Rorug04G0057500

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003486
Physical Location & Seq
Forward (+)
9005246 .. 9005671
426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0057500.1

Sequence Viewer

Length: 426 bp
ATGGGTTTAATATTTCGCTACCAAGCTTTGCTGCTCGCCTTGTTCATTCCTTTCTTTGTGGCGCTTACAACTGCTGTGGTACCCTTCAAAGTCGTGACCTCGATCGACAACACATTACCTGATGGGCAAGAACTCAATGTCCACTGTAAATCCGGGGACGACGATCTAGGCGCTCAGGTCATACCCAATGGAGGTCATTACGAATGGAGCTTCAAAGTTAATGTATGGGCAACAACGCTTTTCTTCTGTTCATTTAATTGCAAAGACGGATCAGGAACATTTGACATCTACAGAGCCAGTCGGGATATCATTAGATGTGAAGACAAGTGCAACTGGAAAGCTGCCCAAGATGGCGTGCATGGCTTCAACAAAGACAATCAGGATGACTTTCTGTACAAGTGGGAGGCACCACAAGTGAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0002376 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0040007 GO:0042742 GO:0043086 GO:0043087 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0051704 GO:0051707 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0097708 GO:0098542 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

141

Amino Acids

15.93

Weight (kDa)

5.21

Isoelectric Point (pI)

