Rorug01G0349200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003324
Physical Location & Seq
Reverse (-)
46682784 .. 46686392
3609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0349200.1

Sequence Viewer

Length: 1695 bp
ATGGAAGTTGAAGGGAAGCTCTCTGCCCACTCCACATCCAAAGAAGAAGAAGGAGAAGTTCATAGGGTTTGCGATGAGAATCAGGATTCTAAAGGAGGCTGGACCACCTTTCCCTTTGTTATTGCAACTGTGTTGGGTCTCTCGGTAGCAGTCGGCGGGTGGGGAGCAAATTTGATAGTGTTTCTGATAACAAAATTCAATGTGAAGACCATAACCGCTATACAGATCAATAACATCATCCTTGGTACGACCAATCTCCTCCCCATCGCTGGAGCCTTCATCGCTGATTCATTCTTCACCATATTTTCTGTCGTCAGTGCCTTTTCCTTCATTTCTTTACTGGGTGTGATCATGTTGACCCTAATAGCAATTATCCCTTCCTTGAGGCCCTCACCATGTTCAATTGACTCACTTATGACATGTCAAGCTCCATCAAAGCTTCAATACTCAGTCCTATATGCAGCATTATCACTAGTTTCACTAGGACTTGGAGGCACCCGCTTCACAATTGCAACCATGGGAGCAGACCAATTCAACAATCCGAATGATCAAGAGGTTTTCTTTAATTGGTATTTTGTGGCTTTGTATATGTCCAGTGTCTTTGGCGGCACTGTCATCGTATATATTCAGGACAACGTGGGATGGGGATTAGGGTTTGGAATTTGCTGCATTGCAAATGGTGTGGGATTGGGGGTGTTCTTATTGGGGAAACGATTTTATAAGCAAGTAAAACCAAAGGGGAGTCCATTCTTGAGCATAGCACGAGTATTGGTTGCAGCAATTCGGAAGAGGAAGATGTCATCAGAAACTTCAACAGAGCCTGATTACTATTATGGCGCAGGAGCAATGACTGACAATCCACCAACTAAAAGTATCAGATTCCTGAACTGTGCAGCCTTGAAAAGTGAAGAAGACAAACAATTTGTAGGCTCGTATGGAAACAGATGGAGGCTATGCACTGTGAAAGAAGTAGAAGACCTCAAAACCCTAATTAAGATCATGCCACTGTGGTCTACTGGCATCTTCCTAAGCACACCAATAGGCATTTTAAGCAGCCTCACTATACTCCAAGCCCTAACCATGGATAGACACCTTGGTCCACACTTCAAAATCCCTGCTGCTTCATTCCTAGTGTTCGCCCTCTTAGCCACAGCCATCTCCATCTTTATAATTGACCGCTTCATACTCCTCAAGTACAAGAATCTCACTGGACGAACTTTAACACCGATCCAGCGAATAGGGATCGGTCACATCATCAACATTATTTGTCTAATTGGGGCTGCCCTAATCGAAAGAAGACGACTTGGAGTGGTAAGATCACATAGCCTTATGAACCAACCTGGTTCAGAGGTGCCCATGTCCGCCTTTTGGCTTGTGGCACCGTTATCTGTTATGGGAATCGGTGAGGCATTTCATTTCCCTGGACAAGTTTCATTGTGCTATCAAGAATTTCCAAAATCATTGAGAAGTACATCCACTGCAATGATCTCATTATTACTTGGAATCGGGTATTATCTTAGCACTGCAGTCACTGATTTGGTAGACAAGACTACGAATTGGTTGCCAGATGATATAAATCAAGGGCGGTTGGATAATGTCTTTTGGATGTTGGTAGTGATTGGGGTGGTGAATTTTGGTTACTACATAACTTGTGCTAAGTTTTTTGTGTCCCAAAACCAAAATGTTGGGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

564

Amino Acids

61.75

Weight (kDa)

8.87

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 103 - 519 3.4e-68 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 720, 1169
AasI GACNNNNNNGTC 1 cut(s) 1095
AccB1I GGYRCC 3 cut(s) 494, 1351, 1378
AccI GTMKAC 2 cut(s) 1013, 1542
AciI CCGC 7 cut(s) 156, 216, 499, 606, 1177, 1362, 1585
AclWI GGATC 2 cut(s) 1222, 1250
AcsI RAATTY 5 cut(s) 169, 194, 660, 1448, 1630
AfaI GTAC 3 cut(s) 247, 1196, 1471
