Rh6BG115100

Cytochrome b6-f complex iron-sulfur subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0003668
Physical Location & Seq
Forward (+)
17914574 .. 17934492
19919 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG115100.1

Sequence Viewer

Length: 861 bp
ATGTTTCAGCTATGCTCCAACAAGAGTGTCATGTTCTCTTCAGCACAGGCTCTGTCTCTGATGCCCAGGAGGACCCAGATGATGGGAAAGGGAAAGGGAATGAGAATCACATGCCAAGCTGCAATTCCAGCTGATAATATCCCTGACATGGGTAAGAGGAAGCTTATGAATCTGCTTCTTCTAACGAATCTCACTTCCCTCTGGTTTCCTATGCCACTTTGTTTGCTCCACCTGGGAAGTGGTGGTCAAGTTGCCAAGGATGCTCTAGGAAATGATGTAATTGCAGCAGAATGGCTTAAGAACCATGGCCCTGGTGACAGGACTTTCACACAAGGATTGAAGGATGGTCTTTGCTATGGGAAGTTGGAAGCTGGACTACTTACATTCATTATACCCATTGTTCTTCTTGGGCACCTGGGAGCACTTAGAGTTTCCATGACAAGTCCAGGATTTATCTATGAGCCTTATGCACCTAGTGAAAATGTTCCATTTTTCCGGAGATTCCTTTTGAGCTCGAGCTCTCTCTCTTCTTCTCCTCCTCCCTTTGGCTTTGGCTTTGCTCTCTTTGCTGTTCTAAGCAGGGAAGTCTTACATCTGTTTTTGCGGGGGGACTCCCGACAGTTCAACTCAAGCGGAAAAATGATTAAGCTATTTAAAGTGAAGGAAAAGCAGAGGGAGCTTGCTGAAAATGCCAATGGAAAGACACTGGTTAAGAAGCAAAGCGCAGGAGAATTACGGCTTCATAGAGATATTAGTGAGCTGAACCTACAGAAAACATGTACCATAGCATTCCCCAATGGTAAGGACGATTTAATGAACTTTGAGGTTACCGTCCGGCCTGATGAAGGATACTATCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
CytB6-F_Fe-S Rieske

Protein Analysis

286

Amino Acids

31.65

Weight (kDa)

