Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0002561

Physical Location & Seq

Genomic Coordinates Reverse (-)

27780387 .. 27780845

Gene Structure 459 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G012520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 459 bp

ATGTCATCGAGTTTCAACAGTTCAAAAAGCGGCAGCAGCGTAGTGGTATCTATGGAAGAGGTCTTCAACAAGTTCGATGAGAACAGTGACGGACTCATCTCCAGTGATGAGCTCAAGAACGTTTTCCGTAAGATGGGGCCGGAGATGAAGCCAGAGGAAGTGCAGCCTGTGATTTCTGAGTTCGACAAGGACGGGGATGGCCACATCGACCTGGAGGAGTTCACAAAAATCATGAATGGATCGAGCAAGGATCTCCTTGACGCCTTGCGTGACGCCTTCAATATGTACGATCTCGACAAGAATGGCCTGATCTCGGCGAGCGAACTTCATGAGGTGCTCAACCGCTTAGGGGAGAAGTGCTCCGTTGGGGAATGTGCCACCATGATCACCAAATTTGACGTCAATGGTGATGGCTTCATCAACTTTGAAGAGTTCAAGCAGATGATGAACCGCTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000302 GO:0001101 GO:0001505 GO:0002376 GO:0003006 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009409 GO:0009416 GO:0009605 GO:0009612 GO:0009628 GO:0009636 GO:0009642 GO:0009646 GO:0009648 GO:0009719 GO:0009725 GO:0009733 GO:0009737 GO:0009791 GO:0009909 GO:0010033 GO:0010035 GO:0010038 GO:0010228 GO:0016020 GO:0019222 GO:0022414 GO:0031323 GO:0032501 GO:0032502 GO:0033993 GO:0042221 GO:0042493 GO:0042542 GO:0043167 GO:0043169 GO:0044464 GO:0045087 GO:0046677 GO:0046872 GO:0048571 GO:0048573 GO:0048574 GO:0048580 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051239 GO:0051592 GO:0061458 GO:0065007 GO:0065008 GO:0071944 GO:0080164 GO:0097305 GO:1901700 GO:2000026 GO:2000241 GO:2000377

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1

Protein Analysis

152

Amino Acids

17.0

Weight (kDa)

4.45

Isoelectric Point (pI)

47.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	18 - 78	2e-16	EF-hand domain pair
EF-hand_6	PF13405	18 - 46	3.2e-07	EF-hand domain
EF-hand_1	PF00036	19 - 45	2.1e-06	EF hand domain
EF-hand_8	PF13833	29 - 75	5.1e-08	EF-hand domain pair
EF-hand_1	PF00036	57 - 79	1.5e-07	EF hand domain
EF-hand_5	PF13202	58 - 78	1e-05	EF hand
EF-hand_7	PF13499	87 - 149	1.1e-15	EF-hand domain pair
EF-hand_1	PF00036	89 - 115	2.5e-06	EF hand domain
EF-hand_6	PF13405	89 - 117	3.1e-07	EF-hand domain
EF-hand_5	PF13202	89 - 112	1e-05	EF hand
EF-hand_8	PF13833	115 - 151	5.6e-07	EF-hand domain pair
EF-hand_1	PF00036	125 - 151	4.1e-09	EF hand domain
EF-hand_5	PF13202	131 - 149	1.2e-06	EF hand

