RchiOBHm_Chr1g0369221

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003324
Physical Location & Seq
Forward (+)
59110661 .. 59114185
3525 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59347

Sequence Viewer

Length: 1260 bp
ATGTCGAAATCGTCCTCTTCCCTCTTCGACCTTCTCCTCCAGTTATCGGAGCCGATTGCCGAATCTCTGAGCAAAACGCCGTACACGCCGTCACAGACCGCCAACGTCTCCGTGAAAGCCTTACTGGAACCCCTCCTTCCTCGAAACCCTAATCCTAACATCGGCCTCCGCGCCTCAATCCGAGACTTCACTCTCGCCTGTGCTCTACTCTCCTCCTCCCAATCCTCAACCCACGAGCTTCTCTCGTGGATCCCGGAACACCTCGCGGCCGCTGCTGACCGGGCGGTTCTGGAATTCTCGAAAGCTTATTCCGATTACGGCGAGAAAGCTGGTGATGGTTTGGTGGCGGAGTTGATGCCTGAGGTGATGCCGTTACTGAAGGAGAGGATCAAGGAGAGCTCGATTGATAAATCGGCGGACGGGGATGAGGTCTCGGCGGCGTCGGCAAGAGTGCCGGTTGGGTTTGCCATTGTGGCTGCTTATCAGTTTAGGTGGTTTGTTACACAGATTGATTATTCGGATTTTGGAAAGTTGTGTGCTTTGGCTGTTCCTTGCGCTTTGACTGCTCTGGATCACTGGTCGCCGGAGATTAAAGGGCAAGGCATGATAAGCTTTATACATATCGCCAAAAGTGTAGATGCTGCTGAACTAACTTGGTGTCAAGATGCAATTCTTGATGCATGCTGCCAGAATATTGCTTCTAGTGACGAGATATGGCATCTTGTGGTGGAGATGTCAGTACTTGTGGTGACTTGTACCCAGCAGAGTAATCCTCGTAGTGCATGGTTTGATAAAATTCTAAATGAGATGTTAACTCACTTGGAGCGCCAGCCTAGGAACAAGGAGCGCCGTATTGCATGGCTTCGTTATGTAGAGCCGCTTTTTAATGGTGTTGGTCTGGTGTTACTAGCTCATTTCAGGCGCATTTTCCCTCTTTTCTTTAAGTGGATGCATGCTGATGACGATGAGACTGTTTTACTGGTTCTCAAACAGATTGAAACAGTTATAAAGCTAACATGGATTAGGAATACACCATATGTGGAAAGGTTGGTGGATGAACTTGCTACTTTGCACAAAGAAGCAGCAATGAAAAGATCTCGTGAAGAAATTAGAAATCTTGTTATTCGTATACTGATCCTGCTCCATCAATGCAAGGGTCTGCAGTTCGAAGCAGCCTGGGGCAAGCACAGGGATGATCCAAACTTGGCAACAATTGCTCCCTCTTTAAGTGAAAGAAGATCAACAATGGCTTTCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

419

Amino Acids

47.07

Weight (kDa)

5.92

Isoelectric Point (pI)

