RLG00000029299

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002561
Physical Location & Seq
Forward (+)
36207079 .. 36207540
462 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029299

Sequence Viewer

Length: 462 bp
ATGTCATCGAGTTTCAACAGTTCAAAAAGCGGCAGCGGCGTGGTGGTACCTATGGAAGAGGTACAGAAGGTCTTCGACAAGTTCGACGAGAACAGTGACGGACTCATCTCCAGTGATGAGATCAAAAACGTTCTCCGTGGGATGGGGTGCGAGATGGAGCCAAAGGAATTGCAGACTCTGATGTCCGAGTTCGACAAGGATGGAGACGGCCACATCAACCTGGAGGAGTTCACAGAAATCATGAACAGATCGGGGTCGAGCAAGGATCTCCGTGATGCCTTCGATGTCTTCGATCGCGACAAGGACGGCCTGATCTCGGCGAGCGAACTTCATGAGGCCCTCAACCGCTTAGGGATGAAGTGCTCTGTAGGGGAATGTGCCACCATGATCACTAAATTTGACATCAATGGCGATGGTTGCGTTAACTTTCACGAGTTCAAGAAGATGATGAACTTCTCTTAA

Protein Analysis

154

Amino Acids

17.05

Weight (kDa)

4.56

Isoelectric Point (pI)

