RchiOBHm_Chr2g0086101

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002858
Physical Location & Seq
Reverse (-)
1267093 .. 1270974
3882 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46163

Sequence Viewer

Length: 3204 bp
ATGAGTGAGAAAGAAGCCATAGATTGTGAAGAAGGTTCATCAAGTTCTGATAGAACCAAAAAGGAAGAAGCTTTATTATGTTCTGCTAAGCTTCACATAGAAGAAATCCGGACCCAGAAATTCTCTATTGGTAAAAACCAATCTTTTCCTTTAACTCAAGACCTTCATTTTGCTGTAACCAACCTCTCAAAGCAGCTTTATCAAAAAGACTCAAACTTTTTGATGGAACTCATCCTAAATGCAGATGAGAATGAATACAGAGCAGATGTGGAGCCAACTTTGGAGTTTGTGATGACCCAAAAAGACATAACTGGGACTGGAGCACCAGCCACTCTGCTGGTTTTTAATAATGAGGTTGGATTTTCCAGGGAGAACATGGATTCCATTTGTGGTATGGGCCATTCTACAAAGAAGGACAAGAGACAGCAGAGTCTTATTGGAGAGAGAGGAAGTGGATTTAAAAGTGTATTTCTTGTGAGTTCACAGCCTCACATAATTAGCAATGGTTATTGTGTCAGATTCTTGGAAGAACCAGATCAAGATTGTGGTATTGGTTATATAGTTCCCCATTGGGTTAGTGGGGAGCCTTCTCTTTCTAGCATATTAGATGTATATGGGTTATGCAATGTCTTGCCTACTACCACGATTGTTCTTCCTCTGAAGCCTGAGAAGGTTGAAGCCGTGAGAGTTCAATTATCACAATTGCGTCCAGAGATTCTCTTGTTCTTGTCCAAGCTAAAGCAAGTACATGTACGCAGGTGTGATCCTGAAGCAACAGATGATTTCTCAAGGTTTTGTAAAACTAGTGGGACTGAACAAATGGAGTTATATAAAGCCACTTCACGTGTTGTTCAACTTTCAGTTAAGCAAAGAAAGGGTGATAGTGAGGAGTTGTGCAAGTATTACTTGTGGGAGGAGGCATTTCCTGTAAAACCTGCTAATAGAGTTGGTATAAGGATGAATGTGCAGAAATGGGTGATTACCCTTGCTTTTCCATCCAGAGAAAGGATGGGAAAGGCAACTTCTTCTGTTGGTATATTTGCTTGCCTACCCACGGAAATGGTCACAAACTTCCCATTTCTCATTCAAGCAGACTTCATTCTGAATGCTTCACGAGATTGTATACTGTTAGACAACGTATGGAACTTGGGAATTCTTGAGTGTATCCCATCATTATTTGTGGATGCTTTTCAGTCTCACTCCCTCCATTCTTCAGTGGATGAGCCATTTGAATATTTACCTGCTCAAGCTTCACCAATTCCTGAGTTTAACAAGGTAAAAGAGTCCATTAGAACTAGATTGCAAAGTTTAGTGATAGTGCCTTGCCAAATACTTGAGGGTACACTTTCCTTCGTGCTGCCTCAAGATGCAGTTCGAGTCCTGCCAAAATTTAGGGACCTAGTGATTGGAATTAGAGCAAAGGGTAATGTTTTGAGTGGTTCATCTTCATCTTTATTGAGGAAAGTCTTGCATCCGACTCTGGACCTTGAAAAGTACAATGCAGTTCTGAAGTTTCTAGATGTGAAATGTGCTGATGATCATTGGTATACAAAATGCGTCCAAGGTCTCTGCAACCCCACTGAGGATGAAATGGTTATAGCAGATAGCATTATTTCGTTGCTAGAGTGTATCAAGTTTCTGATGTCAAGCTCTCTTGATGATCCCTTGGTGCGTGACTTCATCAAGAGCATTGGAAAAAGCAGGTTTTTGAAGACCTTGAATGATTATAAAACACCAGAAGAATGTGTTCTGTTTGATCCTGCCTGGGAAAGTATTCTGAATCAGACAGATACACCGACCATTGATGTAAATTTTTACGGAACTGAAATTTTTCAGTACAAGAATCAGTTGAGAGATATTGGCGTGAAGGTAGATCCTTTGAGTGTATGTTCTCTTCTCTCTGGAATTCTGACCAAGCTGGCAAACCAGCCCCATTCTTGTTATACACCAGACACTACCTTTATAACGAGGATTTACAGCTTCCTCAACAAGTTCCACTGGCGTCCAGAAGCACCAGATGAAAGCCAATTTCAGGTGTGGATACCTGACCCTCCTCCAGGTGGATGGATTATGCCTCAGCAATGTGTACTTCATGTCAATCATGTTAGGAAGTATCTGTTTAGCTCATTCTTTTGGCTTGACGAACTTTACGCTGAAGAGCTCCTCCCCTTGTTTTCTTCAGCCTTTGGGGTTGCAGAAAATCCTTCCATCCATCATTACTTAAAGCTATGGGAAACATGGGCGTCATCGGAGAACTTGCAAGTGACTCTGGTAGAGTGCTGCTCCTTCTGGGAGTATGTTATAGACAACTGGAATCCAAAAGTTGAAGAGACTCTAAGACAGAAGTTGACAAAATTACCTGCCACAAAGTCAACAGCTGAAGAAATATATCTGATCAACAGAACAGAAGTGTTCCTAGCTGATGACTTGCAACTGAAGAAAACTTTCCTGAGCTATGACGAGGCCCCATTGTTCGTGTGGTTTCCCACAAGCAACTGCTTGTCATCTGTTCCTCCTAGAAGACTTTATGCCATTTACGAGTCCCTTGGAGTGAGGAAGGTGTCCAAGTCATTTGTGTGCAAACCGCATACCATCCTATTATTGGAGGAGCTGGATAAGATTGATGCACAAGATGGATTGATTGGCAGGGGTTTAATCAAGCTTATTTTGGGTTTTCTTGCTGGTCCGGAGGTTAATATGCCATTGAAAGAGAGACACAATGCTGCATGCTCACTCATTAAACTATCAGTGTATAAACTTGAGAGACCAAATCAAGTCATTTATCGACTGATGCCATCTGCTAGTACAACAGTGGATGTAAAAAAAGTGAAGCTAGTTACCTGGAAGAAGATTTCTCATCGTTTGCTCATTGATAACTCAGGCTACAGAAATGGAAAATCTGATCCAGAATTTGTTACAGCTTTCGCTGACGAACTCGCTCAAGGACTGTTAGTGCAGGCAAGACATTATGCAGCAGCACTGAGCAAACTTATCCAATGGGGATTTATGTTCGATTTTAATGAGAAGGAAGTGGATTTTTGGCTGATGAGAGAAAATTTAGAGTTGCCTATGGAAGATAAGGAGTTTCTTAGTGCTGCATTCCTCTCATCAACAGAACTTGTTAGAGGAAAGCGGGCATCTATGGAGACAGAGGAATCAGGCCCTTCCACAGCAATGCCTTCATGCAAGAAGCTGCGTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1067

Amino Acids

121.18

Weight (kDa)

5.77

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 71 - 172 3e-08 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1728, 1964
AarI CACCTGC 1 cut(s) 747
AasI GACNNNNNNGTC 1 cut(s) 429
Acc36I ACCTGC 5 cut(s) 747, 943, 1249, 1692, 2368
AccI GTMKAC 2 cut(s) 1123, 1547
AccIII TCCGGA 2 cut(s) 108, 2686
AciI CCGC 2 cut(s) 2585, 3133
AclWI GGATC 5 cut(s) 758, 1655, 1751, 1868, 2897
AcsI RAATTY 8 cut(s) 119, 1152, 1388, 1810, 1827, 1905, 2909, 3055
AcuI CTGAAG 8 cut(s) 680, 789, 1197, 1529, 2163, 2175, 2400, 2456
AcvI CACGTG 1 cut(s) 845
