RchiOBHm_Chr1g0337231

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002561
Physical Location & Seq
Reverse (-)
28909950 .. 28910360
411 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56514

Sequence Viewer

Length: 411 bp
ATGTCATCGAGTTTCAACAGTATGGAAGAGGTACAGAAGGTCTTCAACAAGTTCGATGAGAACAGTGACGGACTCATCTCCAGTGATGAGCTCAATAACGTTCTCCGTAAGATGGGGCGGGAGATGAAGCCAGAGGAGTTCGACAAGGACTGGGACGGCCACATCGACCTGGAGGAGTTCACAAAAATCATGAATGGATCGGGGTCGAGCAAGGATCTCCTTGACGCCTTCAATGTGTACGATCTTGACAAGGATGGCCTGATCTCGGTGAGCGAACTTCATGAGGTGCTCAACCGCTTAGGGGAGAAGTGCTCCGTTGGGGAATGTGCCACCATGATCACCAAATTTGACGTCAATGGTGATGGCTTCATCAACTTTGAAGAGTTCAAGCAGATGATGAACCGCTCTTGA

Protein Analysis

136

Amino Acids

15.51

Weight (kDa)

4.42

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 9 - 64 1.7e-08 EF-hand domain pair
EF-hand_1 PF00036 10 - 38 2.4e-06 EF hand domain
EF-hand_6 PF13405 11 - 39 1.5e-06 EF-hand domain
EF-hand_5 PF13202 12 - 35 1e-05 EF hand
EF-hand_7 PF13499 71 - 133 3.4e-15 EF-hand domain pair
EF-hand_1 PF00036 73 - 99 4.6e-06 EF hand domain
EF-hand_6 PF13405 73 - 101 1.4e-06 EF-hand domain
EF-hand_5 PF13202 75 - 96 6e-06 EF hand
EF-hand_8 PF13833 100 - 135 5.1e-07 EF-hand domain pair
EF-hand_1 PF00036 109 - 135 3.4e-09 EF hand domain
EF-hand_5 PF13202 115 - 133 9.8e-07 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 354
AccBSI CCGCTC 1 cut(s) 405
AciI CCGC 3 cut(s) 118, 295, 403
AclI AACGTT 1 cut(s) 99
AclWI GGATC 2 cut(s) 205, 222
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 1 cut(s) 344
AcyI GRCGYC 2 cut(s) 225, 351
AfaI GTAC 2 cut(s) 33, 239
AfiI CCNNNNNNNGG 3 cut(s) 112, 265, 301
AgsI TTSAA 5 cut(s) 16, 46, 232, 380, 388
AjnI CCWGG 1 cut(s) 168
AluBI AGCT 1 cut(s) 91
AluI AGCT 1 cut(s) 91
Alw21I GWGCWC 3 cut(s) 93, 291, 314
AlwI GGATC 2 cut(s) 205, 222
AoxI GGCC 2 cut(s) 157, 256
ApoI RAATTY 1 cut(s) 344
ArsI GACNNNNNNTTYG 2 cut(s) 336, 368
Asp700I GAANNNNTTC 1 cut(s) 41
AsuHPI GGTGA 3 cut(s) 280, 331, 371
BanII GRGCYC 1 cut(s) 93
BbsI GAAGAC 1 cut(s) 34
Bbv12I GWGCWC 3 cut(s) 93, 291, 314
BccI CCATC 3 cut(s) 106, 248, 356
BceAI ACGGC 1 cut(s) 172
BciT130I CCWGG 1 cut(s) 170
BclI TGATCA 1 cut(s) 336
Bme1390I CCNGG 1 cut(s) 170
BmrFI CCNGG 1 cut(s) 170
BmrI ACTGGG 1 cut(s) 160
BmuI ACTGGG 1 cut(s) 160
BpiI GAAGAC 1 cut(s) 34
BplI GAGNNNNNCTC 2 cut(s) 296, 328
BpmI CTGGAG 2 cut(s) 64, 191
Bpu10I CCTNAGC 1 cut(s) 298
BsaHI GRCGYC 2 cut(s) 225, 351
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 265, 301
Bse1I ACTGG 2 cut(s) 81, 155
BseBI CCWGG 1 cut(s) 170
BseGI GGATG 1 cut(s) 259
BseLI CCNNNNNNNGG 3 cut(s) 112, 265, 301
BseNI ACTGG 2 cut(s) 81, 155
BseRI GAGGAG 2 cut(s) 149, 188
BshFI GGCC 2 cut(s) 159, 258
BsiHKAI GWGCWC 3 cut(s) 93, 291, 314
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 3 cut(s) 112, 265, 301
BsmFI GGGAC 1 cut(s) 167
BsnI GGCC 2 cut(s) 159, 258
Bsp1286I GDGCHC 3 cut(s) 93, 291, 314
Bsp143I GATC 5 cut(s) 197, 214, 241, 261, 336
BspACI CCGC 3 cut(s) 118, 295, 403
BspANI GGCC 2 cut(s) 159, 258
BspHI TCATGA 2 cut(s) 189, 280
BspPI GGATC 2 cut(s) 205, 222
BsrBI CCGCTC 1 cut(s) 405
BsrI ACTGG 2 cut(s) 81, 155
BssMI GATC 5 cut(s) 197, 214, 241, 261, 336
BssNI GRCGYC 2 cut(s) 225, 351
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 20, 65
Bst6I CTCTTC 2 cut(s) 21, 375
BstACI GRCGYC 2 cut(s) 225, 351
BstDEI CTNAG 1 cut(s) 298
BstF5I GGATG 1 cut(s) 259
BstKTI GATC 5 cut(s) 200, 217, 244, 264, 339
