Rh1CG143900

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0002561
Physical Location & Seq
Reverse (-)
31074592 .. 31104974
30383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG143900.1

Sequence Viewer

Length: 552 bp
ATGGCTGAACACTGGGATATCTACCCTCTCACACAATCCAATCCTAACAAGCAAGAAGAAACCAAATCAAATTTGTACTCCCTCTCAGCGATATCATCGAGTTTCAACAGTTCAAAAAGCGGCAGCAGCGTAGTGGTACCTATGGAAGAGGTACAGAAGGTCTTCAACAAGTTCGATGAGAACAGTGACGGACTCATCTCCTGTGATGAGCTCAAGAACGTTTTCTGTAAGTTGGGGTGGGAGATGAAGCCAGAGGAAGTGCAGACTGTGATTTCTGAGTTCGACAAGGACGGGGACGGCCACATCGACCTGGAGGAGTTCACAAAAATCATGAATGAATTGGGGTCGAGCAAGGATCTCCTTGACGCCTTCCGTGTGTACGATCTAGACAAGAATGGCCTGATCTCGGCGAGCGAACTTCATGAGGTGCTCAACCACTTAGGGGAGAAGTGCTCGGTTGGGGAATGTGCCACCATGATCACCAAATTTGACGTCAATGGTGATGGCTTCATCAACTTTGAAGAGTTCAAGCAGATGATGAAGCGCTCTTGA

Protein Analysis

183

Amino Acids

20.66

Weight (kDa)

4.56

Isoelectric Point (pI)