50.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1007
AccI GTMKAC 1 cut(s) 1129
AccII CGCG 2 cut(s) 171, 266
AciI CCGC 9 cut(s) 99, 169, 266, 270, 284, 347, 416, 437, 878
AclWI GGATC 6 cut(s) 244, 257, 395, 579, 1129, 1190
AcoI YGGCCR 1 cut(s) 267
AcsI RAATTY 2 cut(s) 293, 795
AcuI CTGAAG 1 cut(s) 398
AcyI GRCGYC 1 cut(s) 440
AfaI GTAC 3 cut(s) 83, 741, 757
AfiI CCNNNNNNNGG 2 cut(s) 46, 161
AgsI TTSAA 1 cut(s) 998
AjnI CCWGG 1 cut(s) 1175
AluBI AGCT 7 cut(s) 238, 305, 329, 399, 612, 911, 1012
AluI AGCT 7 cut(s) 238, 305, 329, 399, 612, 911, 1012
Alw21I GWGCWC 2 cut(s) 205, 401
Alw26I GTCTC 4 cut(s) 112, 177, 436, 962
AlwI GGATC 6 cut(s) 244, 257, 395, 579, 1129, 1190
AoxI GGCC 2 cut(s) 163, 267
ApeKI GCWGC 6 cut(s) 272, 476, 641, 684, 1082, 1172
ApoI RAATTY 2 cut(s) 293, 795
AspA2I CCTAGG 1 cut(s) 833
AspLEI GCGC 5 cut(s) 173, 557, 828, 849, 924
AsuC2I CCSGG 2 cut(s) 254, 281
AsuHPI GGTGA 3 cut(s) 344, 376, 760
AsuII TTCGAA 1 cut(s) 1167
AvrII CCTAGG 1 cut(s) 833
AxyI CCTNAGG 1 cut(s) 360
BamHI GGATCC 1 cut(s) 249
BanII GRGCYC 1 cut(s) 401
BauI CACGAG 3 cut(s) 233, 244, 1098
Bbv12I GWGCWC 2 cut(s) 205, 401
BbvI GCAGC 6 cut(s) 259, 463, 628, 671, 1094, 1184
BccI CCATC 2 cut(s) 329, 1152
BceAI ACGGC 5 cut(s) 64, 73, 334, 355, 834
BciT130I CCWGG 1 cut(s) 1177
BcnI CCSGG 2 cut(s) 254, 281
BcoDI GTCTC 4 cut(s) 112, 177, 436, 962
BfaI CTAG 3 cut(s) 702, 834, 908
BfmI CTRYAG 1 cut(s) 1160
BfoI RGCGCY 2 cut(s) 829, 850
BglI GCCNNNNNGGC 1 cut(s) 473
BglII AGATCT 1 cut(s) 1094
BlnI CCTAGG 1 cut(s) 833
BmcAI AGTACT 1 cut(s) 741
Bme1390I CCNGG 3 cut(s) 254, 281, 1177
BmiI GGNNCC 3 cut(s) 51, 129, 251
BmrFI CCNGG 3 cut(s) 254, 281, 1177
BmsI GCATC 7 cut(s) 345, 357, 628, 655, 667, 727, 939
BplI GAGNNNNNCTC 4 cut(s) 227, 259, 757, 789
BpmI CTGGAG 1 cut(s) 23
Bpu14I TTCGAA 1 cut(s) 1167
BpuMI CCSGG 2 cut(s) 254, 281
BsaHI GRCGYC 1 cut(s) 440
BsaI GGTCTC 1 cut(s) 436
BsaJI CCNNGG 2 cut(s) 833, 1176
BsaXI ACNNNNNCTCC 2 cut(s) 1201, 1231
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 161
Bse118I RCCGGY 1 cut(s) 454
Bse1I ACTGG 4 cut(s) 40, 129, 581, 984
Bse21I CCTNAGG 1 cut(s) 360
Bse3DI GCAATG 1 cut(s) 1092
BseBI CCWGG 1 cut(s) 1177
BseDI CCNNGG 2 cut(s) 833, 1176
BseGI GGATG 4 cut(s) 430, 954, 1060, 1198
BseLI CCNNNNNNNGG 2 cut(s) 46, 161
BseMI GCAATG 1 cut(s) 1092
BseMII CTCAG 2 cut(s) 59, 351
BseNI ACTGG 4 cut(s) 40, 129, 581, 984
BseRI GAGGAG 3 cut(s) 26, 202, 205
BseX3I CGGCCG 1 cut(s) 267
BseXI GCAGC 6 cut(s) 259, 463, 628, 671, 1094, 1184
BseYI CCCAGC 1 cut(s) 759
Bsh1236I CGCG 2 cut(s) 171, 266
Bsh1285I CGRYCG 1 cut(s) 270
BshFI GGCC 2 cut(s) 165, 269
BsiEI CGRYCG 1 cut(s) 270
BsiHKAI GWGCWC 2 cut(s) 205, 401