AfiI CCNNNNNNNGG 3 cut(s) 269, 1081, 1368
AflIII ACRYGT 1 cut(s) 419
AgsI TTSAA 8 cut(s) 11, 199, 402, 443, 535, 813, 901, 1108
AhlI ACTAGT 1 cut(s) 472
AjnI CCWGG 2 cut(s) 1339, 1420
AluBI AGCT 3 cut(s) 19, 428, 439
AluI AGCT 3 cut(s) 19, 428, 439
Alw26I GTCTC 1 cut(s) 143
AlwI GGATC 2 cut(s) 1222, 1250
AlwNI CAGNNNCTG 2 cut(s) 821, 1532
AoxI GGCC 1 cut(s) 386
ApeKI GCWGC 7 cut(s) 461, 666, 776, 893, 1053, 1118, 1280
ApoI RAATTY 5 cut(s) 169, 194, 660, 1448, 1630
ArsI GACNNNNNNTTYG 2 cut(s) 905, 937
AspLEI GCGC 1 cut(s) 839
AspS9I GGNCC 3 cut(s) 102, 387, 1097
AsuHPI GGTGA 4 cut(s) 289, 384, 1415, 1639
AvaII GGWCC 2 cut(s) 102, 1097
BaeGI GKGCMC 1 cut(s) 1356
BanI GGYRCC 3 cut(s) 494, 1351, 1378
BauI CACGAG 1 cut(s) 762
BbsI GAAGAC 4 cut(s) 212, 918, 981, 1303
BbvI GCAGC 7 cut(s) 473, 653, 788, 905, 1065, 1105, 1267
BccI CCATC 6 cut(s) 272, 439, 636, 939, 1163, 1169
BcgI CGANNNNNNTGC 4 cut(s) 598, 632, 1543, 1577
BciT130I CCWGG 2 cut(s) 1341, 1422
BclI TGATCA 2 cut(s) 348, 547
BcoDI GTCTC 1 cut(s) 143
BcuI ACTAGT 1 cut(s) 472
BfaI CTAG 3 cut(s) 473, 482, 1130
BfmI CTRYAG 1 cut(s) 1524
BisI GCNGC 8 cut(s) 462, 607, 667, 777, 894, 1054, 1119, 1281
BlsI GCNGC 8 cut(s) 463, 608, 668, 778, 895, 1055, 1120, 1282
Bme1390I CCNGG 2 cut(s) 1341, 1422
Bme18I GGWCC 2 cut(s) 102, 1097
BmgT120I GGNCC 3 cut(s) 102, 387, 1097
BmiI GGNNCC 4 cut(s) 274, 496, 1353, 1380
BmrFI CCNGG 2 cut(s) 1341, 1422
BmrI ACTGGG 1 cut(s) 350
BmsI GCATC 1 cut(s) 1029
BmuI ACTGGG 1 cut(s) 350
BpiI GAAGAC 4 cut(s) 212, 918, 981, 1303
BpmI CTGGAG 1 cut(s) 291
Bpu10I CCTNAGC 1 cut(s) 1028
BpuEI CTTGAG 3 cut(s) 403, 772, 1175
BsaBI GATNNNNATC 2 cut(s) 78, 1575
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 5 cut(s) 241, 516, 1080, 1093, 1420
Bsc4I CCNNNNNNNGG 3 cut(s) 269, 1081, 1368
Bse1I ACTGG 4 cut(s) 345, 594, 1021, 1213
Bse3DI GCAATG 3 cut(s) 669, 852, 1488
Bse8I GATNNNNATC 2 cut(s) 78, 1575
BseBI CCWGG 2 cut(s) 1341, 1422
BseDI CCNNGG 5 cut(s) 241, 516, 1080, 1093, 1420
BseGI GGATG 5 cut(s) 35, 237, 647, 1472, 1611
BseJI GATNNNNATC 2 cut(s) 78, 1575
BseLI CCNNNNNNNGG 3 cut(s) 269, 1081, 1368
BseMI GCAATG 3 cut(s) 669, 852, 1488
BseMII CTCAG 1 cut(s) 462
BseNI ACTGG 4 cut(s) 345, 594, 1021, 1213
BseRI GAGGAG 2 cut(s) 248, 1178
BseSI GKGCMC 1 cut(s) 1356
BseXI GCAGC 7 cut(s) 473, 653, 788, 905, 1065, 1105, 1267
BsgI GTGCAG 1 cut(s) 912
BshFI GGCC 1 cut(s) 388
BshNI GGYRCC 3 cut(s) 494, 1351, 1378
BslFI GGGAC 1 cut(s) 1654
