RchiOBHm_Chr3g0493331

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002561
Physical Location & Seq
Reverse (-)
40521346 .. 40522805
1460 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45614

Sequence Viewer

Length: 438 bp
ATGTCGTTGAAAGGCGGCGCTGGATCTTCGCGGGAGGAGCTGAAGAAGATCTTCGACAAGTTCGACAAGAACGGCGACGGCAGGATCTGCTGCGACGAGCTCCGGGCGATCTTCCTTGAGCTCGGCTCCGAAACGACGTCGGAGGAGGTCAAGACCATAATGGCCGAGTTCGACAAGGACGGCGACGGCCGCATTGACCTCGACGAGTTCGCAGAGGTTCTGAACGGCGGATCCACCAAGGATCTCCGCGACGCGTTTGACCTCTACGACCTCGACAAGAACGGCCGCATCTCCGCGAAGGAACTGCACGAGGTTCTCAAGCGATTGGGCCAGAAGTGCTCGCTCAAGGACTGCGAGAAGATGATCCGCTCCGTCGACGCCGACGGCGATCGCCACGTCAACTTCGAGGAATTCAAGAAGATGATGAAGAAACCCTAA

Protein Analysis

145

Amino Acids

16.4

Weight (kDa)

5.1

Isoelectric Point (pI)

