Rh6DG102900

NEDD8-conjugating enzyme Ubc12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003668
Physical Location & Seq
Forward (+)
12969310 .. 12988902
19593 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG102900.1

Sequence Viewer

Length: 1050 bp
ATGTTCTCTTCAGCACAGGCTCTGTCTCTGATGCCCAGGAGGACCCAGATGATGGGAAAGGGAAAGGGAATGAGAATCACATGCCAAGCTGCAATTCCAGCTGATAATATCCCTGACATGGGTAAGAGGAAGCTTATGAATCTGCTTCTTCTAACGAATCTCACTTCCCTCTGGTTTCCTATGCCACTTTGTTTGCTCCACCTGGGTCTTTGCTATGGGAAGTTGGAAGCTGGACTACTTACATTCATTATACCCATTGTTCTTCTTGGGCACCTGGGAGCACTTAGAGTTTCCATGACAAGTCCAGGATTTATCTATGAGCCTTATGCACCTAGTGAAAATGTTCCATTTTTCCGGAGATTCCTTTTGAGCTCGAGCTCTCTCTCTTCTTCTCCTCCTCCCTTTGGCTTTGGCTTTGCTCTCTTTGCTGTTCTAAGCAGGGAAGTCTTACATCTGTTTTTGCGGGGGGACTCCCGACAGTTCAACTCAAGCGGAAAAATGATTAAGCTATTTAAAGTGAAGGAAAAGCAGAGGGAGCTTGCTGAAAATGCCAATGGAAAGACACTGGTTAAGAAGCAAAGCGCAGGAGAATTACGGCTTCATAGAGATATTAGTGAGCTGAACCTACAGAAAACATGTACCATAGCATTCCCCAATGGTAAGGACGATTTAATGAACTTTGAGGTTACCGTCCGGCCTGATGAAGGATACTATCTAGGCGGTACATTTATGTTCACATTTCAAGTTTCTCCCATGTATCCTCATGAGGCGCCAAAGGTCAAGTGCAAGACAAAGGTCTATCATCCTAATATTGACTTGGAAGGAAACGTCTGCCTCAACATTCTAAGAGAAGACTGGAAACCTGTTTTGAATATCAATACTATAATATATGGACTCTTTCATCTTTTTACGCAACCCAATTATGAAGATCCCCTTAATCATGATGCAGCTGCTGTCCTAAGGGATAACCCCAAGATGTTTGAGTCCAATGTGAGAAGAGCTATGTCTGGTGGGTATGTGGGGCAGACCTTATTCCCGCGGTGCATGTAG

Protein Analysis

349

Amino Acids

39.31

Weight (kDa)

9.38

Isoelectric Point (pI)

