RchiOBHm_Chr5g0019901

Protein PHLOEM PROTEIN 2-LIKE A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003915
Physical Location & Seq
Forward (+)
14294821 .. 14296227
1407 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 522 bp
ATGGCTATGACTAAACCTCATTGGCAAGCAGAGAAAGAAGAAATCATAAAGCAGGATGAGCAGGGAGATAACTACCGTATCAAACCACGAGGACTTAATATTGTATGGGGCAATGATGAACGCTACTGGAAAATACCAGCCAAAAGGACTGAGGATCCTGCAGAGCTGGTACAGGTCTCATGGCTGGAAGTAACAGGCTCAGTTGATGAGTTGAAACCCGGGAAGGGCTATGATCTGACCTTCGAGGTGGGGCTGGCAGGAGATGCATTTGGTTGGAAAGACATACAAGTGTTCTTGATGGCAAAGGTAGGGAAAAAGGGAAGGTACAAATGGACAAGAGTTAAACTCGATCAGGATAGGACTAACGAGAGAGTTACAATTCCTTATAACCAGAAGCTTAGCATTGATGTTCCAGCTGGTACAAGGGACACCACCCTTCATTTTGGTCTGTATGAAGTGTGGAGTGGGAAATGGAAGGGAGGCTTGAAGATTTATAATGCAAAAGTAACTGCGAGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

173

Amino Acids

19.87

Weight (kDa)

9.12

Isoelectric Point (pI)

