Rroxscaffold_4G00317730

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002561
Physical Location & Seq
Reverse (-)
43200145 .. 43200606
462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00317730.1

Sequence Viewer

Length: 462 bp
ATGTCATCGAGTTTCAGCAGTTCAAGAAGTGGCAGCGGCATGGTGGTACCCATGGAAGAGGTACAGAAGGTCTTCAACAAGTTCGACGAGAATGGTGACGGACTCATCTCTTGCGATGAGCTCAAGAACGTTTTGTTTGAGCTGGGGTGCGAGATGAAGTCGGAGCAAGTGCAGACTGTGATGTCCGAGTTCGACAAGGACGGCGACGGCCACATTGACCTGGAGGAGTTCACAATACTCATGAATGGATCTGAGTCAAGCGAGGATATCCGTAACGCCTTCGATGTCTACGATCTCGACAAGAACGGCCTGATCTCGGCTAGCGAACTTCATGTGGTGCTCAACCGCTTAGGGAAGAAGTGCTCCGTTGGGGAATGTGCCACCATGATCACCAAATTTGACGCCAATGGAGATGGCTTCATTAACTTTCAAGAGTTCAAGAATATGTTGAACCGCCCCTAA

Protein Analysis

153

Amino Acids

16.99

Weight (kDa)

4.4

Isoelectric Point (pI)