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002561)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g28870 FvH4_7g07200
rosa_chinensis	RchiOBHm_Chr1g0337231 RchiOBHm_Chr3g0493331 RchiOBHm_Chr6g0305841
rosa_laevigata	RLG00000022718 RLG00000029296 RLG00000029299 RLG00000029490 RLG00000033995
rosa_multiflora	Rmu_co8149994.1_g000001 Rmu_sc0000828.1_g000005 Rmu_sc0004469.1_g000017 Rmu_sc0006010.1_g000003 Rmu_sc0007874.1_g000001 Rmu_sc0017606.1_g000006
rosa_roxburghii	Rroxscaffold_4G00314650 Rroxscaffold_4G00314700 Rroxscaffold_4G00317730 Rroxscaffold_6G00391630
rosa_rugosa	Rorug01G0128400 Rorug01G0132200.1 Rorug03G0260500 Rorug06G0344300.1
rosa_samantha	Rh1BG117500 Rh1BG121800 Rh1CG099300 Rh1CG107500 Rh1CG143900 Rh1DG106500 Rh1DG116300 Rh1DG160500 Rh3AG309600 Rh3CG342900 Rh3DG344700 Rh6AG456100 Rh6CG470100 Rh6DG456800
rosa_wichuraiana	Rw1G012520 Rw3G027190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	402
AccBSI	CCGCTC	1 cut(s)	453
AciI	CCGC	3 cut(s)	30, 343, 451
AclI	AACGTT	1 cut(s)	120
AclWI	GGATC	2 cut(s)	247, 258
AcoI	YGGCCR	1 cut(s)	199
AcsI	RAATTY	1 cut(s)	392
AcyI	GRCGYC	3 cut(s)	261, 273, 399
AfaI	GTAC	1 cut(s)	287
AfiI	CCNNNNNNNGG	3 cut(s)	133, 313, 349
AgsI	TTSAA	6 cut(s)	16, 24, 67, 280, 428, 436
AjnI	CCWGG	1 cut(s)	210
AluBI	AGCT	1 cut(s)	112
AluI	AGCT	1 cut(s)	112
Alw21I	GWGCWC	3 cut(s)	114, 339, 362
AlwI	GGATC	2 cut(s)	247, 258
AoxI	GGCC	3 cut(s)	137, 199, 304
ApeKI	GCWGC	3 cut(s)	33, 36, 163
ApoI	RAATTY	1 cut(s)	392
ArsI	GACNNNNNNTTYG	2 cut(s)	384, 416
Asp700I	GAANNNNTTC	1 cut(s)	122
AspS9I	GGNCC	1 cut(s)	137
AsuHPI	GGTGA	2 cut(s)	379, 419
BalI	TGGCCA	1 cut(s)	201
BanII	GRGCYC	1 cut(s)	114
BbsI	GAAGAC	1 cut(s)	55
Bbv12I	GWGCWC	3 cut(s)	114, 339, 362
BbvI	GCAGC	3 cut(s)	45, 48, 175
BccI	CCATC	3 cut(s)	127, 191, 404
BciT130I	CCWGG	1 cut(s)	212
BclI	TGATCA	1 cut(s)	384
BisI	GCNGC	4 cut(s)	31, 34, 37, 164
BlsI	GCNGC	4 cut(s)	32, 35, 38, 165
Bme1390I	CCNGG	1 cut(s)	212
BmgT120I	GGNCC	1 cut(s)	137
BmiI	GGNNCC	1 cut(s)	138
BmrFI	CCNGG	1 cut(s)	212
BpiI	GAAGAC	1 cut(s)	55
BplI	GAGNNNNNCTC	2 cut(s)	344, 376
BpmI	CTGGAG	2 cut(s)	85, 233
Bpu10I	CCTNAGC	1 cut(s)	346
BpuEI	CTTGAG	1 cut(s)	98
BsaHI	GRCGYC	3 cut(s)	261, 273, 399
Bsc4I	CCNNNNNNNGG	3 cut(s)	133, 313, 349
Bse1I	ACTGG	1 cut(s)	102
BseBI	CCWGG	1 cut(s)	212
BseGI	GGATG	1 cut(s)	202
BseLI	CCNNNNNNNGG	3 cut(s)	133, 313, 349
BseMII	CTCAG	1 cut(s)	168
BseNI	ACTGG	1 cut(s)	102
BseRI	GAGGAG	1 cut(s)	230
BseXI	GCAGC	3 cut(s)	45, 48, 175
BsgI	GTGCAG	1 cut(s)	182
BshFI	GGCC	3 cut(s)	139, 201, 306