48.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 199 - 334 9.3e-40 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 270, 769
AccB7I CCANNNNNTGG 1 cut(s) 52
AccII CGCG 1 cut(s) 1039
AccIII TCCGGA 1 cut(s) 354
AciI CCGC 5 cut(s) 463, 492, 720, 1037, 1039
AclWI GGATC 1 cut(s) 923
AcyI GRCGYC 1 cut(s) 770
AdeI CACNNNGTG 1 cut(s) 335
AfaI GTAC 2 cut(s) 640, 724
AfiI CCNNNNNNNGG 5 cut(s) 52, 118, 119, 404, 704
AflIII ACRYGT 1 cut(s) 635
AgsI TTSAA 3 cut(s) 484, 743, 871
AjnI CCWGG 4 cut(s) 35, 201, 273, 304
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
Alw21I GWGCWC 3 cut(s) 283, 374, 380
Alw26I GTCTC 1 cut(s) 30
AlwI GGATC 1 cut(s) 923
AlwNI CAGNNNCTG 2 cut(s) 22, 953
Ama87I CYCGRG 1 cut(s) 373
Aor13HI TCCGGA 1 cut(s) 354
AoxI GGCC 1 cut(s) 695
ApeKI GCWGC 3 cut(s) 89, 947, 950
Asp700I GAANNNNTTC 1 cut(s) 342
AspLEI GCGC 2 cut(s) 584, 772
AspS9I GGNCC 1 cut(s) 42
AvaI CYCGRG 1 cut(s) 373
AvaII GGWCC 1 cut(s) 42
AxyI CCTNAGG 1 cut(s) 959
BaeGI GKGCMC 1 cut(s) 273
BanI GGYRCC 2 cut(s) 270, 769
BanII GRGCYC 2 cut(s) 374, 380
BarI GAAGNNNNNNTAC 2 cut(s) 219, 251
BbsI GAAGAC 1 cut(s) 858
Bbv12I GWGCWC 3 cut(s) 283, 374, 380
BbvI GCAGC 3 cut(s) 76, 937, 959
BccI CCATC 1 cut(s) 46
BceAI ACGGC 1 cut(s) 611
BciT130I CCWGG 4 cut(s) 37, 203, 275, 306
BciVI GTATCC 2 cut(s) 701, 768
BcoDI GTCTC 1 cut(s) 30
BfaI CTAG 2 cut(s) 333, 716
BfmI CTRYAG 1 cut(s) 626
BfoI RGCGCY 1 cut(s) 773
BfuI GTATCC 2 cut(s) 701, 768
BisI GCNGC 3 cut(s) 90, 948, 951
BlsI GCNGC 3 cut(s) 91, 949, 952
Bme1390I CCNGG 4 cut(s) 37, 203, 275, 306
Bme18I GGWCC 1 cut(s) 42
BmeT110I CYCGRG 1 cut(s) 373
BmgT120I GGNCC 1 cut(s) 42
BmiI GGNNCC 3 cut(s) 44, 272, 771
BmrFI CCNGG 4 cut(s) 37, 203, 275, 306
BmsI GCATC 2 cut(s) 21, 934
BoxI GACNNNNGTC 1 cut(s) 794
BpiI GAAGAC 1 cut(s) 858
BpuEI CTTGAG 1 cut(s) 472
BsaHI GRCGYC 1 cut(s) 770
BsaJI CCNNGG 4 cut(s) 35, 202, 274, 1037
BsaWI WCCGGW 1 cut(s) 354
Bsc4I CCNNNNNNNGG 5 cut(s) 52, 118, 119, 404, 704
Bse1I ACTGG 2 cut(s) 570, 860
Bse21I CCTNAGG 1 cut(s) 959
BseAI TCCGGA 1 cut(s) 354
BseBI CCWGG 4 cut(s) 37, 203, 275, 306
BseDI CCNNGG 4 cut(s) 35, 202, 274, 1037
BseGI GGATG 1 cut(s) 802
BseLI CCNNNNNNNGG 5 cut(s) 52, 118, 119, 404, 704
BseNI ACTGG 2 cut(s) 570, 860
BseRI GAGGAG 2 cut(s) 384, 387
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 3 cut(s) 76, 937, 959
Bsh1236I CGCG 1 cut(s) 1039
BshFI GGCC 1 cut(s) 697
BshNI GGYRCC 2 cut(s) 270, 769
BsiHKAI GWGCWC 3 cut(s) 283, 374, 380
BsiHKCI CYCGRG 1 cut(s) 373
BsiSI CCGG 2 cut(s) 355, 694
BslFI GGGAC 1 cut(s) 482
BslI CCNNNNNNNGG 5 cut(s) 52, 118, 119, 404, 704
BsmAI GTCTC 1 cut(s) 30
BsmFI GGGAC 1 cut(s) 482
BsmI GAATGC 1 cut(s) 647
BsnI GGCC 1 cut(s) 697
BsoBI CYCGRG 1 cut(s) 373
Bsp1286I GDGCHC 4 cut(s) 273, 283, 374, 380
Bsp13I TCCGGA 1 cut(s) 354
Bsp143I GATC 1 cut(s) 928
BspACI CCGC 5 cut(s) 463, 492, 720, 1037, 1039
BspANI GGCC 1 cut(s) 697
BspEI TCCGGA 1 cut(s) 354
BspFNI CGCG 1 cut(s) 1039
BspHI TCATGA 2 cut(s) 763, 940
BspLI GGNNCC 3 cut(s) 44, 272, 771
BspPI GGATC 1 cut(s) 923
BspQI GCTCTTC 1 cut(s) 991
BspT107I GGYRCC 2 cut(s) 270, 769
BsrI ACTGG 2 cut(s) 570, 860
BssECI CCNNGG 4 cut(s) 35, 202, 274, 1037
BssMI GATC 1 cut(s) 928
BssNI GRCGYC 1 cut(s) 770
Bst2UI CCWGG 4 cut(s) 37, 203, 275, 306
Bst4CI ACNGT 2 cut(s) 480, 691
Bst6I CTCTTC 3 cut(s) 13, 391, 991
BstACI GRCGYC 1 cut(s) 770
BstC8I GCNNGC 1 cut(s) 540
BstDEI CTNAG 4 cut(s) 284, 434, 845, 959
BstDSI CCRYGG 1 cut(s) 1037
BstEII GGTNACC 1 cut(s) 685
BstENI CCTNNNNNAGG 1 cut(s) 702
BstF5I GGATG 1 cut(s) 802
BstFNI CGCG 1 cut(s) 1039
BstH2I RGCGCY 1 cut(s) 773
BstHHI GCGC 2 cut(s) 584, 772
BstKTI GATC 1 cut(s) 931
BstMAI GTCTC 1 cut(s) 30
BstMBI GATC 1 cut(s) 928
BstMWI GCNNNNNNNGC 4 cut(s) 98, 425, 535, 548
BstNI CCWGG 4 cut(s) 37, 203, 275, 306
BstNSI RCATGY 3 cut(s) 84, 639, 1048
BstPAI GACNNNNGTC 1 cut(s) 794
BstPI GGTNACC 1 cut(s) 685
BstSCI CCNGG 4 cut(s) 35, 201, 273, 304
BstSFI CTRYAG 1 cut(s) 626
BstSLI GKGCMC 1 cut(s) 273
BstUI CGCG 1 cut(s) 1039
BstV1I GCAGC 3 cut(s) 76, 937, 959
BstV2I GAAGAC 1 cut(s) 858
BstX2I RGATCY 1 cut(s) 928
BstYI RGATCY 1 cut(s) 928
Bsu36I CCTNAGG 1 cut(s) 959
BsuI GTATCC 2 cut(s) 701, 768
BsuRI GGCC 1 cut(s) 697
BtgI CCRYGG 1 cut(s) 1037
BtsCI GGATG 1 cut(s) 802
BtsIMutI CAGTG 1 cut(s) 563
Cac8I GCNNGC 1 cut(s) 540
CaiI CAGNNNCTG 2 cut(s) 22, 953
CciI TCATGA 2 cut(s) 763, 940
CfoI GCGC 2 cut(s) 584, 772
Cfr13I GGNCC 1 cut(s) 42
Cfr42I CCGCGG 1 cut(s) 1040
Csp6I GTAC 2 cut(s) 639, 723
CspCI CAANNNNNGTGG 2 cut(s) 174, 209
CviAII CATG 8 cut(s) 81, 118, 295, 636, 754, 764, 941, 1045
CviQI GTAC 2 cut(s) 639, 723
DdeI CTNAG 4 cut(s) 284, 434, 845, 959
DinI GGCGCC 1 cut(s) 771
DpnI GATC 1 cut(s) 930
DpnII GATC 1 cut(s) 928
DraI TTTAAA 1 cut(s) 514
DraIII CACNNNGTG 1 cut(s) 335
Eam1104I CTCTTC 3 cut(s) 13, 391, 991
EarI CTCTTC 3 cut(s) 13, 391, 991
Ecl136II GAGCTC 2 cut(s) 372, 378
Eco24I GRGCYC 2 cut(s) 374, 380
Eco47I GGWCC 1 cut(s) 42
Eco53kI GAGCTC 2 cut(s) 372, 378
Eco81I CCTNAGG 1 cut(s) 959
Eco88I CYCGRG 1 cut(s) 373
Eco91I GGTNACC 1 cut(s) 685
EcoICRI GAGCTC 2 cut(s) 372, 378
