RLG00000027144

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003324
Physical Location & Seq
Reverse (-)
8982696 .. 8985757
3062 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027144

Sequence Viewer

Length: 1164 bp
ATGTCGAAATCGTCCTCGGCCCTCTTCGAGCTTCTCCTTCAGTTATCGGAGCCGATCGCCGAATCTCTGAGCAAAACGCCGTCACAAAGCAGCAACGTCCCCGTGAAAGCTTTACTGGAGCCTCTACTTCCTCGCAACCCTAATCCTAACATCGACCTCCGCGCCTCAATCCGAGACTTCACTCTCGCCTGTGCTCTACTCTCCTCCTCCCAATCCTCAACACACGAGCTTCTCTCGTGGATCCCCGAACACCTCGCGGCAGCTGCTGACCGGGCGGTTCTGGAATTCTCGAAAGCTTACTCCGATTACGGCGAGAAATCTGGTGATGGTTTGGAGGTGGAGTTGATGCCTGAGGTGATGCCGTTACTGAAGGAGAGGATCAAGGAGAGCTCGATTGATAAATCGGCGGACGGGGATGAGGTCTCGGCGGCGTCGGCGAGAGTGCCGGTTGGGTTTGCCATTGTGGCTGCTTATCAGTTTAGGTGGTTTGTTACACAGGGGCAAGGCATGATAAGCTTTATACATATCGCCAAAAGTGTAGATGCTGCTGAACTAGCTTGGTGTCAAGATGCAATTCTTGATGCATGCTGCCAGAATATTGCTTCTAGTGATGAGATATGGCATCTTGTGGTGGAGATGTCTGTACTTGTGGTGACTCGTACCCAGCAGAGTAATCCTCGTAGTGTATGGTTTGATAAAATTCTAAATGAGATGTTAAGTCACTTGGAGCGCCAGCCTAGGAACAAGGAGCGCCGTGTTGCATGGCTTCGTTATGTAGAGCCGCTTTTTAATGGTGTTGGTCTGGTGTTACTAGCTCATTTCAGGCGCATTTTCCCTCTTTTCTTTAAGTGGTTGCATGCTGATGATGATGAGACTGTTTTACTGGTTCTCAAACAGATTGAAACAGTTATAAAGCTAACATGGATTAGGAATACACCATATGTGGAAAGGTTGGTGGATGAACTTGCTACTTTGCACAAAGAAGCAGCATTGAAAAGATCTCGTGAAGAAATTAGAAATCTTGTTGTTCGTATATTGATCCTGCTCCATCAATGCAAGGGTCTGCAGTTCGAAGCAGCCTGGGGCAAGCACAGGGATGATCCAAACTTGGCAACAATTGCTCCCTCTTTAAGTGAAAGAAGATCAACAATGGCTTTCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

388

Amino Acids

43.66

Weight (kDa)

5.98

Isoelectric Point (pI)