AcyI GRCGYC 2 cut(s) 2002, 2243
AfaI GTAC 7 cut(s) 747, 753, 1342, 1496, 1838, 2088, 2806
AfiI CCNNNNNNNGG 7 cut(s) 1005, 1054, 1582, 2032, 2057, 2060, 2602
AflIII ACRYGT 2 cut(s) 748, 844
AhlI ACTAGT 1 cut(s) 803
AjnI CCWGG 4 cut(s) 365, 1763, 2056, 2840
AloI GAACNNNNNNTCC 2 cut(s) 365, 397
Alw21I GWGCWC 2 cut(s) 325, 2163
Alw26I GTCTC 7 cut(s) 415, 1200, 1571, 2324, 2707, 2758, 3140
AlwI GGATC 5 cut(s) 758, 1655, 1751, 1868, 2897
Aor13HI TCCGGA 2 cut(s) 108, 2686
AoxI GGCC 3 cut(s) 397, 2463, 3160
ApeKI GCWGC 8 cut(s) 193, 1357, 2280, 2723, 2972, 2975, 3095, 3193
ApoI RAATTY 8 cut(s) 119, 1152, 1388, 1810, 1827, 1905, 2909, 3055
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
Asp700I GAANNNNTTC 4 cut(s) 1746, 1773, 1830, 2444
AspS9I GGNCC 7 cut(s) 111, 397, 1396, 1483, 2464, 2684, 3161
AsuHPI GGTGA 3 cut(s) 890, 988, 1245
AvaII GGWCC 4 cut(s) 111, 1396, 1483, 2684
BanII GRGCYC 1 cut(s) 2163
BarI GAAGNNNNNNTAC 2 cut(s) 1878, 1910
BauI CACGAG 1 cut(s) 1113
BbrPI CACGTG 1 cut(s) 845
BbsI GAAGAC 2 cut(s) 1718, 2527
Bbv12I GWGCWC 2 cut(s) 325, 2163
BbvCI CCTCAGC 1 cut(s) 2076
BbvI GCAGC 8 cut(s) 205, 1344, 2267, 2710, 2984, 2987, 3082, 3180
BceAI ACGGC 1 cut(s) 665
BciT130I CCWGG 4 cut(s) 367, 1765, 2058, 2842
BciVI GTATCC 2 cut(s) 1175, 2034
BclI TGATCA 2 cut(s) 1537, 2394
BcoDI GTCTC 7 cut(s) 415, 1200, 1571, 2324, 2707, 2758, 3140
BcuI ACTAGT 1 cut(s) 803
BfmI CTRYAG 1 cut(s) 2884
BfuAI ACCTGC 5 cut(s) 747, 943, 1249, 1692, 2368
BfuI GTATCC 2 cut(s) 1175, 2034
BisI GCNGC 8 cut(s) 194, 1358, 2281, 2724, 2973, 2976, 3096, 3194
BlpI GCTNAGC 1 cut(s) 87
BlsI GCNGC 8 cut(s) 195, 1359, 2282, 2725, 2974, 2977, 3097, 3195
Bme1390I CCNGG 4 cut(s) 367, 1765, 2058, 2842
Bme18I GGWCC 4 cut(s) 111, 1396, 1483, 2684
BmgT120I GGNCC 7 cut(s) 111, 397, 1396, 1483, 2464, 2684, 3161
BmiI GGNNCC 5 cut(s) 113, 273, 585, 1397, 2466
BmrFI CCNGG 4 cut(s) 367, 1765, 2058, 2842
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 6 cut(s) 1174, 1357, 1480, 2614, 2781, 3146
BmuI ACTGGG 1 cut(s) 321
