Rorug01G0360800

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003486
Physical Location & Seq
Reverse (-)
47631217 .. 47649244
18028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0360800.1

Sequence Viewer

Length: 996 bp
ATGAGGAACATGACTGCAATTCTTGTCATTGCAGTGTGTTTTGTTCAAGCCTTGTATGCAGGCCTTTCCATCATCTCATTCCTTGCACTAAAGCAGGGCATGAGCCACTACACCTTTGTGGTGTATAGGATGGCCATTGCAACTGCCTTGGCAATTCCATTGGCATGGTATATAGAGAGACATTCTAGGCCTACAATGACCATCAAGGTCATGGCAAAGATCATGCTGCTAAGCATGTTTGACCCTGTAATGGACCAAAACTTATACTATGTGGGAATGAAGAACTCCAATGCCACATTCACATCGGCCATGTGCAACATGCTTCCAGTGTTTGCGTTTGTCATGGCCTGGATTTTCAGACACCCGAGAGGATTGACCAAGGTTGTAGGAACCTTGGTTACTGTGGAAGGAGCAATCCTCCTTACCATGGTCAAGGGGCCTTCCCTCAACTTGCCATGGGCAAGGGACCACGACAAAAGTCATTCTTCAGCGCCCAAAGTCAAGGGTGCTCTCTTCCTAACACTAGCCTGCTTCTGCTGGTCCTGTTTCATAATCATGCAAGCCAATGTACTCAAGTCCTACCCCTGCAAGCTCTCTCTCACCGCTTTGATTTGCTTCTGGGGTATGGTGGAAGGAGCAGTGGTGGCTGTTGTGGTTGAAAGGGGGAATTTGGAAGCATGGTCCATACACTTGGACTTGAAGTTGCTCGCAGCTGTTTATGGGGCTCTTGTATCGGGGGCTGCATATTATGTCATGGGACTGGTTGTGAAGAAGAAGGGACCAGTTTTCTACTCGGCCTTTAACCCCTTGGCCACTCTACTTGTGGCGATTTTGGGGTCATTTTTTCTGCATGAACAATTGTATACTGGGAGTCTCATCGGAGTTGTAACCATTATGGGAGGCCTCTATCTTGTTCTTTGGGGAAAAGCAAGAGATCAACCTCCCTTAAATTCAAAGGATGCCAATTTGGTCTACCAAACCGAACTCCAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0002376 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0040007 GO:0042742 GO:0043086 GO:0043087 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0051704 GO:0051707 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0097708 GO:0098542 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

331

Amino Acids

36.5

Weight (kDa)

9.38

Isoelectric Point (pI)