50.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 19 - 81 1.2e-16 EF-hand domain pair
EF-hand_1 PF00036 20 - 45 8.7e-07 EF hand domain
EF-hand_6 PF13405 21 - 49 6.4e-07 EF-hand domain
EF-hand_8 PF13833 51 - 82 1.1e-06 EF-hand domain pair
EF-hand_1 PF00036 57 - 82 2.2e-07 EF hand domain
EF-hand_7 PF13499 88 - 149 5.9e-14 EF-hand domain pair
EF-hand_6 PF13405 89 - 118 4.7e-06 EF-hand domain
EF-hand_8 PF13833 117 - 152 4.9e-06 EF-hand domain pair
EF-hand_1 PF00036 126 - 151 4.5e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 181
Acc65I GGTACC 1 cut(s) 46
AccB1I GGYRCC 1 cut(s) 46
AccI GTMKAC 1 cut(s) 288
AciI CCGC 3 cut(s) 36, 346, 454
AclI AACGTT 1 cut(s) 129
AclWI GGATC 1 cut(s) 256
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 1 cut(s) 395
AcyI GRCGYC 1 cut(s) 402
AfaI GTAC 2 cut(s) 48, 63
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 5 cut(s) 24, 76, 431, 439, 451
AjnI CCWGG 1 cut(s) 219
AluBI AGCT 2 cut(s) 121, 142
AluI AGCT 2 cut(s) 121, 142
Alw21I GWGCWC 3 cut(s) 123, 342, 365
AlwI GGATC 1 cut(s) 256
AoxI GGCC 2 cut(s) 208, 307
ApeKI GCWGC 1 cut(s) 33
ApoI RAATTY 1 cut(s) 395
Asp700I GAANNNNTTC 1 cut(s) 71
Asp718I GGTACC 1 cut(s) 46
AsuHPI GGTGA 2 cut(s) 107, 382
AsuNHI GCTAGC 1 cut(s) 320
BanI GGYRCC 1 cut(s) 46
BanII GRGCYC 1 cut(s) 123
BbsI GAAGAC 1 cut(s) 64
Bbv12I GWGCWC 3 cut(s) 123, 342, 365
BbvI GCAGC 1 cut(s) 45
BccI CCATC 1 cut(s) 407
BceAI ACGGC 3 cut(s) 217, 223, 322
BciT130I CCWGG 1 cut(s) 221
BclI TGATCA 1 cut(s) 387
BfaI CTAG 1 cut(s) 321
BisI GCNGC 2 cut(s) 34, 37
BlsI GCNGC 2 cut(s) 35, 38
Bme1390I CCNGG 1 cut(s) 221
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 1 cut(s) 221
BmtI GCTAGC 1 cut(s) 324
BpiI GAAGAC 1 cut(s) 64
BpmI CTGGAG 1 cut(s) 242
Bpu10I CCTNAGC 1 cut(s) 349
BpuEI CTTGAG 1 cut(s) 107
BsaHI GRCGYC 1 cut(s) 402
BsaJI CCNNGG 1 cut(s) 51
Bsc4I CCNNNNNNNGG 1 cut(s) 316
BseBI CCWGG 1 cut(s) 221
BseDI CCNNGG 1 cut(s) 51
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMII CTCAG 1 cut(s) 243
BseRI GAGGAG 1 cut(s) 239
BseXI GCAGC 1 cut(s) 45
BseYI CCCAGC 1 cut(s) 142
BsgI GTGCAG 1 cut(s) 191
BshFI GGCC 2 cut(s) 210, 309
BshNI GGYRCC 1 cut(s) 46
BsiHKAI GWGCWC 3 cut(s) 123, 342, 365
BslI CCNNNNNNNGG 1 cut(s) 316
BsnI GGCC 2 cut(s) 210, 309
Bsp1286I GDGCHC 3 cut(s) 123, 342, 365
Bsp143I GATC 4 cut(s) 248, 292, 312, 387
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 3 cut(s) 36, 346, 454
BspANI GGCC 2 cut(s) 210, 309
BspCNI CTCAG 1 cut(s) 244
BspHI TCATGA 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 48
BspOI GCTAGC 1 cut(s) 324
BspPI GGATC 1 cut(s) 256
BspT107I GGYRCC 1 cut(s) 46
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 4 cut(s) 248, 292, 312, 387
BssNI GRCGYC 1 cut(s) 402
BssT1I CCWWGG 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 1 cut(s) 178
Bst6I CTCTTC 1 cut(s) 51
BstACI GRCGYC 1 cut(s) 402
BstC8I GCNNGC 1 cut(s) 322
BstDEI CTNAG 2 cut(s) 252, 349
BstDSI CCRYGG 1 cut(s) 51
BstKTI GATC 4 cut(s) 251, 295, 315, 390
BstMBI GATC 4 cut(s) 248, 292, 312, 387
BstNI CCWGG 1 cut(s) 221
BstSCI CCNGG 1 cut(s) 219
BstV1I GCAGC 1 cut(s) 45
BstV2I GAAGAC 1 cut(s) 64
BstX2I RGATCY 1 cut(s) 248
BstYI RGATCY 1 cut(s) 248
BsuRI GGCC 2 cut(s) 210, 309
BtgI CCRYGG 1 cut(s) 51
BtgZI GCGATG 1 cut(s) 129
Cac8I GCNNGC 1 cut(s) 322
CciI TCATGA 1 cut(s) 240
CseI GACGC 1 cut(s) 410
Csp6I GTAC 2 cut(s) 47, 62
CviAII CATG 5 cut(s) 40, 52, 241, 332, 385
CviJI RGCY 6 cut(s) 121, 142, 210, 309, 320, 417
CviKI_1 RGCY 6 cut(s) 121, 142, 210, 309, 320, 417
CviQI GTAC 2 cut(s) 47, 62
DdeI CTNAG 2 cut(s) 252, 349
DpnI GATC 4 cut(s) 250, 294, 314, 389
DpnII GATC 4 cut(s) 248, 292, 312, 387
DrdI GACNNNNNNGTC 1 cut(s) 181
