Rh2DG577500

Cytochrome b6-f complex iron-sulfur subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003668
Physical Location & Seq
Forward (+)
80833941 .. 80836562
2622 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG577500.1

Sequence Viewer

Length: 561 bp
ATGCCCCCTTATCCACTAGATGTCGTGGTAGTCATTGCCAATCATCAAATATACACCCATAACACAAAAAAAACACCATCATTTCCAAGAATTCCAGATACAATGGCTGCCTCTACTCTATCTTCTGCTACCACTTCACAGCTATGCTCCAGCAAGAGTGCTATGTTCTCTTCAGCACAGGCCCTGTCTGTGATGCCCAGGAGGACCCAGATGATGGGAAAGGGAAAGGGAATGAGAATCACATGCCAGGCAGCAATTCCAGCTGATAATGTCCCTGACATGGGTAAGAGGAAGCTTCTGAATTTGCTTCTTCTGGGTGCTCTCTCACTTCCCTCTGGTTTCATGCTGGTTCCCTATGCTACTTTCTTTGCTCCACCTGGCTCTGGTGCTGGAAGTAGTGGTCAAGTTGCCAAGGATGCTATCGGAAACGATGTAATTGCATCAGAATGGCTTAAGACCCATGGCCCTGGTGACAGGACTCTCACACAAGGATTGAAGGTACTGATACATCTTACTACTATTTCAATTGGAAAGTTCAAGTCTCATTTTGCAATTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
CytB6-F_Fe-S Rieske

Protein Analysis

186

Amino Acids

19.74

Weight (kDa)

9.96

Isoelectric Point (pI)