44.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 49 - 111 1.2e-14 EF-hand domain pair
EF-hand_1 PF00036 50 - 75 9.2e-06 EF hand domain
EF-hand_6 PF13405 51 - 79 4.1e-06 EF-hand domain
EF-hand_8 PF13833 78 - 111 6.1e-07 EF-hand domain pair
EF-hand_1 PF00036 86 - 113 3e-08 EF hand domain
EF-hand_5 PF13202 89 - 111 9.6e-06 EF hand
EF-hand_7 PF13499 118 - 181 4.1e-15 EF-hand domain pair
EF-hand_1 PF00036 120 - 147 8e-06 EF hand domain
EF-hand_6 PF13405 120 - 148 2.5e-06 EF-hand domain
EF-hand_8 PF13833 145 - 182 5.3e-07 EF-hand domain pair
EF-hand_1 PF00036 156 - 182 2.1e-09 EF hand domain
EF-hand_5 PF13202 162 - 180 1.5e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 495
Acc65I GGTACC 1 cut(s) 136
AccB1I GGYRCC 1 cut(s) 136
AciI CCGC 1 cut(s) 120
AclI AACGTT 1 cut(s) 219
AclWI GGATC 1 cut(s) 363
AcoI YGGCCR 1 cut(s) 298
AcsI RAATTY 2 cut(s) 70, 485
AcyI GRCGYC 2 cut(s) 366, 492
AfaI GTAC 4 cut(s) 77, 138, 153, 380
AfeI AGCGCT 1 cut(s) 545
AfiI CCNNNNNNNGG 2 cut(s) 406, 442
AgsI TTSAA 5 cut(s) 106, 114, 166, 521, 529
AjnI CCWGG 1 cut(s) 309
AluBI AGCT 1 cut(s) 211
AluI AGCT 1 cut(s) 211
Alw21I GWGCWC 3 cut(s) 213, 432, 455
AlwI GGATC 1 cut(s) 363
Aor51HI AGCGCT 1 cut(s) 545
AoxI GGCC 2 cut(s) 298, 397
ApeKI GCWGC 2 cut(s) 123, 126
ApoI RAATTY 2 cut(s) 70, 485
ArsI GACNNNNNNTTYG 2 cut(s) 477, 509
Asp700I GAANNNNTTC 2 cut(s) 161, 221
Asp718I GGTACC 1 cut(s) 136
AspLEI GCGC 1 cut(s) 546
AsuHPI GGTGA 2 cut(s) 472, 512
BanI GGYRCC 1 cut(s) 136
BanII GRGCYC 1 cut(s) 213
BbsI GAAGAC 1 cut(s) 154
Bbv12I GWGCWC 3 cut(s) 213, 432, 455
BbvI GCAGC 2 cut(s) 135, 138
BccI CCATC 1 cut(s) 497
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 1 cut(s) 311
BclI TGATCA 1 cut(s) 477
BfaI CTAG 1 cut(s) 386
BfoI RGCGCY 1 cut(s) 547
BisI GCNGC 3 cut(s) 121, 124, 127
BlsI GCNGC 3 cut(s) 122, 125, 128
Bme1390I CCNGG 1 cut(s) 311
BmiI GGNNCC 1 cut(s) 138
BmrFI CCNGG 1 cut(s) 311
BmrI ACTGGG 1 cut(s) 22
BmuI ACTGGG 1 cut(s) 22
BpiI GAAGAC 1 cut(s) 154
BplI GAGNNNNNCTC 2 cut(s) 437, 469
BpmI CTGGAG 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 197
BsaHI GRCGYC 2 cut(s) 366, 492
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 442
Bse1I ACTGG 1 cut(s) 17
BseBI CCWGG 1 cut(s) 311
BseLI CCNNNNNNNGG 2 cut(s) 406, 442
BseMII CTCAG 2 cut(s) 99, 267
BseNI ACTGG 1 cut(s) 17
BseRI GAGGAG 1 cut(s) 329
BseXI GCAGC 2 cut(s) 135, 138
BsgI GTGCAG 1 cut(s) 281
BshFI GGCC 2 cut(s) 300, 399
BshNI GGYRCC 1 cut(s) 136
BsiHKAI GWGCWC 3 cut(s) 213, 432, 455
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 2 cut(s) 406, 442
BsmFI GGGAC 1 cut(s) 308
BsnI GGCC 2 cut(s) 300, 399
Bsp1286I GDGCHC 3 cut(s) 213, 432, 455
Bsp143I GATC 4 cut(s) 355, 382, 402, 477
BspACI CCGC 1 cut(s) 120
BspANI GGCC 2 cut(s) 300, 399
BspCNI CTCAG 2 cut(s) 98, 268
BspHI TCATGA 2 cut(s) 330, 421
BspLI GGNNCC 1 cut(s) 138
BspPI GGATC 1 cut(s) 363
BspT107I GGYRCC 1 cut(s) 136
BsrI ACTGG 1 cut(s) 17
BssMI GATC 4 cut(s) 355, 382, 402, 477
BssNI GRCGYC 2 cut(s) 366, 492
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 3 cut(s) 110, 185, 268
Bst6I CTCTTC 2 cut(s) 141, 516
BstACI GRCGYC 2 cut(s) 366, 492
BstC8I GCNNGC 1 cut(s) 412
BstDEI CTNAG 3 cut(s) 85, 276, 439
BstH2I RGCGCY 1 cut(s) 547
BstHHI GCGC 1 cut(s) 546
BstKTI GATC 4 cut(s) 358, 385, 405, 480
BstMBI GATC 4 cut(s) 355, 382, 402, 477
BstMWI GCNNNNNNNGC 1 cut(s) 126
BstNI CCWGG 1 cut(s) 311
BstSCI CCNGG 1 cut(s) 309
BstV1I GCAGC 2 cut(s) 135, 138
BstV2I GAAGAC 1 cut(s) 154
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
BsuRI GGCC 2 cut(s) 300, 399
BtsIMutI CAGTG 2 cut(s) 10, 190
Cac8I GCNNGC 1 cut(s) 412
CciI TCATGA 2 cut(s) 330, 421
CfoI GCGC 1 cut(s) 546
CseI GACGC 1 cut(s) 374
Csp6I GTAC 4 cut(s) 76, 137, 152, 379
CviAII CATG 3 cut(s) 331, 422, 475
CviJI RGCY 6 cut(s) 5, 211, 250, 300, 399, 507
CviKI_1 RGCY 6 cut(s) 5, 211, 250, 300, 399, 507