DseDI GACNNNNNNGTC 1 cut(s) 181
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Ecl136II GAGCTC 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 123
Eco32I GATATC 1 cut(s) 268
Eco53kI GAGCTC 1 cut(s) 121
EcoICRI GAGCTC 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 219
EcoRV GATATC 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 5 cut(s) 43, 55, 244, 335, 388
FaiI YATR 6 cut(s) 41, 53, 242, 333, 386, 446
FatI CATG 5 cut(s) 39, 51, 240, 331, 384
FbaI TGATCA 1 cut(s) 387
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 2 cut(s) 34, 37
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 2 cut(s) 34, 37
FspBI CTAG 1 cut(s) 321
GluI GCNGC 2 cut(s) 34, 37
GsaI CCCAGC 1 cut(s) 146
GsuI CTGGAG 1 cut(s) 242
HaeIII GGCC 2 cut(s) 210, 309
HgaI GACGC 1 cut(s) 410
Hin1I GRCGYC 1 cut(s) 402
Hin1II CATG 5 cut(s) 43, 55, 244, 335, 388
HinfI GANTC 2 cut(s) 102, 254
HphI GGTGA 2 cut(s) 107, 382
Hpy166II GTNNAC 2 cut(s) 231, 289
Hpy188I TCNGA 3 cut(s) 163, 187, 253
Hpy188III TCNNGA 6 cut(s) 24, 124, 241, 296, 431, 439
Hpy8I GTNNAC 2 cut(s) 231, 289
Hpy99I CGWCG 2 cut(s) 89, 209
HpyAV CCTTC 2 cut(s) 61, 289
HpyCH4III ACNGT 1 cut(s) 178
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 1 cut(s) 172
HpyF3I CTNAG 2 cut(s) 252, 349
HpySE526I ACGT 1 cut(s) 129
Hsp92I GRCGYC 1 cut(s) 402
Hsp92II CATG 5 cut(s) 43, 55, 244, 335, 388
KpnI GGTACC 1 cut(s) 50
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 4 cut(s) 248, 292, 312, 387
LmnI GCTCC 2 cut(s) 163, 368
LpnPI CCDG 4 cut(s) 128, 206, 233, 323
Lsp1109I GCAGC 1 cut(s) 45
MaeI CTAG 1 cut(s) 321
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 2 cut(s) 95, 272
MalI GATC 4 cut(s) 250, 294, 314, 389
MboI GATC 4 cut(s) 248, 292, 312, 387
MboII GAAGA 3 cut(s) 64, 68, 367
MflI RGATCY 1 cut(s) 248
MhlI GDGCHC 3 cut(s) 123, 342, 365
MluCI AATT 1 cut(s) 395
MlyI GAGTC 2 cut(s) 96, 263
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 3 cut(s) 52, 217, 256
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 1 cut(s) 423
MspA1I CMGCKG 1 cut(s) 36
MspR9I CCNGG 1 cut(s) 221
MvaI CCWGG 1 cut(s) 221
NcoI CCATGG 1 cut(s) 51
NdeII GATC 4 cut(s) 248, 292, 312, 387
NheI GCTAGC 1 cut(s) 320
NlaIII CATG 5 cut(s) 43, 55, 244, 335, 388
NlaIV GGNNCC 1 cut(s) 48
NmeAIII GCCGAG 1 cut(s) 296
NmuCI GTSAC 1 cut(s) 95
PagI TCATGA 1 cut(s) 240
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PdmI GAANNNNTTC 1 cut(s) 71
PkrI GCNGC 2 cut(s) 35, 38
PleI GAGTC 2 cut(s) 96, 262
PpsI GAGTC 2 cut(s) 96, 262
Psp124BI GAGCTC 1 cut(s) 123
Psp1406I AACGTT 1 cut(s) 129
Psp6I CCWGG 1 cut(s) 219
PspFI CCCAGC 1 cut(s) 142
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 1 cut(s) 48
PsuI RGATCY 1 cut(s) 248
RsaI GTAC 2 cut(s) 48, 63
RsaNI GTAC 2 cut(s) 47, 62
SacI GAGCTC 1 cut(s) 123
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 2 cut(s) 34, 37
Sau3AI GATC 4 cut(s) 248, 292, 312, 387
SchI GAGTC 2 cut(s) 96, 263
ScrFI CCNGG 1 cut(s) 221
SduI GDGCHC 3 cut(s) 123, 342, 365
SetI ASST 6 cut(s) 63, 72, 123, 132, 144, 222
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 1 cut(s) 395
SsiI CCGC 3 cut(s) 36, 346, 454
SspMI CTAG 1 cut(s) 321
SstI GAGCTC 1 cut(s) 123
StyD4I CCNGG 1 cut(s) 219
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 178
TaiI ACGT 1 cut(s) 132
TaqI TCGA 5 cut(s) 8, 84, 192, 282, 297
TasI AATT 1 cut(s) 395
TauI GCSGC 1 cut(s) 39
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 1 cut(s) 33
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 4 cut(s) 170, 257, 320, 409
TspGWI ACGGA 3 cut(s) 114, 260, 355
XapI RAATTY 1 cut(s) 395
XmiI GTMKAC 1 cut(s) 288
XmnI GAANNNNTTC 1 cut(s) 71
XspI CTAG 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.