BpiI GAAGAC 2 cut(s) 1718, 2527
BplI GAGNNNNNCTC 2 cut(s) 2267, 2299
BpmI CTGGAG 2 cut(s) 339, 2040
Bpu10I CCTNAGC 2 cut(s) 2076, 2450
Bpu1102I GCTNAGC 1 cut(s) 87
BpuEI CTTGAG 8 cut(s) 141, 772, 1178, 1230, 1347, 1355, 2780, 2925
BsaAI YACGTR 1 cut(s) 845
BsaHI GRCGYC 2 cut(s) 2002, 2243
BsaI GGTCTC 2 cut(s) 1571, 2758
BsaJI CCNNGG 6 cut(s) 366, 1053, 1561, 1665, 1764, 2545
BsaWI WCCGGW 2 cut(s) 108, 2686
Bsc4I CCNNNNNNNGG 7 cut(s) 1005, 1054, 1582, 2032, 2057, 2060, 2602
Bse1I ACTGG 4 cut(s) 316, 322, 2003, 2315
Bse3DI GCAATG 4 cut(s) 508, 631, 2087, 3180
BseAI TCCGGA 2 cut(s) 108, 2686
BseBI CCWGG 4 cut(s) 367, 1765, 2058, 2842
BseDI CCNNGG 6 cut(s) 366, 1053, 1561, 1665, 1764, 2545
BseLI CCNNNNNNNGG 7 cut(s) 1005, 1054, 1582, 2032, 2057, 2060, 2602
BseMI GCAATG 4 cut(s) 508, 631, 2087, 3180
BseMII CTCAG 7 cut(s) 657, 1254, 1572, 2090, 2441, 2892, 2972
BseNI ACTGG 4 cut(s) 316, 322, 2003, 2315
BseRI GAGGAG 5 cut(s) 902, 929, 2043, 2153, 2621
BseXI GCAGC 8 cut(s) 205, 1344, 2267, 2710, 2984, 2987, 3082, 3180
BsgI GTGCAG 2 cut(s) 986, 2975
BshFI GGCC 3 cut(s) 399, 2465, 3162
BsiHKAI GWGCWC 2 cut(s) 325, 2163
BsiSI CCGG 2 cut(s) 109, 2687
BslFI GGGAC 4 cut(s) 328, 823, 1409, 2527
BslI CCNNNNNNNGG 7 cut(s) 1005, 1054, 1582, 2032, 2057, 2060, 2602
BsmAI GTCTC 7 cut(s) 415, 1200, 1571, 2324, 2707, 2758, 3140
BsmFI GGGAC 4 cut(s) 328, 823, 1409, 2527
BsmI GAATGC 2 cut(s) 1111, 3098
BsnI GGCC 3 cut(s) 399, 2465, 3162
Bso31I GGTCTC 2 cut(s) 1571, 2758
Bsp1286I GDGCHC 2 cut(s) 325, 2163
Bsp13I TCCGGA 2 cut(s) 108, 2686
Bsp143I GATC 8 cut(s) 535, 763, 1537, 1660, 1756, 1873, 2394, 2902
Bsp1720I GCTNAGC 1 cut(s) 87
BspACI CCGC 2 cut(s) 2585, 3133
BspANI GGCC 3 cut(s) 399, 2465, 3162
BspCNI CTCAG 7 cut(s) 658, 1255, 1573, 2089, 2442, 2891, 2973
BspEI TCCGGA 2 cut(s) 108, 2686
BspLI GGNNCC 5 cut(s) 113, 273, 585, 1397, 2466
BspMI ACCTGC 5 cut(s) 747, 943, 1249, 1692, 2368
BspPI GGATC 5 cut(s) 758, 1655, 1751, 1868, 2897
BspQI GCTCTTC 1 cut(s) 2151
BspTNI GGTCTC 2 cut(s) 1571, 2758
BsrDI GCAATG 4 cut(s) 508, 631, 2087, 3180