9.53

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2301 PF10063 113 - 142 1.4e-06 Uncharacterized integral membrane protein (DUF2301)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 411
AccB7I CCANNNNNTGG 1 cut(s) 82
AccIII TCCGGA 1 cut(s) 495
AciI CCGC 2 cut(s) 604, 633
AcuI CTGAAG 1 cut(s) 24
AdeI CACNNNGTG 1 cut(s) 476
AfaI GTAC 1 cut(s) 781
AfiI CCNNNNNNNGG 5 cut(s) 82, 148, 149, 545, 845
AflII CTTAAG 1 cut(s) 296
AflIII ACRYGT 1 cut(s) 776
AgsI TTSAA 2 cut(s) 340, 625
AjnI CCWGG 5 cut(s) 65, 231, 310, 414, 445
AjuI GAANNNNNNNTTGG 2 cut(s) 108, 140
Alw21I GWGCWC 3 cut(s) 424, 515, 521
Alw26I GTCTC 1 cut(s) 60
AlwNI CAGNNNCTG 1 cut(s) 52
Ama87I CYCGRG 1 cut(s) 514
Aor13HI TCCGGA 1 cut(s) 495
AoxI GGCC 2 cut(s) 307, 836
ApeKI GCWGC 2 cut(s) 119, 284
Asp700I GAANNNNTTC 1 cut(s) 483
AspLEI GCGC 1 cut(s) 725
AspS9I GGNCC 2 cut(s) 72, 308
AsuHPI GGTGA 1 cut(s) 326
AvaI CYCGRG 1 cut(s) 514
AvaII GGWCC 1 cut(s) 72
BaeGI GKGCMC 1 cut(s) 414
BanI GGYRCC 1 cut(s) 411
BanII GRGCYC 2 cut(s) 515, 521
BarI GAAGNNNNNNTAC 2 cut(s) 360, 392
Bbv12I GWGCWC 3 cut(s) 424, 515, 521
BbvI GCAGC 2 cut(s) 106, 296
BccI CCATC 2 cut(s) 76, 338
BceAI ACGGC 1 cut(s) 752
BciT130I CCWGG 5 cut(s) 67, 233, 312, 416, 447
BciVI GTATCC 1 cut(s) 842
BcoDI GTCTC 1 cut(s) 60
BfaI CTAG 2 cut(s) 266, 474
BfmI CTRYAG 1 cut(s) 767
BfrI CTTAAG 1 cut(s) 296
BfuI GTATCC 1 cut(s) 842
BisI GCNGC 2 cut(s) 120, 285
BlsI GCNGC 2 cut(s) 121, 286
Bme1390I CCNGG 5 cut(s) 67, 233, 312, 416, 447
Bme18I GGWCC 1 cut(s) 72
BmeT110I CYCGRG 1 cut(s) 514
BmgT120I GGNCC 2 cut(s) 72, 308
BmiI GGNNCC 2 cut(s) 74, 413
BmrFI CCNGG 5 cut(s) 67, 233, 312, 416, 447
BmsI GCATC 2 cut(s) 51, 250
BpuEI CTTGAG 1 cut(s) 613
BsaJI CCNNGG 6 cut(s) 65, 232, 255, 304, 310, 415
BsaWI WCCGGW 1 cut(s) 495
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 148, 149, 545, 845
Bse1I ACTGG 1 cut(s) 711
BseAI TCCGGA 1 cut(s) 495
BseBI CCWGG 5 cut(s) 67, 233, 312, 416, 447
BseDI CCNNGG 6 cut(s) 65, 232, 255, 304, 310, 415
BseGI GGATG 2 cut(s) 265, 349
BseLI CCNNNNNNNGG 5 cut(s) 82, 148, 149, 545, 845
BseNI ACTGG 1 cut(s) 711
BseRI GAGGAG 2 cut(s) 525, 528
BseSI GKGCMC 1 cut(s) 414
BseXI GCAGC 2 cut(s) 106, 296
BshFI GGCC 2 cut(s) 309, 838
BshNI GGYRCC 1 cut(s) 411
BsiHKAI GWGCWC 3 cut(s) 424, 515, 521
BsiHKCI CYCGRG 1 cut(s) 514
BsiSI CCGG 2 cut(s) 496, 835
BslFI GGGAC 1 cut(s) 623
BslI CCNNNNNNNGG 5 cut(s) 82, 148, 149, 545, 845
BsmAI GTCTC 1 cut(s) 60
BsmFI GGGAC 1 cut(s) 623
BsmI GAATGC 1 cut(s) 788
BsnI GGCC 2 cut(s) 309, 838
BsoBI CYCGRG 1 cut(s) 514
Bsp1286I GDGCHC 4 cut(s) 414, 424, 515, 521
Bsp13I TCCGGA 1 cut(s) 495
Bsp19I CCATGG 1 cut(s) 304
BspACI CCGC 2 cut(s) 604, 633
BspANI GGCC 2 cut(s) 309, 838
BspEI TCCGGA 1 cut(s) 495
BspLI GGNNCC 2 cut(s) 74, 413
BspT107I GGYRCC 1 cut(s) 411
BspTI CTTAAG 1 cut(s) 296
BsrI ACTGG 1 cut(s) 711
BssECI CCNNGG 6 cut(s) 65, 232, 255, 304, 310, 415
