RchiOBHm_Chr1g0330511

Cytochrome b6-f complex iron-sulfur subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003668
Physical Location & Seq
Reverse (-)
20220025 .. 20220747
723 bp
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UTR
Exon/CDS
Intron
PRQ55970

Sequence Viewer

Length: 417 bp
ATGATGGGAAAGGGAAAGGGAAAGGGAATGAGAATAACATGCCAGGCCGCAATTCCAGCTGATAATGTCCCTGACATGGGTAAGAGGAACCTTATGAATCTGCTTCTTCTAACGAATCTCACTTCCCTCTGGTTTCCTATGCCACTTTCTTTGCTCCACCTGGGTGAGTTTGAATCATTGAACAATTATAGCTCTGGTGTTGGAACTGGTGGTCAAGTTACCAAGGATGCTCTAGGAAATGATGTAATTGCAGCAGAATGGCTTAAGACCCATGGCCCTGGTGATAGGACTTTCACACAAGGATTGAAGGTACTAATGCATCTTACTACTATTTCAATTTATTTGGAAAGTTCAAGTTCATTTTACAATGTTGTTAGTCCATGTGGCTTCCAGTTTTGGCCTTGGAGAGGATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
CytB6-F_Fe-S Rieske

Protein Analysis

138

Amino Acids

15.11

Weight (kDa)

8.64

Isoelectric Point (pI)