BsrI ACTGG 4 cut(s) 316, 322, 2003, 2315
BssECI CCNNGG 6 cut(s) 366, 1053, 1561, 1665, 1764, 2545
BssMI GATC 8 cut(s) 535, 763, 1537, 1660, 1756, 1873, 2394, 2902
BssNAI GTATAC 2 cut(s) 1124, 1548
BssNI GRCGYC 2 cut(s) 2002, 2243
BssSI CACGAG 1 cut(s) 1113
BssT1I CCWWGG 3 cut(s) 1561, 1665, 2545
Bst1107I GTATAC 2 cut(s) 1124, 1548
Bst2BI CACGAG 1 cut(s) 1113
Bst2UI CCWGG 4 cut(s) 367, 1765, 2058, 2842
Bst4CI ACNGT 3 cut(s) 1128, 2812, 2949
Bst6I CTCTTC 3 cut(s) 1899, 2151, 2322
BstACI GRCGYC 2 cut(s) 2002, 2243
BstBAI YACGTR 1 cut(s) 845
BstC8I GCNNGC 5 cut(s) 1045, 1920, 2728, 2958, 3135
BstDSI CCRYGG 1 cut(s) 1053
BstENI CCTNNNNNAGG 1 cut(s) 2055
BstKTI GATC 8 cut(s) 538, 766, 1540, 1663, 1759, 1876, 2397, 2905
BstMAI GTCTC 7 cut(s) 415, 1200, 1571, 2324, 2707, 2758, 3140
BstMBI GATC 8 cut(s) 535, 763, 1537, 1660, 1756, 1873, 2394, 2902
BstNI CCWGG 4 cut(s) 367, 1765, 2058, 2842
BstNSI RCATGY 2 cut(s) 752, 2730
BstSCI CCNGG 4 cut(s) 365, 1763, 2056, 2840
BstSFI CTRYAG 1 cut(s) 2884
BstV1I GCAGC 8 cut(s) 205, 1344, 2267, 2710, 2984, 2987, 3082, 3180
BstV2I GAAGAC 2 cut(s) 1718, 2527
BstX2I RGATCY 1 cut(s) 1873
BstXI CCANNNNNNTGG 3 cut(s) 337, 1060, 2064
BstYI RGATCY 1 cut(s) 1873
BstZ17I GTATAC 2 cut(s) 1124, 1548
BsuI GTATCC 2 cut(s) 1175, 2034
BsuRI GGCC 3 cut(s) 399, 2465, 3162
BtgI CCRYGG 1 cut(s) 1053
BtsIMutI CAGTG 6 cut(s) 1221, 1578, 1996, 2754, 2817, 2978
BveI ACCTGC 5 cut(s) 747, 943, 1249, 1692, 2368
Cac8I GCNNGC 5 cut(s) 1045, 1920, 2728, 2958, 3135
Cfr13I GGNCC 7 cut(s) 111, 397, 1396, 1483, 2464, 2684, 3161
CseI GACGC 5 cut(s) 695, 1546, 1991, 2232, 3185
Csp6I GTAC 7 cut(s) 746, 752, 1341, 1495, 1837, 2087, 2805
CviAII CATG 7 cut(s) 376, 749, 2093, 2102, 2238, 2727, 3183
CviQI GTAC 7 cut(s) 746, 752, 1341, 1495, 1837, 2087, 2805
DpnI GATC 8 cut(s) 537, 765, 1539, 1662, 1758, 1875, 2396, 2904
DpnII GATC 8 cut(s) 535, 763, 1537, 1660, 1756, 1873, 2394, 2902
DraI TTTAAA 1 cut(s) 460
DrdI GACNNNNNNGTC 1 cut(s) 429
DseDI GACNNNNNNGTC 1 cut(s) 429
Eam1104I CTCTTC 3 cut(s) 1899, 2151, 2322