BssT1I CCWWGG 2 cut(s) 255, 304
Bst2UI CCWGG 5 cut(s) 67, 233, 312, 416, 447
Bst4CI ACNGT 2 cut(s) 621, 832
Bst6I CTCTTC 2 cut(s) 43, 532
BstAFI CTTAAG 1 cut(s) 296
BstC8I GCNNGC 1 cut(s) 681
BstDEI CTNAG 2 cut(s) 425, 575
BstDSI CCRYGG 1 cut(s) 304
BstEII GGTNACC 1 cut(s) 826
BstENI CCTNNNNNAGG 1 cut(s) 843
BstF5I GGATG 2 cut(s) 265, 349
BstHHI GCGC 1 cut(s) 725
BstMAI GTCTC 1 cut(s) 60
BstMWI GCNNNNNNNGC 5 cut(s) 128, 260, 566, 676, 689
BstNI CCWGG 5 cut(s) 67, 233, 312, 416, 447
BstNSI RCATGY 2 cut(s) 114, 780
BstPI GGTNACC 1 cut(s) 826
BstSCI CCNGG 5 cut(s) 65, 231, 310, 414, 445
BstSFI CTRYAG 1 cut(s) 767
BstSLI GKGCMC 1 cut(s) 414
BstV1I GCAGC 2 cut(s) 106, 296
BstXI CCANNNNNNTGG 1 cut(s) 311
BsuI GTATCC 1 cut(s) 842
BsuRI GGCC 2 cut(s) 309, 838
BtgI CCRYGG 1 cut(s) 304
BtsCI GGATG 2 cut(s) 265, 349
BtsIMutI CAGTG 1 cut(s) 704
Cac8I GCNNGC 1 cut(s) 681
CaiI CAGNNNCTG 1 cut(s) 52
CfoI GCGC 1 cut(s) 725
Cfr13I GGNCC 2 cut(s) 72, 308
Csp6I GTAC 1 cut(s) 780
CspCI CAANNNNNGTGG 2 cut(s) 204, 239
CviAII CATG 6 cut(s) 31, 111, 148, 305, 436, 777
CviQI GTAC 1 cut(s) 780
DdeI CTNAG 2 cut(s) 425, 575
DraI TTTAAA 1 cut(s) 655
DraIII CACNNNGTG 1 cut(s) 476
Eam1104I CTCTTC 2 cut(s) 43, 532
EarI CTCTTC 2 cut(s) 43, 532
Ecl136II GAGCTC 2 cut(s) 513, 519
Eco130I CCWWGG 2 cut(s) 255, 304
Eco24I GRGCYC 2 cut(s) 515, 521
Eco47I GGWCC 1 cut(s) 72
Eco53kI GAGCTC 2 cut(s) 513, 519
Eco57I CTGAAG 1 cut(s) 24
Eco88I CYCGRG 1 cut(s) 514
Eco91I GGTNACC 1 cut(s) 826
EcoICRI GAGCTC 2 cut(s) 513, 519
EcoNI CCTNNNNNAGG 1 cut(s) 843
EcoO109I RGGNCCY 1 cut(s) 72
EcoO65I GGTNACC 1 cut(s) 826
EcoRII CCWGG 5 cut(s) 65, 231, 310, 414, 445
EcoT14I CCWWGG 2 cut(s) 255, 304
EcoT38I GRGCYC 2 cut(s) 515, 521
ErhI CCWWGG 2 cut(s) 255, 304
FaeI CATG 6 cut(s) 34, 114, 151, 308, 439, 780
FaqI GGGAC 1 cut(s) 623
FatI CATG 6 cut(s) 30, 110, 147, 304, 435, 776
FauI CCCGC 1 cut(s) 597
Fnu4HI GCNGC 2 cut(s) 120, 285
FokI GGATG 2 cut(s) 272, 356
FriOI GRGCYC 2 cut(s) 515, 521
Fsp4HI GCNGC 2 cut(s) 120, 285
FspBI CTAG 2 cut(s) 266, 474
GlaI GCGC 1 cut(s) 724
GluI GCNGC 2 cut(s) 120, 285
HaeIII GGCC 2 cut(s) 309, 838
HapII CCGG 2 cut(s) 496, 835
HhaI GCGC 1 cut(s) 725
Hin1II CATG 6 cut(s) 34, 114, 151, 308, 439, 780
Hin6I GCGC 1 cut(s) 723
HinP1I GCGC 1 cut(s) 723
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 5 cut(s) 105, 169, 187, 501, 611
HpaII CCGG 2 cut(s) 496, 835
HphI GGTGA 1 cut(s) 326
Hpy188I TCNGA 1 cut(s) 60
Hpy188III TCNNGA 2 cut(s) 496, 615
HpyAV CCTTC 3 cut(s) 334, 655, 839
HpyCH4III ACNGT 2 cut(s) 621, 832
HpyCH4V TGCA 3 cut(s) 122, 284, 470
HpyF10VI GCNNNNNNNGC 5 cut(s) 128, 260, 566, 676, 689
HpyF3I CTNAG 2 cut(s) 425, 575
Hsp92II CATG 6 cut(s) 34, 114, 151, 308, 439, 780
HspAI GCGC 1 cut(s) 723
Kpn2I TCCGGA 1 cut(s) 495
LmnI GCTCC 4 cut(s) 20, 231, 419, 676
Lsp1109I GCAGC 2 cut(s) 106, 296
LweI GCATC 2 cut(s) 51, 250
MaeI CTAG 2 cut(s) 266, 474
