Rh1DG160500

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0002561
Physical Location & Seq
Reverse (-)
33070935 .. 33087119
16185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG160500.1

Sequence Viewer

Length: 690 bp
ATGGTATGCATGTGGGTCAAAGAAACACTTAAAAATGGGAAGACCATTGTCTGCAGCTTTGATGAGAAGATTTTCGGACATCCGTTCAAAACTTTTGTTACTAAGAAGGATGTTTATAACTTTGCCACTATGAATGAAGTATCGGGTAGCTGCGAACACTGGGATATCTACCCTCTCACACAATCCAATCCTAACAAGCAAGAAGAAACCAAATCAAATTTGTACTCCCTCTCAGCGATCTCATCGAGTTTCAACAGTTCAAAAAGCGGCAGCAGCGTAGTGGTACCTATGGAAGAGGTACAGAAGGTCTTCAACAAGTTCGATGAGAACAGTGACGGACTCATCTCCAGTGATGAGCTCAATAACGTTCTCCGTAAGATGGGGCAGGAGATGAAGCCAGAGGAGTTCGACAAGGACTGGGACGGCCACATCGACCTGGAGGAGTTCATAAAAATCATGAATGGATCGGGGTCGAGCAAGGATCTCCTTGACGCCTTACGTGACGCCTTCAATGTGTACGATCTCGACAAGAATGGCCTGATCTCGGCGAGCGAACTTCATGAGGTGCTCAACCGCTTAGGGGAGAAGTGCTCCGTTGGGGAATGTGCCACCATGATCACCAAATTTGACGTCAATGGTGATGGCTTCATCAACTTTGAAGAGTTCAAGCAGATGATGAACCGCTCTTGA

Protein Analysis

229

Amino Acids

25.93

Weight (kDa)

4.79

Isoelectric Point (pI)