33.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 11 - 73 2.4e-18 EF-hand domain pair
EF-hand_1 PF00036 13 - 38 1.1e-07 EF hand domain
EF-hand_6 PF13405 13 - 42 6.4e-08 EF-hand domain
EF-hand_5 PF13202 14 - 38 1.9e-06 EF hand
EF-hand_8 PF13833 33 - 75 5.6e-07 EF-hand domain pair
EF-hand_1 PF00036 49 - 74 3.9e-08 EF hand domain
EF-hand_7 PF13499 80 - 143 5.4e-16 EF-hand domain pair
EF-hand_1 PF00036 81 - 109 3.5e-07 EF hand domain
EF-hand_6 PF13405 81 - 110 4.5e-08 EF-hand domain
EF-hand_5 PF13202 82 - 105 4.3e-06 EF hand
EF-hand_9 PF14658 85 - 143 5.5e-06 EF-hand domain
EF-hand_1 PF00036 117 - 144 1.4e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 140
AccBSI CCGCTC 1 cut(s) 369
AccI GTMKAC 1 cut(s) 375
AccII CGCG 4 cut(s) 31, 249, 254, 296
AciI CCGC 8 cut(s) 15, 31, 190, 228, 247, 286, 294, 367
AclWI GGATC 6 cut(s) 31, 92, 225, 238, 249, 358
AcoI YGGCCR 3 cut(s) 162, 187, 283
AcsI RAATTY 1 cut(s) 410
AcuI CTGAAG 1 cut(s) 62
AcyI GRCGYC 2 cut(s) 137, 378
AflIII ACRYGT 1 cut(s) 252
AgsI TTSAA 2 cut(s) 10, 415
AjiI CACGTC 1 cut(s) 397
AloI GAACNNNNNNTCC 2 cut(s) 215, 247
AluBI AGCT 3 cut(s) 40, 100, 121
AluI AGCT 3 cut(s) 40, 100, 121
Alw21I GWGCWC 3 cut(s) 102, 123, 341
AlwI GGATC 6 cut(s) 31, 92, 225, 238, 249, 358
AlwNI CAGNNNCTG 1 cut(s) 87
AoxI GGCC 4 cut(s) 162, 187, 283, 328
ApeKI GCWGC 1 cut(s) 90
ApoI RAATTY 1 cut(s) 410
AsiSI GCGATCGC 1 cut(s) 391
Asp700I GAANNNNTTC 1 cut(s) 50
AspLEI GCGC 1 cut(s) 20
AspS9I GGNCC 1 cut(s) 328
AsuC2I CCSGG 1 cut(s) 104
BamHI GGATCC 1 cut(s) 230
BanII GRGCYC 2 cut(s) 102, 123
BauI CACGAG 1 cut(s) 308
Bbv12I GWGCWC 3 cut(s) 102, 123, 341
BbvI GCAGC 1 cut(s) 77
BceAI ACGGC 7 cut(s) 88, 94, 196, 202, 241, 298, 400
BcgI CGANNNNNNTGC 4 cut(s) 181, 215, 286, 320
BcnI CCSGG 1 cut(s) 104
BfoI RGCGCY 1 cut(s) 21
BglII AGATCT 1 cut(s) 48
BisI GCNGC 4 cut(s) 16, 91, 190, 286
BlsI GCNGC 4 cut(s) 17, 92, 191, 287
Bme1390I CCNGG 1 cut(s) 104
BmgBI CACGTC 1 cut(s) 397
BmgT120I GGNCC 1 cut(s) 328
BmiI GGNNCC 2 cut(s) 127, 232
BmrFI CCNGG 1 cut(s) 104
BmsI GCATC 1 cut(s) 297
BplI GAGNNNNNCTC 2 cut(s) 110, 142
BpuEI CTTGAG 3 cut(s) 137, 302, 329
BpuMI CCSGG 1 cut(s) 104
BsaHI GRCGYC 2 cut(s) 137, 378
BsaJI CCNNGG 1 cut(s) 237
BseDI CCNNGG 1 cut(s) 237
BseRI GAGGAG 2 cut(s) 50, 158
BseX3I CGGCCG 2 cut(s) 187, 283
BseXI GCAGC 1 cut(s) 77
BsgI GTGCAG 1 cut(s) 290
Bsh1236I CGCG 4 cut(s) 31, 249, 254, 296
Bsh1285I CGRYCG 3 cut(s) 190, 286, 391
BshFI GGCC 4 cut(s) 164, 189, 285, 330
BsiEI CGRYCG 3 cut(s) 190, 286, 391
BsiHKAI GWGCWC 3 cut(s) 102, 123, 341
BsiSI CCGG 1 cut(s) 103
BsnI GGCC 4 cut(s) 164, 189, 285, 330
