Rroxscaffold_4G00314650

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002561
Physical Location & Seq
Forward (+)
38648915 .. 38649376
462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00314650.1

Sequence Viewer

Length: 462 bp
ATGTCATCGAGTTTCAGCAGTTCAAGAAGTGGCAGCGGCATGGTGGTACCCATGGGAGAGGTACAGAAGGTCTTCAACAAGTTCGACGAGAATGGTGACGGACTCATCTCTTGCGATGAGCTCAAGAACGTTTTGTTTGAGCTGGGGTGCGAGATGAAGTCGGAGGCAGTGCAGACTGTGATGTCCGAGTTCGACAAGGACGGCGACGGCCACATCGACCTGGAGGAGTTCAAAAAACTCATGAACGAATCTGCGTCAAGCGAGGATATCCGTAACGCCTTCGATGTCTACGATCTCGACAAGAACGGCCTGATCTCGGCTAGCGAACTTCATGAGGTGCTGAACCGCTTAGGGCTGAAGTGCTCCATCCGGGAATGTGCCACCATGATCACCAAATTTGACGCCAATGGAGATGGCTTCATTAACTTTCAAGAGTTCAAGAATATGTTGAACCGCTCTTAA

Protein Analysis

153

Amino Acids

17.03

Weight (kDa)

