Rorug03G0260500

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002561
Physical Location & Seq
Forward (+)
25044666 .. 25045097
432 bp
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UTR
Exon/CDS
Intron
Rorug03G0260500.1

Sequence Viewer

Length: 432 bp
ATGGACGCGAAACTCATCGACCAAACGTGCAGAAAAACACCGGATTACAACCTTTGTACCTCAGTTCTCAAATCTAGCCCCGATAAAGATCTTAGAGGTTTAGCTATCGTAGCTATTGATGCAGTTGGGTCTAAAGCAACTACTACAATGAACAGAGTTAATGAGTTGCTTAAACAAAGCCCAAAAGATACTGCCTTAAATCATTGTCATGATATGTACAATGTGATTTTAAATTCATATGTTCAAGGAGCCAGGGATACGCTTAAGCGGGGTGCCTCTTCTCATATTGCAACCTCATGCATGAGTCTCACTCCCGATACTGTAAGGTCATGCGAGGAAGCTTTTAAAGGCAGGTCTCCTCTTACTAATTTGAACAAAGATGTCGAGAATATTGCGGCAGTGGCTGGAGCAGTGGCGGGGCAATTGCCTTGA

Protein Analysis

143

Amino Acids

15.36

Weight (kDa)

8.29

Isoelectric Point (pI)

