Rorug02G0488200

Cytochrome b6-f complex iron-sulfur subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003668
Physical Location & Seq
Reverse (-)
61814458 .. 61819445
4988 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0488200.1

Sequence Viewer

Length: 753 bp
ATGGAGTCCCTAATACTAATCCTTTACATCCTCATTTCCACCACAGCTTCAAGCACTCTATGTGATGCAAGAAGTTTATCCCAAACCCAGAAAGCAGAACAACACAGAAGTACAACTCAAATACTGAAGAAGGTGAAAGTAACAAACCACATAGACATACCAGTAGTACAAGCCAGCTCTCCATTTTCTTCTCCACCTTCTGATTCACCAGCACCAAACCCATTGCCTACTGCAGCTGTCCCTCCTAATCTTCCCTATTGTGTAAATCCACAACCACCTGCCAGTATTTTATCTCCCACATACCCACCTCAAAATGGGCCTAGCCCACACCAACCCTGCATCATTCTAAGCCCACCCAACATTCCTGTAAGCCCACCCATAAGTCCTCTAACCCCACCAGCAGTCAATCCCTCCCCTACAGTCAACTCCCCACCACCAACTGGACCAAACAACACTCCTCCACACACAAAGCCCCAGTCTGGAGTGTGGTGTGTAGCCAAGCCGACCGTGCCCGACCCGATAGTCCAAGCGGCCATGGACTACGCGTGCGGGTCGGGGGCAGACTGCAAGTCAATCCAGCGAAACGGATCGTGCTACCAACCCGACACGTTGCTCTCCCATGCATCGTATGCGTTCAACAATTACTGGCAGAGAAGGAAGGTGGCCGGAGGGACTTGTGATTTTGGAGGGACAGCGATGCTGGTCACTGCTGATCCAAGTTTTGATGGATGCCAGTTCTTCTACAATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
CytB6-F_Fe-S Rieske

Protein Analysis

250

Amino Acids

26.43

Weight (kDa)

7.54

Isoelectric Point (pI)

