RchiOBHm_Chr1g0362901

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001935
Physical Location & Seq
Forward (+)
54483695 .. 54484655
961 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 657 bp
ATGTTGCCTGAGCTGCCTAATGCTCTTGATCTAGAACCTAGATCACATCAGACTCAGTTCCAGCAAATCAGTTATTGGAAGAATAACCCTAACGTCATCGTGTCTAGGGATTGGACTGGAAATTTCCAAAAGATAATGCAGGCAGTAGAGGCTGTACCGGATCATAGCCGGAAGCGTCTTGTGATCTTGGTGAACAGAGGAGTTTACAAGGAAAATGTGGATATCAATGTGAAAAAGACTAATTTGGTTTTGATTGGAGAGGGCATGGATGTTGCGACCAGAACTGGACATAAGAGTAATGGTACTGGTTGGCGTACATTTACCTCGGCAACATTTACTGTGAAGGCAGAAGGCTTCATTGCAATCTATATAGGGTTTGAGAACACAGCCGGACCACACAACGGCCAGGCAGTGGCACTTCTAGTCAAGGGTAACCAATCAGCTTTCTTCCGATGCAAGATAAGTGGATACCAAGACACATTGTATGCACACAACAGCCGCCAGTTCTTTAGGGAATGCAAGATCAGTGGCACTGTCGACTTCATCTTCGGCTACAGCACTGCAGTTTTCCAAATGTGCACCAATATTGCGAAGAAAAGACCTAATCATGGCCAGTTACTGCCCACGGTCGTTTCGAAGAAGAATCAACCGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

218

Amino Acids

24.44

Weight (kDa)

9.74

Isoelectric Point (pI)