25.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 10 - 142 4.9e-12 EamA-like transporter family
EamA PF00892 168 - 306 2.7e-09 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 863, 972
AciI CCGC 1 cut(s) 603
AcoI YGGCCR 3 cut(s) 132, 306, 810
AcsI RAATTY 2 cut(s) 667, 949
AcuI CTGAAG 1 cut(s) 471
AfaI GTAC 1 cut(s) 570
AfiI CCNNNNNNNGG 2 cut(s) 250, 427
AgsI TTSAA 4 cut(s) 47, 659, 700, 954
AjnI CCWGG 1 cut(s) 347
AjuI GAANNNNNNNTTGG 2 cut(s) 281, 313
AleI CACNNNNGTG 1 cut(s) 116
AluBI AGCT 2 cut(s) 592, 713
AluI AGCT 2 cut(s) 592, 713
Alw21I GWGCWC 1 cut(s) 511
Alw26I GTCTC 2 cut(s) 172, 878
Ama87I CYCGRG 1 cut(s) 364
AoxI GGCC 9 cut(s) 61, 132, 188, 306, 345, 437, 795, 810, 901
ApeKI GCWGC 3 cut(s) 226, 710, 740
ApoI RAATTY 2 cut(s) 667, 949
AspLEI GCGC 1 cut(s) 493
AspS9I GGNCC 6 cut(s) 253, 437, 466, 540, 681, 779
AsuHPI GGTGA 1 cut(s) 592
AvaI CYCGRG 1 cut(s) 364
AvaII GGWCC 5 cut(s) 253, 466, 540, 681, 779
BalI TGGCCA 2 cut(s) 134, 812
BanII GRGCYC 1 cut(s) 727
Bbv12I GWGCWC 1 cut(s) 511
BbvI GCAGC 3 cut(s) 213, 722, 727
BccI CCATC 3 cut(s) 77, 124, 209
BciT130I CCWGG 1 cut(s) 349
BcoDI GTCTC 2 cut(s) 172, 878
BfaI CTAG 2 cut(s) 186, 524
BfoI RGCGCY 1 cut(s) 494
BisI GCNGC 3 cut(s) 227, 711, 741
BlpI GCTNAGC 1 cut(s) 230
BlsI GCNGC 3 cut(s) 228, 712, 742
Bme1390I CCNGG 1 cut(s) 349
Bme18I GGWCC 5 cut(s) 253, 466, 540, 681, 779
BmeT110I CYCGRG 1 cut(s) 364
BmgT120I GGNCC 6 cut(s) 253, 437, 466, 540, 681, 779
BmiI GGNNCC 4 cut(s) 391, 438, 467, 780
BmrFI CCNGG 1 cut(s) 349
BmrI ACTGGG 1 cut(s) 876
BmsI GCATC 1 cut(s) 949
BmuI ACTGGG 1 cut(s) 876
BoxI GACNNNNGTC 1 cut(s) 477
BplI GAGNNNNNCTC 2 cut(s) 402, 434
BpmI CTGGAG 1 cut(s) 971
Bpu1102I GCTNAGC 1 cut(s) 230
BpuEI CTTGAG 1 cut(s) 557
BsaJI CCNNGG 6 cut(s) 147, 378, 393, 426, 455, 807
BsaXI ACNNNNNCTCC 2 cut(s) 627, 657
Bsc4I CCNNNNNNNGG 2 cut(s) 250, 427
Bse1I ACTGG 4 cut(s) 326, 765, 782, 871
Bse3DI GCAATG 2 cut(s) 27, 135
BseBI CCWGG 1 cut(s) 349
BseDI CCNNGG 6 cut(s) 147, 378, 393, 426, 455, 807
BseGI GGATG 2 cut(s) 135, 964
BseLI CCNNNNNNNGG 2 cut(s) 250, 427
BseMI GCAATG 2 cut(s) 27, 135
BseNI ACTGG 4 cut(s) 326, 765, 782, 871
BseXI GCAGC 3 cut(s) 213, 722, 727
BshFI GGCC 9 cut(s) 63, 134, 190, 308, 347, 439, 797, 812, 903
BsiHKAI GWGCWC 1 cut(s) 511
BsiHKCI CYCGRG 1 cut(s) 364
BslFI GGGAC 3 cut(s) 479, 771, 792
BslI CCNNNNNNNGG 2 cut(s) 250, 427
BsmAI GTCTC 2 cut(s) 172, 878
BsmFI GGGAC 3 cut(s) 479, 771, 792
BsnI GGCC 9 cut(s) 63, 134, 190, 308, 347, 439, 797, 812, 903
BsoBI CYCGRG 1 cut(s) 364
Bsp1286I GDGCHC 2 cut(s) 511, 727
Bsp143I GATC 2 cut(s) 219, 934
Bsp1720I GCTNAGC 1 cut(s) 230
Bsp19I CCATGG 2 cut(s) 426, 455
BspACI CCGC 1 cut(s) 603
BspANI GGCC 9 cut(s) 63, 134, 190, 308, 347, 439, 797, 812, 903