69.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 163 - 233 5.2e-23 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 286
AasI GACNNNNNNGTC 1 cut(s) 521
Acc36I ACCTGC 1 cut(s) 286
AccB7I CCANNNNNTGG 1 cut(s) 440
AccII CGCG 1 cut(s) 545
AciI CCGC 2 cut(s) 530, 549
AclWI GGATC 2 cut(s) 595, 707
AcoI YGGCCR 2 cut(s) 531, 663
AcuI CTGAAG 1 cut(s) 146
AfaI GTAC 2 cut(s) 112, 168
AfiI CCNNNNNNNGG 3 cut(s) 314, 440, 479
AflIII ACRYGT 2 cut(s) 543, 606
AgsI TTSAA 2 cut(s) 51, 637
AhdI GACNNNNNGTC 1 cut(s) 568
AluBI AGCT 3 cut(s) 47, 177, 236
AluI AGCT 3 cut(s) 47, 177, 236
AlwI GGATC 2 cut(s) 595, 707
AoxI GGCC 3 cut(s) 317, 531, 663
ApeKI GCWGC 1 cut(s) 233
ArsI GACNNNNNNTTYG 2 cut(s) 461, 493
AspS9I GGNCC 2 cut(s) 317, 443
AsuHPI GGTGA 2 cut(s) 145, 198
AvaII GGWCC 1 cut(s) 443
BaeGI GKGCMC 1 cut(s) 513
BbvI GCAGC 1 cut(s) 245
BccI CCATC 1 cut(s) 719
BfaI CTAG 1 cut(s) 321
BfmI CTRYAG 2 cut(s) 231, 417
BfuAI ACCTGC 1 cut(s) 286
BisI GCNGC 2 cut(s) 234, 531
BlsI GCNGC 2 cut(s) 235, 532
Bme18I GGWCC 1 cut(s) 443
BmeRI GACNNNNNGTC 1 cut(s) 568
BmgT120I GGNCC 2 cut(s) 317, 443
BmrI ACTGGG 1 cut(s) 469
BmsI GCATC 5 cut(s) 55, 348, 632, 687, 719
BmuI ACTGGG 1 cut(s) 469
BpmI CTGGAG 1 cut(s) 501
BsaJI CCNNGG 1 cut(s) 534
Bsc4I CCNNNNNNNGG 3 cut(s) 314, 440, 479
Bse1I ACTGG 6 cut(s) 161, 282, 445, 475, 650, 733
Bse3DI GCAATG 1 cut(s) 221
BseDI CCNNGG 1 cut(s) 534
BseGI GGATG 2 cut(s) 27, 734
BseLI CCNNNNNNNGG 3 cut(s) 314, 440, 479
BseMI GCAATG 1 cut(s) 221
BseNI ACTGG 6 cut(s) 161, 282, 445, 475, 650, 733
BseRI GAGGAG 1 cut(s) 447
BseSI GKGCMC 1 cut(s) 513
BseXI GCAGC 1 cut(s) 245
Bsh1236I CGCG 1 cut(s) 545
Bsh1285I CGRYCG 1 cut(s) 507
BshFI GGCC 3 cut(s) 319, 533, 665
BsiEI CGRYCG 1 cut(s) 507
BsiSI CCGG 1 cut(s) 666
BslFI GGGAC 3 cut(s) 224, 685, 703
BslI CCNNNNNNNGG 3 cut(s) 314, 440, 479
BsmFI GGGAC 3 cut(s) 224, 685, 703
BsnI GGCC 3 cut(s) 319, 533, 665
Bsp1286I GDGCHC 1 cut(s) 513
Bsp143I GATC 2 cut(s) 587, 712
Bsp19I CCATGG 1 cut(s) 534
BspACI CCGC 2 cut(s) 530, 549
BspANI GGCC 3 cut(s) 319, 533, 665
BspFNI CGCG 1 cut(s) 545
BspMAI CTGCAG 1 cut(s) 235
BspMI ACCTGC 1 cut(s) 286
BspPI GGATC 2 cut(s) 595, 707
BsrDI GCAATG 1 cut(s) 221
BsrI ACTGG 6 cut(s) 161, 282, 445, 475, 650, 733
BssECI CCNNGG 1 cut(s) 534
BssMI GATC 2 cut(s) 587, 712
BssT1I CCWWGG 1 cut(s) 534
Bst4CI ACNGT 2 cut(s) 421, 508
BstAPI GCANNNNNTGC 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 175, 547
BstDEI CTNAG 1 cut(s) 347
BstDSI CCRYGG 1 cut(s) 534
BstF5I GGATG 2 cut(s) 27, 734
BstFNI CGCG 1 cut(s) 545
BstKTI GATC 2 cut(s) 590, 715
BstMBI GATC 2 cut(s) 587, 712
BstMCI CGRYCG 1 cut(s) 507
BstMWI GCNNNNNNNGC 2 cut(s) 508, 629
BstSFI CTRYAG 2 cut(s) 231, 417
BstSLI GKGCMC 1 cut(s) 513
BstUI CGCG 1 cut(s) 545
BstV1I GCAGC 1 cut(s) 245
BsuRI GGCC 3 cut(s) 319, 533, 665
BtgI CCRYGG 1 cut(s) 534
BtgZI GCGATG 1 cut(s) 710
BtsCI GGATG 2 cut(s) 27, 734
BtsI GCAGTG 1 cut(s) 705
BtsIMutI CAGTG 1 cut(s) 705
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 2 cut(s) 175, 547
Cfr13I GGNCC 2 cut(s) 317, 443
Csp6I GTAC 2 cut(s) 111, 167
CviAII CATG 2 cut(s) 535, 620
CviQI GTAC 2 cut(s) 111, 167
DdeI CTNAG 1 cut(s) 347
DpnI GATC 2 cut(s) 589, 714
DpnII GATC 2 cut(s) 587, 712
