Rroxscaffold_6G00396450

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0003324
Physical Location & Seq
Reverse (-)
17962722 .. 17972014
9293 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00396450.1

Sequence Viewer

Length: 531 bp
ATGCCCGGTTTGATCGGCTCATCGGCGAGGCTCAACAACTCCGGCGGTTGTTCGACATCATTTGGTGCATTTATGGATCTTAATGGTTTTTTCAATTTAGTTTTGGGTTTTTCATTTGTGATTAGAAATGATGTTCTTCATTGCAATTCTGTGTGGAATCGAATGGTTGAGTTTGATAAAATTCTAAATGAGATGTTAGGTCACTTGGAGCGCCGGCTTAGGAACAAGAAGTGCTGTGTTGCATGGCTTCGTTACGTAGAGCCGCTTTTTAATGGTGTTGGTCTGGTGTTACTAGCTCATTTCAGGCGCATTTTCCCTCTTTTCTTTAAGTGGATGCAAGCTGATGACGATGAGATTATGCAAGGGTCTGCAGTTCGAAGCGACTGGGGCAAACACGGGGATGATCTAAACTTGGCAACAATTGCTCCCTCTTTAAGTCAAAGAAGATCAACAATGTGGCAGTGCGTGGCCCCACGCGCCACAGCAACCACCTCCGACGGCCACCAAAATTTGGCAGCACCATCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

19.74

Weight (kDa)