BsiSI CCGG 4 cut(s) 254, 280, 455, 584
BslI CCNNNNNNNGG 2 cut(s) 46, 161
BsmAI GTCTC 4 cut(s) 112, 177, 436, 962
BsmBI CGTCTC 1 cut(s) 112
BsnI GGCC 2 cut(s) 165, 269
Bso31I GGTCTC 1 cut(s) 436
Bsp119I TTCGAA 1 cut(s) 1167
Bsp1286I GDGCHC 2 cut(s) 205, 401
Bsp143I GATC 7 cut(s) 249, 387, 571, 1094, 1134, 1195, 1238
BspACI CCGC 9 cut(s) 99, 169, 266, 270, 284, 347, 416, 437, 878
BspANI GGCC 2 cut(s) 165, 269
BspCNI CTCAG 2 cut(s) 60, 352
BspFNI CGCG 2 cut(s) 171, 266
BspLI GGNNCC 3 cut(s) 51, 129, 251
BspMAI CTGCAG 1 cut(s) 1164
BspPI GGATC 6 cut(s) 244, 257, 395, 579, 1129, 1190
BspT104I TTCGAA 1 cut(s) 1167
BspTNI GGTCTC 1 cut(s) 436
BsrDI GCAATG 1 cut(s) 1092
BsrFI RCCGGY 1 cut(s) 454
BsrI ACTGG 4 cut(s) 40, 129, 581, 984
BssAI RCCGGY 1 cut(s) 454
BssECI CCNNGG 2 cut(s) 833, 1176
BssMI GATC 7 cut(s) 249, 387, 571, 1094, 1134, 1195, 1238
BssNAI GTATAC 1 cut(s) 1130
BssNI GRCGYC 1 cut(s) 440
BssSI CACGAG 3 cut(s) 233, 244, 1098
BssT1I CCWWGG 1 cut(s) 833
Bst1107I GTATAC 1 cut(s) 1130
Bst2BI CACGAG 3 cut(s) 233, 244, 1098
Bst2UI CCWGG 1 cut(s) 1177
Bst4CI ACNGT 2 cut(s) 973, 1003
Bst6I CTCTTC 2 cut(s) 22, 29
BstACI GRCGYC 1 cut(s) 440
BstAPI GCANNNNNTGC 1 cut(s) 1214
BstBI TTCGAA 1 cut(s) 1167
BstC8I GCNNGC 4 cut(s) 682, 830, 954, 1184
BstDEI CTNAG 2 cut(s) 68, 360
BstF5I GGATG 4 cut(s) 430, 954, 1060, 1198
BstFNI CGCG 2 cut(s) 171, 266
BstH2I RGCGCY 2 cut(s) 829, 850
BstHHI GCGC 5 cut(s) 173, 557, 828, 849, 924
BstKTI GATC 7 cut(s) 252, 390, 574, 1097, 1137, 1198, 1241
BstMAI GTCTC 4 cut(s) 112, 177, 436, 962
BstMBI GATC 7 cut(s) 249, 387, 571, 1094, 1134, 1195, 1238
BstMCI CGRYCG 1 cut(s) 270
BstMWI GCNNNNNNNGC 8 cut(s) 85, 272, 281, 443, 473, 563, 609, 1214
BstNI CCWGG 1 cut(s) 1177
BstNSI RCATGY 2 cut(s) 684, 956
BstSCI CCNGG 3 cut(s) 252, 279, 1175
BstSFI CTRYAG 1 cut(s) 1160
BstUI CGCG 2 cut(s) 171, 266
BstV1I GCAGC 6 cut(s) 259, 463, 628, 671, 1094, 1184
BstX2I RGATCY 2 cut(s) 249, 1094
BstYI RGATCY 2 cut(s) 249, 1094
BstZ17I GTATAC 1 cut(s) 1130
BstZI CGGCCG 1 cut(s) 267
Bsu36I CCTNAGG 1 cut(s) 360
BsuRI GGCC 2 cut(s) 165, 269
BtsCI GGATG 4 cut(s) 430, 954, 1060, 1198
BtsIMutI CAGTG 1 cut(s) 574
Cac8I GCNNGC 4 cut(s) 682, 830, 954, 1184
CciNI GCGGCCGC 1 cut(s) 267
CfoI GCGC 5 cut(s) 173, 557, 828, 849, 924
Cfr10I RCCGGY 1 cut(s) 454
CseI GACGC 1 cut(s) 429
Csp6I GTAC 3 cut(s) 82, 740, 756
CviAII CATG 6 cut(s) 604, 681, 783, 858, 953, 1017
CviQI GTAC 3 cut(s) 82, 740, 756
DdeI CTNAG 2 cut(s) 68, 360
DpnI GATC 7 cut(s) 251, 389, 573, 1096, 1136, 1197, 1240
DpnII GATC 7 cut(s) 249, 387, 571, 1094, 1134, 1195, 1238
EaeI YGGCCR 1 cut(s) 267
EagI CGGCCG 1 cut(s) 267
Eam1104I CTCTTC 2 cut(s) 22, 29