50.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 4 - 133 1.9e-18 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 342
AccB1I GGYRCC 1 cut(s) 272
AccII CGCG 1 cut(s) 8
AciI CCGC 3 cut(s) 268, 395, 416
AcsI RAATTY 1 cut(s) 232
AfaI GTAC 2 cut(s) 58, 218
AflII CTTAAG 1 cut(s) 263
AgsI TTSAA 2 cut(s) 245, 373
AjnI CCWGG 1 cut(s) 251
AluBI AGCT 3 cut(s) 104, 113, 341
AluI AGCT 3 cut(s) 104, 113, 341
Alw26I GTCTC 2 cut(s) 311, 360
AlwNI CAGNNNCTG 1 cut(s) 404
ApoI RAATTY 1 cut(s) 232
BaeI ACNNNNGTAYC 2 cut(s) 40, 73
BanI GGYRCC 1 cut(s) 272
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciT130I CCWGG 1 cut(s) 253
BciVI GTATCC 1 cut(s) 250
BcoDI GTCTC 2 cut(s) 311, 360
BfaI CTAG 1 cut(s) 75
BfrI CTTAAG 1 cut(s) 263
BfuAI ACCTGC 1 cut(s) 342
BfuI GTATCC 1 cut(s) 250
BglII AGATCT 1 cut(s) 88
BisI GCNGC 1 cut(s) 396
BlsI GCNGC 1 cut(s) 397
Bme1390I CCNGG 1 cut(s) 253
BmiI GGNNCC 2 cut(s) 250, 274
BmrFI CCNGG 1 cut(s) 253
BmsI GCATC 1 cut(s) 109
BplI GAGNNNNNCTC 2 cut(s) 295, 327
BpmI CTGGAG 1 cut(s) 426
BsaBI GATNNNNATC 1 cut(s) 87
BsaI GGTCTC 1 cut(s) 360
BsaJI CCNNGG 1 cut(s) 252
BsaWI WCCGGW 1 cut(s) 40
Bse8I GATNNNNATC 1 cut(s) 87
BseBI CCWGG 1 cut(s) 253
BseDI CCNNGG 1 cut(s) 252
BseJI GATNNNNATC 1 cut(s) 87
BseMII CTCAG 1 cut(s) 75
BseRI GAGGAG 1 cut(s) 348
BsgI GTGCAG 1 cut(s) 49
Bsh1236I CGCG 1 cut(s) 8
BshNI GGYRCC 1 cut(s) 272
BsiSI CCGG 1 cut(s) 41
BsmAI GTCTC 2 cut(s) 311, 360
Bso31I GGTCTC 1 cut(s) 360
Bsp1407I TGTACA 1 cut(s) 216
Bsp143I GATC 1 cut(s) 88
BspACI CCGC 3 cut(s) 268, 395, 416
BspCNI CTCAG 1 cut(s) 74
BspFNI CGCG 1 cut(s) 8
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 2 cut(s) 250, 274
BspMI ACCTGC 1 cut(s) 342
BspT107I GGYRCC 1 cut(s) 272
BspTI CTTAAG 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 360
BsrGI TGTACA 1 cut(s) 216
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 253
Bst4CI ACNGT 1 cut(s) 322
Bst6I CTCTTC 1 cut(s) 283
BstAFI CTTAAG 1 cut(s) 263
BstAUI TGTACA 1 cut(s) 216
BstDEI CTNAG 2 cut(s) 61, 92
BstFNI CGCG 1 cut(s) 8
BstKTI GATC 1 cut(s) 91
BstMAI GTCTC 2 cut(s) 311, 360
BstMBI GATC 1 cut(s) 88
BstMWI GCNNNNNNNGC 3 cut(s) 110, 119, 401
BstNI CCWGG 1 cut(s) 253
BstSCI CCNGG 1 cut(s) 251
BstUI CGCG 1 cut(s) 8
BstX2I RGATCY 1 cut(s) 88
BstYI RGATCY 1 cut(s) 88
BsuI GTATCC 1 cut(s) 250
BtsI GCAGTG 2 cut(s) 405, 417
BtsIMutI CAGTG 2 cut(s) 405, 417
BveI ACCTGC 1 cut(s) 342
CaiI CAGNNNCTG 1 cut(s) 404
CciI TCATGA 1 cut(s) 208
CseI GACGC 1 cut(s) 14
Csp6I GTAC 2 cut(s) 57, 217
CviAII CATG 4 cut(s) 209, 297, 301, 330
CviJI RGCY 7 cut(s) 78, 104, 113, 180, 251, 341, 404
CviKI_1 RGCY 7 cut(s) 78, 104, 113, 180, 251, 341, 404
CviQI GTAC 2 cut(s) 57, 217
DdeI CTNAG 2 cut(s) 61, 92
DpnI GATC 1 cut(s) 90
DpnII GATC 1 cut(s) 88
DraI TTTAAA 2 cut(s) 231, 346
Eam1104I CTCTTC 1 cut(s) 283
EarI CTCTTC 1 cut(s) 283
Eco31I GGTCTC 1 cut(s) 360
EcoRII CCWGG 1 cut(s) 251
EcoT22I ATGCAT 1 cut(s) 302
FaeI CATG 4 cut(s) 212, 300, 304, 333
FaiI YATR 8 cut(s) 210, 215, 238, 240, 285, 298, 302, 331
FatI CATG 4 cut(s) 208, 296, 300, 329
FauI CCCGC 2 cut(s) 261, 409
FauNDI CATATG 1 cut(s) 238
Fnu4HI GCNGC 1 cut(s) 396
Fsp4HI GCNGC 1 cut(s) 396
FspBI CTAG 1 cut(s) 75
GluI GCNGC 1 cut(s) 396
GsuI CTGGAG 1 cut(s) 426
HapII CCGG 1 cut(s) 41
HgaI GACGC 1 cut(s) 14
Hin1II CATG 4 cut(s) 212, 300, 304, 333
HindIII AAGCTT 1 cut(s) 339
HinfI GANTC 1 cut(s) 304
HpaII CCGG 1 cut(s) 41
Hpy188III TCNNGA 3 cut(s) 209, 314, 385
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4IV ACGT 1 cut(s) 26
HpyCH4V TGCA 4 cut(s) 30, 122, 290, 300
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 119, 401
HpyF3I CTNAG 2 cut(s) 61, 92
HpySE526I ACGT 1 cut(s) 26
Hsp92II CATG 4 cut(s) 212, 300, 304, 333
Kzo9I GATC 1 cut(s) 88
LmnI GCTCC 2 cut(s) 248, 407
LpnPI CCDG 5 cut(s) 54, 238, 265, 337, 390
LweI GCATC 1 cut(s) 109
MaeI CTAG 1 cut(s) 75
MaeII ACGT 1 cut(s) 26
MalI GATC 1 cut(s) 90
MboI GATC 1 cut(s) 88
MboII GAAGA 1 cut(s) 270
MfeI CAATTG 1 cut(s) 422
MflI RGATCY 1 cut(s) 88
MluCI AATT 3 cut(s) 232, 367, 422
MlyI GAGTC 1 cut(s) 313
MnlI CCTC 6 cut(s) 70, 89, 286, 304, 328, 369
Mph1103I ATGCAT 1 cut(s) 302
MseI TTAA 6 cut(s) 159, 171, 197, 230, 264, 345
MslI CAYNNNNRTG 1 cut(s) 207
MspCI CTTAAG 1 cut(s) 263
MspI CCGG 1 cut(s) 41
MspR9I CCNGG 1 cut(s) 253
MunI CAATTG 1 cut(s) 422
MvaI CCWGG 1 cut(s) 253
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 3 cut(s) 110, 119, 401
NdeI CATATG 1 cut(s) 238
NdeII GATC 1 cut(s) 88
NlaIII CATG 4 cut(s) 212, 300, 304, 333
NlaIV GGNNCC 2 cut(s) 250, 274
NsiI ATGCAT 1 cut(s) 302
PagI TCATGA 1 cut(s) 208
PkrI GCNGC 1 cut(s) 397
PleI GAGTC 1 cut(s) 312
PpsI GAGTC 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 251
PspGI CCWGG 1 cut(s) 251
PspN4I GGNNCC 2 cut(s) 250, 274
PstNI CAGNNNCTG 1 cut(s) 404
PsuI RGATCY 1 cut(s) 88
RsaI GTAC 2 cut(s) 58, 218
RsaNI GTAC 2 cut(s) 57, 217
RseI CAYNNNNRTG 1 cut(s) 207
SaqAI TTAA 6 cut(s) 159, 171, 197, 230, 264, 345
SatI GCNGC 1 cut(s) 396
Sau3AI GATC 1 cut(s) 88
SchI GAGTC 1 cut(s) 313
ScrFI CCNGG 1 cut(s) 253
SfaNI GCATC 1 cut(s) 109
SmiMI CAYNNNNRTG 1 cut(s) 207
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 3 cut(s) 232, 367, 422
SsiI CCGC 3 cut(s) 268, 395, 416
SspI AATATT 1 cut(s) 391
SspMI CTAG 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 251
TaaI ACNGT 1 cut(s) 322
TaiI ACGT 1 cut(s) 29
TaqI TCGA 2 cut(s) 18, 384
TasI AATT 3 cut(s) 232, 367, 422
TatI WGTACW 1 cut(s) 216
TauI GCSGC 1 cut(s) 398
Tru1I TTAA 6 cut(s) 159, 171, 197, 230, 264, 345
Tru9I TTAA 6 cut(s) 159, 171, 197, 230, 264, 345
TscAI CASTG 2 cut(s) 405, 417
TspDTI ATGAA 2 cut(s) 164, 225
TspRI CASTG 2 cut(s) 405, 417
Vha464I CTTAAG 1 cut(s) 263
XapI RAATTY 1 cut(s) 232
XspI CTAG 1 cut(s) 75
Zsp2I ATGCAT 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.