36.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 98 - 153 1e-08 EF-hand domain pair
EF-hand_1 PF00036 99 - 127 5.4e-06 EF hand domain
EF-hand_6 PF13405 100 - 128 3.6e-06 EF-hand domain
EF-hand_7 PF13499 164 - 226 9.9e-15 EF-hand domain pair
EF-hand_1 PF00036 166 - 192 1e-05 EF hand domain
EF-hand_6 PF13405 166 - 194 1.2e-06 EF-hand domain
EF-hand_8 PF13833 192 - 228 1.1e-06 EF-hand domain pair
EF-hand_1 PF00036 202 - 228 7.7e-09 EF hand domain
EF-hand_5 PF13202 208 - 226 2.1e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 117
AatII GACGTC 1 cut(s) 633
Acc65I GGTACC 1 cut(s) 283
AccB1I GGYRCC 1 cut(s) 283
AccBSI CCGCTC 1 cut(s) 684
AciI CCGC 3 cut(s) 267, 574, 682
AclI AACGTT 1 cut(s) 366
AclWI GGATC 2 cut(s) 472, 489
AcoI YGGCCR 1 cut(s) 424
AcsI RAATTY 2 cut(s) 217, 623
AcyI GRCGYC 3 cut(s) 492, 504, 630
AfaI GTAC 4 cut(s) 224, 285, 300, 518
AfiI CCNNNNNNNGG 3 cut(s) 379, 544, 580
AgsI TTSAA 7 cut(s) 88, 253, 261, 313, 511, 659, 667
AjnI CCWGG 1 cut(s) 435
AluBI AGCT 3 cut(s) 57, 150, 358
AluI AGCT 3 cut(s) 57, 150, 358
Alw21I GWGCWC 3 cut(s) 360, 570, 593
AlwI GGATC 2 cut(s) 472, 489
AoxI GGCC 2 cut(s) 424, 535
ApeKI GCWGC 4 cut(s) 54, 150, 270, 273
ApoI RAATTY 2 cut(s) 217, 623
ArsI GACNNNNNNTTYG 2 cut(s) 615, 647
Asp700I GAANNNNTTC 2 cut(s) 71, 308
Asp718I GGTACC 1 cut(s) 283
AsuHPI GGTGA 2 cut(s) 610, 650
BanI GGYRCC 1 cut(s) 283
BanII GRGCYC 1 cut(s) 360
BbsI GAAGAC 2 cut(s) 47, 301
Bbv12I GWGCWC 3 cut(s) 360, 570, 593
BbvI GCAGC 4 cut(s) 66, 137, 282, 285
BccI CCATC 2 cut(s) 373, 635
BceAI ACGGC 1 cut(s) 439
BciT130I CCWGG 1 cut(s) 437
BclI TGATCA 1 cut(s) 615
BfmI CTRYAG 1 cut(s) 52
BisI GCNGC 5 cut(s) 55, 151, 268, 271, 274
BlsI GCNGC 5 cut(s) 56, 152, 269, 272, 275
Bme1390I CCNGG 1 cut(s) 437
BmiI GGNNCC 1 cut(s) 285
BmrFI CCNGG 1 cut(s) 437
BmrI ACTGGG 2 cut(s) 169, 427
BmuI ACTGGG 2 cut(s) 169, 427
BoxI GACNNNNGTC 1 cut(s) 47
BpiI GAAGAC 2 cut(s) 47, 301
BplI GAGNNNNNCTC 2 cut(s) 575, 607
BpmI CTGGAG 2 cut(s) 331, 458
Bpu10I CCTNAGC 1 cut(s) 577
BsaAI YACGTR 1 cut(s) 500
BsaHI GRCGYC 3 cut(s) 492, 504, 630
Bsc4I CCNNNNNNNGG 3 cut(s) 379, 544, 580
Bse1I ACTGG 3 cut(s) 164, 348, 422
BseBI CCWGG 1 cut(s) 437
BseGI GGATG 2 cut(s) 79, 115
BseLI CCNNNNNNNGG 3 cut(s) 379, 544, 580
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 3 cut(s) 164, 348, 422
BseRI GAGGAG 2 cut(s) 416, 455
BseXI GCAGC 4 cut(s) 66, 137, 282, 285
BshFI GGCC 2 cut(s) 426, 537
BshNI GGYRCC 1 cut(s) 283
BsiHKAI GWGCWC 3 cut(s) 360, 570, 593
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 3 cut(s) 379, 544, 580
BsmFI GGGAC 1 cut(s) 434
BsnI GGCC 2 cut(s) 426, 537
Bsp1286I GDGCHC 3 cut(s) 360, 570, 593
Bsp143I GATC 6 cut(s) 237, 464, 481, 520, 540, 615
BspACI CCGC 3 cut(s) 267, 574, 682
BspANI GGCC 2 cut(s) 426, 537