EarI CTCTTC 3 cut(s) 1899, 2151, 2322
Ecl136II GAGCTC 1 cut(s) 2161
Eco130I CCWWGG 3 cut(s) 1561, 1665, 2545
Eco24I GRGCYC 1 cut(s) 2163
Eco31I GGTCTC 2 cut(s) 1571, 2758
Eco47I GGWCC 4 cut(s) 111, 1396, 1483, 2684
Eco53kI GAGCTC 1 cut(s) 2161
Eco57I CTGAAG 8 cut(s) 680, 789, 1197, 1529, 2163, 2175, 2400, 2456
Eco72I CACGTG 1 cut(s) 845
EcoICRI GAGCTC 1 cut(s) 2161
EcoNI CCTNNNNNAGG 1 cut(s) 2055
EcoO109I RGGNCCY 3 cut(s) 1396, 2464, 3161
EcoRI GAATTC 2 cut(s) 1152, 1905
EcoRII CCWGG 4 cut(s) 365, 1763, 2056, 2840
EcoT14I CCWWGG 3 cut(s) 1561, 1665, 2545
EcoT38I GRGCYC 1 cut(s) 2163
ErhI CCWWGG 3 cut(s) 1561, 1665, 2545
FaeI CATG 7 cut(s) 379, 752, 2096, 2105, 2241, 2730, 3186
FalI AAGNNNNNCTT 2 cut(s) 890, 922
FaqI GGGAC 4 cut(s) 328, 823, 1409, 2527
FatI CATG 7 cut(s) 375, 748, 2092, 2101, 2237, 2726, 3182
FauI CCCGC 1 cut(s) 3126
FbaI TGATCA 2 cut(s) 1537, 2394
FblI GTMKAC 2 cut(s) 1123, 1547
Fnu4HI GCNGC 8 cut(s) 194, 1358, 2281, 2724, 2973, 2976, 3096, 3194
FriOI GRGCYC 1 cut(s) 2163
Fsp4HI GCNGC 8 cut(s) 194, 1358, 2281, 2724, 2973, 2976, 3096, 3194
GluI GCNGC 8 cut(s) 194, 1358, 2281, 2724, 2973, 2976, 3096, 3194
GsuI CTGGAG 2 cut(s) 339, 2040
HaeIII GGCC 3 cut(s) 399, 2465, 3162
HapII CCGG 2 cut(s) 109, 2687
HgaI GACGC 5 cut(s) 695, 1546, 1991, 2232, 3185
Hin1I GRCGYC 2 cut(s) 2002, 2243
Hin1II CATG 7 cut(s) 379, 752, 2096, 2105, 2241, 2730, 3186
HincII GTYRAC 2 cut(s) 2349, 2373
HindII GTYRAC 2 cut(s) 2349, 2373
HindIII AAGCTT 4 cut(s) 69, 89, 1248, 2660
HpaII CCGG 2 cut(s) 109, 2687
HphI GGTGA 3 cut(s) 890, 988, 1245
Hpy166II GTNNAC 7 cut(s) 482, 1124, 1343, 1548, 2087, 2349, 2373
Hpy8I GTNNAC 7 cut(s) 482, 1124, 1343, 1548, 2087, 2349, 2373
HpyCH4III ACNGT 3 cut(s) 1128, 2812, 2949
HpyCH4IV ACGT 2 cut(s) 844, 1137
HpySE526I ACGT 2 cut(s) 844, 1137
Hsp92I GRCGYC 2 cut(s) 2002, 2243
Hsp92II CATG 7 cut(s) 379, 752, 2096, 2105, 2241, 2730, 3186
Kpn2I TCCGGA 2 cut(s) 108, 2686
Ksp22I TGATCA 2 cut(s) 1537, 2394
Kzo9I GATC 8 cut(s) 535, 763, 1537, 1660, 1756, 1873, 2394, 2902
