RLG00000009014

Cytochrome b6-f complex iron-sulfur subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003668
Physical Location & Seq
Reverse (-)
45049360 .. 45050597
1238 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009014

Sequence Viewer

Length: 477 bp
ATGTTCTCTTTAGCACAGGCCCTGTCTATGATGCCCAGAAGGACCCAGATGATGGGAAAGGGTAAGGGAATGAGAATCACATGCCAGGCCGCAATTCCAGCTGATAATGTCCCTGACATGGGTAAGAGGAAGCTTATGAATCTGCTGCTTCTAACGAATCTCACTTCCCTCTGGTTTCCTATGCCACTTTCTTTGCTGCACCTGGGTGAGTTTGAATCATTGGTTCCTTTGTATTCCCTCTTTGATGTTGGAAGTGGTGGTCAAGTTGCCAAGGATGCTCTGCGAAACGATGCAATTGCAGCAGAATGGCTTAAGAACCATGGCCCCGGTGACAGGGCTTTCACACAAGGATTGAAGAACAAACCTCTTGCTGGAAATGTGACTGTCCTTGAGCTGCATTTGGACGCAAAGCTTTTATTGCCACTGGTGTGTGGTCCAGTTCCCCTTTGTCAACTGGACCATAACCTCCTTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
CytB6-F_Fe-S Rieske

Protein Analysis

159

Amino Acids

17.24

Weight (kDa)

8.88

Isoelectric Point (pI)