CviQI GTAC 4 cut(s) 76, 137, 152, 379
DdeI CTNAG 3 cut(s) 85, 276, 439
DpnI GATC 4 cut(s) 357, 384, 404, 479
DpnII GATC 4 cut(s) 355, 382, 402, 477
EaeI YGGCCR 1 cut(s) 298
Eam1104I CTCTTC 2 cut(s) 141, 516
EarI CTCTTC 2 cut(s) 141, 516
Ecl136II GAGCTC 1 cut(s) 211
Eco24I GRGCYC 1 cut(s) 213
Eco32I GATATC 2 cut(s) 19, 93
Eco47III AGCGCT 1 cut(s) 545
Eco53kI GAGCTC 1 cut(s) 211
EcoICRI GAGCTC 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 309
EcoRV GATATC 2 cut(s) 19, 93
EcoT38I GRGCYC 1 cut(s) 213
FaeI CATG 3 cut(s) 334, 425, 478
FaiI YATR 4 cut(s) 143, 332, 423, 476
FaqI GGGAC 1 cut(s) 308
FatI CATG 3 cut(s) 330, 421, 474
FbaI TGATCA 1 cut(s) 477
Fnu4HI GCNGC 3 cut(s) 121, 124, 127
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 3 cut(s) 121, 124, 127
FspBI CTAG 1 cut(s) 386
GlaI GCGC 1 cut(s) 545
GluI GCNGC 3 cut(s) 121, 124, 127
GsuI CTGGAG 1 cut(s) 332
HaeII RGCGCY 1 cut(s) 547
HaeIII GGCC 2 cut(s) 300, 399
HgaI GACGC 1 cut(s) 374
HhaI GCGC 1 cut(s) 546
Hin1I GRCGYC 2 cut(s) 366, 492
Hin1II CATG 3 cut(s) 334, 425, 478
Hin6I GCGC 1 cut(s) 544
HinP1I GCGC 1 cut(s) 544
HinfI GANTC 1 cut(s) 192
HphI GGTGA 2 cut(s) 472, 512
Hpy166II GTNNAC 2 cut(s) 321, 379
Hpy188I TCNGA 1 cut(s) 277
Hpy188III TCNNGA 5 cut(s) 214, 331, 386, 422, 549
Hpy8I GTNNAC 2 cut(s) 321, 379
HpyAV CCTTC 2 cut(s) 151, 379
HpyCH4III ACNGT 3 cut(s) 110, 185, 268
HpyCH4IV ACGT 2 cut(s) 219, 492
HpyCH4V TGCA 1 cut(s) 262
HpyF10VI GCNNNNNNNGC 1 cut(s) 126
HpyF3I CTNAG 3 cut(s) 85, 276, 439
HpySE526I ACGT 2 cut(s) 219, 492
Hsp92I GRCGYC 2 cut(s) 366, 492
Hsp92II CATG 3 cut(s) 334, 425, 478
HspAI GCGC 1 cut(s) 544
KpnI GGTACC 1 cut(s) 140
Ksp22I TGATCA 1 cut(s) 477
Kzo9I GATC 4 cut(s) 355, 382, 402, 477
LpnPI CCDG 5 cut(s) 214, 264, 296, 323, 413
Lsp1109I GCAGC 2 cut(s) 135, 138
MaeI CTAG 1 cut(s) 386
MaeII ACGT 2 cut(s) 219, 492
MaeIII GTNAC 1 cut(s) 185
MalI GATC 4 cut(s) 357, 384, 404, 479
MboI GATC 4 cut(s) 355, 382, 402, 477
MboII GAAGA 4 cut(s) 68, 154, 158, 533
MflI RGATCY 1 cut(s) 355
MhlI GDGCHC 3 cut(s) 213, 432, 455
MluCI AATT 3 cut(s) 70, 338, 485
MlyI GAGTC 1 cut(s) 186
MnlI CCTC 6 cut(s) 36, 92, 142, 247, 307, 418
MroXI GAANNNNTTC 2 cut(s) 161, 221
MspR9I CCNGG 1 cut(s) 311
MvaI CCWGG 1 cut(s) 311
MwoI GCNNNNNNNGC 1 cut(s) 126
NdeII GATC 4 cut(s) 355, 382, 402, 477
NlaIII CATG 3 cut(s) 334, 425, 478
NlaIV GGNNCC 1 cut(s) 138
NmeAIII GCCGAG 1 cut(s) 386
NmuCI GTSAC 1 cut(s) 185
PagI TCATGA 2 cut(s) 330, 421
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PdmI GAANNNNTTC 2 cut(s) 161, 221
PkrI GCNGC 3 cut(s) 122, 125, 128
PleI GAGTC 1 cut(s) 186
PpsI GAGTC 1 cut(s) 186
Psp124BI GAGCTC 1 cut(s) 213
Psp1406I AACGTT 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 309
PspGI CCWGG 1 cut(s) 309
PspN4I GGNNCC 1 cut(s) 138
PsuI RGATCY 1 cut(s) 355
RsaI GTAC 4 cut(s) 77, 138, 153, 380
RsaNI GTAC 4 cut(s) 76, 137, 152, 379
SacI GAGCTC 1 cut(s) 213
SatI GCNGC 3 cut(s) 121, 124, 127
Sau3AI GATC 4 cut(s) 355, 382, 402, 477
SchI GAGTC 1 cut(s) 186
ScrFI CCNGG 1 cut(s) 311
SduI GDGCHC 3 cut(s) 213, 432, 455
SetI ASST 8 cut(s) 142, 153, 162, 213, 222, 312, 429, 495
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
Sse9I AATT 3 cut(s) 70, 338, 485
SsiI CCGC 1 cut(s) 120
SspMI CTAG 1 cut(s) 386
SstI GAGCTC 1 cut(s) 213
StyD4I CCNGG 1 cut(s) 309
TaaI ACNGT 3 cut(s) 110, 185, 268
TaiI ACGT 2 cut(s) 222, 495
TaqI TCGA 5 cut(s) 98, 174, 282, 306, 347
TasI AATT 3 cut(s) 70, 338, 485
TatI WGTACW 1 cut(s) 75
TauI GCSGC 1 cut(s) 123
TscAI CASTG 2 cut(s) 17, 190
TseFI GTSAC 1 cut(s) 185
TseI GCWGC 2 cut(s) 123, 126
Tsp45I GTSAC 1 cut(s) 185
TspDTI ATGAA 5 cut(s) 260, 347, 351, 410, 499
TspGWI ACGGA 2 cut(s) 204, 362
TspRI CASTG 2 cut(s) 17, 190
XapI RAATTY 2 cut(s) 70, 485
XbaI TCTAGA 1 cut(s) 385
XmnI GAANNNNTTC 2 cut(s) 161, 221
XspI CTAG 1 cut(s) 386
ZraI GACGTC 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.