15.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 35 - 134 1.3e-29 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 79
AccB1I GGYRCC 2 cut(s) 79, 406
AclWI GGATC 1 cut(s) 277
AfaI GTAC 2 cut(s) 81, 395
AfiI CCNNNNNNNGG 1 cut(s) 191
AgsI TTSAA 3 cut(s) 88, 214, 367
AloI GAACNNNNNNTCC 2 cut(s) 123, 155
AluBI AGCT 3 cut(s) 26, 210, 341
AluI AGCT 3 cut(s) 26, 210, 341
AlwI GGATC 1 cut(s) 277
ApeKI GCWGC 2 cut(s) 31, 341
Asp718I GGTACC 1 cut(s) 79
AspLEI GCGC 2 cut(s) 64, 173
AsuC2I CCSGG 1 cut(s) 154
BanI GGYRCC 2 cut(s) 79, 406
BbsI GAAGAC 1 cut(s) 327
BbvI GCAGC 2 cut(s) 18, 328
BccI CCATC 2 cut(s) 116, 344
BcnI CCSGG 1 cut(s) 154
BfaI CTAG 1 cut(s) 167
BfmI CTRYAG 1 cut(s) 289
BfoI RGCGCY 2 cut(s) 65, 174
BisI GCNGC 2 cut(s) 32, 342
BlsI GCNGC 2 cut(s) 33, 343
Bme1390I CCNGG 1 cut(s) 154
BmiI GGNNCC 2 cut(s) 81, 408
BmrFI CCNGG 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 327
Bpu10I CCTNAGC 1 cut(s) 174
BpuMI CCSGG 1 cut(s) 154
BsaJI CCNNGG 1 cut(s) 153
Bsc4I CCNNNNNNNGG 1 cut(s) 191
Bse1I ACTGG 2 cut(s) 297, 338
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 1 cut(s) 388
BseLI CCNNNNNNNGG 1 cut(s) 191
BseMII CTCAG 1 cut(s) 188
BseNI ACTGG 2 cut(s) 297, 338
BseXI GCAGC 2 cut(s) 18, 328
Bsh1285I CGRYCG 1 cut(s) 105
BshNI GGYRCC 2 cut(s) 79, 406
BsiEI CGRYCG 1 cut(s) 105
BsiSI CCGG 1 cut(s) 153
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 170
Bsp1407I TGTACA 1 cut(s) 393
Bsp143I GATC 3 cut(s) 102, 163, 269
BspCNI CTCAG 1 cut(s) 187
BspLI GGNNCC 2 cut(s) 81, 408
BspPI GGATC 1 cut(s) 277
BspT107I GGYRCC 2 cut(s) 79, 406
BsrGI TGTACA 1 cut(s) 393
BsrI ACTGG 2 cut(s) 297, 338
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 3 cut(s) 102, 163, 269
Bst4CI ACNGT 1 cut(s) 146
BstAUI TGTACA 1 cut(s) 393
BstC8I GCNNGC 2 cut(s) 36, 356
BstDEI CTNAG 1 cut(s) 174
BstF5I GGATG 1 cut(s) 388
BstH2I RGCGCY 2 cut(s) 65, 174
BstHHI GCGC 2 cut(s) 64, 173
BstKTI GATC 3 cut(s) 105, 166, 272
BstMBI GATC 3 cut(s) 102, 163, 269
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 1 cut(s) 360
BstSCI CCNGG 1 cut(s) 152
BstSFI CTRYAG 1 cut(s) 289
BstV1I GCAGC 2 cut(s) 18, 328
BstV2I GAAGAC 1 cut(s) 327
BtsCI GGATG 1 cut(s) 388
BtsIMutI CAGTG 1 cut(s) 142
Cac8I GCNNGC 2 cut(s) 36, 356
CfoI GCGC 2 cut(s) 64, 173
Csp6I GTAC 2 cut(s) 80, 394
CspCI CAANNNNNGTGG 2 cut(s) 57, 92
CviAII CATG 1 cut(s) 359
CviJI RGCY 5 cut(s) 26, 210, 296, 341, 363
CviKI_1 RGCY 5 cut(s) 26, 210, 296, 341, 363
CviQI GTAC 2 cut(s) 80, 394
DdeI CTNAG 1 cut(s) 174
DpnI GATC 3 cut(s) 104, 165, 271
DpnII GATC 3 cut(s) 102, 163, 269
Eco32I GATATC 1 cut(s) 307
EcoRV GATATC 1 cut(s) 307
FaeI CATG 1 cut(s) 362
FaiI YATR 4 cut(s) 182, 226, 360, 424
FaqI GGGAC 1 cut(s) 170
FatI CATG 1 cut(s) 358
Fnu4HI GCNGC 2 cut(s) 32, 342
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 2 cut(s) 32, 342
FspBI CTAG 1 cut(s) 167
GlaI GCGC 2 cut(s) 63, 172
GluI GCNGC 2 cut(s) 32, 342
HaeII RGCGCY 2 cut(s) 65, 174
HapII CCGG 1 cut(s) 153
HhaI GCGC 2 cut(s) 64, 173
Hin1II CATG 1 cut(s) 362
Hin6I GCGC 2 cut(s) 62, 171
HinP1I GCGC 2 cut(s) 62, 171
HindIII AAGCTT 1 cut(s) 24
HpaII CCGG 1 cut(s) 153
Hpy166II GTNNAC 1 cut(s) 142
Hpy188III TCNNGA 4 cut(s) 94, 273, 302, 380
Hpy8I GTNNAC 1 cut(s) 142
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 1 cut(s) 146
HpyCH4V TGCA 3 cut(s) 261, 330, 358
HpyF10VI GCNNNNNNNGC 1 cut(s) 360
HpyF3I CTNAG 1 cut(s) 174
Hsp92II CATG 1 cut(s) 362
HspAI GCGC 2 cut(s) 62, 171
KpnI GGTACC 1 cut(s) 83
Kzo9I GATC 3 cut(s) 102, 163, 269
LmnI GCTCC 1 cut(s) 207
LpnPI CCDG 7 cut(s) 132, 161, 166, 258, 310, 319, 365
Lsp1109I GCAGC 2 cut(s) 18, 328
MaeI CTAG 1 cut(s) 167
MaeIII GTNAC 1 cut(s) 94
MalI GATC 3 cut(s) 104, 165, 271
MboI GATC 3 cut(s) 102, 163, 269
MboII GAAGA 2 cut(s) 235, 332
MluCI AATT 1 cut(s) 256
MnlI CCTC 3 cut(s) 109, 185, 397
MseI TTAA 3 cut(s) 8, 219, 255
MspI CCGG 1 cut(s) 153
MspR9I CCNGG 1 cut(s) 154
MwoI GCNNNNNNNGC 1 cut(s) 360
NciI CCSGG 1 cut(s) 154
NdeII GATC 3 cut(s) 102, 163, 269
NlaIII CATG 1 cut(s) 362
NlaIV GGNNCC 2 cut(s) 81, 408
NmuCI GTSAC 1 cut(s) 94
PkrI GCNGC 2 cut(s) 33, 343
Ple19I CGATCG 1 cut(s) 105
PspN4I GGNNCC 2 cut(s) 81, 408
PvuI CGATCG 1 cut(s) 105
RsaI GTAC 2 cut(s) 81, 395
RsaNI GTAC 2 cut(s) 80, 394
SaqAI TTAA 3 cut(s) 8, 219, 255
SatI GCNGC 2 cut(s) 32, 342
Sau3AI GATC 3 cut(s) 102, 163, 269
ScrFI CCNGG 1 cut(s) 154
SetI ASST 7 cut(s) 28, 101, 121, 180, 196, 212, 343
SfcI CTRYAG 1 cut(s) 289
Sse9I AATT 1 cut(s) 256
SspI AATATT 1 cut(s) 12
SspMI CTAG 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 152
TaaI ACNGT 1 cut(s) 146
TaqI TCGA 2 cut(s) 101, 105
TasI AATT 1 cut(s) 256
TatI WGTACW 1 cut(s) 393
Tru1I TTAA 3 cut(s) 8, 219, 255
Tru9I TTAA 3 cut(s) 8, 219, 255
TscAI CASTG 1 cut(s) 149
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 2 cut(s) 31, 341
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 2 cut(s) 34, 240
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 1 cut(s) 149
XspI CTAG 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.