI GCNGC 5 cut(s) 264, 366, 468, 563, 936
HaeII RGCGCY 1 cut(s) 42
HaeIII GGCC 4 cut(s) 5, 104, 156, 575
HapII CCGG 4 cut(s) 521, 576, 728, 760
HhaI GCGC 3 cut(s) 41, 861, 882
Hin1II CATG 5 cut(s) 46, 301, 467, 683, 751
Hin6I GCGC 3 cut(s) 39, 859, 880
HinP1I GCGC 3 cut(s) 39, 859, 880
HindIII AAGCTT 2 cut(s) 684, 852
HinfI GANTC 1 cut(s) 952
HpaII CCGG 4 cut(s) 521, 576, 728, 760
HphI GGTGA 3 cut(s) 356, 461, 491
Hpy188I TCNGA 2 cut(s) 200, 504
Hpy188III TCNNGA 2 cut(s) 98, 680
HpyAV CCTTC 3 cut(s) 40, 301, 410
HpyCH4III ACNGT 4 cut(s) 86, 344, 551, 716
HpyCH4IV ACGT 2 cut(s) 279, 743
HpyCH4V TGCA 9 cut(s) 143, 149, 209, 291, 565, 590, 694, 747, 935
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 606
HpyF3I CTNAG 3 cut(s) 7, 226, 973
HpySE526I ACGT 2 cut(s) 279, 743
Hsp92II CATG 5 cut(s) 46, 301, 467, 683, 751
HspAI GCGC 3 cut(s) 39, 859, 880
Ksp22I TGATCA 1 cut(s) 966
Kzo9I GATC 4 cut(s) 645, 655, 706, 966
Lsp1109I GCAGC 5 cut(s) 250, 377, 454, 549, 947
LweI GCATC 2 cut(s) 218, 733
MaeI CTAG 1 cut(s) 617
MaeII ACGT 2 cut(s) 279, 743
MaeIII GTNAC 2 cut(s) 275, 885
MalI GATC 4 cut(s) 647, 657, 708, 968
MboI GATC 4 cut(s) 645, 655, 706, 966
MboII GAAGA 4 cut(s) 349, 748, 953, 959
MfeI CAATTG 1 cut(s) 144
MlsI TGGCCA 1 cut(s) 156
MluCI AATT 6 cut(s) 144, 507, 663, 799, 813, 841
MluNI TGGCCA 1 cut(s) 156
MnlI CCTC 9 cut(s) 4, 7, 94, 194, 206, 363, 899, 903, 966
Mox20I TGGCCA 1 cut(s) 156
Mph1103I ATGCAT 2 cut(s) 211, 592
MroXI GAANNNNTTC 3 cut(s) 85, 423, 755
MscI TGGCCA 1 cut(s) 156
MseI TTAA 1 cut(s) 788
Msp20I TGGCCA 1 cut(s) 156
MspA1I CMGCKG 3 cut(s) 562, 639, 938
MspI CCGG 4 cut(s) 521, 576, 728, 760
MspR9I CCNGG 3 cut(s) 268, 338, 521
MunI CAATTG 1 cut(s) 144
Mva1269I GAATGC 1 cut(s) 590
MvaI CCWGG 2 cut(s) 268, 338
MvnI CGCG 1 cut(s) 882
MwoI GCNNNNNNNGC 2 cut(s) 101, 606
NciI CCSGG 1 cut(s) 521
NdeII GATC 4 cut(s) 645, 655, 706, 966
NlaIII CATG 5 cut(s) 46, 301, 467, 683, 751
NmuCI GTSAC 1 cut(s) 885
NsiI ATGCAT 2 cut(s) 211, 592
NspV TTCGAA 1 cut(s) 33
PagI TCATGA 1 cut(s) 679
PctI GAATGC 1 cut(s) 590
PdmI GAANNNNTTC 3 cut(s) 85, 423, 755
PfeI GAWTC 1 cut(s) 952
PkrI GCNGC 5 cut(s) 265, 367, 469, 564, 937
Ppu21I YACGTR 1 cut(s) 280
Psp6I CCWGG 2 cut(s) 266, 336
PspGI CCWGG 2 cut(s) 266, 336
PvuII CAGCTG 1 cut(s) 938
RsaI GTAC 3 cut(s) 518, 604, 847
RsaNI GTAC 3 cut(s) 517, 603, 846
SaqAI TTAA 1 cut(s) 788
SatI GCNGC 5 cut(s) 264, 366, 468, 563, 936
Sau3AI GATC 4 cut(s) 645, 655, 706, 966
ScaI AGTACT 1 cut(s) 604
ScrFI CCNGG 3 cut(s) 268, 338, 521
SfaNI GCATC 2 cut(s) 218, 733
SfcI CTRYAG 1 cut(s) 76
SfuI TTCGAA 1 cut(s) 33
SmlI CTYRAG 2 cut(s) 96, 894
SmoI CTYRAG 2 cut(s) 96, 894
SpeI ACTAGT 1 cut(s) 616
Sse9I AATT 6 cut(s) 144, 507, 663, 799, 813, 841
SsiI CCGC 3 cut(s) 560, 637, 882
SspI AATATT 1 cut(s) 219
SspMI CTAG 1 cut(s) 617
StyD4I CCNGG 3 cut(s) 266, 336, 519
StyI CCWWGG 2 cut(s) 90, 610
TaaI ACNGT 4 cut(s) 86, 344, 551, 716
TaiI ACGT 2 cut(s) 282, 746
TaqI TCGA 3 cut(s) 33, 511, 958
TasI AATT 6 cut(s) 144, 507, 663, 799, 813, 841
TatI WGTACW 1 cut(s) 602
TfiI GAWTC 1 cut(s) 952
Tru1I TTAA 1 cut(s) 788
Tru9I TTAA 1 cut(s) 788
TscAI CASTG 3 cut(s) 59, 416, 969
TseFI GTSAC 1 cut(s) 885
TseI GCWGC 5 cut(s) 263, 365, 467, 562, 935
Tsp45I GTSAC 1 cut(s) 885
TspDTI ATGAA 8 cut(s) 59, 392, 416, 554, 558, 696, 764, 960
TspGWI ACGGA 1 cut(s) 316
TspRI CASTG 3 cut(s) 59, 416, 969
XmnI GAANNNNTTC 3 cut(s) 85, 423, 755
XspI CTAG 1 cut(s) 617
ZrmI AGTACT 1 cut(s) 604
Zsp2I ATGCAT 2 cut(s) 211, 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.