RLG00000021278

Cytochrome b6-f complex iron-sulfur subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003668
Physical Location & Seq
Forward (+)
73683180 .. 73684562
1383 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021278

Sequence Viewer

Length: 681 bp
ATGGCTGCCTCTACTCTATCTTCTGCTACCACTTCACAGCTATGCTCCAGCAAGAGTGCTATGTTCTCTTCAGCACAGGGCCTGTCCGTGATGCCCAGGAGGACCCAGATGATGGGAAAGGGGAAGGGAATGAAAATCACATGCCAGGCAGCAATTCCAGCTGATAATGTCCCTGACATGGGTAAGAGGAAGCTTATGAATTTGCTTCTTCTGGGTGCTATCTCACTTCCCTCTGGTTTCATGCTGGTTCCGTATGCTACTTTCTTTGCTCCACCTGGCTCTGGTGCTGGAAGTGGTGGTCAAGTTGCCAAGGATGCTATCGGAAACGATGTAATTGCATCAGAATGGCTTAAGACCCATGGCCCTGGTGACAGGACTCTCACACAAGGATTGAAGGGTGATCCTACCTACCTTGTTGTGGAGAAAGACAGAACTCTTGCCACATATGGAATCAATGCAGTATGCACTCACCTTGGTTGTGTCGTGCCATTCAACACTGCCGAGAACAAGTTCATCTGCCCATGCCATGGATCCCAGTACAATGACCAAGGAAGGGTTGTCAGGGGACCTGCACCTCTGTCTCTGGCTTTGGCTCATGCTGATATTGATGAAGGCAAGGTATTATTTGTTCCTTGGGTCGAAACCGATTTCAGAACCGGTGAGGATCCATGGTGGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
CytB6-F_Fe-S Rieske

Protein Analysis

227

Amino Acids

23.92

Weight (kDa)

8.21

Isoelectric Point (pI)