26.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TM_PetC PF25471 88 - 134 4e-21 Iron-sulfur subunit PetC transmembrane anchor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 214
AcsI RAATTY 2 cut(s) 90, 301
AcuI CTGAAG 1 cut(s) 156
AfaI GTAC 1 cut(s) 501
AfiI CCNNNNNNNGG 4 cut(s) 214, 280, 281, 383
AflII CTTAAG 1 cut(s) 452
AgsI TTSAA 4 cut(s) 496, 525, 538, 557
AjnI CCWGG 4 cut(s) 197, 246, 376, 466
AluBI AGCT 3 cut(s) 142, 263, 295
AluI AGCT 3 cut(s) 142, 263, 295
Alw21I GWGCWC 1 cut(s) 322
Alw26I GTCTC 1 cut(s) 546
AlwNI CAGNNNCTG 1 cut(s) 184
AoxI GGCC 2 cut(s) 180, 463
ApeKI GCWGC 2 cut(s) 107, 251
ApoI RAATTY 2 cut(s) 90, 301
AspS9I GGNCC 3 cut(s) 181, 204, 464
AsuHPI GGTGA 1 cut(s) 482
AvaII GGWCC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 322
BbvI GCAGC 2 cut(s) 94, 263
BccI CCATC 2 cut(s) 85, 208
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 4 cut(s) 199, 248, 378, 468
BcoDI GTCTC 1 cut(s) 546
BfaI CTAG 1 cut(s) 17
BfrI CTTAAG 1 cut(s) 452
BisI GCNGC 2 cut(s) 108, 252
BlsI GCNGC 2 cut(s) 109, 253
Bme1390I CCNGG 4 cut(s) 199, 248, 378, 468
Bme18I GGWCC 1 cut(s) 204
BmgT120I GGNCC 3 cut(s) 181, 204, 464
BmiI GGNNCC 2 cut(s) 206, 351
BmrFI CCNGG 4 cut(s) 199, 248, 378, 468
BmsI GCATC 3 cut(s) 183, 406, 449
BpmI CTGGAG 1 cut(s) 133
BsaJI CCNNGG 4 cut(s) 197, 411, 460, 466
Bsc4I CCNNNNNNNGG 4 cut(s) 214, 280, 281, 383
Bse3DI GCAATG 1 cut(s) 33
BseBI CCWGG 4 cut(s) 199, 248, 378, 468
BseDI CCNNGG 4 cut(s) 197, 411, 460, 466
BseGI GGATG 1 cut(s) 421
BseLI CCNNNNNNNGG 4 cut(s) 214, 280, 281, 383
BseMI GCAATG 1 cut(s) 33
BseXI GCAGC 2 cut(s) 94, 263
BshFI GGCC 2 cut(s) 182, 465
BsiHKAI GWGCWC 1 cut(s) 322
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 4 cut(s) 214, 280, 281, 383
BsmAI GTCTC 1 cut(s) 546
BsmFI GGGAC 1 cut(s) 257
BsnI GGCC 2 cut(s) 182, 465
Bsp1286I GDGCHC 1 cut(s) 322
Bsp19I CCATGG 1 cut(s) 460
BspANI GGCC 2 cut(s) 182, 465
BspLI GGNNCC 2 cut(s) 206, 351
BspTI CTTAAG 1 cut(s) 452
BsrDI GCAATG 1 cut(s) 33
BssECI CCNNGG 4 cut(s) 197, 411, 460, 466
BssT1I CCWWGG 2 cut(s) 411, 460
Bst2UI CCWGG 4 cut(s) 199, 248, 378, 468
Bst6I CTCTTC 1 cut(s) 175
BstAFI CTTAAG 1 cut(s) 452
BstDSI CCRYGG 1 cut(s) 460
BstF5I GGATG 1 cut(s) 421
BstMAI GTCTC 1 cut(s) 546
BstMWI GCNNNNNNNGC 2 cut(s) 260, 416
BstNI CCWGG 4 cut(s) 199, 248, 378, 468
BstNSI RCATGY 1 cut(s) 246
BstSCI CCNGG 4 cut(s) 197, 246, 376, 466
BstV1I GCAGC 2 cut(s) 94, 263
BstXI CCANNNNNNTGG 1 cut(s) 467
BsuRI GGCC 2 cut(s) 182, 465
BtgI CCRYGG 1 cut(s) 460
BtsCI GGATG 1 cut(s) 421
CaiI CAGNNNCTG 1 cut(s) 184
Cfr13I GGNCC 3 cut(s) 181, 204, 464
Csp6I GTAC 1 cut(s) 500
CviAII CATG 4 cut(s) 243, 280, 343, 461
CviJI RGCY 8 cut(s) 107, 142, 182, 263, 295, 381, 451, 465
CviKI_1 RGCY 8 cut(s) 107, 142, 182, 263, 295, 381, 451, 465
CviQI GTAC 1 cut(s) 500
Eam1104I CTCTTC 1 cut(s) 175
EarI CTCTTC 1 cut(s) 175
Eco130I CCWWGG 2 cut(s) 411, 460
Eco47I GGWCC 1 cut(s) 204
Eco57I CTGAAG 1 cut(s) 156
EcoO109I RGGNCCY 2 cut(s) 181, 204
EcoRI GAATTC 1 cut(s) 90
