RchiOBHm_Chr2g0130521

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003486
Physical Location & Seq
Reverse (-)
46565095 .. 46568128
3034 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50198

Sequence Viewer

Length: 546 bp
ATGCAACTGGAATTTTTTTTAGTGCTTTTGTTGCGGTTTAGAGAAAGTCTCAAGGGTGAAATATGCATCTTCTTTCCTTTGATAGTTCTCCGGTCCCTGGATGGCTTGGATTTACCCATGAACCAAAAAATGATTGTCCTTAGGATGGTTGAAAAGGTTTGCAAGGATCTTCAGATGCTTGTTGACATATTTATGAACTATGACTGTGATCTTGAGGCACTGAACTTGTTTGAGCGGATGAATATCTCAAGGGATGCAAAATGCAGATCCAATATGGCTGTTGCGTCCCAGACTACTTCAATAAAGGGTTCATCTCTTCTGTGTCTTGTGAACGTGCTTAAATCACTTGTAGATTGGAAAAAGTCATGCGGAGAATCAGATAGAATAAAAACATTCAGTCTCTGGAAGGAGATGCTTCAGCTAAAGAGTGTTGACGTGAAAAGCAGGCAAGATGCAACCATTAACTTTGAGAAGGCAAAAGCTCATAAGTCTCTACAGTGGAATCTGCCATATTTGAGGTGGAATAAAGTGATGTTGTATTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0002376 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0040007 GO:0042742 GO:0043086 GO:0043087 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0051704 GO:0051707 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0097708 GO:0098542 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

181

Amino Acids

21.22

Weight (kDa)