23.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TM_PetC PF25471 54 - 101 7.8e-23 Iron-sulfur subunit PetC transmembrane anchor
Rieske PF00355 133 - 193 5.9e-10 Rieske [2Fe-2S] domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 577
AccB7I CCANNNNNTGG 2 cut(s) 112, 527
AclWI GGATC 5 cut(s) 395, 525, 538, 659, 672
AcsI RAATTY 1 cut(s) 199
AcuI CTGAAG 1 cut(s) 54
AfaI GTAC 1 cut(s) 539
AfiI CCNNNNNNNGG 7 cut(s) 112, 178, 179, 281, 418, 527, 553
AflII CTTAAG 1 cut(s) 350
AgeI ACCGGT 1 cut(s) 656
AgsI TTSAA 2 cut(s) 394, 493
AjnI CCWGG 4 cut(s) 95, 144, 274, 364
AluBI AGCT 3 cut(s) 40, 161, 193
AluI AGCT 3 cut(s) 40, 161, 193
Alw26I GTCTC 1 cut(s) 585
AlwI GGATC 5 cut(s) 395, 525, 538, 659, 672
AlwNI CAGNNNCTG 1 cut(s) 82
AoxI GGCC 2 cut(s) 79, 361
ApeKI GCWGC 2 cut(s) 5, 149
ApoI RAATTY 1 cut(s) 199
AsiGI ACCGGT 1 cut(s) 656
Asp700I GAANNNNTTC 1 cut(s) 509
AspS9I GGNCC 4 cut(s) 79, 102, 362, 566
AsuHPI GGTGA 4 cut(s) 380, 410, 461, 671
AvaII GGWCC 2 cut(s) 102, 566
BamHI GGATCC 2 cut(s) 530, 664
BbvI GCAGC 1 cut(s) 161
BccI CCATC 1 cut(s) 106
BcgI CGANNNNNNTGC 2 cut(s) 317, 351
BciT130I CCWGG 4 cut(s) 97, 146, 276, 366
BcoDI GTCTC 1 cut(s) 585
BfrI CTTAAG 1 cut(s) 350
BfuAI ACCTGC 1 cut(s) 577
BisI GCNGC 2 cut(s) 6, 150
BlsI GCNGC 2 cut(s) 7, 151
Bme1390I CCNGG 4 cut(s) 97, 146, 276, 366
Bme18I GGWCC 2 cut(s) 102, 566
BmgT120I GGNCC 4 cut(s) 79, 102, 362, 566
BmiI GGNNCC 5 cut(s) 104, 249, 532, 567, 666
BmrFI CCNGG 4 cut(s) 97, 146, 276, 366
BmrI ACTGGG 1 cut(s) 529
BmsI GCATC 3 cut(s) 81, 304, 347
BmuI ACTGGG 1 cut(s) 529
BpmI CTGGAG 1 cut(s) 31
BsaJI CCNNGG 9 cut(s) 95, 309, 358, 364, 472, 526, 547, 632, 668
BsaWI WCCGGW 1 cut(s) 656
Bsc4I CCNNNNNNNGG 7 cut(s) 112, 178, 179, 281, 418, 527, 553
Bse118I RCCGGY 1 cut(s) 656
Bse1I ACTGG 1 cut(s) 535
BseBI CCWGG 4 cut(s) 97, 146, 276, 366
BseDI CCNNGG 9 cut(s) 95, 309, 358, 364, 472, 526, 547, 632, 668
BseGI GGATG 1 cut(s) 319
BseLI CCNNNNNNNGG 7 cut(s) 112, 178, 179, 281, 418, 527, 553
BseNI ACTGG 1 cut(s) 535
BseXI GCAGC 1 cut(s) 161
BsgI GTGCAG 1 cut(s) 555
BshFI GGCC 2 cut(s) 81, 363
BshTI ACCGGT 1 cut(s) 656
BsiSI CCGG 1 cut(s) 657
BslFI GGGAC 2 cut(s) 155, 579
BslI CCNNNNNNNGG 7 cut(s) 112, 178, 179, 281, 418, 527, 553
BsmAI GTCTC 1 cut(s) 585
BsmFI GGGAC 2 cut(s) 155, 579
BsnI GGCC 2 cut(s) 81, 363
Bsp143I GATC 3 cut(s) 400, 530, 664
Bsp19I CCATGG 3 cut(s) 358, 526, 668
BspANI GGCC 2 cut(s) 81, 363
BspLI GGNNCC 5 cut(s) 104, 249, 532, 567, 666
BspMI ACCTGC 1 cut(s) 577
BspPI GGATC 5 cut(s) 395, 525, 538, 659, 672