8.72

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 511
AccII CGCG 1 cut(s) 477
AciI CCGC 2 cut(s) 45, 263
AclWI GGATC 1 cut(s) 84
AcoI YGGCCR 1 cut(s) 499
AcsI RAATTY 2 cut(s) 180, 508
AfiI CCNNNNNNNGG 1 cut(s) 511
AgsI TTSAA 1 cut(s) 94
AluBI AGCT 2 cut(s) 296, 341
AluI AGCT 2 cut(s) 296, 341
AlwI GGATC 1 cut(s) 84
AoxI GGCC 2 cut(s) 468, 499
ApeKI GCWGC 1 cut(s) 515
ApoI RAATTY 2 cut(s) 180, 508
AspLEI GCGC 3 cut(s) 213, 309, 479
AspS9I GGNCC 1 cut(s) 469
AsuC2I CCSGG 1 cut(s) 6
AsuII TTCGAA 1 cut(s) 376
BbvI GCAGC 1 cut(s) 527
BccI CCATC 1 cut(s) 529
BceAI ACGGC 1 cut(s) 514
BcnI CCSGG 1 cut(s) 6
BfaI CTAG 1 cut(s) 293
BfmI CTRYAG 1 cut(s) 369
BfoI RGCGCY 1 cut(s) 214
BisI GCNGC 2 cut(s) 263, 516
BlsI GCNGC 2 cut(s) 264, 517
Bme1390I CCNGG 1 cut(s) 6
BmgT120I GGNCC 1 cut(s) 469
BmiI GGNNCC 1 cut(s) 471
BmrFI CCNGG 1 cut(s) 6
BmrI ACTGGG 1 cut(s) 394
BmsI GCATC 1 cut(s) 324
BmuI ACTGGG 1 cut(s) 394
Bpu10I CCTNAGC 1 cut(s) 218
Bpu14I TTCGAA 1 cut(s) 376
BpuMI CCSGG 1 cut(s) 6
BsaAI YACGTR 1 cut(s) 256
BsaXI ACNNNNNCTCC 2 cut(s) 409, 439
Bsc4I CCNNNNNNNGG 1 cut(s) 511
Bse118I RCCGGY 1 cut(s) 213
Bse1I ACTGG 1 cut(s) 389
Bse3DI GCAATG 1 cut(s) 139
BseGI GGATG 2 cut(s) 339, 406
BseLI CCNNNNNNNGG 1 cut(s) 511
BseMI GCAATG 1 cut(s) 139
BseNI ACTGG 1 cut(s) 389
BseXI GCAGC 1 cut(s) 527
Bsh1236I CGCG 1 cut(s) 477
BshFI GGCC 2 cut(s) 470, 501
BsiSI CCGG 3 cut(s) 6, 42, 214
BslI CCNNNNNNNGG 1 cut(s) 511
BsnI GGCC 2 cut(s) 470, 501
Bsp119I TTCGAA 1 cut(s) 376
Bsp143I GATC 4 cut(s) 12, 76, 403, 446
BspACI CCGC 2 cut(s) 45, 263
BspANI GGCC 2 cut(s) 470, 501
BspFNI CGCG 1 cut(s) 477
BspLI GGNNCC 1 cut(s) 471
BspMAI CTGCAG 1 cut(s) 373
BspPI GGATC 1 cut(s) 84
BspT104I TTCGAA 1 cut(s) 376
BsrDI GCAATG 1 cut(s) 139
BsrFI RCCGGY 1 cut(s) 213
BsrI ACTGG 1 cut(s) 389
BssAI RCCGGY 1 cut(s) 213
BssMI GATC 4 cut(s) 12, 76, 403, 446
BstAPI GCANNNNNTGC 1 cut(s) 422
BstBAI YACGTR 1 cut(s) 256
BstBI TTCGAA 1 cut(s) 376
BstC8I GCNNGC 2 cut(s) 215, 339
BstDEI CTNAG 1 cut(s) 218
BstF5I GGATG 2 cut(s) 339, 406
BstFNI CGCG 1 cut(s) 477
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 3 cut(s) 213, 309, 479
BstKTI GATC 4 cut(s) 15, 79, 406, 449
BstMBI GATC 4 cut(s) 12, 76, 403, 446
BstMWI GCNNNNNNNGC 3 cut(s) 387, 422, 476
BstSCI CCNGG 1 cut(s) 4
BstSFI CTRYAG 1 cut(s) 369
BstSNI TACGTA 1 cut(s) 256
BstUI CGCG 1 cut(s) 477
BstV1I GCAGC 1 cut(s) 527
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
BsuRI GGCC 2 cut(s) 470, 501
BtsCI GGATG 2 cut(s) 339, 406
BtsI GCAGTG 1 cut(s) 467
BtsIMutI CAGTG 1 cut(s) 467
Cac8I GCNNGC 2 cut(s) 215, 339
CfoI GCGC 3 cut(s) 213, 309, 479
Cfr10I RCCGGY 1 cut(s) 213
Cfr13I GGNCC 1 cut(s) 469
CviAII CATG 1 cut(s) 243
CviJI RGCY 9 cut(s) 18, 31, 217, 247, 262, 296, 341, 470, 501
CviKI_1 RGCY 9 cut(s) 18, 31, 217, 247, 262, 296, 341, 470, 501
DdeI CTNAG 1 cut(s) 218
DpnI GATC 4 cut(s) 14, 78, 405, 448
DpnII GATC 4 cut(s) 12, 76, 403, 446
EaeI YGGCCR 1 cut(s) 499
Eco105I TACGTA 1 cut(s) 256
FaeI CATG 1 cut(s) 246
FaiI YATR 4 cut(s) 74, 244, 359, 529