23.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TM_PetC PF25471 34 - 53 1.4e-06 Iron-sulfur subunit PetC transmembrane anchor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 52
AciI CCGC 1 cut(s) 90
AfiI CCNNNNNNNGG 5 cut(s) 52, 118, 119, 333, 371
AflII CTTAAG 1 cut(s) 311
AgsI TTSAA 2 cut(s) 215, 355
AjnI CCWGG 2 cut(s) 84, 201
AleI CACNNNNGTG 2 cut(s) 204, 427
AluBI AGCT 4 cut(s) 101, 133, 394, 412
AluI AGCT 4 cut(s) 101, 133, 394, 412
AlwNI CAGNNNCTG 1 cut(s) 22
AoxI GGCC 3 cut(s) 18, 87, 322
ApeKI GCWGC 4 cut(s) 145, 196, 299, 394
AspS9I GGNCC 5 cut(s) 19, 42, 323, 434, 457
AsuC2I CCSGG 1 cut(s) 327
AsuHPI GGTGA 2 cut(s) 218, 341
AvaII GGWCC 3 cut(s) 42, 434, 457
BbvI GCAGC 4 cut(s) 132, 183, 311, 381
BccI CCATC 1 cut(s) 46
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BciT130I CCWGG 2 cut(s) 86, 203
BcnI CCSGG 1 cut(s) 327
BfrI CTTAAG 1 cut(s) 311
BisI GCNGC 5 cut(s) 90, 146, 197, 300, 395
BlsI GCNGC 5 cut(s) 91, 147, 198, 301, 396
Bme1390I CCNGG 3 cut(s) 86, 203, 327
Bme18I GGWCC 3 cut(s) 42, 434, 457
BmgT120I GGNCC 5 cut(s) 19, 42, 323, 434, 457
BmiI GGNNCC 3 cut(s) 44, 225, 325
BmrFI CCNGG 3 cut(s) 86, 203, 327
BmsI GCATC 3 cut(s) 21, 265, 280
BpuEI CTTGAG 1 cut(s) 410
BpuMI CCSGG 1 cut(s) 327
BsaJI CCNNGG 4 cut(s) 202, 270, 319, 325
Bsc4I CCNNNNNNNGG 5 cut(s) 52, 118, 119, 333, 371
Bse1I ACTGG 3 cut(s) 429, 437, 459
BseBI CCWGG 2 cut(s) 86, 203
BseDI CCNNGG 4 cut(s) 202, 270, 319, 325
BseGI GGATG 1 cut(s) 280
BseLI CCNNNNNNNGG 5 cut(s) 52, 118, 119, 333, 371
BseNI ACTGG 3 cut(s) 429, 437, 459
BseXI GCAGC 4 cut(s) 132, 183, 311, 381
BsgI GTGCAG 1 cut(s) 182
BshFI GGCC 3 cut(s) 20, 89, 324
BsiSI CCGG 1 cut(s) 327
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 5 cut(s) 52, 118, 119, 333, 371
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 3 cut(s) 20, 89, 324
Bsp19I CCATGG 1 cut(s) 319
BspACI CCGC 1 cut(s) 90
BspANI GGCC 3 cut(s) 20, 89, 324
BspLI GGNNCC 3 cut(s) 44, 225, 325
BspTI CTTAAG 1 cut(s) 311
BsrI ACTGG 3 cut(s) 429, 437, 459
BssECI CCNNGG 4 cut(s) 202, 270, 319, 325
BssT1I CCWWGG 2 cut(s) 270, 319
Bst2UI CCWGG 2 cut(s) 86, 203
Bst4CI ACNGT 1 cut(s) 385
BstAFI CTTAAG 1 cut(s) 311
BstDSI CCRYGG 1 cut(s) 319
BstF5I GGATG 1 cut(s) 280
BstMWI GCNNNNNNNGC 4 cut(s) 98, 275, 299, 418
BstNI CCWGG 2 cut(s) 86, 203
BstNSI RCATGY 1 cut(s) 84
BstSCI CCNGG 3 cut(s) 84, 201, 325
BstV1I GCAGC 4 cut(s) 132, 183, 311, 381
BsuRI GGCC 3 cut(s) 20, 89, 324
BtgI CCRYGG 1 cut(s) 319
BtsCI GGATG 1 cut(s) 280
BtsIMutI CAGTG 1 cut(s) 422
CaiI CAGNNNCTG 1 cut(s) 22
Cfr13I GGNCC 5 cut(s) 19, 42, 323, 434, 457
CseI GACGC 1 cut(s) 413
CspCI CAANNNNNGTGG 2 cut(s) 174, 209
CviAII CATG 3 cut(s) 81, 118, 320
CviJI RGCY 9 cut(s) 20, 89, 101, 133, 310, 324, 338, 394, 412
CviKI_1 RGCY 9 cut(s) 20, 89, 101, 133, 310, 324, 338, 394, 412
Eco130I CCWWGG 2 cut(s) 270, 319
Eco47I GGWCC 3 cut(s) 42, 434, 457
EcoO109I RGGNCCY 2 cut(s) 19, 42
EcoRII CCWGG 2 cut(s) 84, 201
EcoT14I CCWWGG 2 cut(s) 270, 319
ErhI CCWWGG 2 cut(s) 270, 319