Bsp1286I GDGCHC 3 cut(s) 102, 123, 341
Bsp143I GATC 8 cut(s) 23, 48, 84, 108, 230, 241, 363, 388
BspACI CCGC 8 cut(s) 15, 31, 190, 228, 247, 286, 294, 367
BspANI GGCC 4 cut(s) 164, 189, 285, 330
BspFNI CGCG 4 cut(s) 31, 249, 254, 296
BspLI GGNNCC 2 cut(s) 127, 232
BspPI GGATC 6 cut(s) 31, 92, 225, 238, 249, 358
BsrBI CCGCTC 1 cut(s) 369
BssECI CCNNGG 1 cut(s) 237
BssMI GATC 8 cut(s) 23, 48, 84, 108, 230, 241, 363, 388
BssNI GRCGYC 2 cut(s) 137, 378
BssSI CACGAG 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 237
Bst2BI CACGAG 1 cut(s) 308
BstACI GRCGYC 2 cut(s) 137, 378
BstAPI GCANNNNNTGC 1 cut(s) 87
BstC8I GCNNGC 1 cut(s) 341
BstFNI CGCG 4 cut(s) 31, 249, 254, 296
BstH2I RGCGCY 1 cut(s) 21
BstHHI GCGC 1 cut(s) 20
BstKTI GATC 8 cut(s) 26, 51, 87, 111, 233, 244, 366, 391
BstMBI GATC 8 cut(s) 23, 48, 84, 108, 230, 241, 363, 388
BstMCI CGRYCG 3 cut(s) 190, 286, 391
BstMWI GCNNNNNNNGC 4 cut(s) 37, 87, 189, 336
BstSCI CCNGG 1 cut(s) 102
BstUI CGCG 4 cut(s) 31, 249, 254, 296
BstV1I GCAGC 1 cut(s) 77
BstX2I RGATCY 5 cut(s) 23, 48, 84, 230, 241
BstYI RGATCY 5 cut(s) 23, 48, 84, 230, 241
BstZI CGGCCG 2 cut(s) 187, 283
BsuRI GGCC 4 cut(s) 164, 189, 285, 330
BtrI CACGTC 1 cut(s) 397
Cac8I GCNNGC 1 cut(s) 341
CaiI CAGNNNCTG 1 cut(s) 87
CfoI GCGC 1 cut(s) 20
Cfr13I GGNCC 1 cut(s) 328
CseI GACGC 2 cut(s) 260, 386
CviJI RGCY 8 cut(s) 40, 100, 121, 126, 164, 189, 285, 330
CviKI_1 RGCY 8 cut(s) 40, 100, 121, 126, 164, 189, 285, 330
DpnI GATC 8 cut(s) 25, 50, 86, 110, 232, 243, 365, 390
DpnII GATC 8 cut(s) 23, 48, 84, 108, 230, 241, 363, 388
EaeI YGGCCR 3 cut(s) 162, 187, 283
EagI CGGCCG 2 cut(s) 187, 283
EciI GGCGGA 1 cut(s) 243
Ecl136II GAGCTC 2 cut(s) 100, 121
EclXI CGGCCG 2 cut(s) 187, 283
Eco130I CCWWGG 1 cut(s) 237
Eco24I GRGCYC 2 cut(s) 102, 123
Eco52I CGGCCG 2 cut(s) 187, 283
Eco53kI GAGCTC 2 cut(s) 100, 121
Eco57I CTGAAG 1 cut(s) 62
EcoICRI GAGCTC 2 cut(s) 100, 121
EcoRI GAATTC 1 cut(s) 410
EcoT14I CCWWGG 1 cut(s) 237
EcoT38I GRGCYC 2 cut(s) 102, 123
ErhI CCWWGG 1 cut(s) 237
FaiI YATR 1 cut(s) 158
FalI AAGNNNNNCTT 2 cut(s) 35, 67
FauI CCCGC 1 cut(s) 24
FblI GTMKAC 1 cut(s) 375
Fnu4HI GCNGC 4 cut(s) 16, 91, 190, 286
FriOI GRGCYC 2 cut(s) 102, 123
Fsp4HI GCNGC 4 cut(s) 16, 91, 190, 286
GlaI GCGC 1 cut(s) 19
GluI GCNGC 4 cut(s) 16, 91, 190, 286
HaeII RGCGCY 1 cut(s) 21
HaeIII GGCC 4 cut(s) 164, 189, 285, 330
HapII CCGG 1 cut(s) 103
HgaI GACGC 2 cut(s) 260, 386
HhaI GCGC 1 cut(s) 20
Hin1I GRCGYC 2 cut(s) 137, 378
Hin6I GCGC 1 cut(s) 18
HinP1I GCGC 1 cut(s) 18
HincII GTYRAC 2 cut(s) 376, 400
HindII GTYRAC 2 cut(s) 376, 400