BslI CCNNNNNNNGG 3 cut(s) 269, 1081, 1368
BsmAI GTCTC 1 cut(s) 143
BsmFI GGGAC 1 cut(s) 1654
BsnI GGCC 1 cut(s) 388
Bso31I GGTCTC 1 cut(s) 143
Bsp1286I GDGCHC 1 cut(s) 1356
Bsp143I GATC 8 cut(s) 225, 348, 547, 996, 1227, 1242, 1316, 1485
Bsp19I CCATGG 2 cut(s) 516, 1080
BspACI CCGC 7 cut(s) 156, 216, 499, 606, 1177, 1362, 1585
BspANI GGCC 1 cut(s) 388
BspCNI CTCAG 1 cut(s) 461
BspLI GGNNCC 4 cut(s) 274, 496, 1353, 1380
BspMAI CTGCAG 1 cut(s) 1528
BspPI GGATC 2 cut(s) 1222, 1250
BspT107I GGYRCC 3 cut(s) 494, 1351, 1378
BspTNI GGTCTC 1 cut(s) 143
BsrDI GCAATG 3 cut(s) 669, 852, 1488
BsrI ACTGG 4 cut(s) 345, 594, 1021, 1213
BssECI CCNNGG 5 cut(s) 241, 516, 1080, 1093, 1420
BssMI GATC 8 cut(s) 225, 348, 547, 996, 1227, 1242, 1316, 1485
BssSI CACGAG 1 cut(s) 762
BssT1I CCWWGG 4 cut(s) 241, 516, 1080, 1093
Bst2BI CACGAG 1 cut(s) 762
Bst2UI CCWGG 2 cut(s) 1341, 1422
Bst4CI ACNGT 6 cut(s) 130, 613, 890, 961, 1008, 1383
Bst6I CTCTTC 1 cut(s) 782
BstDEI CTNAG 5 cut(s) 448, 1028, 1144, 1517, 1656
BstDSI CCRYGG 2 cut(s) 516, 1080
BstF5I GGATG 5 cut(s) 35, 237, 647, 1472, 1611
BstHHI GCGC 1 cut(s) 839
BstKTI GATC 8 cut(s) 228, 351, 550, 999, 1230, 1245, 1319, 1488
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 8 cut(s) 225, 348, 547, 996, 1227, 1242, 1316, 1485
BstMWI GCNNNNNNNGC 3 cut(s) 281, 1050, 1145
BstNI CCWGG 2 cut(s) 1341, 1422
BstNSI RCATGY 1 cut(s) 423
BstSCI CCNGG 2 cut(s) 1339, 1420
BstSFI CTRYAG 1 cut(s) 1524
BstSLI GKGCMC 1 cut(s) 1356
BstV1I GCAGC 7 cut(s) 473, 653, 788, 905, 1065, 1105, 1267
BstV2I GAAGAC 4 cut(s) 212, 918, 981, 1303
BstXI CCANNNNNNTGG 1 cut(s) 1685
BsuRI GGCC 1 cut(s) 388
BtgI CCRYGG 2 cut(s) 516, 1080
BtgZI GCGATG 3 cut(s) 87, 250, 265
BtsCI GGATG 5 cut(s) 35, 237, 647, 1472, 1611
BtsI GCAGTG 2 cut(s) 1476, 1521
BtsIMutI CAGTG 9 cut(s) 322, 601, 609, 957, 1004, 1206, 1476, 1521, 1530
CaiI CAGNNNCTG 2 cut(s) 821, 1532
CfoI GCGC 1 cut(s) 839
Cfr13I GGNCC 3 cut(s) 102, 387, 1097
CsiI ACCWGGT 1 cut(s) 1339
Csp6I GTAC 3 cut(s) 246, 1195, 1470
CspCI CAANNNNNGTGG 2 cut(s) 663, 698
CviAII CATG 7 cut(s) 352, 396, 420, 517, 1000, 1081, 1357
CviQI GTAC 3 cut(s) 246, 1195, 1470
DdeI CTNAG 5 cut(s) 448, 1028, 1144, 1517, 1656
DpnI GATC 8 cut(s) 227, 350, 549, 998, 1229, 1244, 1318, 1487
DpnII GATC 8 cut(s) 225, 348, 547, 996, 1227, 1242, 1316, 1485
DrdI GACNNNNNNGTC 1 cut(s) 1095
DseDI GACNNNNNNGTC 1 cut(s) 1095
Eam1104I CTCTTC 1 cut(s) 782
EarI CTCTTC 1 cut(s) 782