EarI CTCTTC 2 cut(s) 22, 29
EciI GGCGGA 2 cut(s) 362, 431
Ecl136II GAGCTC 1 cut(s) 399
EclXI CGGCCG 1 cut(s) 267
Eco130I CCWWGG 1 cut(s) 833
Eco24I GRGCYC 1 cut(s) 401
Eco31I GGTCTC 1 cut(s) 436
Eco52I CGGCCG 1 cut(s) 267
Eco53kI GAGCTC 1 cut(s) 399
Eco57I CTGAAG 1 cut(s) 398
Eco81I CCTNAGG 1 cut(s) 360
EcoICRI GAGCTC 1 cut(s) 399
EcoRI GAATTC 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 1175
EcoT14I CCWWGG 1 cut(s) 833
EcoT22I ATGCAT 2 cut(s) 682, 954
EcoT38I GRGCYC 1 cut(s) 401
ErhI CCWWGG 1 cut(s) 833
Esp3I CGTCTC 1 cut(s) 112
FaeI CATG 6 cut(s) 607, 684, 786, 861, 956, 1020
FatI CATG 6 cut(s) 603, 680, 782, 857, 952, 1016
FauNDI CATATG 1 cut(s) 1036
FblI GTMKAC 1 cut(s) 1129
FokI GGATG 4 cut(s) 437, 961, 1067, 1205
FriOI GRGCYC 1 cut(s) 401
FspBI CTAG 3 cut(s) 702, 834, 908
GlaI GCGC 5 cut(s) 172, 556, 827, 848, 923
GsaI CCCAGC 1 cut(s) 763
GsuI CTGGAG 1 cut(s) 23
HaeII RGCGCY 2 cut(s) 829, 850
HaeIII GGCC 2 cut(s) 165, 269
HapII CCGG 4 cut(s) 254, 280, 455, 584
HgaI GACGC 1 cut(s) 429
HhaI GCGC 5 cut(s) 173, 557, 828, 849, 924
Hin1I GRCGYC 1 cut(s) 440
Hin1II CATG 6 cut(s) 607, 684, 786, 861, 956, 1020
Hin6I GCGC 5 cut(s) 171, 555, 826, 847, 922
HinP1I GCGC 5 cut(s) 171, 555, 826, 847, 922
HincII GTYRAC 1 cut(s) 813
HindII GTYRAC 1 cut(s) 813
HindIII AAGCTT 2 cut(s) 303, 610
HinfI GANTC 1 cut(s) 62
HpaI GTTAAC 1 cut(s) 813
HpaII CCGG 4 cut(s) 254, 280, 455, 584
HphI GGTGA 3 cut(s) 344, 376, 760
Hpy166II GTNNAC 3 cut(s) 84, 813, 1130
Hpy188I TCNGA 5 cut(s) 49, 69, 182, 313, 520
Hpy188III TCNNGA 6 cut(s) 290, 298, 569, 662, 674, 1100
Hpy8I GTNNAC 3 cut(s) 84, 813, 1130
Hpy99I CGWCG 1 cut(s) 445
HpyAV CCTTC 3 cut(s) 41, 146, 373
HpyCH4III ACNGT 2 cut(s) 973, 1003
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 8 cut(s) 668, 680, 782, 857, 952, 1072, 1152, 1162
HpyF10VI GCNNNNNNNGC 8 cut(s) 85, 272, 281, 443, 473, 563, 609, 1214
HpyF3I CTNAG 2 cut(s) 68, 360
HpySE526I ACGT 1 cut(s) 105
Hsp92I GRCGYC 1 cut(s) 440
Hsp92II CATG 6 cut(s) 607, 684, 786, 861, 956, 1020
HspAI GCGC 5 cut(s) 171, 555, 826, 847, 922
KspAI GTTAAC 1 cut(s) 813
Kzo9I GATC 7 cut(s) 249, 387, 571, 1094, 1134, 1195, 1238
LmnI GCTCC 5 cut(s) 49, 823, 844, 1146, 1222
Lsp1109I GCAGC 6 cut(s) 259, 463, 628, 671, 1094, 1184
LweI GCATC 7 cut(s) 345, 357, 628, 655, 667, 727, 939
MaeI CTAG 3 cut(s) 702, 834, 908
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 6 cut(s) 90, 372, 499, 704, 748, 903
MalI GATC 7 cut(s) 251, 389, 573, 1096, 1136, 1197, 1240
MboI GATC 7 cut(s) 249, 387, 571, 1094, 1134, 1195, 1238
MboII GAAGA 4 cut(s) 9, 16, 1115, 1248
MfeI CAATTG 1 cut(s) 1212
MflI RGATCY 2 cut(s) 249, 1094
MhlI GDGCHC 2 cut(s) 205, 401