BspCNI CTCAG 1 cut(s) 245
BspHI TCATGA 2 cut(s) 456, 559
BspLI GGNNCC 1 cut(s) 285
BspMAI CTGCAG 1 cut(s) 56
BspPI GGATC 2 cut(s) 472, 489
BspT107I GGYRCC 1 cut(s) 283
BsrBI CCGCTC 1 cut(s) 684
BsrI ACTGG 3 cut(s) 164, 348, 422
BssMI GATC 6 cut(s) 237, 464, 481, 520, 540, 615
BssNI GRCGYC 3 cut(s) 492, 504, 630
Bst2UI CCWGG 1 cut(s) 437
Bst4CI ACNGT 2 cut(s) 257, 332
Bst6I CTCTTC 2 cut(s) 288, 654
BstACI GRCGYC 3 cut(s) 492, 504, 630
BstBAI YACGTR 1 cut(s) 500
BstC8I GCNNGC 1 cut(s) 550
BstDEI CTNAG 3 cut(s) 102, 232, 577
BstF5I GGATG 2 cut(s) 79, 115
BstKTI GATC 6 cut(s) 240, 467, 484, 523, 543, 618
BstMBI GATC 6 cut(s) 237, 464, 481, 520, 540, 615
BstMWI GCNNNNNNNGC 1 cut(s) 273
BstNI CCWGG 1 cut(s) 437
BstNSI RCATGY 1 cut(s) 13
BstPAI GACNNNNGTC 1 cut(s) 47
BstSCI CCNGG 1 cut(s) 435
BstSFI CTRYAG 1 cut(s) 52
BstV1I GCAGC 4 cut(s) 66, 137, 282, 285
BstV2I GAAGAC 2 cut(s) 47, 301
BstX2I RGATCY 1 cut(s) 481
BstYI RGATCY 1 cut(s) 481
BsuRI GGCC 2 cut(s) 426, 537
BtsCI GGATG 2 cut(s) 79, 115
BtsIMutI CAGTG 3 cut(s) 157, 337, 355
Cac8I GCNNGC 1 cut(s) 550
CciI TCATGA 2 cut(s) 456, 559
CseI GACGC 2 cut(s) 500, 512
Csp6I GTAC 4 cut(s) 223, 284, 299, 517
CviAII CATG 4 cut(s) 10, 457, 560, 613
CviJI RGCY 7 cut(s) 57, 150, 358, 397, 426, 537, 645
CviKI_1 RGCY 7 cut(s) 57, 150, 358, 397, 426, 537, 645
CviQI GTAC 4 cut(s) 223, 284, 299, 517
DdeI CTNAG 3 cut(s) 102, 232, 577
DpnI GATC 6 cut(s) 239, 466, 483, 522, 542, 617
DpnII GATC 6 cut(s) 237, 464, 481, 520, 540, 615
EaeI YGGCCR 1 cut(s) 424
Eam1104I CTCTTC 2 cut(s) 288, 654
EarI CTCTTC 2 cut(s) 288, 654
Ecl136II GAGCTC 1 cut(s) 358
Eco24I GRGCYC 1 cut(s) 360
Eco32I GATATC 1 cut(s) 166
Eco53kI GAGCTC 1 cut(s) 358
EcoICRI GAGCTC 1 cut(s) 358
EcoRII CCWGG 1 cut(s) 435
EcoRV GATATC 1 cut(s) 166
EcoT22I ATGCAT 1 cut(s) 11
EcoT38I GRGCYC 1 cut(s) 360
FaeI CATG 4 cut(s) 13, 460, 563, 616
FaiI YATR 9 cut(s) 7, 11, 117, 131, 290, 449, 458, 561, 614
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FaqI GGGAC 1 cut(s) 434
FatI CATG 4 cut(s) 9, 456, 559, 612
FbaI TGATCA 1 cut(s) 615
Fnu4HI GCNGC 5 cut(s) 55, 151, 268, 271, 274
FokI GGATG 2 cut(s) 66, 122
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 5 cut(s) 55, 151, 268, 271, 274
GluI GCNGC 5 cut(s) 55, 151, 268, 271, 274
GsuI CTGGAG 2 cut(s) 331, 458
HaeIII GGCC 2 cut(s) 426, 537
HgaI GACGC 2 cut(s) 500, 512
Hin1I GRCGYC 3 cut(s) 492, 504, 630
Hin1II CATG 4 cut(s) 13, 460, 563, 616
HinfI GANTC 1 cut(s) 339
HphI GGTGA 2 cut(s) 610, 650
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 1 cut(s) 77
Hpy188III TCNNGA 4 cut(s) 457, 524, 560, 687
Hpy8I GTNNAC 1 cut(s) 517
HpyAV CCTTC 3 cut(s) 100, 298, 517
HpyCH4III ACNGT 2 cut(s) 257, 332
HpyCH4IV ACGT 3 cut(s) 366, 499, 630
HpyCH4V TGCA 2 cut(s) 9, 54
HpyF10VI GCNNNNNNNGC 1 cut(s) 273