BspTI CTTAAG 1 cut(s) 350
BsrFI RCCGGY 1 cut(s) 656
BsrI ACTGG 1 cut(s) 535
BssAI RCCGGY 1 cut(s) 656
BssECI CCNNGG 9 cut(s) 95, 309, 358, 364, 472, 526, 547, 632, 668
BssMI GATC 3 cut(s) 400, 530, 664
BssT1I CCWWGG 7 cut(s) 309, 358, 472, 526, 547, 632, 668
Bst2UI CCWGG 4 cut(s) 97, 146, 276, 366
Bst6I CTCTTC 1 cut(s) 73
BstAFI CTTAAG 1 cut(s) 350
BstDSI CCRYGG 3 cut(s) 358, 526, 668
BstF5I GGATG 1 cut(s) 319
BstKTI GATC 3 cut(s) 403, 533, 667
BstMAI GTCTC 1 cut(s) 585
BstMBI GATC 3 cut(s) 400, 530, 664
BstMWI GCNNNNNNNGC 2 cut(s) 158, 314
BstNI CCWGG 4 cut(s) 97, 146, 276, 366
BstNSI RCATGY 1 cut(s) 144
BstSCI CCNGG 4 cut(s) 95, 144, 274, 364
BstV1I GCAGC 1 cut(s) 161
BstX2I RGATCY 2 cut(s) 530, 664
BstXI CCANNNNNNTGG 1 cut(s) 365
BstYI RGATCY 2 cut(s) 530, 664
BsuRI GGCC 2 cut(s) 81, 363
BtgI CCRYGG 3 cut(s) 358, 526, 668
BtsCI GGATG 1 cut(s) 319
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 1 cut(s) 495
BveI ACCTGC 1 cut(s) 577
CaiI CAGNNNCTG 1 cut(s) 82
Cfr10I RCCGGY 1 cut(s) 656
Cfr13I GGNCC 4 cut(s) 79, 102, 362, 566
Csp6I GTAC 1 cut(s) 538
CspAI ACCGGT 1 cut(s) 656
CviAII CATG 8 cut(s) 141, 178, 241, 359, 522, 527, 596, 669
CviQI GTAC 1 cut(s) 538
DpnI GATC 3 cut(s) 402, 532, 666
DpnII GATC 3 cut(s) 400, 530, 664
Eam1104I CTCTTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 73
Eco130I CCWWGG 7 cut(s) 309, 358, 472, 526, 547, 632, 668
Eco47I GGWCC 2 cut(s) 102, 566
Eco57I CTGAAG 1 cut(s) 54
EcoO109I RGGNCCY 3 cut(s) 79, 102, 566
EcoRII CCWGG 4 cut(s) 95, 144, 274, 364
EcoT14I CCWWGG 7 cut(s) 309, 358, 472, 526, 547, 632, 668
ErhI CCWWGG 7 cut(s) 309, 358, 472, 526, 547, 632, 668
FaeI CATG 8 cut(s) 144, 181, 244, 362, 525, 530, 599, 672
FaqI GGGAC 2 cut(s) 155, 579
FatI CATG 8 cut(s) 140, 177, 240, 358, 521, 526, 595, 668
FauNDI CATATG 1 cut(s) 445
Fnu4HI GCNGC 2 cut(s) 6, 150
FokI GGATG 1 cut(s) 326
Fsp4HI GCNGC 2 cut(s) 6, 150
GluI GCNGC 2 cut(s) 6, 150
GsuI CTGGAG 1 cut(s) 31
HaeIII GGCC 2 cut(s) 81, 363
HapII CCGG 1 cut(s) 657
Hin1II CATG 8 cut(s) 144, 181, 244, 362, 525, 530, 599, 672
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 2 cut(s) 376, 450
HpaII CCGG 1 cut(s) 657
HphI GGTGA 4 cut(s) 380, 410, 461, 671
Hpy188I TCNGA 3 cut(s) 323, 343, 653
HpyAV CCTTC 4 cut(s) 118, 388, 546, 605
HpyCH4V TGCA 4 cut(s) 338, 458, 465, 572
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 314
Hsp92II CATG 8 cut(s) 144, 181, 244, 362, 525, 530, 599, 672
Kzo9I GATC 3 cut(s) 400, 530, 664
LmnI GCTCC 2 cut(s) 50, 274
Lsp1109I GCAGC 1 cut(s) 161
LweI GCATC 3 cut(s) 81, 304, 347