FatI CATG 1 cut(s) 242
Fnu4HI GCNGC 2 cut(s) 263, 516
FokI GGATG 2 cut(s) 346, 413
Fsp4HI GCNGC 2 cut(s) 263, 516
FspBI CTAG 1 cut(s) 293
GlaI GCGC 3 cut(s) 212, 308, 478
GluI GCNGC 2 cut(s) 263, 516
HaeII RGCGCY 1 cut(s) 214
HaeIII GGCC 2 cut(s) 470, 501
HapII CCGG 3 cut(s) 6, 42, 214
HhaI GCGC 3 cut(s) 213, 309, 479
Hin1II CATG 1 cut(s) 246
Hin6I GCGC 3 cut(s) 211, 307, 477
HinP1I GCGC 3 cut(s) 211, 307, 477
HinfI GANTC 1 cut(s) 157
HpaII CCGG 3 cut(s) 6, 42, 214
Hpy188I TCNGA 1 cut(s) 496
Hpy99I CGWCG 1 cut(s) 500
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 6 cut(s) 68, 144, 242, 337, 361, 371
HpyF10VI GCNNNNNNNGC 3 cut(s) 387, 422, 476
HpyF3I CTNAG 1 cut(s) 218
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 1 cut(s) 246
HspAI GCGC 3 cut(s) 211, 307, 477
KroI GCCGGC 1 cut(s) 213
KroNI GCCGGC 1 cut(s) 215
Kzo9I GATC 4 cut(s) 12, 76, 403, 446
LmnI GCTCC 2 cut(s) 208, 430
LpnPI CCDG 6 cut(s) 19, 55, 227, 269, 289, 370
Lsp1109I GCAGC 1 cut(s) 527
LweI GCATC 1 cut(s) 324
MaeI CTAG 1 cut(s) 293
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 3 cut(s) 200, 251, 288
MalI GATC 4 cut(s) 14, 78, 405, 448
MboI GATC 4 cut(s) 12, 76, 403, 446
MboII GAAGA 2 cut(s) 128, 456
MfeI CAATTG 1 cut(s) 420
MflI RGATCY 1 cut(s) 76
MluCI AATT 5 cut(s) 94, 145, 180, 420, 508
MmeI TCCRAC 1 cut(s) 519
MnlI CCTC 4 cut(s) 21, 327, 439, 502
MroNI GCCGGC 1 cut(s) 213
MseI TTAA 4 cut(s) 81, 270, 327, 434
MslI CAYNNNNRTG 1 cut(s) 399
MspI CCGG 3 cut(s) 6, 42, 214
MspR9I CCNGG 1 cut(s) 6
MunI CAATTG 1 cut(s) 420
MvnI CGCG 1 cut(s) 477
MwoI GCNNNNNNNGC 3 cut(s) 387, 422, 476
NaeI GCCGGC 1 cut(s) 215
NciI CCSGG 1 cut(s) 6
NdeII GATC 4 cut(s) 12, 76, 403, 446
NgoMIV GCCGGC 1 cut(s) 213
NlaIII CATG 1 cut(s) 246
NlaIV GGNNCC 1 cut(s) 471
NmuCI GTSAC 1 cut(s) 200
NspV TTCGAA 1 cut(s) 376
PdiI GCCGGC 1 cut(s) 215
PfeI GAWTC 1 cut(s) 157
PflMI CCANNNNNTGG 1 cut(s) 511
PkrI GCNGC 2 cut(s) 264, 517
Ppu21I YACGTR 1 cut(s) 256
PspN4I GGNNCC 1 cut(s) 471
PspPI GGNCC 1 cut(s) 469
PstI CTGCAG 1 cut(s) 373
PsuI RGATCY 1 cut(s) 76
RseI CAYNNNNRTG 1 cut(s) 399
SaqAI TTAA 4 cut(s) 81, 270, 327, 434
SatI GCNGC 2 cut(s) 263, 516
Sau3AI GATC 4 cut(s) 12, 76, 403, 446
Sau96I GGNCC 1 cut(s) 469
ScrFI CCNGG 1 cut(s) 6
SetI ASST 5 cut(s) 202, 258, 298, 343, 494
SfaNI GCATC 1 cut(s) 324
SfcI CTRYAG 1 cut(s) 369
SfuI TTCGAA 1 cut(s) 376
SmiMI CAYNNNNRTG 1 cut(s) 399
SnaBI TACGTA 1 cut(s) 256
Sse9I AATT 5 cut(s) 94, 145, 180, 420, 508
SsiI CCGC 2 cut(s) 45, 263
SspMI CTAG 1 cut(s) 293
StyD4I CCNGG 1 cut(s) 4
TaiI ACGT 1 cut(s) 258
TaqI TCGA 3 cut(s) 53, 160, 376
TasI AATT 5 cut(s) 94, 145, 180, 420, 508
TauI GCSGC 1 cut(s) 265
TfiI GAWTC 1 cut(s) 157
Tru1I TTAA 4 cut(s) 81, 270, 327, 434
Tru9I TTAA 4 cut(s) 81, 270, 327, 434
TscAI CASTG 1 cut(s) 467
TseFI GTSAC 1 cut(s) 200
TseI GCWGC 1 cut(s) 515
Tsp45I GTSAC 1 cut(s) 200
TspDTI ATGAA 2 cut(s) 102, 128
TspRI CASTG 1 cut(s) 467
Van91I CCANNNNNTGG 1 cut(s) 511
XapI RAATTY 2 cut(s) 180, 508
XspI CTAG 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.