BstMBI GATC 5 cut(s) 197, 214, 241, 261, 336
BstNI CCWGG 1 cut(s) 170
BstSCI CCNGG 1 cut(s) 168
BstV2I GAAGAC 1 cut(s) 34
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
BsuRI GGCC 2 cut(s) 159, 258
BtsCI GGATG 1 cut(s) 259
BtsIMutI CAGTG 2 cut(s) 70, 88
CciI TCATGA 2 cut(s) 189, 280
CseI GACGC 1 cut(s) 233
Csp6I GTAC 2 cut(s) 32, 238
CviAII CATG 3 cut(s) 190, 281, 334
CviJI RGCY 5 cut(s) 91, 130, 159, 258, 366
CviKI_1 RGCY 5 cut(s) 91, 130, 159, 258, 366
CviQI GTAC 2 cut(s) 32, 238
DdeI CTNAG 1 cut(s) 298
DpnI GATC 5 cut(s) 199, 216, 243, 263, 338
DpnII GATC 5 cut(s) 197, 214, 241, 261, 336
EaeI YGGCCR 1 cut(s) 157
Eam1104I CTCTTC 2 cut(s) 21, 375
EarI CTCTTC 2 cut(s) 21, 375
Ecl136II GAGCTC 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 93
Eco53kI GAGCTC 1 cut(s) 91
EcoICRI GAGCTC 1 cut(s) 91
EcoRII CCWGG 1 cut(s) 168
EcoT38I GRGCYC 1 cut(s) 93
FaeI CATG 3 cut(s) 193, 284, 337
FaiI YATR 4 cut(s) 23, 191, 282, 335
FaqI GGGAC 1 cut(s) 167
FatI CATG 3 cut(s) 189, 280, 333
FauI CCCGC 1 cut(s) 111
FbaI TGATCA 1 cut(s) 336
FokI GGATG 1 cut(s) 266
FriOI GRGCYC 1 cut(s) 93
GsuI CTGGAG 2 cut(s) 64, 191
HaeIII GGCC 2 cut(s) 159, 258
HgaI GACGC 1 cut(s) 233
Hin1I GRCGYC 2 cut(s) 225, 351
Hin1II CATG 3 cut(s) 193, 284, 337
HinfI GANTC 1 cut(s) 72
HphI GGTGA 3 cut(s) 280, 331, 371
Hpy166II GTNNAC 2 cut(s) 180, 238
Hpy188III TCNNGA 4 cut(s) 190, 245, 281, 408
Hpy8I GTNNAC 2 cut(s) 180, 238
HpyAV CCTTC 2 cut(s) 31, 238
HpyCH4III ACNGT 2 cut(s) 20, 65
HpyCH4IV ACGT 2 cut(s) 99, 351
HpyF3I CTNAG 1 cut(s) 298
HpySE526I ACGT 2 cut(s) 99, 351
Hsp92I GRCGYC 2 cut(s) 225, 351
Hsp92II CATG 3 cut(s) 193, 284, 337
Ksp22I TGATCA 1 cut(s) 336
Kzo9I GATC 5 cut(s) 197, 214, 241, 261, 336
LmnI GCTCC 1 cut(s) 317
LpnPI CCDG 6 cut(s) 94, 136, 144, 155, 182, 272
MaeII ACGT 2 cut(s) 99, 351
MaeIII GTNAC 1 cut(s) 65
MalI GATC 5 cut(s) 199, 216, 243, 263, 338
MbiI CCGCTC 1 cut(s) 405
MboI GATC 5 cut(s) 197, 214, 241, 261, 336
MboII GAAGA 3 cut(s) 34, 38, 392
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 3 cut(s) 93, 291, 314
MluCI AATT 1 cut(s) 344
MlyI GAGTC 1 cut(s) 66
MnlI CCTC 4 cut(s) 22, 127, 166, 277
MroXI GAANNNNTTC 1 cut(s) 41
MspR9I CCNGG 1 cut(s) 170
MvaI CCWGG 1 cut(s) 170
NdeII GATC 5 cut(s) 197, 214, 241, 261, 336
NlaIII CATG 3 cut(s) 193, 284, 337
NmuCI GTSAC 1 cut(s) 65
PagI TCATGA 2 cut(s) 189, 280
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PdmI GAANNNNTTC 1 cut(s) 41
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
Psp124BI GAGCTC 1 cut(s) 93
Psp1406I AACGTT 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PsuI RGATCY 1 cut(s) 214
RsaI GTAC 2 cut(s) 33, 239
RsaNI GTAC 2 cut(s) 32, 238
SacI GAGCTC 1 cut(s) 93
Sau3AI GATC 5 cut(s) 197, 214, 241, 261, 336
SchI GAGTC 1 cut(s) 66
ScrFI CCNGG 1 cut(s) 170
SduI GDGCHC 3 cut(s) 93, 291, 314
SetI ASST 7 cut(s) 33, 42, 93, 102, 171, 288, 354
Sse9I AATT 1 cut(s) 344
SsiI CCGC 3 cut(s) 118, 295, 403
SstI GAGCTC 1 cut(s) 93
StyD4I CCNGG 1 cut(s) 168
TaaI ACNGT 2 cut(s) 20, 65
TaiI ACGT 2 cut(s) 102, 354
TaqI TCGA 5 cut(s) 8, 54, 141, 165, 206
TasI AATT 1 cut(s) 344
TscAI CASTG 2 cut(s) 70, 88
TseFI GTSAC 1 cut(s) 65
Tsp45I GTSAC 1 cut(s) 65
TspDTI ATGAA 4 cut(s) 140, 206, 269, 358
TspGWI ACGGA 3 cut(s) 84, 95, 304
TspRI CASTG 2 cut(s) 70, 88
XapI RAATTY 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 41
ZraI GACGTC 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.