EciI GGCGGA 1 cut(s) 1351
Eco130I CCWWGG 4 cut(s) 241, 516, 1080, 1093
Eco31I GGTCTC 1 cut(s) 143
Eco47I GGWCC 2 cut(s) 102, 1097
EcoO109I RGGNCCY 1 cut(s) 387
EcoRII CCWGG 2 cut(s) 1339, 1420
EcoT14I CCWWGG 4 cut(s) 241, 516, 1080, 1093
ErhI CCWWGG 4 cut(s) 241, 516, 1080, 1093
FaeI CATG 7 cut(s) 355, 399, 423, 520, 1003, 1084, 1360
FaqI GGGAC 1 cut(s) 1654
FatI CATG 7 cut(s) 351, 395, 419, 516, 999, 1080, 1356
FauI CCCGC 2 cut(s) 149, 506
FbaI TGATCA 2 cut(s) 348, 547
FblI GTMKAC 2 cut(s) 1013, 1542
Fnu4HI GCNGC 8 cut(s) 462, 607, 667, 777, 894, 1054, 1119, 1281
FokI GGATG 5 cut(s) 22, 224, 654, 1459, 1618
Fsp4HI GCNGC 8 cut(s) 462, 607, 667, 777, 894, 1054, 1119, 1281
FspBI CTAG 3 cut(s) 473, 482, 1130
GlaI GCGC 1 cut(s) 838
GluI GCNGC 8 cut(s) 462, 607, 667, 777, 894, 1054, 1119, 1281
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 1 cut(s) 388
HhaI GCGC 1 cut(s) 839
Hin1II CATG 7 cut(s) 355, 399, 423, 520, 1003, 1084, 1360
Hin6I GCGC 1 cut(s) 837
HinP1I GCGC 1 cut(s) 837
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 9 cut(s) 79, 86, 287, 407, 742, 879, 1201, 1398, 1503
HphI GGTGA 4 cut(s) 289, 384, 1415, 1639
Hpy166II GTNNAC 4 cut(s) 357, 1014, 1100, 1543
Hpy188I TCNGA 6 cut(s) 186, 543, 786, 805, 878, 1348
Hpy188III TCNNGA 6 cut(s) 83, 551, 629, 751, 883, 1445
Hpy8I GTNNAC 4 cut(s) 357, 1014, 1100, 1543
HpyAV CCTTC 5 cut(s) 5, 44, 286, 337, 387
HpyCH4III ACNGT 6 cut(s) 130, 613, 890, 961, 1008, 1383
HpyCH4IV ACGT 1 cut(s) 636
HpyF10VI GCNNNNNNNGC 3 cut(s) 281, 1050, 1145
HpyF3I CTNAG 5 cut(s) 448, 1028, 1144, 1517, 1656
HpySE526I ACGT 1 cut(s) 636
Hsp92II CATG 7 cut(s) 355, 399, 423, 520, 1003, 1084, 1360
HspAI GCGC 1 cut(s) 837
Ksp22I TGATCA 2 cut(s) 348, 547
Kzo9I GATC 8 cut(s) 225, 348, 547, 996, 1227, 1242, 1316, 1485
LmnI GCTCC 5 cut(s) 164, 272, 433, 521, 842
Lsp1109I GCAGC 7 cut(s) 473, 653, 788, 905, 1065, 1105, 1267
LweI GCATC 1 cut(s) 1029
MabI ACCWGGT 1 cut(s) 1339
MaeI CTAG 3 cut(s) 473, 482, 1130
MaeII ACGT 1 cut(s) 636
MaeIII GTNAC 3 cut(s) 1247, 1528, 1637
MalI GATC 8 cut(s) 227, 350, 549, 998, 1229, 1244, 1318, 1487
MboI GATC 8 cut(s) 225, 348, 547, 996, 1227, 1242, 1316, 1485
MfeI CAATTG 2 cut(s) 402, 507
MhlI GDGCHC 1 cut(s) 1356
MlyI GAGTC 2 cut(s) 401, 751
MmeI TCCRAC 1 cut(s) 1569
MseI TTAA 4 cut(s) 564, 993, 1049, 1220
MslI CAYNNNNRTG 1 cut(s) 1481
MspR9I CCNGG 2 cut(s) 1341, 1422
MunI CAATTG 2 cut(s) 402, 507
MvaI CCWGG 2 cut(s) 1341, 1422