BsiHKAI	GWGCWC	3 cut(s)	114, 339, 362
BsiSI	CCGG	1 cut(s)	140
BslI	CCNNNNNNNGG	3 cut(s)	133, 313, 349
BsnI	GGCC	3 cut(s)	139, 201, 306
Bsp1286I	GDGCHC	3 cut(s)	114, 339, 362
Bsp143I	GATC	5 cut(s)	239, 250, 289, 309, 384
BspACI	CCGC	3 cut(s)	30, 343, 451
BspANI	GGCC	3 cut(s)	139, 201, 306
BspCNI	CTCAG	1 cut(s)	169
BspHI	TCATGA	2 cut(s)	231, 328
BspLI	GGNNCC	1 cut(s)	138
BspPI	GGATC	2 cut(s)	247, 258
BsrBI	CCGCTC	1 cut(s)	453
BsrI	ACTGG	1 cut(s)	102
BssMI	GATC	5 cut(s)	239, 250, 289, 309, 384
BssNI	GRCGYC	3 cut(s)	261, 273, 399
Bst2UI	CCWGG	1 cut(s)	212
Bst4CI	ACNGT	2 cut(s)	20, 86
Bst6I	CTCTTC	2 cut(s)	51, 423
BstACI	GRCGYC	3 cut(s)	261, 273, 399
BstC8I	GCNNGC	1 cut(s)	319
BstDEI	CTNAG	2 cut(s)	177, 346
BstF5I	GGATG	1 cut(s)	202
BstKTI	GATC	5 cut(s)	242, 253, 292, 312, 387
BstMBI	GATC	5 cut(s)	239, 250, 289, 309, 384
BstMWI	GCNNNNNNNGC	1 cut(s)	36
BstNI	CCWGG	1 cut(s)	212
BstSCI	CCNGG	1 cut(s)	210
BstV1I	GCAGC	3 cut(s)	45, 48, 175
BstV2I	GAAGAC	1 cut(s)	55
BstX2I	RGATCY	1 cut(s)	250
BstYI	RGATCY	1 cut(s)	250
BsuRI	GGCC	3 cut(s)	139, 201, 306
BtsCI	GGATG	1 cut(s)	202
BtsIMutI	CAGTG	2 cut(s)	91, 109
Cac8I	GCNNGC	1 cut(s)	319
CciI	TCATGA	2 cut(s)	231, 328
Cfr13I	GGNCC	1 cut(s)	137
CseI	GACGC	2 cut(s)	269, 281
Csp6I	GTAC	1 cut(s)	286
CviAII	CATG	3 cut(s)	232, 329, 382
CviJI	RGCY	7 cut(s)	112, 139, 151, 166, 201, 306, 414
CviKI_1	RGCY	7 cut(s)	112, 139, 151, 166, 201, 306, 414
CviQI	GTAC	1 cut(s)	286
DdeI	CTNAG	2 cut(s)	177, 346
DpnI	GATC	5 cut(s)	241, 252, 291, 311, 386
DpnII	GATC	5 cut(s)	239, 250, 289, 309, 384
EaeI	YGGCCR	1 cut(s)	199
Eam1104I	CTCTTC	2 cut(s)	51, 423
EarI	CTCTTC	2 cut(s)	51, 423
Ecl136II	GAGCTC	1 cut(s)	112
Eco24I	GRGCYC	1 cut(s)	114
Eco53kI	GAGCTC	1 cut(s)	112
EcoICRI	GAGCTC	1 cut(s)	112
EcoRII	CCWGG	1 cut(s)	210
EcoT38I	GRGCYC	1 cut(s)	114
FaeI	CATG	3 cut(s)	235, 332, 385
FaiI	YATR	5 cut(s)	53, 233, 284, 330, 383
FatI	CATG	3 cut(s)	231, 328, 381
FbaI	TGATCA	1 cut(s)	384
Fnu4HI	GCNGC	4 cut(s)	31, 34, 37, 164
FokI	GGATG	1 cut(s)	209
FriOI	GRGCYC	1 cut(s)	114
Fsp4HI	GCNGC	4 cut(s)	31, 34, 37, 164
GluI	GCNGC	4 cut(s)	31, 34, 37, 164
GsuI	CTGGAG	2 cut(s)	85, 233
HaeIII	GGCC	3 cut(s)	139, 201, 306
HapII	CCGG	1 cut(s)	140
HgaI	GACGC	2 cut(s)	269, 281
Hin1I	GRCGYC	3 cut(s)	261, 273, 399
Hin1II	CATG	3 cut(s)	235, 332, 385
HinfI	GANTC	1 cut(s)	93
HpaII	CCGG	1 cut(s)	140
HphI	GGTGA	2 cut(s)	379, 419
Hpy166II	GTNNAC	1 cut(s)	222
Hpy188I	TCNGA	1 cut(s)	178
Hpy188III	TCNNGA	5 cut(s)	115, 232, 293, 329, 456
Hpy8I	GTNNAC	1 cut(s)	222
HpyAV	CCTTC	1 cut(s)	286