MaeIII GTNAC 1 cut(s) 368
MalI GATC 3 cut(s) 402, 532, 666
MboI GATC 3 cut(s) 400, 530, 664
MboII GAAGA 3 cut(s) 12, 60, 200
MflI RGATCY 2 cut(s) 530, 664
MluCI AATT 3 cut(s) 153, 199, 333
MlyI GAGTC 1 cut(s) 370
MnlI CCTC 6 cut(s) 19, 93, 180, 241, 585, 655
MroXI GAANNNNTTC 1 cut(s) 509
MseI TTAA 2 cut(s) 351, 679
MslI CAYNNNNRTG 2 cut(s) 40, 343
MspA1I CMGCKG 1 cut(s) 161
MspCI CTTAAG 1 cut(s) 350
MspI CCGG 1 cut(s) 657
MspR9I CCNGG 4 cut(s) 97, 146, 276, 366
MvaI CCWGG 4 cut(s) 97, 146, 276, 366
MwoI GCNNNNNNNGC 2 cut(s) 158, 314
NcoI CCATGG 3 cut(s) 358, 526, 668
NdeI CATATG 1 cut(s) 445
NdeII GATC 3 cut(s) 400, 530, 664
NlaIII CATG 8 cut(s) 144, 181, 244, 362, 525, 530, 599, 672
NlaIV GGNNCC 5 cut(s) 104, 249, 532, 567, 666
NmeAIII GCCGAG 1 cut(s) 526
NmuCI GTSAC 1 cut(s) 368
NspI RCATGY 1 cut(s) 144
PdmI GAANNNNTTC 1 cut(s) 509
PfeI GAWTC 1 cut(s) 450
PflMI CCANNNNNTGG 2 cut(s) 112, 527
PinAI ACCGGT 1 cut(s) 656
PkrI GCNGC 2 cut(s) 7, 151
PleI GAGTC 1 cut(s) 370
PpsI GAGTC 1 cut(s) 370
PpuMI RGGWCCY 2 cut(s) 102, 566
Psp5II RGGWCCY 2 cut(s) 102, 566
Psp6I CCWGG 4 cut(s) 95, 144, 274, 364
PspGI CCWGG 4 cut(s) 95, 144, 274, 364
PspN4I GGNNCC 5 cut(s) 104, 249, 532, 567, 666
PspPI GGNCC 4 cut(s) 79, 102, 362, 566
PspPPI RGGWCCY 2 cut(s) 102, 566
PsrI GAACNNNNNNTAC 2 cut(s) 612, 644
PstNI CAGNNNCTG 1 cut(s) 82
PsuI RGATCY 2 cut(s) 530, 664
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
RseI CAYNNNNRTG 2 cut(s) 40, 343
SaqAI TTAA 2 cut(s) 351, 679
SatI GCNGC 2 cut(s) 6, 150
Sau3AI GATC 3 cut(s) 400, 530, 664
Sau96I GGNCC 4 cut(s) 79, 102, 362, 566
SchI GAGTC 1 cut(s) 370
ScrFI CCNGG 4 cut(s) 97, 146, 276, 366
SfaNI GCATC 3 cut(s) 81, 304, 347
SinI GGWCC 2 cut(s) 102, 566
SmiMI CAYNNNNRTG 2 cut(s) 40, 343
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
Sse9I AATT 3 cut(s) 153, 199, 333
StyD4I CCNGG 4 cut(s) 95, 144, 274, 364
StyI CCWWGG 7 cut(s) 309, 358, 472, 526, 547, 632, 668
TaqI TCGA 1 cut(s) 639
TasI AATT 3 cut(s) 153, 199, 333
TatI WGTACW 1 cut(s) 537
TfiI GAWTC 1 cut(s) 450
Tru1I TTAA 2 cut(s) 351, 679
Tru9I TTAA 2 cut(s) 351, 679
TscAI CASTG 1 cut(s) 502
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 2 cut(s) 5, 149
Tsp45I GTSAC 1 cut(s) 368
TspDTI ATGAA 5 cut(s) 146, 212, 229, 502, 624
TspGWI ACGGA 2 cut(s) 76, 240
TspRI CASTG 1 cut(s) 502
Van91I CCANNNNNTGG 2 cut(s) 112, 527
Vha464I CTTAAG 1 cut(s) 350
VpaK11BI GGWCC 2 cut(s) 102, 566
XapI RAATTY 1 cut(s) 199
XceI RCATGY 1 cut(s) 144
XmnI GAANNNNTTC 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.