EcoNI CCTNNNNNAGG 1 cut(s) 702
EcoO109I RGGNCCY 1 cut(s) 42
EcoO65I GGTNACC 1 cut(s) 685
EcoRII CCWGG 4 cut(s) 35, 201, 273, 304
EcoT38I GRGCYC 2 cut(s) 374, 380
EgeI GGCGCC 1 cut(s) 771
EheI GGCGCC 1 cut(s) 771
FaeI CATG 8 cut(s) 84, 121, 298, 639, 757, 767, 944, 1048
FalI AAGNNNNNCTT 2 cut(s) 918, 950
FaqI GGGAC 1 cut(s) 482
FatI CATG 8 cut(s) 80, 117, 294, 635, 753, 763, 940, 1044
FauI CCCGC 2 cut(s) 456, 1044
Fnu4HI GCNGC 3 cut(s) 90, 948, 951
FokI GGATG 1 cut(s) 789
FriOI GRGCYC 2 cut(s) 374, 380
Fsp4HI GCNGC 3 cut(s) 90, 948, 951
FspBI CTAG 2 cut(s) 333, 716
GlaI GCGC 2 cut(s) 583, 771
GluI GCNGC 3 cut(s) 90, 948, 951
HaeII RGCGCY 1 cut(s) 773
HaeIII GGCC 1 cut(s) 697
HapII CCGG 2 cut(s) 355, 694
HhaI GCGC 2 cut(s) 584, 772
Hin1I GRCGYC 1 cut(s) 770
Hin1II CATG 8 cut(s) 84, 121, 298, 639, 757, 767, 944, 1048
Hin6I GCGC 2 cut(s) 582, 770
HinP1I GCGC 2 cut(s) 582, 770
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 7 cut(s) 75, 139, 157, 360, 470, 894, 983
HpaII CCGG 2 cut(s) 355, 694
Hpy166II GTNNAC 1 cut(s) 735
Hpy188I TCNGA 1 cut(s) 30
Hpy188III TCNNGA 4 cut(s) 355, 474, 764, 941
Hpy8I GTNNAC 1 cut(s) 735
HpyAV CCTTC 3 cut(s) 514, 698, 815
HpyCH4III ACNGT 2 cut(s) 480, 691
HpyCH4IV ACGT 1 cut(s) 828
HpyCH4V TGCA 5 cut(s) 92, 329, 786, 947, 1044
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 425, 535, 548
HpyF3I CTNAG 4 cut(s) 284, 434, 845, 959
HpySE526I ACGT 1 cut(s) 828
Hsp92I GRCGYC 1 cut(s) 770
Hsp92II CATG 8 cut(s) 84, 121, 298, 639, 757, 767, 944, 1048
HspAI GCGC 2 cut(s) 582, 770
KasI GGCGCC 1 cut(s) 769
Kpn2I TCCGGA 1 cut(s) 354
KspI CCGCGG 1 cut(s) 1040
Kzo9I GATC 1 cut(s) 928
LguI GCTCTTC 1 cut(s) 991
LmnI GCTCC 3 cut(s) 201, 278, 535
Lsp1109I GCAGC 3 cut(s) 76, 937, 959
LweI GCATC 2 cut(s) 21, 934
MaeI CTAG 2 cut(s) 333, 716
MaeII ACGT 1 cut(s) 828
MaeIII GTNAC 1 cut(s) 685
MalI GATC 1 cut(s) 930
MboI GATC 1 cut(s) 928
MboII GAAGA 7 cut(s) 140, 254, 378, 381, 863, 938, 1008
MflI RGATCY 1 cut(s) 928
MhlI GDGCHC 4 cut(s) 273, 283, 374, 380
MluCI AATT 3 cut(s) 93, 590, 919
Mly113I GGCGCC 1 cut(s) 770
MlyI GAGTC 3 cut(s) 464, 888, 992
MmeI TCCRAC 1 cut(s) 204
MroI TCCGGA 1 cut(s) 354
MroXI GAANNNNTTC 1 cut(s) 342
MseI TTAA 5 cut(s) 504, 513, 570, 671, 936
MspA1I CMGCKG 3 cut(s) 101, 950, 1039
MspI CCGG 2 cut(s) 355, 694
MspR9I CCNGG 4 cut(s) 37, 203, 275, 306
Mva1269I GAATGC 1 cut(s) 647
MvaI CCWGG 4 cut(s) 37, 203, 275, 306
MvnI CGCG 1 cut(s) 1039
MwoI GCNNNNNNNGC 4 cut(s) 98, 425, 535, 548
NarI GGCGCC 1 cut(s) 770
NdeII GATC 1 cut(s) 928