52.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 911
AccII CGCG 2 cut(s) 162, 257
AciI CCGC 6 cut(s) 160, 257, 275, 407, 428, 782
AclWI GGATC 5 cut(s) 235, 248, 386, 1033, 1094
AcsI RAATTY 2 cut(s) 284, 699
AcuI CTGAAG 2 cut(s) 23, 389
AcyI GRCGYC 1 cut(s) 431
AfaI GTAC 2 cut(s) 645, 661
AgsI TTSAA 2 cut(s) 902, 994
AjnI CCWGG 1 cut(s) 1079
Alw21I GWGCWC 2 cut(s) 196, 392
Alw26I GTCTC 3 cut(s) 168, 427, 866
AlwI GGATC 5 cut(s) 235, 248, 386, 1033, 1094
AlwNI CAGNNNCTG 1 cut(s) 266
AoxI GGCC 1 cut(s) 18
ApeKI GCWGC 8 cut(s) 90, 260, 263, 467, 545, 588, 986, 1076
ApoI RAATTY 2 cut(s) 284, 699
AspA2I CCTAGG 1 cut(s) 737
AspLEI GCGC 4 cut(s) 164, 732, 753, 828
AspS9I GGNCC 1 cut(s) 19
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 3 cut(s) 335, 367, 664
AsuII TTCGAA 1 cut(s) 1071
AvrII CCTAGG 1 cut(s) 737
AxyI CCTNAGG 1 cut(s) 351
BamHI GGATCC 1 cut(s) 240
BanII GRGCYC 1 cut(s) 392
BauI CACGAG 3 cut(s) 224, 235, 1002
Bbv12I GWGCWC 2 cut(s) 196, 392
BbvI GCAGC 8 cut(s) 102, 250, 272, 454, 532, 575, 998, 1088
BccI CCATC 2 cut(s) 320, 1056
BceAI ACGGC 4 cut(s) 64, 325, 346, 738
BciT130I CCWGG 1 cut(s) 1081
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 3 cut(s) 168, 427, 866
BfaI CTAG 4 cut(s) 554, 606, 738, 812
BfmI CTRYAG 1 cut(s) 1064
BfoI RGCGCY 2 cut(s) 733, 754
BglI GCCNNNNNGGC 1 cut(s) 464
BglII AGATCT 1 cut(s) 998
BlnI CCTAGG 1 cut(s) 737
Bme1390I CCNGG 2 cut(s) 272, 1081
BmgT120I GGNCC 1 cut(s) 19
BmiI GGNNCC 3 cut(s) 51, 120, 242
BmrFI CCNGG 2 cut(s) 272, 1081
BmsI GCATC 6 cut(s) 336, 348, 532, 559, 571, 631
BplI GAGNNNNNCTC 4 cut(s) 218, 250, 661, 693
BpmI CTGGAG 1 cut(s) 137
Bpu14I TTCGAA 1 cut(s) 1071
BpuMI CCSGG 1 cut(s) 272
BsaHI GRCGYC 1 cut(s) 431
BsaI GGTCTC 1 cut(s) 427
BsaJI CCNNGG 3 cut(s) 15, 737, 1080
BsaXI ACNNNNNCTCC 4 cut(s) 326, 356, 1105, 1135
Bse118I RCCGGY 1 cut(s) 445
Bse1I ACTGG 2 cut(s) 120, 888
Bse21I CCTNAGG 1 cut(s) 351
BseBI CCWGG 1 cut(s) 1081
BseDI CCNNGG 3 cut(s) 15, 737, 1080
BseGI GGATG 3 cut(s) 421, 964, 1102
BseMII CTCAG 2 cut(s) 59, 342
BseNI ACTGG 2 cut(s) 120, 888
BseRI GAGGAG 2 cut(s) 193, 196
BseXI GCAGC 8 cut(s) 102, 250, 272, 454, 532, 575, 998, 1088
BseYI CCCAGC 1 cut(s) 663
Bsh1236I CGCG 2 cut(s) 162, 257
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 1 cut(s) 20
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 2 cut(s) 196, 392
BsiSI CCGG 2 cut(s) 271, 446
BslFI GGGAC 1 cut(s) 83