LguI GCTCTTC 1 cut(s) 2151
LmnI GCTCC 6 cut(s) 271, 320, 583, 2166, 2288, 2608
Lsp1109I GCAGC 8 cut(s) 205, 1344, 2267, 2710, 2984, 2987, 3082, 3180
LweI GCATC 6 cut(s) 1174, 1357, 1480, 2614, 2781, 3146
MaeII ACGT 2 cut(s) 844, 1137
MaeIII GTNAC 6 cut(s) 175, 1063, 1673, 2263, 2836, 2914
MalI GATC 8 cut(s) 537, 765, 1539, 1662, 1758, 1875, 2396, 2904
MboI GATC 8 cut(s) 535, 763, 1537, 1660, 1756, 1873, 2394, 2902
MfeI CAATTG 1 cut(s) 701
MflI RGATCY 1 cut(s) 1873
MhlI GDGCHC 2 cut(s) 325, 2163
MlyI GAGTC 8 cut(s) 203, 439, 1292, 1386, 1471, 2260, 2326, 2549
MmeI TCCRAC 2 cut(s) 337, 1499
MroI TCCGGA 2 cut(s) 108, 2686
MroXI GAANNNNTTC 4 cut(s) 1746, 1773, 1830, 2444
MslI CAYNNNNRTG 3 cut(s) 1058, 2575, 3173
MspA1I CMGCKG 1 cut(s) 2378
MspI CCGG 2 cut(s) 109, 2687
MspR9I CCNGG 4 cut(s) 367, 1765, 2058, 2842
MunI CAATTG 1 cut(s) 701
Mva1269I GAATGC 2 cut(s) 1111, 3098
MvaI CCWGG 4 cut(s) 367, 1765, 2058, 2842
NdeII GATC 8 cut(s) 535, 763, 1537, 1660, 1756, 1873, 2394, 2902
NlaIII CATG 7 cut(s) 379, 752, 2096, 2105, 2241, 2730, 3186
NlaIV GGNNCC 5 cut(s) 113, 273, 585, 1397, 2466
NmuCI GTSAC 3 cut(s) 1063, 1673, 2263
NspI RCATGY 2 cut(s) 752, 2730
PaeI GCATGC 1 cut(s) 2730
PaqCI CACCTGC 1 cut(s) 747
PciI ACATGT 1 cut(s) 748
PciSI GCTCTTC 1 cut(s) 2151
PctI GAATGC 2 cut(s) 1111, 3098
PdmI GAANNNNTTC 4 cut(s) 1746, 1773, 1830, 2444
PfeI GAWTC 7 cut(s) 380, 519, 715, 1780, 1843, 2314, 3155
PkrI GCNGC 8 cut(s) 195, 1359, 2282, 2725, 2974, 2977, 3097, 3195
PleI GAGTC 8 cut(s) 203, 438, 1291, 1385, 1471, 2260, 2326, 2548
PmaCI CACGTG 1 cut(s) 845
PmlI CACGTG 1 cut(s) 845
PpsI GAGTC 8 cut(s) 203, 438, 1291, 1385, 1471, 2260, 2326, 2548
Ppu21I YACGTR 1 cut(s) 845
PpuMI RGGWCCY 1 cut(s) 1396
PscI ACATGT 1 cut(s) 748
PsiI TTATAA 2 cut(s) 1728, 1964
Psp124BI GAGCTC 1 cut(s) 2163
Psp5II RGGWCCY 1 cut(s) 1396
Psp6I CCWGG 4 cut(s) 365, 1763, 2056, 2840
PspCI CACGTG 1 cut(s) 845
PspGI CCWGG 4 cut(s) 365, 1763, 2056, 2840
PspN4I GGNNCC 5 cut(s) 113, 273, 585, 1397, 2466
PspPI GGNCC 7 cut(s) 111, 397, 1396, 1483, 2464, 2684, 3161