45.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 19 - 81 4.9e-17 EF-hand domain pair
EF-hand_1 PF00036 20 - 47 7.5e-06 EF hand domain
EF-hand_6 PF13405 21 - 49 1.5e-06 EF-hand domain
EF-hand_9 PF14658 24 - 83 7.6e-07 EF-hand domain
EF-hand_8 PF13833 52 - 83 1.1e-07 EF-hand domain pair
EF-hand_1 PF00036 56 - 83 1.9e-08 EF hand domain
EF-hand_7 PF13499 88 - 150 7.2e-15 EF-hand domain pair
EF-hand_1 PF00036 89 - 116 4.4e-07 EF hand domain
EF-hand_6 PF13405 89 - 118 4.3e-08 EF-hand domain
EF-hand_5 PF13202 90 - 111 1.8e-06 EF hand
EF-hand_8 PF13833 110 - 150 4.1e-07 EF-hand domain pair
EF-hand_1 PF00036 126 - 151 8.9e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 181
Acc65I GGTACC 1 cut(s) 46
AccB1I GGYRCC 1 cut(s) 46
AccII CGCG 1 cut(s) 297
AciI CCGC 3 cut(s) 30, 36, 346
AclI AACGTT 1 cut(s) 129
AclWI GGATC 1 cut(s) 273
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 1 cut(s) 395
AfaI GTAC 2 cut(s) 48, 63
AfiI CCNNNNNNNGG 2 cut(s) 142, 316
AgsI TTSAA 3 cut(s) 16, 24, 439
AjnI CCWGG 1 cut(s) 219
Alw21I GWGCWC 1 cut(s) 365
Alw26I GTCTC 1 cut(s) 198
AlwI GGATC 1 cut(s) 273
AlwNI CAGNNNCTG 1 cut(s) 178
AoxI GGCC 3 cut(s) 208, 307, 336
ApeKI GCWGC 1 cut(s) 33
ApoI RAATTY 1 cut(s) 395
Asp700I GAANNNNTTC 1 cut(s) 71
Asp718I GGTACC 1 cut(s) 46
AspS9I GGNCC 1 cut(s) 337
BanI GGYRCC 1 cut(s) 46
BauI CACGAG 1 cut(s) 431
BbsI GAAGAC 2 cut(s) 64, 280
Bbv12I GWGCWC 1 cut(s) 365
BbvI GCAGC 1 cut(s) 45
BccI CCATC 4 cut(s) 136, 148, 194, 407
BceAI ACGGC 2 cut(s) 223, 322
BciT130I CCWGG 1 cut(s) 221
BclI TGATCA 1 cut(s) 387
BcoDI GTCTC 1 cut(s) 198
BfmI CTRYAG 1 cut(s) 366
BisI GCNGC 3 cut(s) 31, 34, 37
BlsI GCNGC 3 cut(s) 32, 35, 38
Bme1390I CCNGG 1 cut(s) 221
BmgT120I GGNCC 1 cut(s) 337
BmiI GGNNCC 2 cut(s) 48, 159
BmrFI CCNGG 1 cut(s) 221
BmsI GCATC 1 cut(s) 265
BpiI GAAGAC 2 cut(s) 64, 280
BpmI CTGGAG 2 cut(s) 94, 242
Bpu10I CCTNAGC 1 cut(s) 349
BsaJI CCNNGG 1 cut(s) 136
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 316
Bse1I ACTGG 1 cut(s) 111
BseBI CCWGG 1 cut(s) 221
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 3 cut(s) 147, 205, 360
BseLI CCNNNNNNNGG 2 cut(s) 142, 316
BseNI ACTGG 1 cut(s) 111
BseRI GAGGAG 1 cut(s) 239
BseXI GCAGC 1 cut(s) 45
Bsh1236I CGCG 1 cut(s) 297
Bsh1285I CGRYCG 1 cut(s) 295
BshFI GGCC 3 cut(s) 210, 309, 338
BshNI GGYRCC 1 cut(s) 46
BsiEI CGRYCG 1 cut(s) 295
BsiHKAI GWGCWC 1 cut(s) 365
BslI CCNNNNNNNGG 2 cut(s) 142, 316
BsmAI GTCTC 1 cut(s) 198
BsmBI CGTCTC 1 cut(s) 198
BsnI GGCC 3 cut(s) 210, 309, 338
Bsp1286I GDGCHC 1 cut(s) 365
Bsp143I GATC 6 cut(s) 120, 248, 265, 292, 312, 387
Bsp68I TCGCGA 1 cut(s) 297
BspACI CCGC 3 cut(s) 30, 36, 346
BspANI GGCC 3 cut(s) 210, 309, 338
BspFNI CGCG 1 cut(s) 297
BspHI TCATGA 2 cut(s) 240, 331
BspLI GGNNCC 2 cut(s) 48, 159
BspPI GGATC 1 cut(s) 273
BspT107I GGYRCC 1 cut(s) 46
BsrI ACTGG 1 cut(s) 111
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 6 cut(s) 120, 248, 265, 292, 312, 387
BssSI CACGAG 1 cut(s) 431
Bst2BI CACGAG 1 cut(s) 431
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 2 cut(s) 20, 95
Bst6I CTCTTC 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 322
BstDEI CTNAG 1 cut(s) 349
BstDSI CCRYGG 1 cut(s) 136
BstF5I GGATG 3 cut(s) 147, 205, 360
BstFNI CGCG 1 cut(s) 297
BstKTI GATC 6 cut(s) 123, 251, 268, 295, 315, 390
BstMAI GTCTC 1 cut(s) 198
BstMBI GATC 6 cut(s) 120, 248, 265, 292, 312, 387
BstMCI CGRYCG 1 cut(s) 295
BstMWI GCNNNNNNNGC 2 cut(s) 36, 417
BstNI CCWGG 1 cut(s) 221
BstSCI CCNGG 1 cut(s) 219
BstSFI CTRYAG 1 cut(s) 366
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 1 cut(s) 45
BstV2I GAAGAC 2 cut(s) 64, 280
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BsuRI GGCC 3 cut(s) 210, 309, 338
BtgI CCRYGG 1 cut(s) 136
BtgZI GCGATG 1 cut(s) 426
BtsCI GGATG 3 cut(s) 147, 205, 360
BtsIMutI CAGTG 2 cut(s) 100, 118
BtuMI TCGCGA 1 cut(s) 297
Cac8I GCNNGC 1 cut(s) 322
CaiI CAGNNNCTG 1 cut(s) 178
CciI TCATGA 2 cut(s) 240, 331