MluCI AATT 5 cut(s) 293, 669, 795, 1107, 1212
Mph1103I ATGCAT 2 cut(s) 682, 954
MseI TTAA 5 cut(s) 591, 812, 885, 942, 1226
MslI CAYNNNNRTG 2 cut(s) 957, 1191
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 4 cut(s) 254, 280, 455, 584
MspR9I CCNGG 3 cut(s) 254, 281, 1177
MunI CAATTG 1 cut(s) 1212
MvaI CCWGG 1 cut(s) 1177
MvnI CGCG 2 cut(s) 171, 266
MwoI GCNNNNNNNGC 8 cut(s) 85, 272, 281, 443, 473, 563, 609, 1214
NciI CCSGG 2 cut(s) 254, 281
NdeI CATATG 1 cut(s) 1036
NdeII GATC 7 cut(s) 249, 387, 571, 1094, 1134, 1195, 1238
NlaIII CATG 6 cut(s) 607, 684, 786, 861, 956, 1020
NlaIV GGNNCC 3 cut(s) 51, 129, 251
NmeAIII GCCGAG 1 cut(s) 413
NmuCI GTSAC 3 cut(s) 90, 704, 748
NotI GCGGCCGC 1 cut(s) 267
NsiI ATGCAT 2 cut(s) 682, 954
NspI RCATGY 2 cut(s) 684, 956
NspV TTCGAA 1 cut(s) 1167
PaeI GCATGC 2 cut(s) 684, 956
PfeI GAWTC 1 cut(s) 62
PfoI TCCNGGA 1 cut(s) 252
PsiI TTATAA 1 cut(s) 1007
Psp124BI GAGCTC 1 cut(s) 401
Psp6I CCWGG 1 cut(s) 1175
PspFI CCCAGC 1 cut(s) 759
PspGI CCWGG 1 cut(s) 1175
PspN4I GGNNCC 3 cut(s) 51, 129, 251
PstI CTGCAG 1 cut(s) 1164
PsuI RGATCY 2 cut(s) 249, 1094
RsaI GTAC 3 cut(s) 83, 741, 757
RsaNI GTAC 3 cut(s) 82, 740, 756
RseI CAYNNNNRTG 2 cut(s) 957, 1191
SacI GAGCTC 1 cut(s) 401
SaqAI TTAA 5 cut(s) 591, 812, 885, 942, 1226
Sau3AI GATC 7 cut(s) 249, 387, 571, 1094, 1134, 1195, 1238
ScaI AGTACT 1 cut(s) 741
ScrFI CCNGG 3 cut(s) 254, 281, 1177
SduI GDGCHC 2 cut(s) 205, 401
SfaNI GCATC 7 cut(s) 345, 357, 628, 655, 667, 727, 939
SfcI CTRYAG 1 cut(s) 1160
SfuI TTCGAA 1 cut(s) 1167
SmiMI CAYNNNNRTG 2 cut(s) 957, 1191
SphI GCATGC 2 cut(s) 684, 956
Sse9I AATT 5 cut(s) 293, 669, 795, 1107, 1212
SsiI CCGC 9 cut(s) 99, 169, 266, 270, 284, 347, 416, 437, 878
SspI AATATT 1 cut(s) 694
SspMI CTAG 3 cut(s) 702, 834, 908
SstI GAGCTC 1 cut(s) 401
StyD4I CCNGG 3 cut(s) 252, 279, 1175
StyI CCWWGG 1 cut(s) 833
TaaI ACNGT 2 cut(s) 973, 1003
TaiI ACGT 1 cut(s) 108
TaqI TCGA 6 cut(s) 5, 27, 142, 299, 401, 1167
TasI AATT 5 cut(s) 293, 669, 795, 1107, 1212
TatI WGTACW 1 cut(s) 739
TauI GCSGC 4 cut(s) 269, 272, 440, 880
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 5 cut(s) 591, 812, 885, 942, 1226
Tru9I TTAA 5 cut(s) 591, 812, 885, 942, 1226
TscAI CASTG 1 cut(s) 581
TseFI GTSAC 3 cut(s) 90, 704, 748
TseI GCWGC 6 cut(s) 272, 476, 641, 684, 1082, 1172
Tsp45I GTSAC 3 cut(s) 90, 704, 748
TspDTI ATGAA 2 cut(s) 1071, 1103
TspGWI ACGGA 1 cut(s) 100
TspRI CASTG 1 cut(s) 581
XapI RAATTY 2 cut(s) 293, 795
XceI RCATGY 2 cut(s) 684, 956
XmaJI CCTAGG 1 cut(s) 833
XmiI GTMKAC 1 cut(s) 1129
XspI CTAG 3 cut(s) 702, 834, 908
ZrmI AGTACT 1 cut(s) 741
Zsp2I ATGCAT 2 cut(s) 682, 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.