BspLI GGNNCC 4 cut(s) 391, 438, 467, 780
BsrDI GCAATG 2 cut(s) 27, 135
BsrI ACTGG 4 cut(s) 326, 765, 782, 871
BssECI CCNNGG 6 cut(s) 147, 378, 393, 426, 455, 807
BssMI GATC 2 cut(s) 219, 934
BssNAI GTATAC 1 cut(s) 864
BssT1I CCWWGG 6 cut(s) 147, 378, 393, 426, 455, 807
Bst1107I GTATAC 1 cut(s) 864
Bst2UI CCWGG 1 cut(s) 349
Bst4CI ACNGT 1 cut(s) 403
Bst6I CTCTTC 1 cut(s) 518
BstC8I GCNNGC 5 cut(s) 61, 529, 561, 590, 708
BstDEI CTNAG 1 cut(s) 230
BstDSI CCRYGG 2 cut(s) 426, 455
BstF5I GGATG 2 cut(s) 135, 964
BstH2I RGCGCY 1 cut(s) 494
BstHHI GCGC 1 cut(s) 493
BstKTI GATC 2 cut(s) 222, 937
BstMAI GTCTC 2 cut(s) 172, 878
BstMBI GATC 2 cut(s) 219, 934
BstMWI GCNNNNNNNGC 2 cut(s) 56, 644
BstNI CCWGG 1 cut(s) 349
BstNSI RCATGY 2 cut(s) 238, 322
BstPAI GACNNNNGTC 1 cut(s) 477
BstSCI CCNGG 1 cut(s) 347
BstV1I GCAGC 3 cut(s) 213, 722, 727
BstXI CCANNNNNNTGG 2 cut(s) 165, 691
BstZ17I GTATAC 1 cut(s) 864
BsuRI GGCC 9 cut(s) 63, 134, 190, 308, 347, 439, 797, 812, 903
BtgI CCRYGG 2 cut(s) 426, 455
BtsCI GGATG 2 cut(s) 135, 964
BtsI GCAGTG 2 cut(s) 39, 645
BtsIMutI CAGTG 3 cut(s) 39, 333, 645
Cac8I GCNNGC 5 cut(s) 61, 529, 561, 590, 708
CfoI GCGC 1 cut(s) 493
Cfr13I GGNCC 6 cut(s) 253, 437, 466, 540, 681, 779
Csp6I GTAC 1 cut(s) 569
CspCI CAANNNNNGTGG 2 cut(s) 802, 837
CviQI GTAC 1 cut(s) 569
DdeI CTNAG 1 cut(s) 230
DpnI GATC 2 cut(s) 221, 936
DpnII GATC 2 cut(s) 219, 934
EaeI YGGCCR 3 cut(s) 132, 306, 810
Eam1104I CTCTTC 1 cut(s) 518
EarI CTCTTC 1 cut(s) 518
Eco130I CCWWGG 6 cut(s) 147, 378, 393, 426, 455, 807
Eco147I AGGCCT 3 cut(s) 63, 190, 903
Eco24I GRGCYC 1 cut(s) 727
Eco47I GGWCC 5 cut(s) 253, 466, 540, 681, 779
Eco57I CTGAAG 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 364
EcoO109I RGGNCCY 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 347
EcoT14I CCWWGG 6 cut(s) 147, 378, 393, 426, 455, 807
EcoT38I GRGCYC 1 cut(s) 727
ErhI CCWWGG 6 cut(s) 147, 378, 393, 426, 455, 807
FalI AAGNNNNNCTT 2 cut(s) 469, 501
FaqI GGGAC 3 cut(s) 479, 771, 792
FblI GTMKAC 2 cut(s) 863, 972
Fnu4HI GCNGC 3 cut(s) 227, 711, 741
FokI GGATG 2 cut(s) 142, 971
FriOI GRGCYC 1 cut(s) 727
Fsp4HI GCNGC 3 cut(s) 227, 711, 741
FspBI CTAG 2 cut(s) 186, 524
GlaI GCGC 1 cut(s) 492
GluI GCNGC 3 cut(s) 227, 711, 741
GsuI CTGGAG 1 cut(s) 971
HaeII RGCGCY 1 cut(s) 494
HaeIII GGCC 9 cut(s) 63, 134, 190, 308, 347, 439, 797, 812, 903
HhaI GCGC 1 cut(s) 493
Hin6I GCGC 1 cut(s) 491
HinP1I GCGC 1 cut(s) 491
HinfI GANTC 1 cut(s) 871
HphI GGTGA 1 cut(s) 592
Hpy166II GTNNAC 2 cut(s) 864, 973
Hpy188I TCNGA 2 cut(s) 359, 881
Hpy8I GTNNAC 2 cut(s) 864, 973
HpyAV CCTTC 4 cut(s) 401, 450, 626, 769
HpyCH4III ACNGT 1 cut(s) 403
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 644
HpyF3I CTNAG 1 cut(s) 230