MwoI GCNNNNNNNGC 3 cut(s) 281, 1050, 1145
NcoI CCATGG 2 cut(s) 516, 1080
NdeII GATC 8 cut(s) 225, 348, 547, 996, 1227, 1242, 1316, 1485
NlaIII CATG 7 cut(s) 355, 399, 423, 520, 1003, 1084, 1360
NlaIV GGNNCC 4 cut(s) 274, 496, 1353, 1380
NmuCI GTSAC 2 cut(s) 1247, 1528
NspI RCATGY 1 cut(s) 423
PciI ACATGT 1 cut(s) 419
PfeI GAWTC 7 cut(s) 79, 86, 287, 879, 1201, 1398, 1503
PkrI GCNGC 8 cut(s) 463, 608, 668, 778, 895, 1055, 1120, 1282
PleI GAGTC 2 cut(s) 401, 750
PpsI GAGTC 2 cut(s) 401, 750
PscI ACATGT 1 cut(s) 419
PsiI TTATAA 2 cut(s) 720, 1169
Psp6I CCWGG 2 cut(s) 1339, 1420
PspGI CCWGG 2 cut(s) 1339, 1420
PspN4I GGNNCC 4 cut(s) 274, 496, 1353, 1380
PspPI GGNCC 3 cut(s) 102, 387, 1097
PstI CTGCAG 1 cut(s) 1528
PstNI CAGNNNCTG 2 cut(s) 821, 1532
RsaI GTAC 3 cut(s) 247, 1196, 1471
RsaNI GTAC 3 cut(s) 246, 1195, 1470
RseI CAYNNNNRTG 1 cut(s) 1481
SaqAI TTAA 4 cut(s) 564, 993, 1049, 1220
SatI GCNGC 8 cut(s) 462, 607, 667, 777, 894, 1054, 1119, 1281
Sau3AI GATC 8 cut(s) 225, 348, 547, 996, 1227, 1242, 1316, 1485
Sau96I GGNCC 3 cut(s) 102, 387, 1097
SchI GAGTC 2 cut(s) 401, 751
ScrFI CCNGG 2 cut(s) 1341, 1422
SduI GDGCHC 1 cut(s) 1356
SexAI ACCWGGT 1 cut(s) 1339
SfaNI GCATC 1 cut(s) 1029
SfcI CTRYAG 1 cut(s) 1524
SinI GGWCC 2 cut(s) 102, 1097
SmiMI CAYNNNNRTG 1 cut(s) 1481
SmlI CTYRAG 3 cut(s) 382, 751, 1190
SmoI CTYRAG 3 cut(s) 382, 751, 1190
SpeI ACTAGT 1 cut(s) 472
SsiI CCGC 7 cut(s) 156, 216, 499, 606, 1177, 1362, 1585
SspMI CTAG 3 cut(s) 473, 482, 1130
StyD4I CCNGG 2 cut(s) 1339, 1420
StyI CCWWGG 4 cut(s) 241, 516, 1080, 1093
TaaI ACNGT 6 cut(s) 130, 613, 890, 961, 1008, 1383
TaiI ACGT 1 cut(s) 639
TaqI TCGA 1 cut(s) 1290
TaqII GACCGA 1 cut(s) 1235
TatI WGTACW 2 cut(s) 1194, 1469
TauI GCSGC 1 cut(s) 609
TfiI GAWTC 7 cut(s) 79, 86, 287, 879, 1201, 1398, 1503
Tru1I TTAA 4 cut(s) 564, 993, 1049, 1220
Tru9I TTAA 4 cut(s) 564, 993, 1049, 1220
TscAI CASTG 9 cut(s) 322, 601, 616, 964, 1011, 1213, 1483, 1528, 1537
TseFI GTSAC 2 cut(s) 1247, 1528
TseI GCWGC 7 cut(s) 461, 666, 776, 893, 1053, 1118, 1280
Tsp45I GTSAC 2 cut(s) 1247, 1528
TspDTI ATGAA 9 cut(s) 50, 268, 279, 319, 1113, 1171, 1346, 1403, 1422
TspRI CASTG 9 cut(s) 322, 601, 616, 964, 1011, 1213, 1483, 1528, 1537
VpaK11BI GGWCC 2 cut(s) 102, 1097
XapI RAATTY 5 cut(s) 169, 194, 660, 1448, 1630
XceI RCATGY 1 cut(s) 423
XmiI GTMKAC 2 cut(s) 1013, 1542
XspI CTAG 3 cut(s) 473, 482, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.