NlaIII CATG 8 cut(s) 84, 121, 298, 639, 757, 767, 944, 1048
NlaIV GGNNCC 3 cut(s) 44, 272, 771
NspI RCATGY 3 cut(s) 84, 639, 1048
PaeR7I CTCGAG 1 cut(s) 373
PagI TCATGA 2 cut(s) 763, 940
PciI ACATGT 1 cut(s) 635
PciSI GCTCTTC 1 cut(s) 991
PctI GAATGC 1 cut(s) 647
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 4 cut(s) 75, 139, 157, 360
PflMI CCANNNNNTGG 1 cut(s) 52
PfoI TCCNGGA 1 cut(s) 304
PkrI GCNGC 3 cut(s) 91, 949, 952
PleI GAGTC 3 cut(s) 464, 888, 991
PluTI GGCGCC 1 cut(s) 773
PpsI GAGTC 3 cut(s) 464, 888, 991
PpuMI RGGWCCY 1 cut(s) 42
PscI ACATGT 1 cut(s) 635
PshAI GACNNNNGTC 1 cut(s) 794
Psp124BI GAGCTC 2 cut(s) 374, 380
Psp5II RGGWCCY 1 cut(s) 42
Psp6I CCWGG 4 cut(s) 35, 201, 273, 304
PspEI GGTNACC 1 cut(s) 685
PspGI CCWGG 4 cut(s) 35, 201, 273, 304
PspN4I GGNNCC 3 cut(s) 44, 272, 771
PspPI GGNCC 1 cut(s) 42
PspPPI RGGWCCY 1 cut(s) 42
PspXI VCTCGAGB 1 cut(s) 373
PstNI CAGNNNCTG 2 cut(s) 22, 953
PsuI RGATCY 1 cut(s) 928
PvuII CAGCTG 2 cut(s) 101, 950
RsaI GTAC 2 cut(s) 640, 724
RsaNI GTAC 2 cut(s) 639, 723
SacI GAGCTC 2 cut(s) 374, 380
SacII CCGCGG 1 cut(s) 1040
SapI GCTCTTC 1 cut(s) 991
SaqAI TTAA 5 cut(s) 504, 513, 570, 671, 936
SatI GCNGC 3 cut(s) 90, 948, 951
Sau3AI GATC 1 cut(s) 928
Sau96I GGNCC 1 cut(s) 42
SchI GAGTC 3 cut(s) 464, 888, 992
ScrFI CCNGG 4 cut(s) 37, 203, 275, 306
SduI GDGCHC 4 cut(s) 273, 283, 374, 380
SfaNI GCATC 2 cut(s) 21, 934
SfcI CTRYAG 1 cut(s) 626
SfoI GGCGCC 1 cut(s) 771
Sfr274I CTCGAG 1 cut(s) 373
Sfr303I CCGCGG 1 cut(s) 1040
SgrBI CCGCGG 1 cut(s) 1040
SinI GGWCC 1 cut(s) 42
SlaI CTCGAG 1 cut(s) 373
SmlI CTYRAG 2 cut(s) 373, 487
SmoI CTYRAG 2 cut(s) 373, 487
Sse9I AATT 3 cut(s) 93, 590, 919
SsiI CCGC 5 cut(s) 463, 492, 720, 1037, 1039
SspDI GGCGCC 1 cut(s) 769
SspI AATATT 1 cut(s) 811
SspMI CTAG 2 cut(s) 333, 716
SstI GAGCTC 2 cut(s) 374, 380
StyD4I CCNGG 4 cut(s) 35, 201, 273, 304
TaaI ACNGT 2 cut(s) 480, 691
TaiI ACGT 1 cut(s) 831
TaqI TCGA 1 cut(s) 374
TasI AATT 3 cut(s) 93, 590, 919
TfiI GAWTC 4 cut(s) 75, 139, 157, 360
Tru1I TTAA 5 cut(s) 504, 513, 570, 671, 936
Tru9I TTAA 5 cut(s) 504, 513, 570, 671, 936
TscAI CASTG 1 cut(s) 570
TseI GCWGC 3 cut(s) 89, 947, 950
TspDTI ATGAA 7 cut(s) 152, 235, 590, 689, 717, 890, 939
TspRI CASTG 1 cut(s) 570
Van91I CCANNNNNTGG 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 42
XagI CCTNNNNNAGG 1 cut(s) 702
XceI RCATGY 3 cut(s) 84, 639, 1048
XhoI CTCGAG 1 cut(s) 373
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 2 cut(s) 333, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.