11.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 24 - 167 8.9e-23 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 387, 495
AclWI GGATC 2 cut(s) 149, 162
AfaI GTAC 3 cut(s) 171, 326, 421
AfiI CCNNNNNNNGG 1 cut(s) 224
AgsI TTSAA 2 cut(s) 214, 487
AluBI AGCT 3 cut(s) 166, 397, 416
AluI AGCT 3 cut(s) 166, 397, 416
Alw26I GTCTC 1 cut(s) 181
AlwI GGATC 2 cut(s) 149, 162
Ama87I CYCGRG 1 cut(s) 218
AsuC2I CCSGG 2 cut(s) 219, 220
AvaI CYCGRG 1 cut(s) 218
BaeI ACNNNNGTAYC 2 cut(s) 61, 94
BamHI GGATCC 1 cut(s) 154
BauI CACGAG 1 cut(s) 87
BccI CCATC 1 cut(s) 292
BcnI CCSGG 2 cut(s) 219, 220
BcoDI GTCTC 1 cut(s) 181
BfmI CTRYAG 1 cut(s) 159
BlpI GCTNAGC 1 cut(s) 398
Bme1390I CCNGG 2 cut(s) 219, 220
BmeT110I CYCGRG 1 cut(s) 218
BmiI GGNNCC 1 cut(s) 156
BmrFI CCNGG 2 cut(s) 219, 220
BmsI GCATC 1 cut(s) 253
BplI GAGNNNNNCTC 2 cut(s) 330, 362
Bpu1102I GCTNAGC 1 cut(s) 398
BpuMI CCSGG 2 cut(s) 219, 220
BsaI GGTCTC 1 cut(s) 181
BsaJI CCNNGG 1 cut(s) 218
Bsc4I CCNNNNNNNGG 1 cut(s) 224
Bse1I ACTGG 1 cut(s) 131
Bse3DI GCAATG 1 cut(s) 118
BseDI CCNNGG 1 cut(s) 218
BseGI GGATG 1 cut(s) 61
BseLI CCNNNNNNNGG 1 cut(s) 224
BseMI GCAATG 1 cut(s) 118
BseMII CTCAG 2 cut(s) 141, 213
BseNI ACTGG 1 cut(s) 131
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 1 cut(s) 219
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 1 cut(s) 224
BsmAI GTCTC 1 cut(s) 181
BsmFI GGGAC 1 cut(s) 440
Bso31I GGTCTC 1 cut(s) 181
BsoBI CYCGRG 1 cut(s) 218
Bsp143I GATC 3 cut(s) 154, 232, 349
Bsp1720I GCTNAGC 1 cut(s) 398
BspCNI CTCAG 2 cut(s) 142, 212
BspLI GGNNCC 1 cut(s) 156
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 2 cut(s) 149, 162
BspTNI GGTCTC 1 cut(s) 181
BsrDI GCAATG 1 cut(s) 118
BsrI ACTGG 1 cut(s) 131
BssECI CCNNGG 1 cut(s) 218
BssMI GATC 3 cut(s) 154, 232, 349
BssSI CACGAG 1 cut(s) 87
Bst2BI CACGAG 1 cut(s) 87
Bst4CI ACNGT 1 cut(s) 77
BstAPI GCANNNNNTGC 1 cut(s) 263
BstC8I GCNNGC 2 cut(s) 27, 255
BstDEI CTNAG 3 cut(s) 150, 199, 398
BstF5I GGATG 1 cut(s) 61
BstKTI GATC 3 cut(s) 157, 235, 352
BstMAI GTCTC 1 cut(s) 181
BstMBI GATC 3 cut(s) 154, 232, 349
BstMWI GCNNNNNNNGC 2 cut(s) 58, 263
BstSCI CCNGG 2 cut(s) 217, 218
BstSFI CTRYAG 1 cut(s) 159
BstX2I RGATCY 1 cut(s) 154
BstYI RGATCY 1 cut(s) 154
BtsCI GGATG 1 cut(s) 61
Cac8I GCNNGC 2 cut(s) 27, 255
Cfr9I CCCGGG 1 cut(s) 218
Csp6I GTAC 3 cut(s) 170, 325, 420
CviAII CATG 1 cut(s) 180
CviQI GTAC 3 cut(s) 170, 325, 420
DdeI CTNAG 3 cut(s) 150, 199, 398
DpnI GATC 3 cut(s) 156, 234, 351
DpnII GATC 3 cut(s) 154, 232, 349
Eco31I GGTCTC 1 cut(s) 181
Eco88I CYCGRG 1 cut(s) 218
EcoT22I ATGCAT 1 cut(s) 268
FaeI CATG 1 cut(s) 183
FaiI YATR 9 cut(s) 8, 47, 106, 181, 231, 284, 387, 453, 495
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FaqI GGGAC 1 cut(s) 440
FatI CATG 1 cut(s) 179
FokI GGATG 1 cut(s) 68
HapII CCGG 1 cut(s) 219
Hin1II CATG 1 cut(s) 183
HindIII AAGCTT 1 cut(s) 395
HinfI GANTC 1 cut(s) 514
HpaII CCGG 1 cut(s) 219
Hpy188I TCNGA 1 cut(s) 237
Hpy188III TCNNGA 2 cut(s) 295, 353
HpyAV CCTTC 5 cut(s) 217, 250, 315, 446, 469
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4V TGCA 3 cut(s) 161, 266, 500
HpyF10VI GCNNNNNNNGC 2 cut(s) 58, 263
HpyF3I CTNAG 3 cut(s) 150, 199, 398
Hsp92II CATG 1 cut(s) 183
Kzo9I GATC 3 cut(s) 154, 232, 349
LweI GCATC 1 cut(s) 253
MaeIII GTNAC 3 cut(s) 190, 373, 505
MalI GATC 3 cut(s) 156, 234, 351
MboI GATC 3 cut(s) 154, 232, 349
MboII GAAGA 2 cut(s) 50, 499
MflI RGATCY 1 cut(s) 154
MluCI AATT 1 cut(s) 378
MmeI TCCRAC 1 cut(s) 254
MnlI CCTC 5 cut(s) 27, 83, 145, 238, 473
Mph1103I ATGCAT 1 cut(s) 268
MseI TTAA 2 cut(s) 96, 342
MslI CAYNNNNRTG 1 cut(s) 287
MspA1I CMGCKG 1 cut(s) 416
MspI CCGG 1 cut(s) 219
MspR9I CCNGG 2 cut(s) 219, 220
MwoI GCNNNNNNNGC 2 cut(s) 58, 263
NciI CCSGG 2 cut(s) 219, 220
NdeII GATC 3 cut(s) 154, 232, 349
NlaIII CATG 1 cut(s) 183
NlaIV GGNNCC 1 cut(s) 156
NsiI ATGCAT 1 cut(s) 268
PsiI TTATAA 2 cut(s) 387, 495
PspN4I GGNNCC 1 cut(s) 156
PstI CTGCAG 1 cut(s) 163
PsuI RGATCY 1 cut(s) 154
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 3 cut(s) 171, 326, 421
RsaNI GTAC 3 cut(s) 170, 325, 420
RseI CAYNNNNRTG 1 cut(s) 287
SaqAI TTAA 2 cut(s) 96, 342
Sau3AI GATC 3 cut(s) 154, 232, 349
ScrFI CCNGG 2 cut(s) 219, 220
SetI ASST 9 cut(s) 19, 168, 177, 242, 249, 309, 326, 399, 418
SfaNI GCATC 1 cut(s) 253
SfcI CTRYAG 1 cut(s) 159
SmaI CCCGGG 1 cut(s) 220
SmiMI CAYNNNNRTG 1 cut(s) 287
Sse9I AATT 1 cut(s) 378
SspI AATATT 1 cut(s) 100
StyD4I CCNGG 2 cut(s) 217, 218
TaaI ACNGT 1 cut(s) 77
TaqI TCGA 2 cut(s) 243, 348
TasI AATT 1 cut(s) 378
Tru1I TTAA 2 cut(s) 96, 342
Tru9I TTAA 2 cut(s) 96, 342
TspDTI ATGAA 3 cut(s) 132, 428, 468
TspMI CCCGGG 1 cut(s) 218
XmaI CCCGGG 1 cut(s) 218
Zsp2I ATGCAT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.