9.14

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7-like_HUS PF12783 3 - 75 6.6e-18 Mon2/Sec7/BIG1-like, HUS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 235
AciI CCGC 3 cut(s) 34, 235, 369
AclWI GGATC 2 cut(s) 174, 261
AcsI RAATTY 1 cut(s) 11
AcuI CTGAAG 2 cut(s) 155, 401
AfiI CCNNNNNNNGG 2 cut(s) 97, 145
AgsI TTSAA 2 cut(s) 152, 300
AjiI CACGTC 1 cut(s) 436
AjnI CCWGG 1 cut(s) 96
AluBI AGCT 2 cut(s) 421, 482
AluI AGCT 2 cut(s) 421, 482
Alw26I GTCTC 3 cut(s) 53, 404, 495
AlwI GGATC 2 cut(s) 174, 261
AlwNI CAGNNNCTG 1 cut(s) 402
ApoI RAATTY 1 cut(s) 11
AspS9I GGNCC 1 cut(s) 93
AsuHPI GGTGA 1 cut(s) 68
AvaII GGWCC 1 cut(s) 93
AxyI CCTNAGG 1 cut(s) 140
BccI CCATC 2 cut(s) 95, 139
BciT130I CCWGG 1 cut(s) 98
BcoDI GTCTC 3 cut(s) 53, 404, 495
BfmI CTRYAG 1 cut(s) 494
Bme1390I CCNGG 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 93
BmgBI CACGTC 1 cut(s) 436
BmgT120I GGNCC 1 cut(s) 93
BmiI GGNNCC 1 cut(s) 95
BmrFI CCNGG 1 cut(s) 98
BmsI GCATC 5 cut(s) 75, 165, 244, 402, 442
BplI GAGNNNNNCTC 2 cut(s) 33, 65
BpuEI CTTGAG 3 cut(s) 35, 232, 233
BsaBI GATNNNNATC 1 cut(s) 242
BsaJI CCNNGG 1 cut(s) 96
BsaWI WCCGGW 1 cut(s) 90
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 145
Bse1I ACTGG 1 cut(s) 12
Bse21I CCTNAGG 1 cut(s) 140
Bse8I GATNNNNATC 1 cut(s) 242
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 4 cut(s) 106, 150, 243, 259
BseJI GATNNNNATC 1 cut(s) 242
BseLI CCNNNNNNNGG 2 cut(s) 97, 145
BseNI ACTGG 1 cut(s) 12
BsiSI CCGG 1 cut(s) 91
BslFI GGGAC 2 cut(s) 79, 271
BslI CCNNNNNNNGG 2 cut(s) 97, 145
BsmAI GTCTC 3 cut(s) 53, 404, 495
BsmFI GGGAC 2 cut(s) 79, 271
Bsp143I GATC 3 cut(s) 166, 208, 266
BspACI CCGC 3 cut(s) 34, 235, 369
BspLI GGNNCC 1 cut(s) 95
BspPI GGATC 2 cut(s) 174, 261
BsrBI CCGCTC 1 cut(s) 235
BsrI ACTGG 1 cut(s) 12
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 3 cut(s) 166, 208, 266
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 2 cut(s) 206, 498
Bst6I CTCTTC 1 cut(s) 321
BstC8I GCNNGC 1 cut(s) 446
BstDEI CTNAG 1 cut(s) 140
BstF5I GGATG 4 cut(s) 106, 150, 243, 259
BstKTI GATC 3 cut(s) 169, 211, 269
BstMAI GTCTC 3 cut(s) 53, 404, 495
BstMBI GATC 3 cut(s) 166, 208, 266
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstNI CCWGG 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 96
BstSFI CTRYAG 1 cut(s) 494
BstX2I RGATCY 2 cut(s) 166, 266
BstYI RGATCY 2 cut(s) 166, 266
Bsu36I CCTNAGG 1 cut(s) 140
BtrI CACGTC 1 cut(s) 436
BtsCI GGATG 4 cut(s) 106, 150, 243, 259
BtsIMutI CAGTG 2 cut(s) 218, 503
Cac8I GCNNGC 1 cut(s) 446
CaiI CAGNNNCTG 1 cut(s) 402
Cfr13I GGNCC 1 cut(s) 93
CseI GACGC 1 cut(s) 273
CviAII CATG 2 cut(s) 118, 366
CviJI RGCY 4 cut(s) 105, 278, 421, 482
CviKI_1 RGCY 4 cut(s) 105, 278, 421, 482
DdeI CTNAG 1 cut(s) 140
DpnI GATC 3 cut(s) 168, 210, 268
DpnII GATC 3 cut(s) 166, 208, 266
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 93
Eco57I CTGAAG 2 cut(s) 155, 401
Eco81I CCTNAGG 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 96
EcoT22I ATGCAT 1 cut(s) 68
FaeI CATG 2 cut(s) 121, 369
FaiI YATR 9 cut(s) 64, 119, 188, 194, 201, 275, 367, 486, 511
FaqI GGGAC 2 cut(s) 79, 271
FatI CATG 2 cut(s) 117, 365
FokI GGATG 4 cut(s) 113, 157, 250, 266
HapII CCGG 1 cut(s) 91
HgaI GACGC 1 cut(s) 273
Hin1II CATG 2 cut(s) 121, 369
HincII GTYRAC 2 cut(s) 184, 433
HindII GTYRAC 2 cut(s) 184, 433
HinfI GANTC 2 cut(s) 374, 502
HpaII CCGG 1 cut(s) 91
HphI GGTGA 1 cut(s) 68
Hpy166II GTNNAC 3 cut(s) 184, 331, 433
Hpy188I TCNGA 2 cut(s) 174, 379
Hpy188III TCNNGA 2 cut(s) 212, 403
Hpy8I GTNNAC 3 cut(s) 184, 331, 433
HpyAV CCTTC 2 cut(s) 400, 466
HpyCH4III ACNGT 2 cut(s) 206, 498
HpyCH4IV ACGT 2 cut(s) 333, 435
HpyCH4V TGCA 6 cut(s) 4, 66, 162, 257, 264, 455
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpyF3I CTNAG 1 cut(s) 140
HpySE526I ACGT 2 cut(s) 333, 435
Hsp92II CATG 2 cut(s) 121, 369
Kzo9I GATC 3 cut(s) 166, 208, 266
LpnPI CCDG 6 cut(s) 83, 104, 110, 302, 388, 430
LweI GCATC 5 cut(s) 75, 165, 244, 402, 442
MaeII ACGT 2 cut(s) 333, 435
MalI GATC 3 cut(s) 168, 210, 268
MbiI CCGCTC 1 cut(s) 235
MboI GATC 3 cut(s) 166, 208, 266
MboII GAAGA 3 cut(s) 61, 161, 308
MflI RGATCY 2 cut(s) 166, 266
MluCI AATT 1 cut(s) 11
MnlI CCTC 2 cut(s) 208, 510
Mph1103I ATGCAT 1 cut(s) 68
MseI TTAA 2 cut(s) 339, 462
MslI CAYNNNNRTG 1 cut(s) 191
MspI CCGG 1 cut(s) 91
MspR9I CCNGG 1 cut(s) 98
MvaI CCWGG 1 cut(s) 98
MwoI GCNNNNNNNGC 1 cut(s) 31
NdeII GATC 3 cut(s) 166, 208, 266
NlaIII CATG 2 cut(s) 121, 369
NlaIV GGNNCC 1 cut(s) 95
NsiI ATGCAT 1 cut(s) 68
PfeI GAWTC 2 cut(s) 374, 502
Psp6I CCWGG 1 cut(s) 96
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 1 cut(s) 95
PspPI GGNCC 1 cut(s) 93
PstNI CAGNNNCTG 1 cut(s) 402
PsuI RGATCY 2 cut(s) 166, 266
RseI CAYNNNNRTG 1 cut(s) 191
SaqAI TTAA 2 cut(s) 339, 462
Sau3AI GATC 3 cut(s) 166, 208, 266
Sau96I GGNCC 1 cut(s) 93
ScrFI CCNGG 1 cut(s) 98
SetI ASST 6 cut(s) 159, 336, 423, 438, 484, 521
SfaNI GCATC 5 cut(s) 75, 165, 244, 402, 442
SfcI CTRYAG 1 cut(s) 494
SinI GGWCC 1 cut(s) 93
SmiMI CAYNNNNRTG 1 cut(s) 191
SmlI CTYRAG 3 cut(s) 50, 212, 247
SmoI CTYRAG 3 cut(s) 50, 212, 247
Sse9I AATT 1 cut(s) 11
SsiI CCGC 3 cut(s) 34, 235, 369
StyD4I CCNGG 1 cut(s) 96
TaaI ACNGT 2 cut(s) 206, 498
TaiI ACGT 2 cut(s) 336, 438
TasI AATT 1 cut(s) 11
TfiI GAWTC 2 cut(s) 374, 502
Tru1I TTAA 2 cut(s) 339, 462
Tru9I TTAA 2 cut(s) 339, 462
TscAI CASTG 2 cut(s) 225, 503
TspDTI ATGAA 4 cut(s) 134, 209, 254, 300
TspRI CASTG 2 cut(s) 225, 503
VpaK11BI GGWCC 1 cut(s) 93
XapI RAATTY 1 cut(s) 11
XcmI CCANNNNNNNNNTGG 1 cut(s) 516
Zsp2I ATGCAT 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.