23.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 31 - 206 1.6e-68 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 412
AccI GTMKAC 1 cut(s) 537
AciI CCGC 1 cut(s) 499
AclWI GGATC 1 cut(s) 168
AcoI YGGCCR 2 cut(s) 403, 610
AcsI RAATTY 1 cut(s) 121
AfaI GTAC 3 cut(s) 156, 304, 316
AfiI CCNNNNNNNGG 3 cut(s) 401, 412, 608
AjnI CCWGG 1 cut(s) 405
AluBI AGCT 2 cut(s) 13, 443
AluI AGCT 2 cut(s) 13, 443
Alw21I GWGCWC 1 cut(s) 581
Alw44I GTGCAC 1 cut(s) 577
AlwI GGATC 1 cut(s) 168
AlwNI CAGNNNCTG 1 cut(s) 619
AoxI GGCC 2 cut(s) 403, 610
ApaLI GTGCAC 1 cut(s) 577
ApeKI GCWGC 1 cut(s) 13
ApoI RAATTY 1 cut(s) 121
ArsI GACNNNNNNTTYG 2 cut(s) 530, 562
AspS9I GGNCC 1 cut(s) 392
AsuC2I CCSGG 1 cut(s) 651
AsuHPI GGTGA 1 cut(s) 202
AsuII TTCGAA 1 cut(s) 635
AvaII GGWCC 1 cut(s) 392
BaeGI GKGCMC 1 cut(s) 581
BalI TGGCCA 1 cut(s) 612
Bbv12I GWGCWC 1 cut(s) 581
BceAI ACGGC 1 cut(s) 418
BciT130I CCWGG 1 cut(s) 407
BciVI GTATCC 1 cut(s) 461
BcnI CCSGG 1 cut(s) 651
BfaI CTAG 4 cut(s) 32, 39, 105, 422
BfmI CTRYAG 2 cut(s) 553, 561
BfuI GTATCC 1 cut(s) 461
BisI GCNGC 2 cut(s) 14, 499
BlsI GCNGC 2 cut(s) 15, 500
Bme1390I CCNGG 2 cut(s) 407, 651
Bme18I GGWCC 1 cut(s) 392
BmgT120I GGNCC 1 cut(s) 392
BmrFI CCNGG 2 cut(s) 407, 651
BmsI GCATC 1 cut(s) 443
Bpu10I CCTNAGC 1 cut(s) 9
Bpu14I TTCGAA 1 cut(s) 635
BpuMI CCSGG 1 cut(s) 651
BsaJI CCNNGG 2 cut(s) 324, 624
BsaWI WCCGGW 1 cut(s) 157
Bsc4I CCNNNNNNNGG 3 cut(s) 401, 412, 608
Bse1I ACTGG 5 cut(s) 121, 289, 310, 502, 613
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 1 cut(s) 407
BseDI CCNNGG 2 cut(s) 324, 624
BseGI GGATG 1 cut(s) 274
BseLI CCNNNNNNNGG 3 cut(s) 401, 412, 608
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 1 cut(s) 68
BseNI ACTGG 5 cut(s) 121, 289, 310, 502, 613
BseRI GAGGAG 1 cut(s) 213
BseSI GKGCMC 1 cut(s) 581
Bsh1285I CGRYCG 1 cut(s) 630
BshFI GGCC 2 cut(s) 405, 612
BsiEI CGRYCG 1 cut(s) 630
BsiHKAI GWGCWC 1 cut(s) 581
BsiSI CCGG 4 cut(s) 158, 169, 390, 650
BslI CCNNNNNNNGG 3 cut(s) 401, 412, 608
BsmI GAATGC 1 cut(s) 521
BsnI GGCC 2 cut(s) 405, 612
Bsp119I TTCGAA 1 cut(s) 635
Bsp1286I GDGCHC 1 cut(s) 581
Bsp143I GATC 5 cut(s) 28, 41, 160, 183, 522
BspACI CCGC 1 cut(s) 499
BspANI GGCC 2 cut(s) 405, 612
BspCNI CTCAG 1 cut(s) 67
BspMAI CTGCAG 1 cut(s) 565
BspPI GGATC 1 cut(s) 168
BspT104I TTCGAA 1 cut(s) 635
BsrDI GCAATG 1 cut(s) 357
BsrI ACTGG 5 cut(s) 121, 289, 310, 502, 613
BssECI CCNNGG 2 cut(s) 324, 624
BssMI GATC 5 cut(s) 28, 41, 160, 183, 522
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 3 cut(s) 340, 535, 628
BstBI TTCGAA 1 cut(s) 635
BstC8I GCNNGC 1 cut(s) 141
BstDEI CTNAG 2 cut(s) 9, 54
BstDSI CCRYGG 1 cut(s) 624
BstEII GGTNACC 1 cut(s) 431
BstF5I GGATG 1 cut(s) 274
BstKTI GATC 5 cut(s) 31, 44, 163, 186, 525
BstMBI GATC 5 cut(s) 28, 41, 160, 183, 522
BstMCI CGRYCG 1 cut(s) 630
BstMWI GCNNNNNNNGC 2 cut(s) 13, 149
BstNI CCWGG 1 cut(s) 407
BstPI GGTNACC 1 cut(s) 431
BstSCI CCNGG 2 cut(s) 405, 649
BstSFI CTRYAG 2 cut(s) 553, 561
BstSLI GKGCMC 1 cut(s) 581
BsuI GTATCC 1 cut(s) 461
BsuRI GGCC 2 cut(s) 405, 612
BtgI CCRYGG 1 cut(s) 624
BtsCI GGATG 1 cut(s) 274
BtsI GCAGTG 2 cut(s) 417, 558
BtsIMutI CAGTG 4 cut(s) 417, 531, 532, 558
Cac8I GCNNGC 1 cut(s) 141
CaiI CAGNNNCTG 1 cut(s) 619
Cfr13I GGNCC 1 cut(s) 392
CseI GACGC 1 cut(s) 164
Csp6I GTAC 3 cut(s) 155, 303, 315
CspCI CAANNNNNGTGG 4 cut(s) 445, 480, 508, 543
CviAII CATG 2 cut(s) 265, 608
CviQI GTAC 3 cut(s) 155, 303, 315
DdeI CTNAG 2 cut(s) 9, 54
DpnI GATC 5 cut(s) 30, 43, 162, 185, 524
DpnII GATC 5 cut(s) 28, 41, 160, 183, 522