BsmAI GTCTC 3 cut(s) 168, 427, 866
BsmFI GGGAC 1 cut(s) 83
BsnI GGCC 1 cut(s) 20
Bso31I GGTCTC 1 cut(s) 427
Bsp119I TTCGAA 1 cut(s) 1071
Bsp1286I GDGCHC 2 cut(s) 196, 392
Bsp143I GATC 7 cut(s) 54, 240, 378, 998, 1038, 1099, 1142
BspACI CCGC 6 cut(s) 160, 257, 275, 407, 428, 782
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 2 cut(s) 60, 343
BspFNI CGCG 2 cut(s) 162, 257
BspLI GGNNCC 3 cut(s) 51, 120, 242
BspMAI CTGCAG 1 cut(s) 1068
BspPI GGATC 5 cut(s) 235, 248, 386, 1033, 1094
BspT104I TTCGAA 1 cut(s) 1071
BspTNI GGTCTC 1 cut(s) 427
BsrFI RCCGGY 1 cut(s) 445
BsrI ACTGG 2 cut(s) 120, 888
BssAI RCCGGY 1 cut(s) 445
BssECI CCNNGG 3 cut(s) 15, 737, 1080
BssMI GATC 7 cut(s) 54, 240, 378, 998, 1038, 1099, 1142
BssNI GRCGYC 1 cut(s) 431
BssSI CACGAG 3 cut(s) 224, 235, 1002
BssT1I CCWWGG 1 cut(s) 737
Bst2BI CACGAG 3 cut(s) 224, 235, 1002
Bst2UI CCWGG 1 cut(s) 1081
Bst4CI ACNGT 2 cut(s) 877, 907
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 1 cut(s) 431
BstAPI GCANNNNNTGC 1 cut(s) 1118
BstBI TTCGAA 1 cut(s) 1071
BstC8I GCNNGC 4 cut(s) 586, 734, 858, 1088
BstDEI CTNAG 2 cut(s) 68, 351
BstF5I GGATG 3 cut(s) 421, 964, 1102
BstFNI CGCG 2 cut(s) 162, 257
BstH2I RGCGCY 2 cut(s) 733, 754
BstHHI GCGC 4 cut(s) 164, 732, 753, 828
BstKTI GATC 7 cut(s) 57, 243, 381, 1001, 1041, 1102, 1145
BstMAI GTCTC 3 cut(s) 168, 427, 866
BstMBI GATC 7 cut(s) 54, 240, 378, 998, 1038, 1099, 1142
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 7 cut(s) 263, 272, 434, 464, 513, 554, 1118
BstNI CCWGG 1 cut(s) 1081
BstNSI RCATGY 2 cut(s) 588, 860
BstSCI CCNGG 2 cut(s) 270, 1079
BstSFI CTRYAG 1 cut(s) 1064
BstUI CGCG 2 cut(s) 162, 257
BstV1I GCAGC 8 cut(s) 102, 250, 272, 454, 532, 575, 998, 1088
BstX2I RGATCY 2 cut(s) 240, 998
BstYI RGATCY 2 cut(s) 240, 998
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 1 cut(s) 20
BtsCI GGATG 3 cut(s) 421, 964, 1102
Cac8I GCNNGC 4 cut(s) 586, 734, 858, 1088
CaiI CAGNNNCTG 1 cut(s) 266
CfoI GCGC 4 cut(s) 164, 732, 753, 828
Cfr10I RCCGGY 1 cut(s) 445
Cfr13I GGNCC 1 cut(s) 19
CseI GACGC 1 cut(s) 420
Csp6I GTAC 2 cut(s) 644, 660
CviAII CATG 5 cut(s) 508, 585, 762, 857, 921
CviQI GTAC 2 cut(s) 644, 660
DdeI CTNAG 2 cut(s) 68, 351
DpnI GATC 7 cut(s) 56, 242, 380, 1000, 1040, 1101, 1144
DpnII GATC 7 cut(s) 54, 240, 378, 998, 1038, 1099, 1142
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
EciI GGCGGA 1 cut(s) 422
Ecl136II GAGCTC 1 cut(s) 390