PspPPI RGGWCCY 1 cut(s) 1396
PsuI RGATCY 1 cut(s) 1873
PvuII CAGCTG 1 cut(s) 2378
RsaI GTAC 7 cut(s) 747, 753, 1342, 1496, 1838, 2088, 2806
RsaNI GTAC 7 cut(s) 746, 752, 1341, 1495, 1837, 2087, 2805
RseI CAYNNNNRTG 3 cut(s) 1058, 2575, 3173
SacI GAGCTC 1 cut(s) 2163
SapI GCTCTTC 1 cut(s) 2151
SatI GCNGC 8 cut(s) 194, 1358, 2281, 2724, 2973, 2976, 3096, 3194
Sau3AI GATC 8 cut(s) 535, 763, 1537, 1660, 1756, 1873, 2394, 2902
Sau96I GGNCC 7 cut(s) 111, 397, 1396, 1483, 2464, 2684, 3161
SchI GAGTC 8 cut(s) 203, 439, 1292, 1386, 1471, 2260, 2326, 2549
ScrFI CCNGG 4 cut(s) 367, 1765, 2058, 2842
SduI GDGCHC 2 cut(s) 325, 2163
SfaNI GCATC 6 cut(s) 1174, 1357, 1480, 2614, 2781, 3146
SfcI CTRYAG 1 cut(s) 2884
SinI GGWCC 4 cut(s) 111, 1396, 1483, 2684
SmiMI CAYNNNNRTG 3 cut(s) 1058, 2575, 3173
SmlI CTYRAG 8 cut(s) 156, 787, 1157, 1245, 1334, 1362, 2759, 2940
SmoI CTYRAG 8 cut(s) 156, 787, 1157, 1245, 1334, 1362, 2759, 2940
SpeI ACTAGT 1 cut(s) 803
SphI GCATGC 1 cut(s) 2730
SsiI CCGC 2 cut(s) 2585, 3133
SspI AATATT 1 cut(s) 1235
SstI GAGCTC 1 cut(s) 2163
StyD4I CCNGG 4 cut(s) 365, 1763, 2056, 2840
StyI CCWWGG 3 cut(s) 1561, 1665, 2545
TaaI ACNGT 3 cut(s) 1128, 2812, 2949
TaiI ACGT 2 cut(s) 847, 1140
TaqI TCGA 3 cut(s) 1375, 2785, 3012
TatI WGTACW 5 cut(s) 745, 1494, 1836, 2086, 2804
TfiI GAWTC 7 cut(s) 380, 519, 715, 1780, 1843, 2314, 3155
TscAI CASTG 6 cut(s) 1221, 1585, 2003, 2754, 2817, 2985
TseFI GTSAC 3 cut(s) 1063, 1673, 2263
TseI GCWGC 8 cut(s) 193, 1357, 2280, 2723, 2972, 2975, 3095, 3193
Tsp45I GTSAC 3 cut(s) 1063, 1673, 2263
TspGWI ACGGA 2 cut(s) 1070, 1833
TspRI CASTG 6 cut(s) 1221, 1585, 2003, 2754, 2817, 2985
VpaK11BI GGWCC 4 cut(s) 111, 1396, 1483, 2684
XagI CCTNNNNNAGG 1 cut(s) 2055
XapI RAATTY 8 cut(s) 119, 1152, 1388, 1810, 1827, 1905, 2909, 3055
XbaI TCTAGA 1 cut(s) 1516
XceI RCATGY 2 cut(s) 752, 2730
XcmI CCANNNNNNNNNTGG 6 cut(s) 373, 391, 575, 1006, 2475, 2599
XmiI GTMKAC 2 cut(s) 1123, 1547
XmnI GAANNNNTTC 4 cut(s) 1746, 1773, 1830, 2444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.