HpyCH4III	ACNGT	2 cut(s)	20, 86
HpyCH4IV	ACGT	2 cut(s)	120, 399
HpyCH4V	TGCA	1 cut(s)	163
HpyF10VI	GCNNNNNNNGC	1 cut(s)	36
HpyF3I	CTNAG	2 cut(s)	177, 346
HpySE526I	ACGT	2 cut(s)	120, 399
Hsp92I	GRCGYC	3 cut(s)	261, 273, 399
Hsp92II	CATG	3 cut(s)	235, 332, 385
Ksp22I	TGATCA	1 cut(s)	384
Kzo9I	GATC	5 cut(s)	239, 250, 289, 309, 384
LmnI	GCTCC	1 cut(s)	365
LpnPI	CCDG	7 cut(s)	115, 153, 165, 180, 197, 224, 320
Lsp1109I	GCAGC	3 cut(s)	45, 48, 175
MaeII	ACGT	2 cut(s)	120, 399
MaeIII	GTNAC	2 cut(s)	86, 269
MalI	GATC	5 cut(s)	241, 252, 291, 311, 386
MbiI	CCGCTC	1 cut(s)	453
MboI	GATC	5 cut(s)	239, 250, 289, 309, 384
MboII	GAAGA	3 cut(s)	55, 68, 440
MflI	RGATCY	1 cut(s)	250
MhlI	GDGCHC	3 cut(s)	114, 339, 362
MlsI	TGGCCA	1 cut(s)	201
MluCI	AATT	1 cut(s)	392
MluNI	TGGCCA	1 cut(s)	201
MlyI	GAGTC	1 cut(s)	87
MnlI	CCTC	4 cut(s)	52, 148, 208, 325
Mox20I	TGGCCA	1 cut(s)	201
MroXI	GAANNNNTTC	1 cut(s)	122
MscI	TGGCCA	1 cut(s)	201
Msp20I	TGGCCA	1 cut(s)	201
MspI	CCGG	1 cut(s)	140
MspR9I	CCNGG	1 cut(s)	212
MvaI	CCWGG	1 cut(s)	212
MwoI	GCNNNNNNNGC	1 cut(s)	36
NdeII	GATC	5 cut(s)	239, 250, 289, 309, 384
NlaIII	CATG	3 cut(s)	235, 332, 385
NlaIV	GGNNCC	1 cut(s)	138
NmeAIII	GCCGAG	1 cut(s)	293
NmuCI	GTSAC	2 cut(s)	86, 269
PagI	TCATGA	2 cut(s)	231, 328
PdmI	GAANNNNTTC	1 cut(s)	122
PkrI	GCNGC	4 cut(s)	32, 35, 38, 165
PleI	GAGTC	1 cut(s)	87
PpsI	GAGTC	1 cut(s)	87
Psp124BI	GAGCTC	1 cut(s)	114
Psp1406I	AACGTT	1 cut(s)	120
Psp6I	CCWGG	1 cut(s)	210
PspGI	CCWGG	1 cut(s)	210
PspN4I	GGNNCC	1 cut(s)	138
PspPI	GGNCC	1 cut(s)	137
PsuI	RGATCY	1 cut(s)	250
RsaI	GTAC	1 cut(s)	287
RsaNI	GTAC	1 cut(s)	286
SacI	GAGCTC	1 cut(s)	114
SatI	GCNGC	4 cut(s)	31, 34, 37, 164
Sau3AI	GATC	5 cut(s)	239, 250, 289, 309, 384
Sau96I	GGNCC	1 cut(s)	137
SchI	GAGTC	1 cut(s)	87
ScrFI	CCNGG	1 cut(s)	212
SduI	GDGCHC	3 cut(s)	114, 339, 362
SetI	ASST	6 cut(s)	63, 114, 123, 213, 336, 402
SmlI	CTYRAG	1 cut(s)	113
SmoI	CTYRAG	1 cut(s)	113
Sse9I	AATT	1 cut(s)	392
SsiI	CCGC	3 cut(s)	30, 343, 451
SstI	GAGCTC	1 cut(s)	114
StyD4I	CCNGG	1 cut(s)	210
TaaI	ACNGT	2 cut(s)	20, 86
TaiI	ACGT	2 cut(s)	123, 402
TaqI	TCGA	6 cut(s)	8, 75, 183, 207, 242, 294
TasI	AATT	1 cut(s)	392
TauI	GCSGC	1 cut(s)	33
TscAI	CASTG	2 cut(s)	91, 109
TseFI	GTSAC	2 cut(s)	86, 269
TseI	GCWGC	3 cut(s)	33, 36, 163
Tsp45I	GTSAC	2 cut(s)	86, 269
TspDTI	ATGAA	4 cut(s)	161, 248, 317, 406
TspGWI	ACGGA	3 cut(s)	105, 116, 352
TspRI	CASTG	2 cut(s)	91, 109
XapI	RAATTY	1 cut(s)	392
XmnI	GAANNNNTTC	1 cut(s)	122
ZraI	GACGTC	1 cut(s)	400

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.