HpaII CCGG 1 cut(s) 103
Hpy166II GTNNAC 2 cut(s) 376, 400
Hpy188I TCNGA 3 cut(s) 130, 142, 222
Hpy188III TCNNGA 2 cut(s) 151, 415
Hpy8I GTNNAC 2 cut(s) 376, 400
HpyAV CCTTC 1 cut(s) 292
HpyCH4IV ACGT 2 cut(s) 137, 396
HpyCH4V TGCA 1 cut(s) 307
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 87, 189, 336
HpySE526I ACGT 2 cut(s) 137, 396
Hsp92I GRCGYC 2 cut(s) 137, 378
HspAI GCGC 1 cut(s) 18
Kzo9I GATC 8 cut(s) 23, 48, 84, 108, 230, 241, 363, 388
LmnI GCTCC 4 cut(s) 37, 105, 131, 374
LpnPI CCDG 4 cut(s) 6, 67, 116, 344
Lsp1109I GCAGC 1 cut(s) 77
LweI GCATC 1 cut(s) 297
MaeII ACGT 2 cut(s) 137, 396
MalI GATC 8 cut(s) 25, 50, 86, 110, 232, 243, 365, 390
MbiI CCGCTC 1 cut(s) 369
MboI GATC 8 cut(s) 23, 48, 84, 108, 230, 241, 363, 388
MboII GAAGA 7 cut(s) 18, 43, 55, 58, 103, 370, 430
MflI RGATCY 5 cut(s) 23, 48, 84, 230, 241
MhlI GDGCHC 3 cut(s) 102, 123, 341
MluCI AATT 1 cut(s) 410
MluI ACGCGT 1 cut(s) 252
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 9 cut(s) 28, 136, 139, 208, 209, 272, 281, 304, 400
MroXI GAANNNNTTC 1 cut(s) 50
MspI CCGG 1 cut(s) 103
MspR9I CCNGG 1 cut(s) 104
MvnI CGCG 4 cut(s) 31, 249, 254, 296
MwoI GCNNNNNNNGC 4 cut(s) 37, 87, 189, 336
NciI CCSGG 1 cut(s) 104
NdeII GATC 8 cut(s) 23, 48, 84, 108, 230, 241, 363, 388
NlaIV GGNNCC 2 cut(s) 127, 232
NmeAIII GCCGAG 2 cut(s) 102, 190
PcsI WCGNNNNNNNCGW 3 cut(s) 60, 384, 402
PdmI GAANNNNTTC 1 cut(s) 50
PkrI GCNGC 4 cut(s) 17, 92, 191, 287
Ple19I CGATCG 1 cut(s) 391
Psp124BI GAGCTC 2 cut(s) 102, 123
PspN4I GGNNCC 2 cut(s) 127, 232
PspPI GGNCC 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 87
PsuI RGATCY 5 cut(s) 23, 48, 84, 230, 241
PvuI CGATCG 1 cut(s) 391
RgaI GCGATCGC 1 cut(s) 391
SacI GAGCTC 2 cut(s) 102, 123
SalI GTCGAC 1 cut(s) 374
SatI GCNGC 4 cut(s) 16, 91, 190, 286
Sau3AI GATC 8 cut(s) 23, 48, 84, 108, 230, 241, 363, 388
Sau96I GGNCC 1 cut(s) 328
ScrFI CCNGG 1 cut(s) 104
SduI GDGCHC 3 cut(s) 102, 123, 341
SfaAI GCGATCGC 1 cut(s) 391
SfaNI GCATC 1 cut(s) 297
SgfI GCGATCGC 1 cut(s) 391
SgrDI CGTCGACG 1 cut(s) 374
SmlI CTYRAG 3 cut(s) 116, 317, 344
SmoI CTYRAG 3 cut(s) 116, 317, 344
Sse9I AATT 1 cut(s) 410
SsiI CCGC 8 cut(s) 15, 31, 190, 228, 247, 286, 294, 367
SstI GAGCTC 2 cut(s) 102, 123
StyD4I CCNGG 1 cut(s) 102
StyI CCWWGG 1 cut(s) 237
TaiI ACGT 2 cut(s) 140, 399
TaqI TCGA 7 cut(s) 54, 63, 171, 201, 273, 375, 405
TasI AATT 1 cut(s) 410
TauI GCSGC 3 cut(s) 18, 192, 288
TseI GCWGC 1 cut(s) 90
TspGWI ACGGA 1 cut(s) 361
XapI RAATTY 1 cut(s) 410
XmiI GTMKAC 1 cut(s) 375
XmnI GAANNNNTTC 1 cut(s) 50
ZraI GACGTC 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.