Eco130I CCWWGG 1 cut(s) 737
Eco24I GRGCYC 1 cut(s) 392
Eco31I GGTCTC 1 cut(s) 427
Eco53kI GAGCTC 1 cut(s) 390
Eco57I CTGAAG 2 cut(s) 23, 389
Eco81I CCTNAGG 1 cut(s) 351
EcoICRI GAGCTC 1 cut(s) 390
EcoRI GAATTC 1 cut(s) 284
EcoRII CCWGG 1 cut(s) 1079
EcoT14I CCWWGG 1 cut(s) 737
EcoT22I ATGCAT 1 cut(s) 586
EcoT38I GRGCYC 1 cut(s) 392
ErhI CCWWGG 1 cut(s) 737
FaeI CATG 5 cut(s) 511, 588, 765, 860, 924
FaqI GGGAC 1 cut(s) 83
FatI CATG 5 cut(s) 507, 584, 761, 856, 920
FauNDI CATATG 1 cut(s) 940
FokI GGATG 3 cut(s) 428, 971, 1109
FriOI GRGCYC 1 cut(s) 392
FspBI CTAG 4 cut(s) 554, 606, 738, 812
GlaI GCGC 4 cut(s) 163, 731, 752, 827
GsaI CCCAGC 1 cut(s) 667
GsuI CTGGAG 1 cut(s) 137
HaeII RGCGCY 2 cut(s) 733, 754
HaeIII GGCC 1 cut(s) 20
HapII CCGG 2 cut(s) 271, 446
HgaI GACGC 1 cut(s) 420
HhaI GCGC 4 cut(s) 164, 732, 753, 828
Hin1I GRCGYC 1 cut(s) 431
Hin1II CATG 5 cut(s) 511, 588, 765, 860, 924
Hin6I GCGC 4 cut(s) 162, 730, 751, 826
HinP1I GCGC 4 cut(s) 162, 730, 751, 826
HindIII AAGCTT 3 cut(s) 108, 294, 514
HinfI GANTC 2 cut(s) 62, 655
HpaII CCGG 2 cut(s) 271, 446
HphI GGTGA 3 cut(s) 335, 367, 664
Hpy188I TCNGA 4 cut(s) 49, 69, 173, 304
Hpy188III TCNNGA 5 cut(s) 281, 289, 566, 578, 1004
Hpy99I CGWCG 1 cut(s) 436
HpyAV CCTTC 2 cut(s) 47, 364
HpyCH4III ACNGT 2 cut(s) 877, 907
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 7 cut(s) 572, 584, 761, 856, 976, 1056, 1066
HpyF10VI GCNNNNNNNGC 7 cut(s) 263, 272, 434, 464, 513, 554, 1118
HpyF3I CTNAG 2 cut(s) 68, 351
HpySE526I ACGT 1 cut(s) 96
Hsp92I GRCGYC 1 cut(s) 431
Hsp92II CATG 5 cut(s) 511, 588, 765, 860, 924
HspAI GCGC 4 cut(s) 162, 730, 751, 826
Kzo9I GATC 7 cut(s) 54, 240, 378, 998, 1038, 1099, 1142
LmnI GCTCC 6 cut(s) 49, 118, 727, 748, 1050, 1126
Lsp1109I GCAGC 8 cut(s) 102, 250, 272, 454, 532, 575, 998, 1088
LweI GCATC 6 cut(s) 336, 348, 532, 559, 571, 631
MaeI CTAG 4 cut(s) 554, 606, 738, 812
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 6 cut(s) 81, 363, 490, 652, 719, 807
MalI GATC 7 cut(s) 56, 242, 380, 1000, 1040, 1101, 1144
MboI GATC 7 cut(s) 54, 240, 378, 998, 1038, 1099, 1142
MboII GAAGA 3 cut(s) 16, 1019, 1152
MfeI CAATTG 1 cut(s) 1116
MflI RGATCY 2 cut(s) 240, 998
MhlI GDGCHC 2 cut(s) 196, 392
MluCI AATT 5 cut(s) 284, 573, 699, 1011, 1116
MlyI GAGTC 1 cut(s) 649
Mph1103I ATGCAT 1 cut(s) 586
MseI TTAA 4 cut(s) 716, 789, 846, 1130
MslI CAYNNNNRTG 2 cut(s) 861, 1095
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 2 cut(s) 271, 446