HspAI GCGC 1 cut(s) 491
Kzo9I GATC 2 cut(s) 219, 934
LmnI GCTCC 2 cut(s) 410, 635
Lsp1109I GCAGC 3 cut(s) 213, 722, 727
LweI GCATC 1 cut(s) 949
MaeI CTAG 2 cut(s) 186, 524
MaeIII GTNAC 2 cut(s) 397, 886
MalI GATC 2 cut(s) 221, 936
MboI GATC 2 cut(s) 219, 934
MboII GAAGA 5 cut(s) 292, 477, 505, 781, 784
MfeI CAATTG 1 cut(s) 857
MhlI GDGCHC 2 cut(s) 511, 727
MlsI TGGCCA 2 cut(s) 134, 812
MluCI AATT 6 cut(s) 18, 153, 667, 857, 949, 964
MluNI TGGCCA 2 cut(s) 134, 812
MlyI GAGTC 1 cut(s) 880
MnlI CCTC 6 cut(s) 362, 428, 455, 893, 914, 951
Mox20I TGGCCA 2 cut(s) 134, 812
MscI TGGCCA 2 cut(s) 134, 812
MseI TTAA 2 cut(s) 801, 947
MslI CAYNNNNRTG 4 cut(s) 32, 116, 163, 554
Msp20I TGGCCA 2 cut(s) 134, 812
MspA1I CMGCKG 1 cut(s) 713
MspR9I CCNGG 1 cut(s) 349
MunI CAATTG 1 cut(s) 857
MvaI CCWGG 1 cut(s) 349
MwoI GCNNNNNNNGC 2 cut(s) 56, 644
NcoI CCATGG 2 cut(s) 426, 455
NdeII GATC 2 cut(s) 219, 934
NlaIV GGNNCC 4 cut(s) 391, 438, 467, 780
NmeAIII GCCGAG 1 cut(s) 773
NspI RCATGY 2 cut(s) 238, 322
OliI CACNNNNGTG 1 cut(s) 116
PceI AGGCCT 3 cut(s) 63, 190, 903
PkrI GCNGC 3 cut(s) 228, 712, 742
PleI GAGTC 1 cut(s) 879
PpsI GAGTC 1 cut(s) 879
PshAI GACNNNNGTC 1 cut(s) 477
Psp6I CCWGG 1 cut(s) 347
PspGI CCWGG 1 cut(s) 347
PspN4I GGNNCC 4 cut(s) 391, 438, 467, 780
PspPI GGNCC 6 cut(s) 253, 437, 466, 540, 681, 779
PsrI GAACNNNNNNTAC 2 cut(s) 382, 414
PvuII CAGCTG 1 cut(s) 713
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
RseI CAYNNNNRTG 4 cut(s) 32, 116, 163, 554
SaqAI TTAA 2 cut(s) 801, 947
SatI GCNGC 3 cut(s) 227, 711, 741
Sau3AI GATC 2 cut(s) 219, 934
Sau96I GGNCC 6 cut(s) 253, 437, 466, 540, 681, 779
SchI GAGTC 1 cut(s) 880
ScrFI CCNGG 1 cut(s) 349
SduI GDGCHC 2 cut(s) 511, 727
SetI ASST 7 cut(s) 116, 210, 384, 395, 594, 715, 943
SfaNI GCATC 1 cut(s) 949
SinI GGWCC 5 cut(s) 253, 466, 540, 681, 779
SmiMI CAYNNNNRTG 4 cut(s) 32, 116, 163, 554
SmlI CTYRAG 1 cut(s) 572
SmoI CTYRAG 1 cut(s) 572
Sse9I AATT 6 cut(s) 18, 153, 667, 857, 949, 964
SseBI AGGCCT 3 cut(s) 63, 190, 903
SsiI CCGC 1 cut(s) 603
SspMI CTAG 2 cut(s) 186, 524
StuI AGGCCT 3 cut(s) 63, 190, 903
StyD4I CCNGG 1 cut(s) 347
StyI CCWWGG 6 cut(s) 147, 378, 393, 426, 455, 807
TaaI ACNGT 1 cut(s) 403
TasI AATT 6 cut(s) 18, 153, 667, 857, 949, 964
TatI WGTACW 1 cut(s) 568
Tru1I TTAA 2 cut(s) 801, 947
Tru9I TTAA 2 cut(s) 801, 947
TscAI CASTG 3 cut(s) 39, 333, 645
TseI GCWGC 3 cut(s) 226, 710, 740
TspDTI ATGAA 3 cut(s) 293, 538, 867
TspRI CASTG 3 cut(s) 39, 333, 645
VpaK11BI GGWCC 5 cut(s) 253, 466, 540, 681, 779
XapI RAATTY 2 cut(s) 667, 949
XceI RCATGY 2 cut(s) 238, 322
XcmI CCANNNNNNNNNTGG 2 cut(s) 208, 820
XmiI GTMKAC 2 cut(s) 863, 972
XspI CTAG 2 cut(s) 186, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.