DrdI GACNNNNNNGTC 1 cut(s) 521
DriI GACNNNNNGTC 1 cut(s) 568
DseDI GACNNNNNNGTC 1 cut(s) 521
EaeI YGGCCR 2 cut(s) 531, 663
Eam1105I GACNNNNNGTC 1 cut(s) 568
Eco130I CCWWGG 1 cut(s) 534
Eco47I GGWCC 1 cut(s) 443
Eco57I CTGAAG 1 cut(s) 146
EcoT14I CCWWGG 1 cut(s) 534
EcoT22I ATGCAT 1 cut(s) 625
ErhI CCWWGG 1 cut(s) 534
FaeI CATG 2 cut(s) 538, 623
FaiI YATR 8 cut(s) 61, 152, 158, 301, 380, 536, 621, 630
FaqI GGGAC 3 cut(s) 224, 685, 703
FatI CATG 2 cut(s) 534, 619
FauI CCCGC 1 cut(s) 542
Fnu4HI GCNGC 2 cut(s) 234, 531
FokI GGATG 2 cut(s) 14, 741
Fsp4HI GCNGC 2 cut(s) 234, 531
FspBI CTAG 1 cut(s) 321
GluI GCNGC 2 cut(s) 234, 531
GsuI CTGGAG 1 cut(s) 501
HaeIII GGCC 3 cut(s) 319, 533, 665
HapII CCGG 1 cut(s) 666
Hin1II CATG 2 cut(s) 538, 623
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 2 cut(s) 5, 203
HpaII CCGG 1 cut(s) 666
HphI GGTGA 2 cut(s) 145, 198
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 1 cut(s) 202
Hpy188III TCNNGA 1 cut(s) 480
Hpy8I GTNNAC 1 cut(s) 424
HpyAV CCTTC 4 cut(s) 124, 207, 648, 652
HpyCH4III ACNGT 2 cut(s) 421, 508
HpyCH4IV ACGT 1 cut(s) 608
HpyCH4V TGCA 5 cut(s) 68, 233, 339, 567, 623
HpyF10VI GCNNNNNNNGC 2 cut(s) 508, 629
HpyF3I CTNAG 1 cut(s) 347
HpySE526I ACGT 1 cut(s) 608
Hsp92II CATG 2 cut(s) 538, 623
Kzo9I GATC 2 cut(s) 587, 712
Lsp1109I GCAGC 1 cut(s) 245
LweI GCATC 5 cut(s) 55, 348, 632, 687, 719
MaeI CTAG 1 cut(s) 321
MaeII ACGT 1 cut(s) 608
MaeIII GTNAC 2 cut(s) 139, 703
MalI GATC 2 cut(s) 589, 714
MboI GATC 2 cut(s) 587, 712
MboII GAAGA 4 cut(s) 139, 180, 242, 730
MhlI GDGCHC 1 cut(s) 513
MluCI AATT 1 cut(s) 640
MluI ACGCGT 1 cut(s) 543
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 8 cut(s) 41, 252, 318, 396, 421, 468, 662, 680
Mph1103I ATGCAT 1 cut(s) 625
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 1 cut(s) 666
MvnI CGCG 1 cut(s) 545
MwoI GCNNNNNNNGC 2 cut(s) 508, 629
NcoI CCATGG 1 cut(s) 534
NdeII GATC 2 cut(s) 587, 712
NlaIII CATG 2 cut(s) 538, 623
NmuCI GTSAC 1 cut(s) 703
NsiI ATGCAT 1 cut(s) 625
PaqCI CACCTGC 1 cut(s) 286
PfeI GAWTC 1 cut(s) 203
PflMI CCANNNNNTGG 1 cut(s) 440
PkrI GCNGC 2 cut(s) 235, 532
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PspPI GGNCC 2 cut(s) 317, 443
PstI CTGCAG 1 cut(s) 235
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 2 cut(s) 112, 168
RsaNI GTAC 2 cut(s) 111, 167
SatI GCNGC 2 cut(s) 234, 531
Sau3AI GATC 2 cut(s) 587, 712
Sau96I GGNCC 2 cut(s) 317, 443
SchI GAGTC 1 cut(s) 14
SduI GDGCHC 1 cut(s) 513
SetI ASST 9 cut(s) 49, 135, 179, 199, 238, 280, 310, 611, 663
SfaNI GCATC 5 cut(s) 55, 348, 632, 687, 719
SfcI CTRYAG 2 cut(s) 231, 417
SinI GGWCC 1 cut(s) 443
Sse9I AATT 1 cut(s) 640
SsiI CCGC 2 cut(s) 530, 549
SspMI CTAG 1 cut(s) 321
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 2 cut(s) 421, 508
TaiI ACGT 1 cut(s) 611
TasI AATT 1 cut(s) 640
TatI WGTACW 2 cut(s) 110, 166
TauI GCSGC 1 cut(s) 533
TfiI GAWTC 1 cut(s) 203
TscAI CASTG 1 cut(s) 712
TseFI GTSAC 1 cut(s) 703
TseI GCWGC 1 cut(s) 233
Tsp45I GTSAC 1 cut(s) 703
TspGWI ACGGA 1 cut(s) 600
TspRI CASTG 1 cut(s) 712
Van91I CCANNNNNTGG 1 cut(s) 440
VpaK11BI GGWCC 1 cut(s) 443
XspI CTAG 1 cut(s) 321
Zsp2I ATGCAT 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.