Cfr13I GGNCC 1 cut(s) 337
Csp6I GTAC 2 cut(s) 47, 62
CviAII CATG 3 cut(s) 241, 332, 385
CviJI RGCY 4 cut(s) 160, 210, 309, 338
CviKI_1 RGCY 4 cut(s) 160, 210, 309, 338
CviQI GTAC 2 cut(s) 47, 62
DdeI CTNAG 1 cut(s) 349
DpnI GATC 6 cut(s) 122, 250, 267, 294, 314, 389
DpnII GATC 6 cut(s) 120, 248, 265, 292, 312, 387
DrdI GACNNNNNNGTC 1 cut(s) 181
DseDI GACNNNNNNGTC 1 cut(s) 181
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
EcoO109I RGGNCCY 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 219
Esp3I CGTCTC 1 cut(s) 198
FaeI CATG 3 cut(s) 244, 335, 388
FaiI YATR 4 cut(s) 53, 242, 333, 386
FatI CATG 3 cut(s) 240, 331, 384
FbaI TGATCA 1 cut(s) 387
Fnu4HI GCNGC 3 cut(s) 31, 34, 37
FokI GGATG 3 cut(s) 154, 212, 367
Fsp4HI GCNGC 3 cut(s) 31, 34, 37
GluI GCNGC 3 cut(s) 31, 34, 37
GsuI CTGGAG 2 cut(s) 94, 242
HaeIII GGCC 3 cut(s) 210, 309, 338
Hin1II CATG 3 cut(s) 244, 335, 388
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 2 cut(s) 102, 175
HpaI GTTAAC 1 cut(s) 424
Hpy166II GTNNAC 2 cut(s) 231, 424
Hpy188I TCNGA 2 cut(s) 180, 187
Hpy188III TCNNGA 5 cut(s) 241, 296, 332, 431, 439
Hpy8I GTNNAC 2 cut(s) 231, 424
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 2 cut(s) 61, 289
HpyCH4III ACNGT 2 cut(s) 20, 95
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 1 cut(s) 172
HpyF10VI GCNNNNNNNGC 2 cut(s) 36, 417
HpyF3I CTNAG 1 cut(s) 349
HpySE526I ACGT 1 cut(s) 129
Hsp92II CATG 3 cut(s) 244, 335, 388
KpnI GGTACC 1 cut(s) 50
Ksp22I TGATCA 1 cut(s) 387
KspAI GTTAAC 1 cut(s) 424
Kzo9I GATC 6 cut(s) 120, 248, 265, 292, 312, 387
LmnI GCTCC 1 cut(s) 157
LpnPI CCDG 4 cut(s) 124, 206, 233, 323
Lsp1109I GCAGC 1 cut(s) 45
LweI GCATC 1 cut(s) 265
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 1 cut(s) 95
MalI GATC 6 cut(s) 122, 250, 267, 294, 314, 389
MboI GATC 6 cut(s) 120, 248, 265, 292, 312, 387
MboII GAAGA 4 cut(s) 64, 68, 280, 454
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 1 cut(s) 365
MluCI AATT 2 cut(s) 167, 395
MlyI GAGTC 2 cut(s) 96, 169
MnlI CCTC 4 cut(s) 52, 217, 328, 350
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 2 cut(s) 423, 460
MspA1I CMGCKG 1 cut(s) 36
MspR9I CCNGG 1 cut(s) 221
MvaI CCWGG 1 cut(s) 221
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 2 cut(s) 36, 417
NdeII GATC 6 cut(s) 120, 248, 265, 292, 312, 387
NlaIII CATG 3 cut(s) 244, 335, 388
NlaIV GGNNCC 2 cut(s) 48, 159
NmeAIII GCCGAG 1 cut(s) 296
NmuCI GTSAC 1 cut(s) 95
NruI TCGCGA 1 cut(s) 297
PagI TCATGA 2 cut(s) 240, 331
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 288
PdmI GAANNNNTTC 1 cut(s) 71
PkrI GCNGC 3 cut(s) 32, 35, 38
Ple19I CGATCG 1 cut(s) 295
PleI GAGTC 2 cut(s) 96, 169
PpsI GAGTC 2 cut(s) 96, 169
Psp1406I AACGTT 1 cut(s) 129
Psp6I CCWGG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 2 cut(s) 48, 159
PspPI GGNCC 1 cut(s) 337
PstNI CAGNNNCTG 1 cut(s) 178
PsuI RGATCY 1 cut(s) 265
PvuI CGATCG 1 cut(s) 295
RruI TCGCGA 1 cut(s) 297
RsaI GTAC 2 cut(s) 48, 63
RsaNI GTAC 2 cut(s) 47, 62
SaqAI TTAA 2 cut(s) 423, 460
SatI GCNGC 3 cut(s) 31, 34, 37
Sau3AI GATC 6 cut(s) 120, 248, 265, 292, 312, 387
Sau96I GGNCC 1 cut(s) 337
SchI GAGTC 2 cut(s) 96, 169
ScrFI CCNGG 1 cut(s) 221
SduI GDGCHC 1 cut(s) 365
SetI ASST 5 cut(s) 52, 63, 72, 132, 222
SfaNI GCATC 1 cut(s) 265
SfcI CTRYAG 1 cut(s) 366
Sse9I AATT 2 cut(s) 167, 395
SsiI CCGC 3 cut(s) 30, 36, 346
StyD4I CCNGG 1 cut(s) 219
TaaI ACNGT 2 cut(s) 20, 95
TaiI ACGT 1 cut(s) 132
TaqI TCGA 7 cut(s) 8, 75, 84, 192, 257, 282, 291
TasI AATT 2 cut(s) 167, 395
TauI GCSGC 2 cut(s) 33, 39
Tru1I TTAA 2 cut(s) 423, 460
Tru9I TTAA 2 cut(s) 423, 460
TscAI CASTG 2 cut(s) 100, 118
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 1 cut(s) 33
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 3 cut(s) 257, 320, 371
TspGWI ACGGA 3 cut(s) 114, 125, 260
TspRI CASTG 2 cut(s) 100, 118
XapI RAATTY 1 cut(s) 395
XmnI GAANNNNTTC 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.