MaeIII GTNAC 2 cut(s) 314, 826
MboII GAAGA 5 cut(s) 30, 170, 395, 519, 522
MhlI GDGCHC 4 cut(s) 414, 424, 515, 521
MluCI AATT 3 cut(s) 123, 279, 731
MlyI GAGTC 1 cut(s) 605
MmeI TCCRAC 2 cut(s) 42, 345
MnlI CCTC 7 cut(s) 63, 150, 209, 546, 549, 666, 817
MroI TCCGGA 1 cut(s) 495
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 5 cut(s) 297, 645, 654, 711, 812
MspA1I CMGCKG 1 cut(s) 131
MspCI CTTAAG 1 cut(s) 296
MspI CCGG 2 cut(s) 496, 835
MspR9I CCNGG 5 cut(s) 67, 233, 312, 416, 447
Mva1269I GAATGC 1 cut(s) 788
MvaI CCWGG 5 cut(s) 67, 233, 312, 416, 447
MwoI GCNNNNNNNGC 5 cut(s) 128, 260, 566, 676, 689
NcoI CCATGG 1 cut(s) 304
NlaIII CATG 6 cut(s) 34, 114, 151, 308, 439, 780
NlaIV GGNNCC 2 cut(s) 74, 413
NmuCI GTSAC 1 cut(s) 314
NspI RCATGY 2 cut(s) 114, 780
PaeR7I CTCGAG 1 cut(s) 514
PciI ACATGT 1 cut(s) 776
PctI GAATGC 1 cut(s) 788
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 4 cut(s) 105, 169, 187, 501
PflMI CCANNNNNTGG 1 cut(s) 82
PfoI TCCNGGA 1 cut(s) 445
PkrI GCNGC 2 cut(s) 121, 286
PleI GAGTC 1 cut(s) 605
PpsI GAGTC 1 cut(s) 605
PpuMI RGGWCCY 1 cut(s) 72
PscI ACATGT 1 cut(s) 776
Psp124BI GAGCTC 2 cut(s) 515, 521
Psp5II RGGWCCY 1 cut(s) 72
Psp6I CCWGG 5 cut(s) 65, 231, 310, 414, 445
PspEI GGTNACC 1 cut(s) 826
PspGI CCWGG 5 cut(s) 65, 231, 310, 414, 445
PspN4I GGNNCC 2 cut(s) 74, 413
PspPI GGNCC 2 cut(s) 72, 308
PspPPI RGGWCCY 1 cut(s) 72
PspXI VCTCGAGB 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 131
RsaI GTAC 1 cut(s) 781
RsaNI GTAC 1 cut(s) 780
SacI GAGCTC 2 cut(s) 515, 521
SaqAI TTAA 5 cut(s) 297, 645, 654, 711, 812
SatI GCNGC 2 cut(s) 120, 285
Sau96I GGNCC 2 cut(s) 72, 308
SchI GAGTC 1 cut(s) 605
ScrFI CCNGG 5 cut(s) 67, 233, 312, 416, 447
SduI GDGCHC 4 cut(s) 414, 424, 515, 521
SfaNI GCATC 2 cut(s) 51, 250
SfcI CTRYAG 1 cut(s) 767
Sfr274I CTCGAG 1 cut(s) 514
SinI GGWCC 1 cut(s) 72
SlaI CTCGAG 1 cut(s) 514
SmlI CTYRAG 3 cut(s) 296, 514, 628
SmoI CTYRAG 3 cut(s) 296, 514, 628
Sse9I AATT 3 cut(s) 123, 279, 731
SsiI CCGC 2 cut(s) 604, 633
SspMI CTAG 2 cut(s) 266, 474
SstI GAGCTC 2 cut(s) 515, 521
StyD4I CCNGG 5 cut(s) 65, 231, 310, 414, 445
StyI CCWWGG 2 cut(s) 255, 304
TaaI ACNGT 2 cut(s) 621, 832
TaqI TCGA 1 cut(s) 515
TasI AATT 3 cut(s) 123, 279, 731
TfiI GAWTC 4 cut(s) 105, 169, 187, 501
Tru1I TTAA 5 cut(s) 297, 645, 654, 711, 812
Tru9I TTAA 5 cut(s) 297, 645, 654, 711, 812
TscAI CASTG 1 cut(s) 711
TseFI GTSAC 1 cut(s) 314
TseI GCWGC 2 cut(s) 119, 284
Tsp45I GTSAC 1 cut(s) 314
TspDTI ATGAA 5 cut(s) 182, 376, 731, 830, 858
TspRI CASTG 1 cut(s) 711
Van91I CCANNNNNTGG 1 cut(s) 82
Vha464I CTTAAG 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 72
XagI CCTNNNNNAGG 1 cut(s) 843
XceI RCATGY 2 cut(s) 114, 780
XcmI CCANNNNNNNNNTGG 1 cut(s) 236
XhoI CTCGAG 1 cut(s) 514
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 2 cut(s) 266, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.