FaeI CATG 3 cut(s) 84, 121, 323
FaiI YATR 7 cut(s) 29, 82, 119, 137, 182, 321, 462
FaqI GGGAC 1 cut(s) 95
FatI CATG 3 cut(s) 80, 117, 319
Fnu4HI GCNGC 5 cut(s) 90, 146, 197, 300, 395
FokI GGATG 1 cut(s) 287
Fsp4HI GCNGC 5 cut(s) 90, 146, 197, 300, 395
GluI GCNGC 5 cut(s) 90, 146, 197, 300, 395
HaeIII GGCC 3 cut(s) 20, 89, 324
HapII CCGG 1 cut(s) 327
HgaI GACGC 1 cut(s) 413
Hin1II CATG 3 cut(s) 84, 121, 323
HincII GTYRAC 1 cut(s) 452
HindII GTYRAC 1 cut(s) 452
HindIII AAGCTT 2 cut(s) 131, 410
HinfI GANTC 4 cut(s) 75, 139, 157, 215
HpaII CCGG 1 cut(s) 327
HphI GGTGA 2 cut(s) 218, 341
Hpy166II GTNNAC 1 cut(s) 452
Hpy8I GTNNAC 1 cut(s) 452
HpyAV CCTTC 1 cut(s) 33
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4V TGCA 4 cut(s) 199, 293, 299, 397
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 275, 299, 418
Hsp92II CATG 3 cut(s) 84, 121, 323
Lsp1109I GCAGC 4 cut(s) 132, 183, 311, 381
LweI GCATC 3 cut(s) 21, 265, 280
MaeIII GTNAC 2 cut(s) 329, 379
MboII GAAGA 1 cut(s) 367
MfeI CAATTG 1 cut(s) 294
MluCI AATT 2 cut(s) 93, 294
MmeI TCCRAC 1 cut(s) 229
MnlI CCTC 5 cut(s) 120, 179, 248, 375, 476
MseI TTAA 1 cut(s) 312
MslI CAYNNNNRTG 2 cut(s) 204, 427
MspA1I CMGCKG 1 cut(s) 101
MspCI CTTAAG 1 cut(s) 311
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 3 cut(s) 86, 203, 327
MunI CAATTG 1 cut(s) 294
MvaI CCWGG 2 cut(s) 86, 203
MwoI GCNNNNNNNGC 4 cut(s) 98, 275, 299, 418
NciI CCSGG 1 cut(s) 327
NcoI CCATGG 1 cut(s) 319
NlaIII CATG 3 cut(s) 84, 121, 323
NlaIV GGNNCC 3 cut(s) 44, 225, 325
NmuCI GTSAC 2 cut(s) 329, 379
NspI RCATGY 1 cut(s) 84
OliI CACNNNNGTG 2 cut(s) 204, 427
PfeI GAWTC 4 cut(s) 75, 139, 157, 215
PflMI CCANNNNNTGG 1 cut(s) 52
PkrI GCNGC 5 cut(s) 91, 147, 198, 301, 396
PpuMI RGGWCCY 1 cut(s) 42
Psp5II RGGWCCY 1 cut(s) 42
Psp6I CCWGG 2 cut(s) 84, 201
PspGI CCWGG 2 cut(s) 84, 201
PspN4I GGNNCC 3 cut(s) 44, 225, 325
PspPI GGNCC 5 cut(s) 19, 42, 323, 434, 457
PspPPI RGGWCCY 1 cut(s) 42
PstNI CAGNNNCTG 1 cut(s) 22
PvuII CAGCTG 1 cut(s) 101
RseI CAYNNNNRTG 2 cut(s) 204, 427
SaqAI TTAA 1 cut(s) 312
SatI GCNGC 5 cut(s) 90, 146, 197, 300, 395
Sau96I GGNCC 5 cut(s) 19, 42, 323, 434, 457
ScrFI CCNGG 3 cut(s) 86, 203, 327
SetI ASST 7 cut(s) 103, 135, 204, 367, 396, 414, 468
SfaNI GCATC 3 cut(s) 21, 265, 280
SinI GGWCC 3 cut(s) 42, 434, 457
SmiMI CAYNNNNRTG 2 cut(s) 204, 427
SmlI CTYRAG 2 cut(s) 311, 389
SmoI CTYRAG 2 cut(s) 311, 389
Sse9I AATT 2 cut(s) 93, 294
SsiI CCGC 1 cut(s) 90
StyD4I CCNGG 3 cut(s) 84, 201, 325
StyI CCWWGG 2 cut(s) 270, 319
TaaI ACNGT 1 cut(s) 385
TasI AATT 2 cut(s) 93, 294
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 4 cut(s) 75, 139, 157, 215
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
TscAI CASTG 1 cut(s) 429
TseFI GTSAC 2 cut(s) 329, 379
TseI GCWGC 4 cut(s) 145, 196, 299, 394
Tsp45I GTSAC 2 cut(s) 329, 379
TspDTI ATGAA 1 cut(s) 152
TspRI CASTG 1 cut(s) 429
Van91I CCANNNNNTGG 1 cut(s) 52
Vha464I CTTAAG 1 cut(s) 311
VpaK11BI GGWCC 3 cut(s) 42, 434, 457
XceI RCATGY 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.