4.53

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 20 - 45 8.7e-07 EF hand domain
EF-hand_6 PF13405 21 - 49 6.4e-07 EF-hand domain
EF-hand_7 PF13499 22 - 81 8.2e-17 EF-hand domain pair
EF-hand_8 PF13833 52 - 82 5.6e-07 EF-hand domain pair
EF-hand_1 PF00036 58 - 82 1.3e-08 EF hand domain
EF-hand_5 PF13202 59 - 81 6.9e-07 EF hand
EF-hand_7 PF13499 88 - 149 5.4e-14 EF-hand domain pair
EF-hand_1 PF00036 89 - 116 4.4e-06 EF hand domain
EF-hand_6 PF13405 89 - 118 1.4e-06 EF-hand domain
EF-hand_1 PF00036 126 - 152 3.3e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 181
Acc65I GGTACC 1 cut(s) 46
AccB1I GGYRCC 1 cut(s) 46
AccBSI CCGCTC 1 cut(s) 456
AccI GTMKAC 1 cut(s) 288
AciI CCGC 3 cut(s) 36, 346, 454
AclI AACGTT 1 cut(s) 129
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 1 cut(s) 395
AcuI CTGAAG 1 cut(s) 377
AcyI GRCGYC 1 cut(s) 402
AfaI GTAC 2 cut(s) 48, 63
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 6 cut(s) 24, 76, 232, 431, 439, 451
AjnI CCWGG 1 cut(s) 219
AluBI AGCT 2 cut(s) 121, 142
AluI AGCT 2 cut(s) 121, 142
Alw21I GWGCWC 2 cut(s) 123, 365
AoxI GGCC 2 cut(s) 208, 307
ApeKI GCWGC 1 cut(s) 33
ApoI RAATTY 1 cut(s) 395
Asp700I GAANNNNTTC 1 cut(s) 71
Asp718I GGTACC 1 cut(s) 46
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 2 cut(s) 107, 382
AsuNHI GCTAGC 1 cut(s) 320
BanI GGYRCC 1 cut(s) 46
BanII GRGCYC 1 cut(s) 123
BbsI GAAGAC 1 cut(s) 64
Bbv12I GWGCWC 2 cut(s) 123, 365
BbvI GCAGC 1 cut(s) 45
BccI CCATC 2 cut(s) 374, 407
BceAI ACGGC 3 cut(s) 217, 223, 322
BciT130I CCWGG 1 cut(s) 221
BclI TGATCA 1 cut(s) 387
BcnI CCSGG 1 cut(s) 371
BfaI CTAG 1 cut(s) 321
BisI GCNGC 2 cut(s) 34, 37
BlsI GCNGC 2 cut(s) 35, 38
Bme1390I CCNGG 2 cut(s) 221, 371
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 2 cut(s) 221, 371
BmtI GCTAGC 1 cut(s) 324
BpiI GAAGAC 1 cut(s) 64
BpmI CTGGAG 1 cut(s) 242
Bpu10I CCTNAGC 1 cut(s) 349
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 1 cut(s) 371
BsaHI GRCGYC 1 cut(s) 402
BsaJI CCNNGG 1 cut(s) 51
Bsc4I CCNNNNNNNGG 1 cut(s) 316
BseBI CCWGG 1 cut(s) 221
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 1 cut(s) 366
BseLI CCNNNNNNNGG 1 cut(s) 316
BseRI GAGGAG 1 cut(s) 239
BseXI GCAGC 1 cut(s) 45
BseYI CCCAGC 1 cut(s) 142
BsgI GTGCAG 1 cut(s) 191
BshFI GGCC 2 cut(s) 210, 309
BshNI GGYRCC 1 cut(s) 46
BsiHKAI GWGCWC 2 cut(s) 123, 365
BsiSI CCGG 1 cut(s) 370
BslI CCNNNNNNNGG 1 cut(s) 316
BsnI GGCC 2 cut(s) 210, 309
Bsp1286I GDGCHC 2 cut(s) 123, 365
Bsp143I GATC 3 cut(s) 292, 312, 387
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 3 cut(s) 36, 346, 454
BspANI GGCC 2 cut(s) 210, 309
BspHI TCATGA 2 cut(s) 240, 331
BspLI GGNNCC 1 cut(s) 48
BspOI GCTAGC 1 cut(s) 324
BspT107I GGYRCC 1 cut(s) 46
BsrBI CCGCTC 1 cut(s) 456
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 3 cut(s) 292, 312, 387
BssNI GRCGYC 1 cut(s) 402
BssT1I CCWWGG 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 1 cut(s) 178
BstACI GRCGYC 1 cut(s) 402
BstC8I GCNNGC 1 cut(s) 322
BstDEI CTNAG 1 cut(s) 349
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 1 cut(s) 366
BstKTI GATC 3 cut(s) 295, 315, 390
BstMBI GATC 3 cut(s) 292, 312, 387
BstNI CCWGG 1 cut(s) 221
BstSCI CCNGG 2 cut(s) 219, 369
BstV1I GCAGC 1 cut(s) 45
BstV2I GAAGAC 1 cut(s) 64
BsuRI GGCC 2 cut(s) 210, 309
BtgI CCRYGG 1 cut(s) 51
BtgZI GCGATG 1 cut(s) 129
BtsCI GGATG 1 cut(s) 366
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 1 cut(s) 174
Cac8I GCNNGC 1 cut(s) 322
CciI TCATGA 2 cut(s) 240, 331
CseI GACGC 2 cut(s) 243, 410
Csp6I GTAC 2 cut(s) 47, 62
CviAII CATG 5 cut(s) 40, 52, 241, 332, 385
CviJI RGCY 7 cut(s) 121, 142, 210, 309, 320, 355, 417
CviKI_1 RGCY 7 cut(s) 121, 142, 210, 309, 320, 355, 417
CviQI GTAC 2 cut(s) 47, 62
DdeI CTNAG 1 cut(s) 349
DpnI GATC 3 cut(s) 294, 314, 389
DpnII GATC 3 cut(s) 292, 312, 387
DrdI GACNNNNNNGTC 1 cut(s) 181
DseDI GACNNNNNNGTC 1 cut(s) 181
EaeI YGGCCR 1 cut(s) 208