EcoRII CCWGG 4 cut(s) 197, 246, 376, 466
EcoT14I CCWWGG 2 cut(s) 411, 460
ErhI CCWWGG 2 cut(s) 411, 460
FaeI CATG 4 cut(s) 246, 283, 346, 464
FaiI YATR 9 cut(s) 52, 60, 145, 164, 244, 281, 344, 357, 462
FaqI GGGAC 1 cut(s) 257
FatI CATG 4 cut(s) 242, 279, 342, 460
Fnu4HI GCNGC 2 cut(s) 108, 252
FokI GGATG 1 cut(s) 428
Fsp4HI GCNGC 2 cut(s) 108, 252
FspBI CTAG 1 cut(s) 17
GluI GCNGC 2 cut(s) 108, 252
GsuI CTGGAG 1 cut(s) 133
HaeIII GGCC 2 cut(s) 182, 465
Hin1II CATG 4 cut(s) 246, 283, 346, 464
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 2 cut(s) 237, 478
HphI GGTGA 1 cut(s) 482
Hpy188I TCNGA 3 cut(s) 300, 425, 445
Hpy188III TCNNGA 1 cut(s) 95
HpyAV CCTTC 1 cut(s) 490
HpyCH4V TGCA 2 cut(s) 440, 551
HpyF10VI GCNNNNNNNGC 2 cut(s) 260, 416
Hsp92II CATG 4 cut(s) 246, 283, 346, 464
LmnI GCTCC 2 cut(s) 152, 376
Lsp1109I GCAGC 2 cut(s) 94, 263
LweI GCATC 3 cut(s) 183, 406, 449
MaeI CTAG 1 cut(s) 17
MaeIII GTNAC 1 cut(s) 470
MboII GAAGA 3 cut(s) 114, 162, 302
MfeI CAATTG 1 cut(s) 525
MhlI GDGCHC 1 cut(s) 322
MluCI AATT 6 cut(s) 90, 255, 301, 435, 525, 552
MlyI GAGTC 1 cut(s) 472
MnlI CCTC 4 cut(s) 121, 195, 282, 343
MseI TTAA 1 cut(s) 453
MslI CAYNNNNRTG 2 cut(s) 142, 445
MspA1I CMGCKG 1 cut(s) 263
MspCI CTTAAG 1 cut(s) 452
MspR9I CCNGG 4 cut(s) 199, 248, 378, 468
MunI CAATTG 1 cut(s) 525
MvaI CCWGG 4 cut(s) 199, 248, 378, 468
MwoI GCNNNNNNNGC 2 cut(s) 260, 416
NcoI CCATGG 1 cut(s) 460
NlaIII CATG 4 cut(s) 246, 283, 346, 464
NlaIV GGNNCC 2 cut(s) 206, 351
NmuCI GTSAC 1 cut(s) 470
NspI RCATGY 1 cut(s) 246
PfeI GAWTC 1 cut(s) 237
PflMI CCANNNNNTGG 1 cut(s) 214
PkrI GCNGC 2 cut(s) 109, 253
PleI GAGTC 1 cut(s) 472
PpsI GAGTC 1 cut(s) 472
PpuMI RGGWCCY 1 cut(s) 204
Psp5II RGGWCCY 1 cut(s) 204
Psp6I CCWGG 4 cut(s) 197, 246, 376, 466
PspGI CCWGG 4 cut(s) 197, 246, 376, 466
PspN4I GGNNCC 2 cut(s) 206, 351
PspPI GGNCC 3 cut(s) 181, 204, 464
PspPPI RGGWCCY 1 cut(s) 204
PstNI CAGNNNCTG 1 cut(s) 184
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 1 cut(s) 501
RsaNI GTAC 1 cut(s) 500
RseI CAYNNNNRTG 2 cut(s) 142, 445
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 2 cut(s) 108, 252
Sau96I GGNCC 3 cut(s) 181, 204, 464
SchI GAGTC 1 cut(s) 472
ScrFI CCNGG 4 cut(s) 199, 248, 378, 468
SduI GDGCHC 1 cut(s) 322
SetI ASST 5 cut(s) 144, 265, 297, 379, 501
SfaNI GCATC 3 cut(s) 183, 406, 449
SinI GGWCC 1 cut(s) 204
SmiMI CAYNNNNRTG 2 cut(s) 142, 445
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
Sse9I AATT 6 cut(s) 90, 255, 301, 435, 525, 552
SspMI CTAG 1 cut(s) 17
StyD4I CCNGG 4 cut(s) 197, 246, 376, 466
StyI CCWWGG 2 cut(s) 411, 460
TasI AATT 6 cut(s) 90, 255, 301, 435, 525, 552
TfiI GAWTC 1 cut(s) 237
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TseFI GTSAC 1 cut(s) 470
TseI GCWGC 2 cut(s) 107, 251
Tsp45I GTSAC 1 cut(s) 470
TspDTI ATGAA 1 cut(s) 331
Van91I CCANNNNNTGG 1 cut(s) 214
Vha464I CTTAAG 1 cut(s) 452
VpaK11BI GGWCC 1 cut(s) 204
XapI RAATTY 2 cut(s) 90, 301
XceI RCATGY 1 cut(s) 246
XspI CTAG 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.