HpyF3I CTNAG 3 cut(s) 102, 232, 577
HpySE526I ACGT 3 cut(s) 366, 499, 630
Hsp92I GRCGYC 3 cut(s) 492, 504, 630
Hsp92II CATG 4 cut(s) 13, 460, 563, 616
KpnI GGTACC 1 cut(s) 287
Ksp22I TGATCA 1 cut(s) 615
Kzo9I GATC 6 cut(s) 237, 464, 481, 520, 540, 615
LmnI GCTCC 1 cut(s) 596
LpnPI CCDG 8 cut(s) 145, 361, 371, 403, 411, 422, 449, 551
Lsp1109I GCAGC 4 cut(s) 66, 137, 282, 285
MaeII ACGT 3 cut(s) 366, 499, 630
MaeIII GTNAC 3 cut(s) 97, 332, 500
MalI GATC 6 cut(s) 239, 466, 483, 522, 542, 617
MbiI CCGCTC 1 cut(s) 684
MboI GATC 6 cut(s) 237, 464, 481, 520, 540, 615
MboII GAAGA 6 cut(s) 52, 79, 215, 301, 305, 671
MflI RGATCY 1 cut(s) 481
MhlI GDGCHC 3 cut(s) 360, 570, 593
MluCI AATT 2 cut(s) 217, 623
MlyI GAGTC 1 cut(s) 333
MnlI CCTC 6 cut(s) 183, 239, 289, 394, 433, 556
Mph1103I ATGCAT 1 cut(s) 11
MroXI GAANNNNTTC 2 cut(s) 71, 308
MseI TTAA 1 cut(s) 30
MspR9I CCNGG 1 cut(s) 437
MvaI CCWGG 1 cut(s) 437
MwoI GCNNNNNNNGC 1 cut(s) 273
NdeII GATC 6 cut(s) 237, 464, 481, 520, 540, 615
NlaIII CATG 4 cut(s) 13, 460, 563, 616
NlaIV GGNNCC 1 cut(s) 285
NmeAIII GCCGAG 1 cut(s) 524
NmuCI GTSAC 2 cut(s) 332, 500
NsiI ATGCAT 1 cut(s) 11
NspI RCATGY 1 cut(s) 13
PagI TCATGA 2 cut(s) 456, 559
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PdmI GAANNNNTTC 2 cut(s) 71, 308
PkrI GCNGC 5 cut(s) 56, 152, 269, 272, 275
PleI GAGTC 1 cut(s) 333
PpsI GAGTC 1 cut(s) 333
Ppu21I YACGTR 1 cut(s) 500
PshAI GACNNNNGTC 1 cut(s) 47
PsiI TTATAA 1 cut(s) 117
Psp124BI GAGCTC 1 cut(s) 360
Psp1406I AACGTT 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 435
PspGI CCWGG 1 cut(s) 435
PspN4I GGNNCC 1 cut(s) 285
PstI CTGCAG 1 cut(s) 56
PsuI RGATCY 1 cut(s) 481
RsaI GTAC 4 cut(s) 224, 285, 300, 518
RsaNI GTAC 4 cut(s) 223, 284, 299, 517
SacI GAGCTC 1 cut(s) 360
SaqAI TTAA 1 cut(s) 30
SatI GCNGC 5 cut(s) 55, 151, 268, 271, 274
Sau3AI GATC 6 cut(s) 237, 464, 481, 520, 540, 615
SchI GAGTC 1 cut(s) 333
ScrFI CCNGG 1 cut(s) 437
SduI GDGCHC 3 cut(s) 360, 570, 593
SfcI CTRYAG 1 cut(s) 52
Sse9I AATT 2 cut(s) 217, 623
SsiI CCGC 3 cut(s) 267, 574, 682
SstI GAGCTC 1 cut(s) 360
StyD4I CCNGG 1 cut(s) 435
TaaI ACNGT 2 cut(s) 257, 332
TaiI ACGT 3 cut(s) 369, 502, 633
TaqI TCGA 6 cut(s) 245, 321, 408, 432, 473, 525
TasI AATT 2 cut(s) 217, 623
TatI WGTACW 1 cut(s) 222
TauI GCSGC 1 cut(s) 270
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TscAI CASTG 3 cut(s) 164, 337, 355
TseFI GTSAC 2 cut(s) 332, 500
TseI GCWGC 4 cut(s) 54, 150, 270, 273
Tsp45I GTSAC 2 cut(s) 332, 500
TspDTI ATGAA 7 cut(s) 146, 150, 407, 436, 473, 548, 637
TspGWI ACGGA 4 cut(s) 72, 351, 362, 583
TspRI CASTG 3 cut(s) 164, 337, 355
XapI RAATTY 2 cut(s) 217, 623
XceI RCATGY 1 cut(s) 13
XmnI GAANNNNTTC 2 cut(s) 71, 308
ZraI GACGTC 1 cut(s) 631
Zsp2I ATGCAT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.