Ecl136II GAGCTC 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 123
Eco32I GATATC 1 cut(s) 268
Eco53kI GAGCTC 1 cut(s) 121
Eco57I CTGAAG 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 219
EcoRV GATATC 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 5 cut(s) 43, 55, 244, 335, 388
FaiI YATR 6 cut(s) 41, 53, 242, 333, 386, 446
FatI CATG 5 cut(s) 39, 51, 240, 331, 384
FbaI TGATCA 1 cut(s) 387
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 2 cut(s) 34, 37
FokI GGATG 1 cut(s) 353
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 2 cut(s) 34, 37
FspBI CTAG 1 cut(s) 321
GluI GCNGC 2 cut(s) 34, 37
GsaI CCCAGC 1 cut(s) 146
GsuI CTGGAG 1 cut(s) 242
HaeIII GGCC 2 cut(s) 210, 309
HapII CCGG 1 cut(s) 370
HgaI GACGC 2 cut(s) 243, 410
Hin1I GRCGYC 1 cut(s) 402
Hin1II CATG 5 cut(s) 43, 55, 244, 335, 388
HinfI GANTC 2 cut(s) 102, 248
HpaII CCGG 1 cut(s) 370
HphI GGTGA 2 cut(s) 107, 382
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 2 cut(s) 163, 187
Hpy188III TCNNGA 7 cut(s) 24, 124, 241, 296, 332, 431, 439
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 2 cut(s) 89, 209
HpyAV CCTTC 2 cut(s) 61, 289
HpyCH4III ACNGT 1 cut(s) 178
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 1 cut(s) 172
HpyF3I CTNAG 1 cut(s) 349
HpySE526I ACGT 1 cut(s) 129
Hsp92I GRCGYC 1 cut(s) 402
Hsp92II CATG 5 cut(s) 43, 55, 244, 335, 388
KpnI GGTACC 1 cut(s) 50
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 3 cut(s) 292, 312, 387
LmnI GCTCC 1 cut(s) 368
LpnPI CCDG 5 cut(s) 128, 206, 233, 323, 383
Lsp1109I GCAGC 1 cut(s) 45
MaeI CTAG 1 cut(s) 321
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 2 cut(s) 95, 272
MalI GATC 3 cut(s) 294, 314, 389
MbiI CCGCTC 1 cut(s) 456
MboI GATC 3 cut(s) 292, 312, 387
MboII GAAGA 1 cut(s) 64
MhlI GDGCHC 2 cut(s) 123, 365
MluCI AATT 1 cut(s) 395
MlyI GAGTC 1 cut(s) 96
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 5 cut(s) 52, 157, 217, 256, 328
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 2 cut(s) 423, 460
MspA1I CMGCKG 1 cut(s) 36
MspI CCGG 1 cut(s) 370
MspR9I CCNGG 2 cut(s) 221, 371
MvaI CCWGG 1 cut(s) 221
NciI CCSGG 1 cut(s) 371
NcoI CCATGG 1 cut(s) 51
NdeII GATC 3 cut(s) 292, 312, 387
NheI GCTAGC 1 cut(s) 320
NlaIII CATG 5 cut(s) 43, 55, 244, 335, 388
NlaIV GGNNCC 1 cut(s) 48
NmeAIII GCCGAG 1 cut(s) 296
NmuCI GTSAC 1 cut(s) 95
PagI TCATGA 2 cut(s) 240, 331
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 288
PdmI GAANNNNTTC 1 cut(s) 71
PfeI GAWTC 1 cut(s) 248
PfoI TCCNGGA 1 cut(s) 369
PkrI GCNGC 2 cut(s) 35, 38
PleI GAGTC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 96
Psp124BI GAGCTC 1 cut(s) 123
Psp1406I AACGTT 1 cut(s) 129
Psp6I CCWGG 1 cut(s) 219
PspFI CCCAGC 1 cut(s) 142
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 1 cut(s) 48
RsaI GTAC 2 cut(s) 48, 63
RsaNI GTAC 2 cut(s) 47, 62
SacI GAGCTC 1 cut(s) 123
SaqAI TTAA 2 cut(s) 423, 460
SatI GCNGC 2 cut(s) 34, 37
Sau3AI GATC 3 cut(s) 292, 312, 387
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 2 cut(s) 221, 371
SduI GDGCHC 2 cut(s) 123, 365
SetI ASST 7 cut(s) 63, 72, 123, 132, 144, 222, 339
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 1 cut(s) 395
SsiI CCGC 3 cut(s) 36, 346, 454
SspMI CTAG 1 cut(s) 321
SstI GAGCTC 1 cut(s) 123
StyD4I CCNGG 2 cut(s) 219, 369
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 178
TaiI ACGT 1 cut(s) 132
TaqI TCGA 6 cut(s) 8, 84, 192, 216, 282, 297
TasI AATT 1 cut(s) 395
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 1 cut(s) 248
Tru1I TTAA 2 cut(s) 423, 460
Tru9I TTAA 2 cut(s) 423, 460
TscAI CASTG 1 cut(s) 174
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 1 cut(s) 33
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 4 cut(s) 170, 257, 320, 409
TspGWI ACGGA 2 cut(s) 114, 260
TspRI CASTG 1 cut(s) 174
XapI RAATTY 1 cut(s) 395
XmiI GTMKAC 1 cut(s) 288
XmnI GAANNNNTTC 1 cut(s) 71
XspI CTAG 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.