MspR9I CCNGG 2 cut(s) 272, 1081
MunI CAATTG 1 cut(s) 1116
MvaI CCWGG 1 cut(s) 1081
MvnI CGCG 2 cut(s) 162, 257
MwoI GCNNNNNNNGC 7 cut(s) 263, 272, 434, 464, 513, 554, 1118
NciI CCSGG 1 cut(s) 272
NdeI CATATG 1 cut(s) 940
NdeII GATC 7 cut(s) 54, 240, 378, 998, 1038, 1099, 1142
NlaIII CATG 5 cut(s) 511, 588, 765, 860, 924
NlaIV GGNNCC 3 cut(s) 51, 120, 242
NmeAIII GCCGAG 1 cut(s) 404
NmuCI GTSAC 3 cut(s) 81, 652, 719
NsiI ATGCAT 1 cut(s) 586
NspI RCATGY 2 cut(s) 588, 860
NspV TTCGAA 1 cut(s) 1071
PaeI GCATGC 2 cut(s) 588, 860
PfeI GAWTC 1 cut(s) 62
Ple19I CGATCG 1 cut(s) 57
PleI GAGTC 1 cut(s) 649
PpsI GAGTC 1 cut(s) 649
PsiI TTATAA 1 cut(s) 911
Psp124BI GAGCTC 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 1079
PspFI CCCAGC 1 cut(s) 663
PspGI CCWGG 1 cut(s) 1079
PspN4I GGNNCC 3 cut(s) 51, 120, 242
PspPI GGNCC 1 cut(s) 19
PstI CTGCAG 1 cut(s) 1068
PstNI CAGNNNCTG 1 cut(s) 266
PsuI RGATCY 2 cut(s) 240, 998
PvuI CGATCG 1 cut(s) 57
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 2 cut(s) 645, 661
RsaNI GTAC 2 cut(s) 644, 660
RseI CAYNNNNRTG 2 cut(s) 861, 1095
SacI GAGCTC 1 cut(s) 392
SaqAI TTAA 4 cut(s) 716, 789, 846, 1130
Sau3AI GATC 7 cut(s) 54, 240, 378, 998, 1038, 1099, 1142
Sau96I GGNCC 1 cut(s) 19
SchI GAGTC 1 cut(s) 649
ScrFI CCNGG 2 cut(s) 272, 1081
SduI GDGCHC 2 cut(s) 196, 392
SfaNI GCATC 6 cut(s) 336, 348, 532, 559, 571, 631
SfcI CTRYAG 1 cut(s) 1064
SfuI TTCGAA 1 cut(s) 1071
SmiMI CAYNNNNRTG 2 cut(s) 861, 1095
SphI GCATGC 2 cut(s) 588, 860
Sse9I AATT 5 cut(s) 284, 573, 699, 1011, 1116
SsiI CCGC 6 cut(s) 160, 257, 275, 407, 428, 782
SspI AATATT 1 cut(s) 598
SspMI CTAG 4 cut(s) 554, 606, 738, 812
SstI GAGCTC 1 cut(s) 392
StyD4I CCNGG 2 cut(s) 270, 1079
StyI CCWWGG 1 cut(s) 737
TaaI ACNGT 2 cut(s) 877, 907
TaiI ACGT 1 cut(s) 99
TaqI TCGA 6 cut(s) 5, 27, 153, 290, 392, 1071
TasI AATT 5 cut(s) 284, 573, 699, 1011, 1116
TatI WGTACW 1 cut(s) 643
TauI GCSGC 3 cut(s) 260, 431, 784
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 4 cut(s) 716, 789, 846, 1130
Tru9I TTAA 4 cut(s) 716, 789, 846, 1130
TseFI GTSAC 3 cut(s) 81, 652, 719
TseI GCWGC 8 cut(s) 90, 260, 263, 467, 545, 588, 986, 1076
Tsp45I GTSAC 3 cut(s) 81, 652, 719
TspDTI ATGAA 1 cut(s) 975
XapI RAATTY 2 cut(s) 284, 699
XceI RCATGY 2 cut(s) 588, 860
XmaJI CCTAGG 1 cut(s) 737
XspI CTAG 4 cut(s) 554, 606, 738, 812
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.