EaeI YGGCCR 2 cut(s) 403, 610
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 392
Eco91I GGTNACC 1 cut(s) 431
EcoO65I GGTNACC 1 cut(s) 431
EcoRII CCWGG 1 cut(s) 405
EcoRV GATATC 1 cut(s) 223
FaeI CATG 2 cut(s) 268, 611
FaiI YATR 7 cut(s) 165, 266, 291, 369, 371, 486, 609
FatI CATG 2 cut(s) 264, 607
FblI GTMKAC 1 cut(s) 537
Fnu4HI GCNGC 2 cut(s) 14, 499
FokI GGATG 1 cut(s) 281
Fsp4HI GCNGC 2 cut(s) 14, 499
FspBI CTAG 4 cut(s) 32, 39, 105, 422
GluI GCNGC 2 cut(s) 14, 499
HaeIII GGCC 2 cut(s) 405, 612
HapII CCGG 4 cut(s) 158, 169, 390, 650
HgaI GACGC 1 cut(s) 164
Hin1II CATG 2 cut(s) 268, 611
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HinfI GANTC 2 cut(s) 52, 643
HpaII CCGG 4 cut(s) 158, 169, 390, 650
HphI GGTGA 1 cut(s) 202
Hpy166II GTNNAC 4 cut(s) 193, 205, 538, 579
Hpy188I TCNGA 2 cut(s) 51, 452
Hpy188III TCNNGA 2 cut(s) 26, 32
Hpy8I GTNNAC 4 cut(s) 193, 205, 538, 579
HpyAV CCTTC 2 cut(s) 337, 344
HpyCH4III ACNGT 3 cut(s) 340, 535, 628
HpyCH4IV ACGT 1 cut(s) 93
HpyCH4V TGCA 7 cut(s) 139, 362, 456, 488, 519, 563, 579
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 149
HpyF3I CTNAG 2 cut(s) 9, 54
HpySE526I ACGT 1 cut(s) 93
Hsp92II CATG 2 cut(s) 268, 611
Kzo9I GATC 5 cut(s) 28, 41, 160, 183, 522
LweI GCATC 1 cut(s) 443
MaeI CTAG 4 cut(s) 32, 39, 105, 422
MaeII ACGT 1 cut(s) 93
MaeIII GTNAC 2 cut(s) 431, 615
MalI GATC 5 cut(s) 30, 43, 162, 185, 524
MboI GATC 5 cut(s) 28, 41, 160, 183, 522
MboII GAAGA 6 cut(s) 91, 439, 538, 604, 649, 652
MhlI GDGCHC 1 cut(s) 581
MlsI TGGCCA 1 cut(s) 612
MluCI AATT 2 cut(s) 121, 241
MluNI TGGCCA 1 cut(s) 612
MlyI GAGTC 1 cut(s) 46
MnlI CCTC 4 cut(s) 142, 191, 253, 334
Mox20I TGGCCA 1 cut(s) 612
MscI TGGCCA 1 cut(s) 612
Msp20I TGGCCA 1 cut(s) 612
MspI CCGG 4 cut(s) 158, 169, 390, 650
MspR9I CCNGG 2 cut(s) 407, 651
Mva1269I GAATGC 1 cut(s) 521
MvaI CCWGG 1 cut(s) 407
MwoI GCNNNNNNNGC 2 cut(s) 13, 149
NciI CCSGG 1 cut(s) 651
NdeII GATC 5 cut(s) 28, 41, 160, 183, 522
NlaIII CATG 2 cut(s) 268, 611
NmeAIII GCCGAG 1 cut(s) 305
NspV TTCGAA 1 cut(s) 635
PcsI WCGNNNNNNNCGW 1 cut(s) 632
PctI GAATGC 1 cut(s) 521
PfeI GAWTC 1 cut(s) 643
PflMI CCANNNNNTGG 1 cut(s) 412
PkrI GCNGC 2 cut(s) 15, 500
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
Psp6I CCWGG 1 cut(s) 405
PspEI GGTNACC 1 cut(s) 431
PspGI CCWGG 1 cut(s) 405
PspPI GGNCC 1 cut(s) 392
PstI CTGCAG 1 cut(s) 565
PstNI CAGNNNCTG 1 cut(s) 619
RsaI GTAC 3 cut(s) 156, 304, 316
RsaNI GTAC 3 cut(s) 155, 303, 315
SalI GTCGAC 1 cut(s) 536
SatI GCNGC 2 cut(s) 14, 499
Sau3AI GATC 5 cut(s) 28, 41, 160, 183, 522
Sau96I GGNCC 1 cut(s) 392
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 2 cut(s) 407, 651
SduI GDGCHC 1 cut(s) 581
SetI ASST 6 cut(s) 15, 40, 96, 326, 445, 604
SfaNI GCATC 1 cut(s) 443
SfcI CTRYAG 2 cut(s) 553, 561
SfuI TTCGAA 1 cut(s) 635
SinI GGWCC 1 cut(s) 392
Sse9I AATT 2 cut(s) 121, 241
SsiI CCGC 1 cut(s) 499
SspI AATATT 1 cut(s) 586
SspMI CTAG 4 cut(s) 32, 39, 105, 422
StyD4I CCNGG 2 cut(s) 405, 649
TaaI ACNGT 3 cut(s) 340, 535, 628
TaiI ACGT 1 cut(s) 96
TaqI TCGA 2 cut(s) 537, 635
TasI AATT 2 cut(s) 121, 241
TauI GCSGC 1 cut(s) 501
TfiI GAWTC 1 cut(s) 643
TscAI CASTG 4 cut(s) 417, 532, 538, 565
TseI GCWGC 1 cut(s) 13
TspDTI ATGAA 2 cut(s) 346, 532
TspRI CASTG 4 cut(s) 417, 532, 538, 565
Van91I CCANNNNNTGG 1 cut(s) 412
VneI GTGCAC 1 cut(s) 577
VpaK11BI GGWCC 1 cut(s) 392
XapI RAATTY 1 cut(s) 121
XbaI TCTAGA 1 cut(s) 31
XmiI GTMKAC 1 cut(s) 537
XspI CTAG 4 cut(s) 32, 39, 105, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.