23.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TM_PetC PF25471 20 - 39 8e-07 Iron-sulfur subunit PetC transmembrane anchor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 415
AciI CCGC 1 cut(s) 48
AfaI GTAC 1 cut(s) 312
AfiI CCNNNNNNNGG 3 cut(s) 76, 77, 407
AflII CTTAAG 1 cut(s) 263
AgsI TTSAA 5 cut(s) 173, 181, 307, 336, 354
AjnI CCWGG 3 cut(s) 42, 159, 277
AleI CACNNNNGTG 1 cut(s) 162
AluBI AGCT 2 cut(s) 59, 192
AluI AGCT 2 cut(s) 59, 192
AoxI GGCC 3 cut(s) 45, 274, 398
ApeKI GCWGC 1 cut(s) 251
AspS9I GGNCC 1 cut(s) 275
AsuHPI GGTGA 2 cut(s) 176, 293
BbvI GCAGC 1 cut(s) 263
BciT130I CCWGG 3 cut(s) 44, 161, 279
BfaI CTAG 1 cut(s) 233
BfrI CTTAAG 1 cut(s) 263
BisI GCNGC 2 cut(s) 48, 252
BlsI GCNGC 2 cut(s) 49, 253
Bme1390I CCNGG 3 cut(s) 44, 161, 279
BmgT120I GGNCC 1 cut(s) 275
BmiI GGNNCC 1 cut(s) 89
BmrFI CCNGG 3 cut(s) 44, 161, 279
BmsI GCATC 2 cut(s) 217, 328
BsaJI CCNNGG 5 cut(s) 160, 222, 271, 277, 401
Bsc4I CCNNNNNNNGG 3 cut(s) 76, 77, 407
Bse1I ACTGG 2 cut(s) 211, 391
BseBI CCWGG 3 cut(s) 44, 161, 279
BseDI CCNNGG 5 cut(s) 160, 222, 271, 277, 401
BseGI GGATG 1 cut(s) 232
BseLI CCNNNNNNNGG 3 cut(s) 76, 77, 407
BseNI ACTGG 2 cut(s) 211, 391
BseXI GCAGC 1 cut(s) 263
BshFI GGCC 3 cut(s) 47, 276, 400
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 3 cut(s) 76, 77, 407
BsmFI GGGAC 1 cut(s) 53
BsnI GGCC 3 cut(s) 47, 276, 400
Bsp19I CCATGG 1 cut(s) 271
BspACI CCGC 1 cut(s) 48
BspANI GGCC 3 cut(s) 47, 276, 400
BspLI GGNNCC 1 cut(s) 89
BspTI CTTAAG 1 cut(s) 263
BsrI ACTGG 2 cut(s) 211, 391
BssECI CCNNGG 5 cut(s) 160, 222, 271, 277, 401
BssT1I CCWWGG 3 cut(s) 222, 271, 401
Bst2UI CCWGG 3 cut(s) 44, 161, 279
BstAFI CTTAAG 1 cut(s) 263
BstDSI CCRYGG 1 cut(s) 271
BstENI CCTNNNNNAGG 1 cut(s) 405
BstF5I GGATG 1 cut(s) 232
BstMWI GCNNNNNNNGC 1 cut(s) 56
BstNI CCWGG 3 cut(s) 44, 161, 279
BstNSI RCATGY 1 cut(s) 42
BstSCI CCNGG 3 cut(s) 42, 159, 277
BstV1I GCAGC 1 cut(s) 263
BstXI CCANNNNNNTGG 1 cut(s) 278
BsuRI GGCC 3 cut(s) 47, 276, 400
BtgI CCRYGG 1 cut(s) 271
BtsCI GGATG 1 cut(s) 232
Cfr13I GGNCC 1 cut(s) 275
Csp6I GTAC 1 cut(s) 311
CspCI CAANNNNNGTGG 2 cut(s) 132, 167
CviAII CATG 4 cut(s) 39, 76, 272, 381
CviJI RGCY 7 cut(s) 47, 59, 192, 262, 276, 387, 400
CviKI_1 RGCY 7 cut(s) 47, 59, 192, 262, 276, 387, 400
CviQI GTAC 1 cut(s) 311
Eco130I CCWWGG 3 cut(s) 222, 271, 401
EcoNI CCTNNNNNAGG 1 cut(s) 405
EcoRII CCWGG 3 cut(s) 42, 159, 277
EcoT14I CCWWGG 3 cut(s) 222, 271, 401
EcoT22I ATGCAT 1 cut(s) 321
ErhI CCWWGG 3 cut(s) 222, 271, 401
FaeI CATG 4 cut(s) 42, 79, 275, 384
FaiI YATR 8 cut(s) 40, 77, 95, 140, 189, 273, 382, 415
FaqI GGGAC 1 cut(s) 53
FatI CATG 4 cut(s) 38, 75, 271, 380
Fnu4HI GCNGC 2 cut(s) 48, 252
FokI GGATG 1 cut(s) 239
Fsp4HI GCNGC 2 cut(s) 48, 252
FspBI CTAG 1 cut(s) 233
GluI GCNGC 2 cut(s) 48, 252
HaeIII GGCC 3 cut(s) 47, 276, 400
Hin1II CATG 4 cut(s) 42, 79, 275, 384
HinfI GANTC 3 cut(s) 97, 115, 173
HphI GGTGA 2 cut(s) 176, 293
HpyAV CCTTC 1 cut(s) 301
HpyCH4V TGCA 2 cut(s) 251, 319
HpyF10VI GCNNNNNNNGC 1 cut(s) 56
Hsp92II CATG 4 cut(s) 42, 79, 275, 384
LmnI GCTCC 1 cut(s) 159
Lsp1109I GCAGC 1 cut(s) 263
LweI GCATC 2 cut(s) 217, 328
MaeI CTAG 1 cut(s) 233
MaeIII GTNAC 1 cut(s) 217
MboII GAAGA 1 cut(s) 98
MluCI AATT 4 cut(s) 51, 184, 246, 336
MmeI TCCRAC 1 cut(s) 181
MnlI CCTC 3 cut(s) 78, 137, 401
Mph1103I ATGCAT 1 cut(s) 321
MseI TTAA 1 cut(s) 264
MslI CAYNNNNRTG 1 cut(s) 162
MspA1I CMGCKG 1 cut(s) 59
MspCI CTTAAG 1 cut(s) 263
MspR9I CCNGG 3 cut(s) 44, 161, 279
MvaI CCWGG 3 cut(s) 44, 161, 279
MwoI GCNNNNNNNGC 1 cut(s) 56
NcoI CCATGG 1 cut(s) 271
NlaIII CATG 4 cut(s) 42, 79, 275, 384
NlaIV GGNNCC 1 cut(s) 89
NsiI ATGCAT 1 cut(s) 321
NspI RCATGY 1 cut(s) 42
OliI CACNNNNGTG 1 cut(s) 162
PfeI GAWTC 3 cut(s) 97, 115, 173
PkrI GCNGC 2 cut(s) 49, 253
PsiI TTATAA 1 cut(s) 415
Psp6I CCWGG 3 cut(s) 42, 159, 277
PspGI CCWGG 3 cut(s) 42, 159, 277
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 1 cut(s) 275
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 1 cut(s) 312
RsaNI GTAC 1 cut(s) 311
RseI CAYNNNNRTG 1 cut(s) 162
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 2 cut(s) 48, 252
Sau96I GGNCC 1 cut(s) 275
ScrFI CCNGG 3 cut(s) 44, 161, 279
SetI ASST 5 cut(s) 61, 93, 162, 194, 312
SfaNI GCATC 2 cut(s) 217, 328
SmiMI CAYNNNNRTG 1 cut(s) 162
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 4 cut(s) 51, 184, 246, 336
SsiI CCGC 1 cut(s) 48
SspMI CTAG 1 cut(s) 233
StyD4I CCNGG 3 cut(s) 42, 159, 277
StyI CCWWGG 3 cut(s) 222, 271, 401
TasI AATT 4 cut(s) 51, 184, 246, 336
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 3 cut(s) 97, 115, 173
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TseI GCWGC 1 cut(s) 251
TspDTI ATGAA 2 cut(s) 110, 348
Vha464I CTTAAG 1 cut(s) 263
XagI CCTNNNNNAGG 1 cut(s) 405
XceI RCATGY 1 cut(s) 42
XspI CTAG 1 cut(s) 233
Zsp2I ATGCAT 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.